[Histonet] Histonet Digest, Vol 141, Issue 8

Coleman, Billy bcoleman at wustl.edu
Mon Aug 10 12:43:41 CDT 2015


FYI  we have the blocks.
________________________________________
From: histonet-request at lists.utsouthwestern.edu <histonet-request at lists.utsouthwestern.edu>
Sent: Saturday, August 8, 2015 12:00 PM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 141, Issue 8

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Today's Topics:

   1. Re: Histonet Digest, Vol 141, Issue 7 topic 2 (Brent Adams)
   2. formalin limits for drain disposal (Terri  Braud)
   3. rodent heart tissue harvesting (Amy Lee)


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Message: 1
Date: Fri, 7 Aug 2015 17:29:22 +0000
From: Brent Adams <badams at acadianagastro.com>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Histonet Digest, Vol 141, Issue 7 topic 2
Message-ID:
        <CY1PR0301MB1642D3CB35496550021231C8B1730 at CY1PR0301MB1642.namprd03.prod.outlook.com>

Content-Type: text/plain; charset="Windows-1252"

I had the same problem with StatLab markers.  I would have issues when I let tissue set
in formalin bottles all night. I would skip the formalin steps on the processor and go straight
to 70% alcohol. This would almost completely erase the writing on the cassettes.

The product manager informed me The Statmark Pens are made with an aqueous ink
and if exposed to fingers prints or other solutions prior to being set by submerged in
100% Alcohol they will be erased or badly smudged.

I keep a container of 100% alcohol on my grossing bench and dip the
cassettes first before adding tissue from the containers. by time I add the
tissue and close the cassette lid the alcohol has evaporated and locks the ink
into the plastic cassette. If they are still slightly wet or you have alcohol on
your hands they will still smudge so be careful handling cassettes.

I have not had issues since going to this process.


Brent Adams ? HT, BS, LPN,

www.acadianagastro.com

Acadiana Gastroenterology Associates, LLC
439 Heymann Blvd
Lafayette, LA  70503

tel:  (337) 269-1126
fax:  (337) 269-1476

________________________________________
From: histonet-request at lists.utsouthwestern.edu <histonet-request at lists.utsouthwestern.edu>
Sent: Friday, August 7, 2015 12:00 PM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 141, Issue 7

Send Histonet mailing list submissions to
        histonet at lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
        http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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When replying, please edit your Subject line so it is more specific
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Today's Topics:

   1. Re: Clia requirements on specimens collected from satelite
      offices. (White, Marcia)
   2. Manual Cassette Labelling (Joanne Clark)
   3. Re: Manual Cassette Labelling (Ingles Claire)
   4. Re: Manual Cassette Labelling (Kienitz, Kari)
   5. Embedding Centers (Mitchell, Natacha A)
   6. Re: Manual Cassette Labelling (Boyd, Debbie M)
   7. FW: Hyde-Away (Bustamante, Lin)


----------------------------------------------------------------------

Message: 1
Date: Thu, 6 Aug 2015 17:01:25 +0000
From: "White, Marcia" <MWhite at mhs.net>
To: "'Nicole Tatum'" <nicole at dlcjax.com>,
        "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Clia requirements on specimens collected from
        satelite        offices.
Message-ID:
        <8B4B5BF3FCB6D24CBEAA63ACA5FDAD3B514931AE at MHSEXMB04.mhs.net>
Content-Type: text/plain; charset="us-ascii"

Where it was processed and diagnosis rendered

Marcia M White
Pathology Manager
Memorial Regional Hospital
954-265-5371-office
954-967-7627-fax
MWhite at mhs.net







-----Original Message-----
From: Nicole Tatum [mailto:nicole at dlcjax.com]
Sent: Thursday, August 06, 2015 12:05 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Clia requirements on specimens collected from satelite offices.


Histos,


I have a question. We are a Derm POL and we recently opened a satellite office. I have been putting the satellite address on the pathology requisitions but do the path reports need to have that address or the address of the main lab where they were processed.  Bacisly does CLIA want the address where the specimen was from, or the address where it was processed.


