[Histonet] Cryostat sectioning of FF lymph node

[Martin, Erin]

Good morning!  My pathologists would like us to cut formalin fixed (not yet processed) tonsil tissue on a cryostat for DIF staining.  Has anyone done this?  I did a quick search that seemed to indicate that it was possible but that the architecture of the cells would be altered because of ice crystals and that it would be difficult to get the sections to stick.  If you have any advice I would greatly appreciate it!



Thanks,

Erin



Erin Martin, Histology Supervisor
UCSF  Dermatopathology and Oral Pathology Service
1701 Divisadero St, San Francisco, CA 94044
415-353-7248

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