[Histonet] ORO tissue falling off

[Bryan Llewellyn]

There are a couple of old techniques you might try.

1. Smear some Mayer's egg albumen on a slide as you would normally do, 
i.e. very thinly, then hold it in a flame until it smokes. Allow to cool 
and pick up the section. Allow to drain well.

2. Same as 1, but place the slide with the picked up tissue in a coplin 
jar with a couple of mL concentrated formalin in the bottom for a hour 
or so to fix the albumen.

3. Same as 1 and 2, but use 1% gelatin instead of Mayer's egg albumen.

4. If all else fails, stain free floating sections of about 15 microns 
and pick up on a slide immediately before coverslipping. This was the 
way fat stains were done years ago before the cryostat.

Bryan Llewellyn



Jo-Ann Bader, Ms. wrote:
> We are having difficulty with a particulate set of very, very fatty mouse livers.  The normal livers from this set stay on the slides the fatty livers fall off.  We have used different types of charged slides and we have even tried to drench the charged slides in Stay-On, dry them and then put the frozen tissues on (despirate times call for despirate measures).  No luck  Does anyone have any other ideas.  Help Help
>
> Jo-Ann  Bader
> Histology Coordinator
> Goodman Cancer Research Center
> 1600 Pine Ave. W,
> Room 312
> Montreal Quebec, H3A 1A3
> Email: jo-ann.bader <@t> mcgill.ca<mailto:jo-ann.bader <@t> mcgill.ca>
> Office Tel:  514-398-5647
> Lab:  Tel:  514-398-8270
>
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