[Histonet] RE: peach stone (pit) sectioning?

[John Kiernan]

The book "Botanical Microtechnique and Cytochemistry" by Graeme P. Berlyn & Jerome P. Miksche (Iowas State University Press, 1976; ISBN 813802202) give a detailed account of softening wood using hydrofluoric acid - including safety precautions for this nasty stuff. Pages 73-74. A sliding microtome is recommended. This book also has a good chapter on cutting techniques for botanical materials. It is out-of-print but available 2nd-hand quite cheaply, eg http://www.amazon.com/s?ie=UTF8&page=1&rh=n%3A283155%2Cp_27%3AGraeme%20P.%3B%20Miksche%5Cc%20Jerome%20P.%20Berlyn

John Kiernan
London, Canada
= = =
On 10/02/15, "Preiszner, Johanna"  <PREISZNE <@t> mail.etsu.edu> wrote:
> Thanks everybody for the ideas and the expression of sympathy!
> 
> Our conclusion is that it's not possible to soften up a hard fruit pit for paraffin sectioning in a standard Histology lab. We never tried celloidin embedding, so that's still a possiblility.
> 
> The best approach might be a bone or mineral grinder that can produce microscopic sections of hard material.
> 
> Thanks again,
> 
> Hanna Preiszner
> ETSU/QCOM
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Preiszner, Johanna [PREISZNE <@t> mail.etsu.edu]
> Sent: Tuesday, February 03, 2015 4:20 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] peach stone (pit) sectioning?
> 
> Hi Netters,
> 
> does anybody know how I could soften up a hard fruit pit for sectioning?
> 
> Thanks,
> Hanna Preiszner
> ETSU/QCOM
> 
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