[Histonet] RE: Decalcification using EDTA

Mack, Sarah Sarah_Mack <@t> urmc.rochester.edu
Fri Apr 24 12:09:15 CDT 2015


It is optional to decal at 4 degrees Celsius.  We decal most of our bone samples in EDTA at room temperature.

Sarah Mack
University of Rochester Medical Center
Center for Musculoskeletal Research
Histology, Biochemistry, and Molecular Imaging Core
601 Elmwood Avenue
Box 665
Rochester, NY 14642
(585)-273-3901
________________________________________
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Sent: Friday, April 24, 2015 1:03 PM
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Subject: Histonet Digest, Vol 137, Issue 31

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Today's Topics:

   1. Decalcification using EDTA (Rooki Parak)
   2. Leica perfusion 1 or 2 systems (Caroline Miller)
   3. RE: Histonet Digest, Vol 137, Issue 30 (Mayer,Toysha N)
   4. RE:Histology in higher education (Mayer,Toysha N)
   5. RE: NY State Histology License (Anne Murvosh)
   6. RE: Histonet Digest, Vol 137, Issue 30 (Chapman, Cherie J.)


----------------------------------------------------------------------

Message: 1
Date: Fri, 24 Apr 2015 13:05:33 +0200
From: Rooki Parak <rooki.parak <@t> gmail.com>
Subject: [Histonet] Decalcification using EDTA
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <CAP6FbXhgd8tBzxHcxrkah=EeJNaqyqibr0JD7DB3WNnWB6FNcw <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Hello Histonetters

Is it imperative OR optional to maintain samples at below 4 degrees celcius
when using EDTA to decalcify bone samples

Thank You


------------------------------

Message: 2
Date: Fri, 24 Apr 2015 07:38:00 -0700
From: Caroline Miller <mills <@t> 3scan.com>
Subject: [Histonet] Leica perfusion 1 or 2 systems
To: "Histonet <@t> Lists. Edu" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <CAFTHRQMaSdNVZiEP6OPb1nnCbOvNr2sRgtDcZx3xtW=KcVCzfg <@t> mail.gmail.com>
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Happy Friday Histonet!

Does anyone have a perfusion one or two system from Leica that they can
tell me how it works, or even better take a little movie?

We are testing out different mechanisms of perfusion but do not want to buy
one until we know if it is going to work for us! I asked my rep for a demo
too.

thanks all!

mills

--
Caroline Miller
Director of Histology
3Scan.com
415 2187297


------------------------------

Message: 3
Date: Fri, 24 Apr 2015 14:45:05 +0000
From: "Mayer,Toysha N" <TNMayer <@t> mdanderson.org>
Subject: [Histonet] RE: Histonet Digest, Vol 137, Issue 30
To: "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <47E9B2C01DDDD94881EACD2DC44EBC881D56425D <@t> D1PWPEXMBX05.mdanderson.edu>
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Gail,
The regulation is from CLIA '88.  They list the requirements for that, and I have never heard of a grandfather clause.  I could be wrong about though.
NY state did not license HTL.  I had a student who wanted to move there, and did not because he was going to have an HTL, not HT.  He would only have been able to work clinical if he took both exams (HT and HTL).  I even contacted the state society for clarification.  Hopefully it has changed.
I believe it is because they do not have any HTL programs in the state, so it was not included in the licensure bill.

Sincerely,

Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
UT M.D. Anderson Cancer Center
713.563-3481


Message: 8
Date: Thu, 23 Apr 2015 18:34:13 +0000
From: Joelle Weaver <joelleweaver <@t> hotmail.com>
Subject: RE: [Histonet] NY State Histology License
To: Gail Marcella <gmarcella <@t> nj-urology.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT149-W8666703AAC97B9793FDBC9D8ED0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

Yes, CLIA stipulation. I think that there may be a grandfather clause, but not sure of the time frame. You could check the regulation on that.