Thank You,

Nicole Tatum

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------------------------------

Message: 2
Date: Thu, 6 Aug 2015 20:27:48 +0000
From: Joanne Clark <jclark at pcnm.com>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Manual Cassette Labelling
Message-ID:
        <0494A7D4E8CC254EA2FB81464982E378D9ABD883 at S10MAILD001N3.SH10.lan>
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Hi Histoland,

We have a satellite lab that manually prepares their cassettes.  We have had significant problems with the markers coming off after tissue processing.  We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues.  Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you?

Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284

pcnm.com




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do not disclose its contents or take any action in reliance on the information it
contains.

------------------------------

Message: 3
Date: Thu, 6 Aug 2015 20:50:05 +0000
From: Ingles Claire <CIngles at uwhealth.org>
To: Joanne Clark <jclark at pcnm.com>,
        "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Manual Cassette Labelling
Message-ID:
        <CBBD2D0A1B3B5945AF0138A0BA79A79BA3669C at UWHC-MBX07.uwhis.hosp.wisc.edu>

Content-Type: text/plain; charset="us-ascii"

We use Secureline Marker II/superfrost Histoprep pens from Fisher. Their item # is 14-905-30 for black, 14-905-35 for red. We have never had a problem with them smearing or fading on the cassettes no matter what we subject them to.
Claire

________________________________________
From: Joanne Clark via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Thursday, August 06, 2015 3:27 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Manual Cassette Labelling

Hi Histoland,

We have a satellite lab that manually prepares their cassettes.  We have had significant problems with the markers coming off after tissue processing.  We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues.  Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you?

Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284

pcnm.com




Disclaimer: This electronic message may contain information that is proprietary,
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_______________________________________________
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------------------------------

Message: 4
Date: Thu, 6 Aug 2015 13:50:58 -0700
From: "Kienitz, Kari" <kkienitz at orclinic.com>
To: Joanne Clark <jclark at pcnm.com>,
        "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Manual Cassette Labelling
Message-ID:
        <41400FFE517878449D89114DD2526090196602D20F at tocmail1.tocad.orclinic.com>

Content-Type: text/plain; charset="us-ascii"

Epic Scientific Super Moist Markers by Creative Waste Solutions
503-657-5711

They will not let you down :)


Kari Kienitz HT, (ASCP)
Histology Laboratory
Gastroenterology-EAST
The Oregon Clinic
1111 NE 99th Ave
Portland, OR  97220
503.935.8311
kkienitz at orclinic.com




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________________________________________
From: Joanne Clark via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Thursday, August 06, 2015 1:27 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Manual Cassette Labelling

Hi Histoland,

We have a satellite lab that manually prepares their cassettes.  We have had significant problems with the markers coming off after tissue processing.  We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues.  Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you?

Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284

pcnm.com




Disclaimer: This electronic message may contain information that is proprietary,
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recipient of this message, please notify the sender immediately and delete the
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do not disclose its contents or take any action in reliance on the information it
contains.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 5
Date: Thu, 6 Aug 2015 21:03:06 +0000
From: "Mitchell, Natacha A" <Natacha.Mitchell at harrishealth.org>
To: "'histonet at lists.utsouthwestern.edu'"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Embedding Centers
Message-ID:
        <6441A38AD60C3543B5861E7E678D0ABFB2D5DA at hhmsg02.hchd.local>
Content-Type: text/plain; charset="us-ascii"

My fellow histonetters,

Thank you all so much for the prompt response. All the posts/emails were very helpful and I must say this site is a priceless source of knowledge, wisdom and resource!!!

Natacha Mitchell

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------------------------------

Message: 6
Date: Fri, 7 Aug 2015 11:35:19 +0000
From: "Boyd, Debbie M" <DKBoyd at chs.net>
To: Ingles Claire <CIngles at uwhealth.org>, Joanne Clark
        <jclark at pcnm.com>,      "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Manual Cassette Labelling
Message-ID:
        <7EAFE982E328304DA6CE2B677BB76246A9F3F858 at TN001WEXMBX014.US.chs.net>
Content-Type: text/plain; charset="us-ascii"

We use KP Marker Plus from Mercedes Medical, www.mercedesmedical.com or 1-800-331-2716.
Alcohol and Americlear resistant.  We do not use Xylene.

