[Histonet] Nails:

[Michael Ann Jones]

After grossing in, we soak the nail in Nair (dab of HOT water mixed to
runny paste) for a couple of hours to soften the nail.
We face into the block, soak the face of the block in fresh Nair solution
again for at least 30 minutes.
Bake sections in the oven for 1 hour at least, sometimes hand stain so
that slide is treated gently.
Tissue can still fall of sometimes - nature of the beast!
I guess they use Nair for Rhinoceros horns!
Good Luck :)

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones at metropath.com






On 8/19/15, 4:20 PM, "Jb via Histonet" <histonet at lists.utsouthwestern.edu>
wrote:

>Does anyone have any special tricks to keeping nail sections on the
>slides?  I am doing a PAS stain and the tissue keeps falling off.
>
>Using + Slides, cut on 1-2 microns, baking 1/2 hr. Air drying, and this
>nail still wants to fall off.
>
>Any suggestions. Please help,
>
>Craig
>
>Sent from my iPhone
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