[Histonet] Handling paraffin
John Kiernan
jkiernan at uwo.ca
Mon Aug 3 22:46:58 CDT 2015
Gloves and "Paraffin hazards?"
1. Flammable. White candles are made of paraffin wax. We light them ungloved.
2. Hot when melted, but not hot enough to cause even a first degree burn from a small amount on the skin.
3. Liquid enters clothing and solidifies therein. This is a real annoyance, but the safety experts couldn't care less. Wax is removable by soap & water at >60C (thank you, René Buesa) or by dry-cleaning.
4. Solid wax has to be trimmed with a sharp knife to prepare for microtomy. Gloves thick enough to prevent a cut finger probably would impede this skilled task.
5. Every microtome has a dangerously sharp knife, less so in recent decades with short disposable blades. This is a hazard of microtomy, not of paraffin wax. It might be possible to cut, recover and mount sections while wearing very thin (surgical) rubber gloves, but why?
John Kiernan
London, Canada
= = =
On 03/08/15, "Johnson, Carole via Histonet" <histonet at lists.utsouthwestern.edu> wrote:
> This is kind of an odd question, but I was asked by a pathologist for any SOPs or references for the necessity of wearing gloves when embedding and working with paraffin. I am not aware of sources other than the MSDS for the different formulations. Does anyone require gloves to be worn during embedding, specifically related to paraffin hazards?
>
> Carole Johnson
> Carole Johnson, HT(ASCP)cm
> New Mexico Department of Agriculture
> Veterinary Diagnostic Services
> 505.383.9299
>
> To understand is to stand under, which is to look up, which is a good way to understand
>
>
>
> (http://lists.utsouthwestern.edu/mailman/listinfo/histonet)
>
>
More information about the Histonet
mailing list