[Histonet] Histonet Digest, Vol 145, Issue 7

Anne Van Binsbergen annigyg at gmail.com
Mon Dec 7 20:51:22 CST 2015


Hi all
Anyone successfully using IDH1? Have been asked to source but am having a hard time finding a supplier. Anyone?
Ta
Anne

Sent from my iPhone

> On Dec 7, 2015, at 10:00 PM, histonet-request at lists.utsouthwestern.edu wrote:
> 
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> Today's Topics:
> 
>   1. Re: Histonet Digest, Vol 145,    Issue 6 Oct separating from
>      epidermis (Steve McClain)
>   2. RET mutant positive lung FFPE BLOCK (Clarence Owens)
>   3. Clear-rite Thresholds (Sands, Jenn)
>   4. New CPT Code 88350 (Abbott, Tanya)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Sun, 6 Dec 2015 20:29:22 +0000
> From: Steve McClain <SteveM at mcclainlab.com>
> To: "histonet at lists.utsouthwestern.edu"
>    <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Histonet Digest, Vol 145,    Issue 6 Oct
>    separating from epidermis
> Message-ID: <EA10108F-ED33-47EC-B363-9EA70B1668EB at mcclainlab.com>
> Content-Type: text/plain; charset="us-ascii"
> 
> 
> Skin is often difficult to cut. Not sure I have seen that artifact, but here is what I use when having difficulties.
> Gently blot the tissue/epidermis dry before immersing in liquid OCT/Neg50. Then freeze in cryostat. Avoid refrigerant spray.
> Sometimes we allow tissue to sit in oCT for 5 min and then freeze.
> If tissue was in 20% sucrose, we blot, then rinse in OCT for 5 min then replace/mount in new OCT and freeze.
> 
> Steve A. McClain, MD
> 
>> On Dec 6, 2015, at 13:10, "histonet-request at lists.utsouthwestern.edu" <histonet-request at lists.utsouthwestern.edu> wrote:
>> 
>> [Histonet] Oct separating from epidermis
> 
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Sun, 6 Dec 2015 17:31:33 -0500
> From: Clarence Owens <owensc500 at gmail.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] RET mutant positive lung FFPE BLOCK
> Message-ID: <8172D7CB-22B9-4C13-A16D-126EC4FFC1FB at gmail.com>
> Content-Type: text/plain;    charset=us-ascii
> 
> Hello all,
> 
> I am in search of a RET mutant positive lung FFPE block. If anyone has a block they can spare I would really appreciate your help. I am doing an assay validation and I am in need of this tissue. I'm willing to trade FFPE control material in return. If anyone can help I would really appreciate it. 
> 
> Regards,
> 
> Clarence Owens, HT (ASCP) QIHC
> 
> Sent from my iPhone
> 
> 
> ------------------------------
> 
> Message: 3
> Date: Mon, 7 Dec 2015 15:11:02 +0000
> From: "Sands, Jenn" <Jenn.Sands at EssentiaHealth.org>
> To: "histonet at lists.utsouthwestern.edu"
>    <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Clear-rite Thresholds
> Message-ID:
>    <389C7D597C19C94D9E6A51F6CAC225F3D386C4D5 at skimmer.medcampus.org>
> Content-Type: text/plain; charset="us-ascii"
> 
> Hi,
> 
> Has anyone determined a threshold for the Peloris II when using Clear-rite?
> 
> __________________________________
> Jenn Sands, CT(ASCP)CM HTL(ASCP)CM
> Anatomic Pathology Supervisor
> Essentia Health
> 32nd Avenue South
> Jenn.Sands at essentiahealth.org<mailto:Jenn.Sands at essentiahealth.org>
> 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 4
> Date: Mon, 7 Dec 2015 16:45:33 +0000
> From: "Abbott, Tanya" <TanyaAbbott at catholichealth.net>
> To: "histonet at lists.utsouthwestern.edu"
>    <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] New CPT Code 88350
> Message-ID:
>    <852F7D2C14FB464D80E182B15DB138AF884A3EC8 at CHIEX005.CHI.catholichealth.net>
>    
> Content-Type: text/plain; charset="us-ascii"
> 
> Has anyone determined how/when to use the new CPT code 88350 for Immunofluorescent studies? Is it like IHC? First IHC is 88342, the rest are 88341. So the  first IF is 88346, the rest ordered are 88350?
> Thanks in advance for your input!
> Tanya
> 
> Please note new email address: tabbott at pennstatehealth.psu.edu,<mailto:tabbott at pennstatehealth.psu.edu,> effective immediately
> 
> Tanya G. Abbott
> Pathology Manager - Histology/Cytology
> Penn State Health St. Joseph
> Phone:610-378-2635
> Fax: 610-898-5871
> 
> 
> 
> 
> 
> 
> ------------------------------
> 
> Subject: Digest Footer
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> ------------------------------
> 
> End of Histonet Digest, Vol 145, Issue 7
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