[Histonet] Mohs

[Anne Murvosh]

On fatty tissue, especially cheeks. After we mount on a slide we spray the tissue with liquid nitrogen and let sit to freeze before embedding for a couple of minutes.  Than after embedding spray the chuck again with liquid nitrogen.  On really fatty stuff you may have to freeze before cutting each time. Time consuming yes.  But I find freezing and let sitting I don't fight with it as much when cutting.  Anne

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of michele carr
Sent: Tuesday, September 23, 2014 9:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mohs

Hello I perform mohs cutting a few days a week and I noticed that on fatty skin tissue I don't get the interior of the skin section. I only seem to get the epidermis. Is there a trick to getting the fatty tissue too. I tried bumping up the microns but that only helped a bit.
Thanks for your responses.

Michele Carr
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