[Histonet] Gram stain not working consistently: how likely is it that crystal violet has gone "bad"

[Jorge A. Santiago-Blay]

Dear Histoneters:

If Gram stains are not always working consistently: how likely is it that
crystal violet has gone "bad". My first suspect is the decoloration step,
then age of the culture, then thickness of the smear.  However, I do not
want to disregard any possibility, such as bad reagents. Please, email any
feedback directly to me, blayjorge <@t> gmail.com.

Cheers and gracias,

Jorge

Jorge A. Santiago-Blay, PhD
blaypublishers.com
http://blayjorge.wordpress.com/
http://paleobiology.si.edu/staff/individuals/santiagoblay.html