[Histonet] curling of kidney sections in water path

[kbowden]

There is two things I do for kidney and liver tissue.

1. Add 5ml glycerol to the 100% Alcohol in the processor
2. After I face the block I soak the block in a dilution of liquid 
fabric softener. 40% fabric softener 60% water.

If the first thing doesn't fix the problem then I move on to the second 
solution.

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*Karen Bowden
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*University of CA, San Diego//*

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On 12/10/15 3:59 PM, mohamed abd el razik via Histonet wrote:
> dear all, i'm facing an issue that trouble us when we process tissue samples of different organs and cutting them; all organs are ok and spread fine and somoothly on ater path except for kidney sections (looks wrinkled and curled as if the praffin fram around it prevent it from spread!!!). i have tried using alcohol befor path and elevating the water temprature but no change in the sctions happened even after stay in the incubator).... any suggestions please
>
> thank you for your help
> Mohamed
> Cairo University
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