[Histonet] Histonet Digest, Vol 141, Issue 1
Subash Govender
subash.govender at uct.ac.za
Mon Aug 3 15:37:59 CDT 2015
-------- Original Message --------
Subject: Histonet Digest, Vol 141, Issue 1
From: histonet-request at lists.utsouthwestern.edu
To: histonet at lists.utsouthwestern.edu
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Today's Topics:
1. Re: Ventana H.Pylori antibody (White, Marcia)
2. (no subject) (Teresa Harris)
3. P16 (kalkhenaizi .)
4. Re: Breast fixation (Cartun, Richard)
5. Re: Ventana H.Pylori antibody (Cates, Julia)
----------------------------------------------------------------------
Message: 1
Date: Fri, 31 Jul 2015 17:09:00 +0000
From: "White, Marcia" <MWhite at mhs.net>
To: "'Jeffrey Robinson'" <JRobinson at pathology-associates.com>, "Cates,
Julia" <Julia.Cates at AHSS.ORG>, "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Ventana H.Pylori antibody
Message-ID:
<8B4B5BF3FCB6D24CBEAA63ACA5FDAD3B51490B6F at MHSEXMB04.mhs.net>
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We also are having the same problem with the Ventana H.Pylori antibody on our Ultras, I have tried the CellMarque product in the past and as mentioned still not ideal. Will try the Biocare product and see if the results improve for us also.
Marcia M White
Pathology Manager
Memorial Regional Hospital
954-265-5371-office
954-967-7627-fax
MWhite at mhs.net
-----Original Message-----
From: Jeffrey Robinson [mailto:JRobinson at pathology-associates.com]
Sent: Thursday, July 30, 2015 1:06 PM
To: Cates, Julia; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana H.Pylori antibody
Hi Julia- I have had a lot of experience trying to get a clean H. pylori. I don't recall using the Ventana H. pylori but I was using the CellMarque H. pylori for years. It would often have a lot of background as you describe. I run Ventana Benchmarks (XT and Ultra) and a Leica Bond. The slides I would run on the Benchmarks were usually cleaner than those run on the Bond but still not as clean as I would like. I finally switched over to BioCare's H. pylori after many complaints from my pathologists. It has been much cleaner on all of my IHC instruments. Actually, the ones I run on the Ultra are the cleanest I have ever seen. The slides run on the Bond may still have a little background but it is still much cleaner than the ones I used to run with the CellMarque antibody. One of my pathologists who used to complain about the excessive background now calls it his "go to" stain and is very happy with how crisp it is. The organisms stand out very nicely. BioCare will send you a free sample if you want to try it.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.
-----Original Message-----
From: Cates, Julia via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, July 30, 2015 8:30 AM
To: histonet-bounces at lists.utsouthwestern.edu; histonet at lists.utsouthwestern.edu
Subject: [Histonet] Ventana H.Pylori antibody
Good day Histonet,
I have seen in the past conversations regarding Ventana's H.Pylori antibody and how dirty looking the stain can be. We have been using this antibody for several years now and have never really been happy with the quality. We have tried the recommended methods to "clean up" the stain and still we run into repeating the stain and/or complaints from the pathologists. We are considering using a different vendor but my concern is that my efforts will be a lateral move. Is anyone using a product that produces a clean stain or is this something inherent to this antibody? Are the pathologists happy with it and not just tolerating it?
Thank you for your suggestions,
Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Florida Hospital Waterman
(352) 253-3333 ext.4346 | Fax: (352) 253-3592
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------------------------------
Message: 2
Date: Fri, 31 Jul 2015 13:18:00 -0400
From: Teresa Harris <Teresajharris at msn.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] (no subject)
Message-ID: <BLU436-SMTP183E0307C16C401E7D32BEAA88A0 at phx.gbl>
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Yes
Sent from my iPhone
------------------------------
Message: 3
Date: Fri, 31 Jul 2015 22:14:43 +0300
From: "kalkhenaizi ." <alkhenaizik at gmail.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] P16
Message-ID:
<CAB-j_berv9PLkQQehe2bi5Bn7-Ky==2QwUWevBorEew4dX36xA at mail.gmail.com>
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Hello Histonetters,
Happy Friday!
I need p16 antibody but not sure which vendor provide reliable antibody.
I'd appreciate any recommendation.
I also need melanoma and prostate ca control blocks, I can exchange with
the once I have.
Thank you and have a nice weekend.