Debbie M. Boyd HT (ASCP) | Chief Histologist  | Southside Regional Medical Center | 200 Medical Park Blvd.  |  Petersburg, Va.  23805 | PH 804-765-5025 | FAX 804-765-6058

________________________________________
From: Ingles Claire via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Thursday, August 06, 2015 4:50 PM
To: Joanne Clark; histonet at lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] Manual Cassette Labelling

We use Secureline Marker II/superfrost Histoprep pens from Fisher. Their item # is 14-905-30 for black, 14-905-35 for red. We have never had a problem with them smearing or fading on the cassettes no matter what we subject them to.
Claire

________________________________________
From: Joanne Clark via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Thursday, August 06, 2015 3:27 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Manual Cassette Labelling

Hi Histoland,

We have a satellite lab that manually prepares their cassettes.  We have had significant problems with the markers coming off after tissue processing.  We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues.  Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you?

Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284

pcnm.com




Disclaimer: This electronic message may contain information that is proprietary,
confidential, or legally privileged or protected. It is intended only for the use
of the individual(s) and entity named in the message. If you are not an intended
recipient of this message, please notify the sender immediately and delete the
material from your computer. Do not deliver, distribute or copy this message and
do not disclose its contents or take any action in reliance on the information it
contains.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 7
Date: Fri, 7 Aug 2015 14:49:38 +0000
From: "Bustamante, Lin" <LBUSTAMANTE at cvm.tamu.edu>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] FW: Hyde-Away
Message-ID:
        <94B6DC15AAF2F046BF847D4C1CA9AAC901560C1ECF at CVMMB02.cvm.tamu.edu>
Content-Type: text/plain; charset="utf-8"

Can someone help her please?
Thank you very much.
Lin

Lin S. Bustamante, B.S., H.T.(ASCP)
VIBS Histology Laboratory Supervisor
College Of Veterinary Medicine
Texas A&M University
College Station, Texas 77843-4458
Phone: (979) 845-3177
Fax: (979) 458-3499


From: Coral Gonzalez [mailto:rayengonzalez at yahoo.com]
Sent: Friday, August 07, 2015 9:28 AM
To: Bustamante, Lin
Subject: Hyde-Away

Hello,

Please when you can, ask on Histonet about the ppm legal limit for draining formalin neutralized with Hyde Away.


Thank you,

Rayen Gonzalez B.S., HT (ASCP)

------------------------------

Subject: Digest Footer

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------------------------------

End of Histonet Digest, Vol 141, Issue 7
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------------------------------

Message: 2
Date: Fri, 7 Aug 2015 18:04:06 +0000
From: "Terri  Braud" <tbraud at holyredeemer.com>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Cc: "rayengonzalez at yahoo.com" <rayengonzalez at yahoo.com>
Subject: [Histonet] formalin limits for drain disposal
Message-ID:
        <48E053DDF6CE074DB6A7414BA05403F8025728 at HRHEX02-HOS.holyredeemer.local>

Content-Type: text/plain; charset="us-ascii"

The regulations vary from city to city and are managed at the municipal level.  It is usually set and managed by the city chemist or the head of the EPA (Environmental Protection Agency) for your area.  It can also vary when compared to the total volume sewage discharged from your facility.  Believe it or not, in some places, small amounts can be approved for drain disposal without any neutralization.  Make friends with your city EPA rep.  It can be an invaluable relationship, and usually they will appreciate your effort to be compliant.
Regards, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874



------------------------------

Message: 3
Date: Fri, 7 Aug 2015 15:46:44 -0700
From: Amy Lee <amylee053 at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] rodent heart tissue harvesting
Message-ID:
        <CAOcXOZ1VLnG1v_bfFy_DWgQw2X2nfysbWf7xJ_fmiL=hGEe-DA at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Hello histonet,

My coworker wants me to ask experts here the best way to use Potassium
Chloride during rodent heart harvesting. Could any one share your
procedure? We searched around on internet but not much help.

If there's any way other than Potassium Chloride to relax heart muscle,
please advice too.

Thanks,

Amy


------------------------------

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End of Histonet Digest, Vol 141, Issue 8
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