[Histonet] denatured alcohol

[Rene J Buesa]

You will have to start in 80% "ethanol" and you cannot assume the tissue is dehydrated enough.René J.  

     On Thursday, February 5, 2015 3:57 PM, Jorge A. Santiago-Blay <blayjorge <@t> gmail.com> wrote:
   

 Dear Histonetters:

The other day, someone sent me a sample in (allegedly) denaturated alcohol.
I asked the colleague to let me know what was the concentration of ethanol
in the sample and the answer was "about 80%". From the smell, I suspect the
rest is methanol.

I need to dehydrate the sample through an ethanol series. Could someone
recommend where should I get started? OK to begin at 80% or to assume that
the sample is dehydrated enough in this denaturated alcohol. Thereafter, I
will process the sample via a critical point dryer.  If you have any
suggestions, please email me directly at blayjorge <@t> gmail.com . Thank you.

Sincerely,

Jorge

Jorge A. Santiago-Blay, PhD
blaypublishers.com
http://blayjorge.wordpress.com/
http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm
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