[Histonet] RE: Nuclear "Artifact"

[Joelle Weaver]

I vote for "wicking" from the papers or other absorbant material. It does not seem like it would affect only sporadic biopsies all processed on the same processor at the same wax temperature. Other artifacts ( sectioning) are histology technique. Other nuclear issues could be staining, but this one doesn't fit that mold. Let us know how the experiment turns out. 


Joelle Weaver MAOM, HTL (ASCP) QIHC

        
  

 
> From: tony.henwood <@t> health.nsw.gov.au
> To: suetp918 <@t> comcast.net; Royl1 <@t> LabCorp.com
> Date: Fri, 24 Apr 2015 09:12:07 +0000
> CC: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: Nuclear "Artifact"
> 
> This seems like a classic case of drying of biopsies prior to fixation. This can occur if biopsies are placed on absorbent paper (or on disinfecting alcohol swabs, heaven forbid).
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Sue [suetp918 <@t> comcast.net]
> Sent: Friday, 24 April 2015 10:51 AM
> To: Lisa Roy
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Nuclear "Artifact"
> 
> Hi All
> 
> So I have been seeing the same issue as I stated in past e-mails. I did one test and fixed a colon biopsy in formaldehyde and left it in overnight and processed next day. I was hoping that I could reproduce the artifact. The tissue was beautiful. At my pathologists requests we changed the paraffin temperature tonight is the first night. I do not think this i the issue. We are going to transfer our biopsies to another tissue processor just for test. I brought up in the past that i think the issue starts prior to the histo lab, my pathologist tended to disagree, but I think he is chaining his mind since my one common detonator is a PA. I do not think that she wets her blue wrap paper enough and the tissue sits on the paper dry she also fold the paper so tight that it is possible for the small biopsies to get stuck in a fold. My pathologists actually came in and said he thought I may be right. Wow. That is my next test. We are requiring our staff to do so much work that they tend to rush and as I have stated in the past grossing sets the tone for every step the nistotech is responsible for and if it is not prepared correctly in the gross lab the histologist cannot fix it. An old adage we are not magicians.
> 
> Sue Paturzo
> TJUH
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