[Histonet] PAX-5 staining problems

Jeffrey Robinson JRobinson at pathology-associates.com
Mon Dec 14 13:10:09 CST 2015

Good Morning to all:  I am having staining problems with my PAX-5 predilute from CellMarque.  I was wondering if anyone else has experienced a dramatic dropoff in staining signal with this antibody after a few months.  I have now had this happen to me twice in the last 3 months.  It stains very nicely when the vial is first opened and seems to hold staining intensity for about 3 months and then drops off dramatically even though the expiration date is 2 years out.  I am using it on the Leica Bond.  I have been in contact with CellMarque about this problem but they tell me they have not had any complaints from any other labs.  I use many antibodies from CellMarque and have only seen this problem occur with one other antibody (ALK-1) about a year ago.  If you are experiencing this problem in your lab please contact Liz Beitman at CellMarque (lbeitman at cellmarque.com<mailto:lbeitman at cellmarque.com>) so that they can have all available information to further research this problem.  Thank you!
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

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