[Histonet] Negative Control for Validation

Arrington, Karla A kaarrington <@t> anthc.org
Mon Sep 29 13:24:45 CDT 2014

We are beginning the process of validating new antibodies using the BenchMark XT but have some questions for validation.

Question:  For a positive reagent control that stains appropriately positive, is a known control block used? If not, what is used?
Question:  For a negative reagent control that stains appropriately negative, what is ran?
Question:  Is a negative tissue slide ran for all of our 10 cases per antibody, or per case that will be used for validation?

Thanks All!

Karla Arrington, HT(ASCP), HIT(AHIMA)
Lead Histology Technician
ANMC Pathology
kaarrington <@t> anthc.org

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