[Histonet] DAKO Omnis vs Ventana Ultra

[Hinsinger Julie]

Well, Omnis can use different antibodies but problem is that manual titration can’t be done whereas it is possible on the Ultra.

To run new antibodies on the Omnis, we’ll have to use a 2mL tube with a bare code, prepare a dilution for 200uL per slide, consider a dead volume of 100uL. It is less flexible in a way, but each reagent is listed and protocol set up is saved for every step. On the Ultra or Disco from Ventana, we do a 100uL dilution per slide, we open the slide tray and do our manual titration. No other information is saved in the software but it is easy to try a small volume for a starting dilution.

We just had a demo with the Omnis. Very interesting but we need users feedback.

Thanks a lot Tina.
julie

De : Tina Van Meter [mailto:tina.vanmeter <@t> gmail.com]
Envoyé : 1 avril 2015 14:30
À : Hinsinger Julie
Cc : histonet <@t> lists.utsouthwestern.edu
Objet : Re: [Histonet] DAKO Omnis vs Ventana Ultra

Hi Julie,
I would go with the most flexible "open" system that is capable of using a wide range of protocols. (ex. antibodies from different vendors, varied section thickness, fixed frozen sections, etc.)

Kind regards,
Tina Van Meter ​

On Wed, Apr 1, 2015 at 1:19 PM, Hinsinger Julie <julie.hinsinger <@t> umontreal.ca<mailto:julie.hinsinger <@t> umontreal.ca>> wrote:
Hi,

Does anyone know about some lab using DAKO Omnis for research ?
If some of you tried the Omnis and the Ultra and would like to share about their own experience, it would be highly appreciated.
LCS vs dynamic gap staining ? Software usage ? Costs ? Service ? Reagents and kits ? Maintenance, decontamination ?

Best regards,

Julie Hinsinger
Histology Facility
Institute for Research in Immunology and Cancer (IRIC) _Université de Montréal
2950, chemin Polytechnique
Pavillon Marcelle Coutu - Local 3440
H3T 1J4, Montréal, QC

(514) 343-6111 #0503<tel:%28514%29%20343-6111%20%230503>
julie.hinsinger <@t> umontreal.ca<mailto:julie.hinsinger <@t> umontreal.ca><mailto:julie.hinsinger <@t> umontreal.ca<mailto:julie.hinsinger <@t> umontreal.ca>>



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