[Histonet] IHC and oven temperature

Thu Apr 30 14:56:02 CDT 2015

What about HIER at 95-98ºC? "Everybody" uses it to "enhance" epitopes detection.Is there not an intrinsic contradiction here?
René J.  

     On Thursday, April 30, 2015 3:32 PM, Tony Henwood (SCHN) <tony.henwood <@t> health.nsw.gov.au> wrote:

Yes,

I read the Dako IPX educational guides (5th ed) and on page 32:
"No processes should raise tissue temperature to higher than 60oC as this will cause severe loss of antigenicity that may not be recoverable"
Unfortunately there is no evidence given or cited that validates this statement. Even though this could be right (and there are several papers that have looked at this), this statement is scientifically weak and we should not cite this as truth.

Now I do recommend the Dako reference series to my students, and I have contributed to one of these texts myself (Microscopic control of routine H&E - know your histology) but I request my students to continue to question what they read and confirm the scientific validity of the information.

Regards,
Tony

________________________________________
From: Joelle Weaver [joelleweaver <@t> hotmail.com]
Sent: Saturday, 25 April 2015 5:51 AM
To: Tony Henwood (SCHN); WILLIAM DESALVO; Preiszner, Johanna
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC and oven temperature

I remember reading that the preffered temperature was about 60 degrees Celsius. I think that this was in the Dako education guides if I'm not mistaken. If that is the case, the citation for the source is probably in that resource available as pdf from their website.

Joelle Weaver MAOM, HTL (ASCP) QIHC

> From: tony.henwood <@t> health.nsw.gov.au
> To: wdesalvo.cac <@t> outlook.com; PREISZNE <@t> mail.etsu.edu
> Date: Fri, 24 Apr 2015 09:43:59 +0000
> Subject: RE: [Histonet] IHC and oven temperature
> CC: histonet <@t> lists.utsouthwestern.edu
>
> Hi temp drying shown to be a bad idea:
>
> Henwood, A., (2005) “Effect of Slide Drying at 80°C on Immunohistochemistry” J Histotechnol 28(1):45-46.
>
> Abstract
>
> Prolonged high temperature dry heating has been found to be deleterious to the immunohistochemical demonstration of several antigens in formalin-fixed, paraffin- embedded sections. Paraffin sections were dried at 80°C for 7 h and their immunoreactivity was compared with mirror sections dried for 1 h at 60°C. NCL-5D3, CMV, S100, HMB45, and CEA were quite labile to dry overheating whereas AElAE3, HBsAg, HBcAg, HSVII, EMA, chromogranin, and NSE were found to be quite resistant. It is recommended that coated slides (poly-L-lysine or aminopropyltriethoxysilane) and low-temperature drying (<60°C) be routinely used for irnmunohistochemistry.
>
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo.cac <@t> outlook.com]
> Sent: Tuesday, 21 April 2015 1:56 AM
> To: Preiszner, Johanna
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] IHC and oven temperature
>
> Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes
>
> Sent from my iPhone
>
> > On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna <PREISZNE <@t> mail.etsu.edu> wrote:
> >
> > Hi Netters,
> >
> > is there something wrong with this logic:
> >
> > "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven."
> >
> > Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer...
> >
> > Thanks!
> >
> > Hanna Preiszner
> > ETSU/QCOM
> >
> >
> > _______________________________________________
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> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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     On Thursday, April 30, 2015 3:32 PM, Tony Henwood (SCHN) <tony.henwood <@t> health.nsw.gov.au> wrote:

Yes,