[Histonet] Fixation of frozen section

[Keyser Gerald T]

We get good results with 95%. Unless prepping sample for Electron Microscopy, cell membrane preservation doesn't seem to be worth worrying about. It can't be resolved with a light microscope.  A light microscope using a violet monochromatic light source should have the theoretical limits of resolution of  approx. 200nm. Cell membranes... what do they run...  approx. 4nm? 

Gerry 



-----Original Message-----
From: Jamal [mailto:j.rowaihi <@t> alborglaboratories.com] 
Sent: Tuesday, April 28, 2015 12:07 PM
To: 'Manahil'; Keyser Gerald T
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Fixation of frozen section

You can't use full concentrate alcohol on such specimen, otherwise you will destroy the cells membrane.
For me I am using 80% Ethanol.

Best Regards,


Jamal Rowaihi	Anatomic Pathology Supervisor       | Al Borg Medical
Laboratories

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Manahil
Sent: Tuesday, April 28, 2015 7:23 PM
To: Keyser Gerald T
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Fixation of frozen section

What about the Absolute Methanol?

Sent from my iPhone

> On Apr 28, 2015, at 8:16 PM, Keyser Gerald T <GKeyser <@t> uwhealth.org> wrote:
> 
> Our lab uses 95% Ethanol. It takes longer to fix the tissue than formalin.
But, it's effective and far less toxic than formalin. 
> 
> Gerry
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Manahil
> Sent: Tuesday, April 28, 2015 11:11 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Fixation of frozen section
> 
> Hi histonet,
> 
> Would like to query about which fixative do you prefer for fresh 
> frozen
section slide before H&E staining?
> Thanks for your advices
> Manahil
> HTL/ ASCP
> 
> 
> Sent from my iPhone
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