[Histonet] Rat Neutrophils

[John Kiernan]

Rat neutrophils are very easy to recognize without the expense of immunohistochemistry. 
Their nuclei are U- and O-shaped, not segmented like the nuclei of human neutrophils. They are easy to see with any common nuclear stain such as haemalum (and eosin if desired, but not too strongly). Even better is an azure-eosin (Lillie) method or a blood stain such as Giemsa. For sections of formaldehyde-fixed tissue the pH needs to be 4-5, not the 6.8 generally considered optimal for alcohol-fixed blood films. Sections ideally should be thick enough to include most of the cell and its nucleus - about 7um. 

John Kiernan
London, Canada 
= = =
On 04/02/15, "Swartwood, Steven J"  <Steven.Swartwood <@t> cshs.org> wrote:
> Hello again everyone,
> 
> I have a researcher requesting IHC staining for Rat Neutrophils. I've found a few papers on PubMed and a few antibodies that are specific for Rat neutrophils. The tissue being used is FFPE Rat pancreas and lungs. We are specifically looking for Rat neutrophils. There was no human neutrophils injected into the Rats.
> 
> I just wanted to see if anyone out there does this or has done this recently and what antibodies they used. A protocol would be greatly appreciated as well. So far I've found more anti-MPO antibodies, but only 1 of them has actual pictures of neutrophil staining in Rats. I was looking into Ly6G antibodies, but none really looked too spectacular.
> 
> Any and all information is greatly appreciated. I thank all you for your time/efforts for helping me now and the previous help I've received.
> 
> Steven Swartwood HT(ASCP)
> Cedars Sinai Medical Center
> steven.swartwood <@t> cshs.org
> 
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