[Histonet] rodent bubble brains

[Baker, Michael]

Hello, my simple mind is thinking: can you scan before extracting the brain? or,  can you extract the brain under water?  

Michael Baker, M.D. (CCHMC Pathology)

> Message: 11
> Date: Wed, 26 Aug 2015 16:10:21 +0100
> From: Ekaterina Vinnik
> Subject: rodent brain: how to avoid air bubbles in the ventricles after perfusion?
> 
> Dear all,
> I'm new to this list - and was really happy to find it. Does anybody have
> any advice on how to do rat brain extraction in such a way that the air
> would not get into the ventricles? We are doing MRI on the ex vivo rat
> brains, and the air which gets in causes nasty artifacts. I am looking for
> consistent way to avoid the bubbles, cause .
> The way we are extracting the brain now is to isolate the head, peel the
> skull from the top and from the sides, crush the nasal bone, expose the
> bulbs, remove dura, cut the nerves, lift the brain..  I was never concerned
> with this problem until I started doing MRI - and here it turns out to be
> really important.
> 
> I was wondering if I should just leave the dura in place during extraction,
> for example, would that work?
> 
> Thank you in advance!
> Katya
> 
> -- 
> Ekaterina Vinnik, MD, PhD
> Behavioural neuroscience lab,
> Champalimaud Neuroscience Programme,
> Lisbon,
> Portugal
> 
> +351918951392
> lulswinnik at gmail.com



----
Michael Baker, M.D.
CCHMC Pathology
T (513) 636-4261
F (513) 636-3924