[Histonet] Negative Control for Validation

[Arrington, Karla A]

We are beginning the process of validating new antibodies using the BenchMark XT but have some questions for validation.

Question:  For a positive reagent control that stains appropriately positive, is a known control block used? If not, what is used?
Question:  For a negative reagent control that stains appropriately negative, what is ran?
Question:  Is a negative tissue slide ran for all of our 10 cases per antibody, or per case that will be used for validation?

Thanks All!
Karla

Karla Arrington, HT(ASCP), HIT(AHIMA)
Lead Histology Technician
ANMC Pathology
907-729-1810
kaarrington <@t> anthc.org