[Histonet] Xylene Free Labs - One Final Question

[Rene J Buesa]

I always bought my mineral oil from Jim Coyle Associates but the brand was PENRECO and I am sure you can ask them any supplier near your lab.This is the cheapest way of buying mineral oil, otherwise (from a pharmaceutical company) it will be EXTRAORDINARILY expensive.René 


     On Friday, August 14, 2015 11:56 AM, Brendal Finlay via Histonet <histonet at lists.utsouthwestern.edu> wrote:
   

 
Thank you for the responses to my previous questions.  One final question:
 
What suppliers sell mineral oil by the gallon? I've seen it online at a variety of prices, but my regular vendors don't seem to sell it by the gallon. If you are a vendor and offer it by the gallon, you may send me a quote, but please do not add me to any mailing lists or send repetitive emails. We are not yet ready to purchase anything as this is the initial stages of consideration.
 
Thank you again!
 
Brendal Finlay, HT (ASCP) 

-----Original Message-----
From: "Rene J Buesa" <rjbuesa at yahoo.com>
To: "Brendal Finlay" <brendal.finlay at medicalcenterclinic.com>, histonet at lists.utsouthwestern.edu
Date: 08/13/2015 15:33
Subject: Re: [Histonet] Xylene Free Labs - Coverslipping and Frozen Section Questions

  1-After you oven dry your stained sections, you use the very same medium you always have used. I used Permount.
2- Given the very special constrains of FS (ready for diagnoses within 20 minutes of receiving the specimen) I used an aqueous mounting medium. After the diagnosis was made, I eliminated the aqueous mounting medium, washed the stained sections → oven dried → coverslip with Permount.
René


 
  On Thursday, August 13, 2015 2:20 PM, Brendal Finlay via Histonet <histonet at lists.utsouthwestern.edu> wrote:



For those labs who are xylene free I have two questions:
 
1. What mounting medium do you use when coverslipping oven dried slides?
2.. How are you running down and coverslipping frozen sections after staining them?
 
I'd like to make sure the stains are preserved over time and my experience with aqueous mounting mediums has been that they do not preserve stains well.
 
Thank you!
 
Brendal Finlay, HT (ASCP) 



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