[Histonet] RE: Paraffin embedding of whole mouse brain

[Anne Murvosh]

I have actually embedded specimens that the PA cut in too thick with the cassette upside down to form a dome, using a lot of paraffin.  I don't know if it would work with something so large.  You could try one of the deep cassettes I've seen in catalogs with the large mold, if regular cassette is still too small.  It's a little crazy, but an option if there are no others.  Anne

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Samala, Ramakrishna
Sent: Friday, April 10, 2015 7:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Paraffin embedding of whole mouse brain

Hello Histonet!

I would like to paraffin embed whole mouse brain to obtain coronal sections. I spoke with customer support teams of several companies, I could able to get 10 mm deep molds,  but the mouse brain is about 13 mm height. So, any help in this regard is much appreciated.

Sincerely

Ramakrishna Samala, Ph.D.
Senior Research Associate
Amarillo Research Building, School of Pharmacy, Texas Tech University Health Sciences Center
1406 S. Coulter
Amarillo, TX 79106

The outcome of any serious research can only be to make two questions grow where only one grew before -Thorstein Veblen


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