[Histonet] double-staining / multi-color / co-localization IHC

[Kiousis, Sam]

Does anyone have any experience with double-staining / multi-color / co-localization immunohistochemistry?  Any protocols or kits you have used in the past would be most helpful.  I'm attempting to use 2 antibodies, CD68 and HER2, that have worked well in the past individually and combine them together on PFA-fixed, OCT frozen blocks of human brain tumor specimens.

Thanks in advance,

Sam Kiousis
Neuro-Oncology
Karmanos Cancer Institute
skiousis <@t> med.wayne.edu<mailto:skiousis <@t> med.wayne.edu>


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