[Histonet] RE: papillary dermis

[Morken, Timothy]

Kris, reticular dermis has elastic fibers, papillary dermis does not. So an elastic stain will show fibers in the reticular  area only. 

You can use IHC for collagen types as well. For instance, start with:

http://www.derm101.com/inflammatory/embryologic-histologic-and-anatomic-aspects/collagen/


If you are looking for a bright-line demarcation I think you will be disappointed. There is a gradation between the two so about the best you can do is say the change-over is in a narrow band of the dermis.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher
Sent: Thursday, September 11, 2014 9:33 AM
To: histonet <@t> lists.utsouthwestern.edu; histonet-request <@t> lists.utsouthwestern.edu
Subject: [Histonet] papillary dermis

Hello All,

Does anyone know of a technique, special stain, biomarker, etc.  that will somehow demonstrate the difference between papillary dermis and reticular dermis in skin.  Estimating where the reticular dermis ends and the reticular dermis ends is very subjective.  Any help would be greatly appreciated.  Thanks in advance.

Kris
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