Joelle Weaver MAOM, HTL (ASCP) QIHC





> From: gmarcella <@t> nj-urology.com
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 21 Apr 2015 11:47:58 -0400
> Subject: [Histonet] NY State Histology License
>
> Hi  - I've been a Histotech for 20+ years and got my Clinical Laboratory License in NY State when they required getting it. I don't have an Associates or Bachelor's degree but a Pathologist signed off for me. I have my HTASCP. I was told when I went for an interview in NY State that I couldn't gross small specimens or do IHC without an Associates  or Bachelor degree in biology. I was not aware of these restrictions. I don't see anything on the NYS website.  I was wondering if anyone else heard of this? Thanks - Gail
> _______________________________________________
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------------------------------

Message: 4
Date: Fri, 24 Apr 2015 15:06:13 +0000
From: "Mayer,Toysha N" <TNMayer <@t> mdanderson.org>
Subject: [Histonet] RE:Histology in higher education
To: "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <47E9B2C01DDDD94881EACD2DC44EBC881D56436C <@t> D1PWPEXMBX05.mdanderson.edu>
Content-Type: text/plain; charset="utf-8"

Thanks for the info.  I just signed up.  The whole program sounds like a great idea.  A few years ago I spoke at my son's middle school about histology and what the job entails.  It is challenging to figure out how to reach kids at their level.
I may talk about the program in my class next fall to introduce them to outreach in the science fields.

Great info.

Sincerely,

Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
UT M.D. Anderson Cancer Center
713.563-3481




Message: 11
Date: Wed, 22 Apr 2015 16:24:28 +0000
From: "Piche, Jessica" <jpiche <@t> wtbyhosp.org>
Subject: RE: [Histonet] histology in higher education
To: "'Grantham, Andrea L - (algranth)'" <algranth <@t> email.arizona.edu>,
        "\"\""  <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <631955447A364B45B9458D2905635110D8BF681F <@t> WIN08-MBX-01.wtbyhosp.org>
Content-Type: text/plain; charset="us-ascii"

Thank you for sharing this information Andi. I'd like to do something like this and I'm going to send this on to my daughters science teachers at school. I think it's a great idea. It always amazed me all the different jobs in hospitals alone that are available for kids and adults alike and no one knows they exist. Especially histology. Looking forward to passing this on!
Thanks again,
Jessica Piche, HT(ASCP)
Waterbury Hospital
CT

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth)
Sent: Wednesday, April 22, 2015 12:01 PM
To: ""
Subject: RE: [Histonet] histology in higher education

Bonnie, and anybody who wants to do this:
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.prescientist.org&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=9GrlE0JWzYNdPAj93-f_VHrRwq-gg31O3ro05gxX1ys&s=EufgUSX4U6zECw6kT_EQ9K2y_dxbt-V5A8eIEUojmsI&e=


________________________________________
From: Whitaker, Bonnie [Bonnie.Whitaker <@t> osumc.edu]
Sent: Wednesday, April 22, 2015 8:45 AM
To: Grantham, Andrea L - (algranth); ""
Subject: RE: [Histonet] histology in higher education

Andi,

Would you be willing to share the information on how to volunteer with this program?

Thanks,
Bonnie

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth)
Sent: Wednesday, April 22, 2015 11:40 AM
To: ""
Subject: RE: [Histonet] histology in higher education

For a few years I've been involved in a program called "letters to a pre-scientist". The idea is to reach middle schoolers as they are being introduced to the sciences. They have pretty high goals at this time, they want to be doctors and astronauts and engineers but they are just starting to learn about these things.
You become a pen pal/mentor of sorts and write letters to a child and they will write back to you. Last year I was writing to a boy in the Chicago area and this year it was a girl in LA. I always write about what I do and how important it is and include pictures of things like brain cells, muscle, fungus, bacteria and pictures of my lab. I always pick up a copy of the NSH coloring book and send it to them and tell them what they need to study to be a histotech and other than a hospital, where they can find a job. Of course we also tell them about other things like our families, pets, vacations, etc. at the same time.

It's just a small thing but it plants a seed.

Andi G.

