[Histonet] double-staining / multi-color / co-localization IHC

Kiousis, Sam skiousis <@t> med.wayne.edu
Fri Sep 26 12:33:41 CDT 2014

Does anyone have any experience with double-staining / multi-color / co-localization immunohistochemistry?  Any protocols or kits you have used in the past would be most helpful.  I'm attempting to use 2 antibodies, CD68 and HER2, that have worked well in the past individually and combine them together on PFA-fixed, OCT frozen blocks of human brain tumor specimens.

Thanks in advance,

Sam Kiousis
Karmanos Cancer Institute
skiousis <@t> med.wayne.edu<mailto:skiousis <@t> med.wayne.edu>


This document may include proprietary and confidential information. This document may not be reproduced, copied, distributed, published, modified or furnished to third parties, without prior written consent. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. If you are not the intended recipient you are notified that disclosing, copying, distributing or taking any action in reliance on the contents of this information is strictly prohibited.

More information about the Histonet mailing list