[Histonet] Re: Paraffin embedding of whole mouse brain

[Samala, Ramakrishna]

Hello Histonet!

Thank you all for your time and wonderful suggestions. This is the very
first time, I have to work with paraffin embedded brain tissues.

Based on the suggestions from Histonet colleagues, I have decided to
obtain 200 um thick brain sections for processing and embedding. Since I
have about 30 mouse brains, I have to be very careful in labeling all
cassettes. So problem solved!!!!!

Again thank you all

Have a very good week

Sincerely

Ramakrishna Samala, Ph.D.
Senior Research Associate
Amarillo Research Building, School of Pharmacy, Texas Tech University
Health Sciences Center
1406 S. Coulter
Amarillo, TX 79106


The outcome of any serious research can only be to make two questions grow
where only one grew before ­Thorstein Veblen








On 4/13/15, 9:30 AM, "Scouten, Charles"
<Charles.Scouten <@t> LeicaBiosystems.com> wrote:

>Are you really embedding, or just encasing?  I have heard that paraffin
>cannot penetrate that deeply, that small sections are necessary for the
>paraffin to infiltrate the entire piece.  Is this not true?
>
>Why not use frozen sections?  You can encase the brain in premade gelatin
>molds see this link:
>http://cp.mcafee.com/d/FZsS92gscyhJ5xdUQsILIfFCXCQQm4n73hOqenTzqqb2bwWZPhO
>Orjhohssd79EVvd79J6ZT3hPtdBAsrzGKBoOEjxLkRnjZ1kVJAWmXQ6PsVJAWmXQ6PrOba11dZ
>_HYyOCYUzvHTbFIFIYC---UCZORQX8FGEEKsG7DR8OJMddECSjt-hojuv78I9CzATsS02f-lJ8
>iHgJivGQVv8_jZzoDAfrzmBFv2TVelad-nFZFOH2k2e8vfp7QNqdk6XjM_VmQKgzIE5O1Zr5vy
>nH3YdS9X4_WtiRmV_057_aSA9lEmFfRqsKrsohudwIqid40mSeKKOwq8fl8uwxa14Qgltd41wD
>k_Ph0HlKDCy0x7pgdIcCYtznMVY5iQrl
>and section gelatin and all.  Every brain in the same plane of section.
>
>Do you use fixation perfusion, or just extract the soft brain?  Animal
>researchers routinely use perfusion for the better tissue quality.
>
>
>Cordially,
>
>Charles W. Scouten, Ph.D.
>Applications Specialist
>Leica Biosystems
>Charles.Scouten <@t> Leicabiosystems.com
>http://cp.mcafee.com/d/avndz9J5xdUQsILIfFCXCQQm4n73hOqenTzqqb2bwWZPhOOrjho
>hssd79EVvd79J6ZT3hPtdBAsrzGKBoOEjxLkRnjZ1kVJAWmXQ6PsVJAWmXQ6PrOba11dZ_HYyO
>CYUzvHTbFIFIYC---UCZORQX8FGEEKsG7DR8OJMddFCSjt-hojuv78I9CzATsS02lbgZKdjZ8s
>KrIjS9_QWBGJP-0af-lJ8iHgJivGQVsSUMyYr1oQAq80JItttB0QguGgZ12k29EwGWq831eF_C
>y1mHtfd412eOwropdIgvrKJ9Lwge
>Ph.  630 964 0501
>Cell  314 724 5920
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Samala,
>Ramakrishna
>Sent: Friday, April 10, 2015 9:00 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Paraffin embedding of whole mouse brain
>
>Hello Histonet!
>
>I would like to paraffin embed whole mouse brain to obtain coronal
>sections. I spoke with customer support teams of several companies, I
>could able to get 10 mm deep molds,  but the mouse brain is about 13 mm
>height. So, any help in this regard is much appreciated.
>
>Sincerely
>
>Ramakrishna Samala, Ph.D.
>Senior Research Associate
>Amarillo Research Building, School of Pharmacy, Texas Tech University
>Health Sciences Center
>1406 S. Coulter
>Amarillo, TX 79106
>
>The outcome of any serious research can only be to make two questions
>grow where only one grew before -Thorstein Veblen
>
>
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