Kadhem
Kadhem Alkhenaizi, MS
HTL (ASCP) MB, QIHC
Lab Manager
ExpressMed Labs
------------------------------
Message: 4
Date: Fri, 31 Jul 2015 19:28:44 +0000
From: "Cartun, Richard" <Richard.Cartun at hhchealth.org>
To: "Heckford, Karen - SMMC-SF" <Karen.Heckford at DignityHealth.org>,
"histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Breast fixation
Message-ID:
<9215BD4B0BA1B44D962A71C758B68D2E6B01855A at HHCEXCHMB03.hhcsystem.org>
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There are several studies (one listed below) that have addressed the issue of fixation past the "recommended" 72 hour maximum. In my opinion, the science behind these fixation recommendations is "weak" at best. In my own experience, I have seen no problem whatsoever going well past 72 hours of fixation. The majority of breast cases will show internal positive controls for ER and PR to validate that the tissue is satisfactory for testing. Remember, working with your pathologist(s) you can perform your own validation on-site for extended fixation time. The real culprit for poor ER, PR, and HER2 IHC results is "inadequate" fixation.
Reference: Tong LC, Nelson N, Tsourigiannis J, et al.: The effect of prolonged fixation on the IHC evaluation of ER, PR, and HER2 expression in invasive breast cancer: A prospective study. Am J Surg Pathol 2011;35:545-552.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 972-1596
(860) 545-2204 Fax
-----Original Message-----
From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, July 29, 2015 11:02 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Breast fixation
Good Morning,
I have a question about breast fixation. I am in a little bit of a pickle with the 6-72 hour rule for the fixation on breast tissue. Friday I am getting 2 breast cases in the afternoon and both will not have the required minimum 6 hour formalin fixation for my per diem to cut early Saturday morning. He will not be able to make it in again until Monday night. The tissue will be about 3-4 hours (this includes time on the processor) over the 72 hour maximum. Does anyone have any suggestions on what can be done? We are a one person show here.
Thanks,
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckford at dignityhealth.org
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------------------------------
Message: 5
Date: Fri, 31 Jul 2015 19:56:27 +0000
From: "Cates, Julia" <Julia.Cates at AHSS.ORG>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>,
"histonet-bounces at lists.utsouthwestern.edu"
<histonet-bounces at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Ventana H.Pylori antibody
Message-ID:
<BN3PR0101MB11876937285CA1B257E62B44928A0 at BN3PR0101MB1187.prod.exchangelabs.com>
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Wow! Thank you all so much for your responses. They have been very helpful; it's so great to have a community of professionals that are so willing to give of their knowledge.
Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Florida Hospital Waterman
(352) 253-3333 ext.4346 | Fax: (352) 253-3592
Confidentiality Statement: This email message, including any attachments, is for the sole?use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email.
Message: 1
Date: Thu, 30 Jul 2015 17:06:04 +0000
From: Jeffrey Robinson <JRobinson at pathology-associates.com>
To: "Cates, Julia" <Julia.Cates at AHSS.ORG>,
"histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Ventana H.Pylori antibody
Message-ID:
<204A03EB5A7F0A4BB1EEDD52A963829C16D9EB26 at PAEXCH1.PathologyAssociates.local>
Content-Type: text/plain; charset="us-ascii"
Hi Julia- I have had a lot of experience trying to get a clean H. pylori. I don't recall using the Ventana H. pylori but I was using the CellMarque H. pylori for years. It would often have a lot of background as you describe. I run Ventana Benchmarks (XT and Ultra) and a Leica Bond. The slides I would run on the Benchmarks were usually cleaner than those run on the Bond but still not as clean as I would like. I finally switched over to BioCare's H. pylori after many complaints from my pathologists. It has been much cleaner on all of my IHC instruments. Actually, the ones I run on the Ultra are the cleanest I have ever seen. The slides run on the Bond may still have a little background but it is still much cleaner than the ones I used to run with the CellMarque antibody. One of my pathologists who used to complain about the excessive background now calls it his "go to" stain and is very happy with how crisp it is. The organisms stand out very nicely. BioCare will send you a free sample if you want to try it.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.
-----Original Message-----
From: Cates, Julia via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, July 30, 2015 8:30 AM
To: histonet-bounces at lists.utsouthwestern.edu; histonet at lists.utsouthwestern.edu
Subject: [Histonet] Ventana H.Pylori antibody
Good day Histonet,
I have seen in the past conversations regarding Ventana's H.Pylori antibody and how dirty looking the stain can be. We have been using this antibody for several years now and have never really been happy with the quality. We have tried the recommended methods to "clean up" the stain and still we run into repeating the stain and/or complaints from the pathologists. We are considering using a different vendor but my concern is that my efforts will be a lateral move. Is anyone using a product that produces a clean stain or is this something inherent to this antibody? Are the pathologists happy with it and not just tolerating it?
Thank you for your suggestions,
Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Florida Hospital Waterman
(352) 253-3333 ext.4346 | Fax: (352) 253-3592
Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email.
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End of Histonet Digest, Vol 141, Issue 1
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