[Histonet] decalcifying sheep knee joints

Fiona J Wright fiona.wright <@t> sheffield.ac.uk
Thu Feb 5 10:10:15 CST 2015

Can anyone help me out with a problem Im going to have in a few months? I
will be receiving some sheep joints for processing. They will need to be
decalcified in a manner that will leave the tissue suitable for
immunohistochemistry and special stains such as safranin O. How do I go
about this? I have previously only worked with mouse bones and decalcified
for several weeks in EDTA; this has always given me good histological
results, but I fear for sheep bones this methodology would take months.

Any advice gratefully received.

Fiona Wright

Dept of Infection and Immunity
University of Sheffield
K118, Medical School
Beech Hill Road
S10 2RX
fiona.wright <@t> sheffield.ac.uk
0114 271 2102
07769 334438

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