[Histonet] DIF on paraffin embedded tissue

[Morken, Timothy]

The response to a problem like this is that your lab has not validated such a procedure for diagnostic use so the results are completely unreliable with your current protocols. Sure, you can "try it" but a negative result would mean nothing at all. Or you might get massive background that also means nothing. To validate it would take days to weeks depending the number of stains, not to mention getting some the reagents necessary to get a good signal, and the many, many hours you would spend working it up. The ideal solution would be to find another lab that does have this validated and let them do it.

Your lab could validate it and have it ready for the next time, but it would probably be a rare procedure and many of the reagents would expire between uses.

Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
505 Parnassus Ave, Box 1656
Room S570
San Francisco, CA 94143

(415) 353-1266 (ph)
(415) 514-3403 (fax)
tim.morken at ucsfmedctr.org<mailto:tim.morken at ucsfmedctr.org>



Maryann Deathridge madeathridge at pastnashville.com <mailto:histonet%40lists.utsouthwestern.edu?Subject=Re%3A%20%5BHistonet%5D%20DIF%20on%20paraffin%20embedded%20tissue&In-Reply-To=%3C2ad258b08df04e578798657c1f439533%40pastnashville.com%3E>
Wed Nov 25 09:37:36 CST 2015
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We have a tissue sample that was processed and paraffin embedded.  We

URGENTLY need to recover the tissue and perform Immunofluorescence on the

sample.

 Does anyone have a procedure.   HELP



     madeathridge at pastnashville.com<http://lists.utsouthwestern.edu/mailman/listinfo/histonet>