[Histonet] RE: Centrifuge for cytospins

Tue Apr 28 13:31:18 CDT 2015

In all of my experiences with cytology preparations, the first step would be to centrifuge the specimen.  This concentrates the cells for the next step; cytocentrifuge, which is used for the presentation of the cells for evaluation.  Here is an excerpt from our procedure:

3.      Label a 50 ml centrifuge tube with the cytology accession label.
4.      Mix the specimen using a vortex mixer for 10-30 seconds.
5.      Pour contents of specimen from the original container into the 50 ml centrifuge tube and cap the tube.
6.      Centrifuge the specimen at 600g for 10 minutes (program 1) or 1200g for 5 minutes (program 2).
7.      Pour off the supernatant and re-suspend the sediment in 0.5 to 1ml of Cytospin (green) fluid, depending on the cell button size.

Evaluating the number of drops you might need for your cytocentrifuge can be performed by taking a crop from the sediment, placing it on a slide, placing a 24x50mm cover glass on the specimen and then observing the cellularity of the specimen.  I have document that illustrates this but I don't think the list serve will allow attachments.  You can email me privately for this document.

Joe W. Walker, Jr. MS, SCT(ASCP)CM
Manager of Anatomical Pathology, Microbiology and Reference
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P: 802.747.1790  F: 802.747.6525
Email joewalker <@t> rrmc.org    www.rrmc.org

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Tuesday, April 28, 2015 12:10 PM
To: Piche, Jessica; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Centrifuge for cytospins

We make our BAL cytospins directly--no centrifuging first.

Brian

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Piche, Jessica
Sent: Tuesday, April 28, 2015 8:58 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Centrifuge for cytospins

Hi Everyone,

Just asking a quick question for our Cyto department. Are BAL slides centrifuged and then made in to cytospin slides or do you just make the cytospin slides with no centrifuging?

Thank you,

Jessica Piche, HT(ASCP)

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