[Histonet] RE: Artifact in IHC

Teri Johnson tejohnson <@t> genoptix.com
Mon Sep 22 12:17:32 CDT 2014

Hi Donna,

Interesting conundrum! A few questions:
 - Do you see it in tissues that have not undergone any staining, just floated in buffer and coverslipped?
 - Have you tried wet mounting them after staining and prior to dehydration and clearing to see if the artifact is there?
 - Have you recently changed glove manufacturers, could this be talc or some other powder from gloves?
 - Are you dehydrating out of PBS into an alcohol concentration higher than 70%? You could be getting phosphate precipitation. Try using a water rinse prior to alcohol or make sure your first alcohol concentration is 70% or lower.

Those are the weird things that came to mind. I hope this helps,

Teri Johnson
Manager Clinical Trial Testing
Genoptix, Inc.
Carlsbad, CA 92008


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