[Histonet] Xylene Free Labs - Coverslipping and Frozen Section Questions

[Rene J Buesa]

1-After you oven dry your stained sections, you use the very same medium you always have used. I used Permount.2- Given the very special constrains of FS (ready for diagnoses within 20 minutes of receiving the specimen) I used an aqueous mounting medium. After the diagnosis was made, I eliminated the aqueous mounting medium, washed the stained sections → oven dried → coverslip with Permount.René 


     On Thursday, August 13, 2015 2:20 PM, Brendal Finlay via Histonet <histonet at lists.utsouthwestern.edu> wrote:
   

 
For those labs who are xylene free I have two questions:
 
1. What mounting medium do you use when coverslipping oven dried slides?
2. How are you running down and coverslipping frozen sections after staining them? 
 
I'd like to make sure the stains are preserved over time and my experience with aqueous mounting mediums has been that they do not preserve stains well. 
 
Thank you!
 
Brendal Finlay, HT (ASCP) 



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