[Histonet] Positive Control for IF?

[Jim Burchette]

Paula, does your institution perform autopsies? If so, have the staff
notify you when there is a lupus nephritis case. Have them send you
fresh renal cortex. Cut up the tissue into small cubes and freeze for later
use as IF control. Run your battery of Ig's, compliment and light chains.
Most should have some reactivity.
You might be able to use a rejected kidney for C4d if it isn't to necrotic.
Transplant heart and liver can also be used for rejection antibodies.
JB


On Wednesday, April 1, 2015, Paula Sicurello <patpxs <@t> gmail.com> wrote:

> Good Afternoon Netters,
>
> Since we went down the path of hot dogs and Slim Jim's as positive controls
> for FFPE stains, I was wondering.....
>
> Is there a source, like a hot dog or piece of steak, that could be a
> positive control for immunofluorescence C4d?
>
> What I'd really like to find is some food or food product (our positive
> patient biopsies for the frozen IF are teeny-tiny) that is positive for the
> whole host of IF stains:  IgG, IgA, IgM, Kappa, Lambda, you get the
> picture.
>
> Please send your suggestions my way.
>
> Thanks in advance,
>
> Paula  :-)
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-- 
Jim Burchette
"Fly Fishing Bum"  *<'(((><*