[Histonet] Mouse brain and spinal cord

Michael LaFriniere Michael.LaFriniere at ccplab.com
Mon Aug 24 13:09:18 CDT 2015


In my experience Shouldn't hurt at 70-75 degrees c in oven over night 

Michael
Michael R. LaFriniere, HT (ASCP)
Executive Director


Capital Choice Pathology Laboratory
12041 Bournefield Way, Suite A . Silver Spring, MD 20904  
P: 240.471.3427 . F: 240.471.3401 . Cell 410-940-8844
michael.lafriniere at ccplab.com



-----Original Message-----
From: Kimberly Marshall via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Thursday, August 20, 2015 3:05 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Mouse brain and spinal cord


Howdy Histo folks. Quick question. I am working on a mouse brain and spinal cord research project. After cutting at 9 microns and staining routine H&E I had several wash. Today I am sectioning again, and thinking about putting in the oven over night before staining. Will this hurt the tissue?? I am new to animal Histo so learning as I go. I am doing LFB's on these slides too, so will extra dryer time cause problems with that stain? Thanks in advance for any info!!!


Kimberly Marshall H.T.(ASCP)

Histology/Lab Supervisor

Toll Free 1-800-426-2099

Fax 801-584-5104

PO Box 17580

Salt Lake City, Utah 84107

www.animalreferencepathology.com<http://www.animalreferencepathology.com/>



Advancing the art and science of veterinary medicine



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