[Histonet] Blades for cutting resin on a microtome

[Patsy Ruegg]

I used to have a triangle glass knife holder insert for my Leica microtome or I would use the tungsten carbide knives.  It depends on what you are cutting.  if it is calcified bone the glass knives scratch too much and they are only 1/2 inch wide so you have to cut smaller soft tissues with them.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm <@t> hotmail.com
pruegg <@t> ihctech.net


From: abright <@t> brightinstruments.com
Date: Fri, 12 Sep 2014 17:32:47 +0100
To: GKeyser <@t> uwhealth.org
Subject: Re: [Histonet] Blades for cutting resin on a microtome
CC: histonet <@t> lists.utsouthwestern.edu; veronique.barres <@t> gmail.com; export <@t> brightinstruments.com

Yes brightinstruments, com make glass knife holder and tungsten carbide tipped knives for microtomes,
KR,,,,,Alan Bright
 
Sent from my iPhone
 
> On 12 Sep 2014, at 15:49, "Keyser Gerald  T" <GKeyser <@t> uwhealth.org> wrote:
> 
> I've only cut resin with a glass or diamond knife in an ultramicrotome. If you are attempting to do it in a regular microtome, you would need a special blade holder. I don't know if any microtome manufactures make glass knife holders. 
> 
> You make the glass blades yourself using special glass. Here is a link to the glass strips: http://www.sigmaaldrich.com/catalog/product/sigma/g2528?lang=en&region=US
> 
> Here is a cheap jig and diamond glass cutters it make the knifes:
> https://www.emsdiasum.com/microscopy/products/preparation/glassknife.aspx
> 
> I've never made glass knives by hand using a hand held diamond cutter and jigs. I imagine that it would take practice.  
> 
> I've only used a specialized maker:
> https://www.emsdiasum.com/microscopy/products/histology/tissue_stainer.aspx
> 
> You paint a bit of nail polish underneath the glass edge and put a bit of distilled water on the edge. You then section the block floating the sections on the water. Use an eyelash manipulator to pick up the 5um thick sections and place on a bubble of water on the slide. Evaporate the water droplet on the slide. If you've done it right, the sections won't look like origami. If it does, then practice until it doesn't. 
> 
> Gerry 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Véronique Barrès
> Sent: Friday, September 12, 2014 9:33 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Blades for cutting resin on a microtome
> 
> Happy Friday Histonetters!
> 
> I am working on a histology platform in a research center and someone came to me last week and asked to cut blocs of resin (JB-4 resin) on the microtome. I never cut anything else than paraffin, so I was wondering if some of you had advices for me?
> 
> They never did it neither and took their protocol in a paper where it was said that we should use disposable glass knife instead of standard metal blades. Are any of you ever used those knife? Where do you buy them?
> We have an old Leica RM2125.
> 
> Thanks for your advices!
> 
> Véronique
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