[Histonet] Sirius Red (Normal Light) Yellow Colour Degradation

Grantham, Andrea L - (algranth) algranth <@t> email.arizona.edu
Tue Sep 2 10:25:27 CDT 2014

I've been doing this stain for years and the protocol that I use came from John Kiernan (Histonet Archives). If you use Sirius Red F3B (CI 35782) the stain will be good for up to 3 years. Don't use a dye that does not have this CI #.
I have used the stain over and over and it always produces excellent results. I stain for one hour in the PSR, wash in two quick changes of Acidified Water. I usually do not do the hematoxylin step because the researchers requesting the stain prefer it that way. The tissue I stain most often with PSR is carotid and/or aorta and heart but I have done a beautiful stain on the back of the eye including optic nerve. With polarized light it is a thing of beauty to observe.

Andi G

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415     Fax: 520.626.2097

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