------------------------------

Message: 5
Date: Fri, 24 Apr 2015 16:03:10 +0000
From: Anne Murvosh <amurvosh <@t> advancederm.net>
Subject: [Histonet] RE: NY State Histology License
To: Gail Marcella <gmarcella <@t> nj-urology.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <22BDD9AABC13E24E95D1CF064B75C4B7A82650 <@t> Exchange.Advancederm.net>
Content-Type: text/plain; charset="us-ascii"

There is a grandfather clause if you were a histotech by april 24.1995.  I asked ClIA recently when they came to inspect me, as I fell into this category but didn't know if you had to have the licence by then or just have been performing high complexity test at that time (my licence was later than that).  The CLIA person couldn't decipher their own rules and was going to ask her supervisor.  I never heard back.  As for IHC any HT in our state can do it and CAP never brought it up when I worked at the hospital.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gail Marcella
Sent: Tuesday, April 21, 2015 8:48 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] NY State Histology License

Hi  - I've been a Histotech for 20+ years and got my Clinical Laboratory License in NY State when they required getting it. I don't have an Associates or Bachelor's degree but a Pathologist signed off for me. I have my HTASCP. I was told when I went for an interview in NY State that I couldn't gross small specimens or do IHC without an Associates  or Bachelor degree in biology. I was not aware of these restrictions. I don't see anything on the NYS website.  I was wondering if anyone else heard of this? Thanks - Gail
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------------------------------

Message: 6
Date: Fri, 24 Apr 2015 16:28:54 +0000
From: "Chapman, Cherie J." <chapmanc <@t> health.missouri.edu>
Subject: [Histonet] RE: Histonet Digest, Vol 137, Issue 30
To: "Mayer,Toysha N" <TNMayer <@t> mdanderson.org>,
        "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <E65BED72A77E37438817B2A1F4B4E6AD645E6013 <@t> UM-MBX-T01.um.umsystem.edu>
Content-Type: text/plain; charset="us-ascii"

FYI-
This is what we have listed in our CLIA Testing Personnel Qualifications-High Complexity Testing-
Minimum requirements:
Earned Associates' degree in laboratory science or medical laboratory technology.  (Equivalency: 60 semester hours with adequate course break-down and training experience).
Or
Prior to April 24, 1995, have graduated from a medical laboratory school that has been approved or have completed a 50-week military training course.
High school graduates with appropriate training who are performing high complexity testing on or before April 25, 1995, will continue to qualify. This could be referred to as the grandfather clause.

I hope this helps.

Cherie Chapman
BS, HT, HTL (ASCP)

This
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N
Sent: Friday, April 24, 2015 9:45 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: Histonet Digest, Vol 137, Issue 30

Gail,
The regulation is from CLIA '88.  They list the requirements for that, and I have never heard of a grandfather clause.  I could be wrong about though.
NY state did not license HTL.  I had a student who wanted to move there, and did not because he was going to have an HTL, not HT.  He would only have been able to work clinical if he took both exams (HT and HTL).  I even contacted the state society for clarification.  Hopefully it has changed.
I believe it is because they do not have any HTL programs in the state, so it was not included in the licensure bill.

Sincerely,

Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center
713.563-3481


Message: 8
Date: Thu, 23 Apr 2015 18:34:13 +0000
From: Joelle Weaver <joelleweaver <@t> hotmail.com>
Subject: RE: [Histonet] NY State Histology License
To: Gail Marcella <gmarcella <@t> nj-urology.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT149-W8666703AAC97B9793FDBC9D8ED0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

Yes, CLIA stipulation. I think that there may be a grandfather clause, but not sure of the time frame. You could check the regulation on that.


Joelle Weaver MAOM, HTL (ASCP) QIHC





> From: gmarcella <@t> nj-urology.com
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 21 Apr 2015 11:47:58 -0400
> Subject: [Histonet] NY State Histology License
>
> Hi  - I've been a Histotech for 20+ years and got my Clinical
> Laboratory License in NY State when they required getting it. I don't
> have an Associates or Bachelor's degree but a Pathologist signed off
> for me. I have my HTASCP. I was told when I went for an interview in
> NY State that I couldn't gross small specimens or do IHC without an
> Associates  or Bachelor degree in biology. I was not aware of these
> restrictions. I don't see anything on the NYS website.  I was
> wondering if anyone else heard of this? Thanks - Gail
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=9GrlE0JWzYNdPAj93-f_VHrRwq-gg31O3ro05gxX1ys&s=5rH0Te-4CBbuQ0YFVCQg1otzKJYnWJX4GncnnPfr7AQ&e=



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