[Histonet] RE: Nuclear "Artifact"

[Podawiltz, Thomas]

I had this issue a couple of years back. Found out that there was a delay of over an hour from the time the specimen was collected to the time it was being put into fixative. 

Tom 


Tom Podawiltz HT (ASCP)
AP  Section Head 
LRGHealthcare
 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Roy, Lisa
Sent: Tuesday, April 21, 2015 1:41 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Nuclear "Artifact"

Hi HistoNetters:
I have run into quite a problem.  My lab currently processes all tissue types from 3 different sites.  Recently, we have been getting complaints from one of the sites that the biopsies have a nuclear "artifact".  It is described as "washed out" or poor to no nuclear detail.  Pictures have been uploaded (Nuclear "Artifact").  The Medical Director at said site is convinced that a processor error is occurring.  Our site is not seeing this on any of our slides.  Biopsies from all three sites are processed and embed together.  We have done all trouble shooting that we can think of.  Leica service has come to inspect our Peloris processor and all areas checked out as functioning properly.  The problem is not consistent daily.  Seems to be worst toward the end of the week.
We have been running the same processing protocol, staining protocol and cutting protocols for years now.  This problem has just developed over the last 2 months.  Any ideas, no matter how far-fetched, would be greatly appreciated at this point.

Lisa Roy, HT(ASCP)
Histology Supervisor
LabCorp at St. Vincent Hospital
123 Summer St
Worcester, MA
(508)363-9420

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