From pathdynamo <@t> gmail.com  Mon Sep  1 12:31:38 2014
From: pathdynamo <@t> gmail.com (Path Dynamo)
Date: Mon Sep  1 12:31:50 2014
Subject: [Histonet] RE: CD4 1F6 clone (Steven Weston)
Message-ID: <k3yfmhgkmw5ybk3v2fi8mepn.1409592698505@email.android.com>

We use this clone for rhesus macaques FFPE and it works. Not the best but still works.
We do 1:20 dilution, ?60' incubation, high pH 9.0 HIER (BondRX or Biocare chamber), and H2O2 block before protein block.
We know it works better on frozen than on FFPE.

I hope this helps.
Best
Pathology Dynamics

<div>-------- Original message --------</div><div>From: histonet-request@lists.utsouthwestern.edu </div><div>Date:09/01/2014  10:00 AM  (GMT-08:00) </div><div>To: histonet@lists.utsouthwestern.edu </div><div>Subject: Histonet Digest, Vol 130, Issue 1 </div><div>
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Today's Topics:

   1. CD4 1F6 clone (Steven Weston)


----------------------------------------------------------------------

Message: 1
Date: Sun, 31 Aug 2014 23:49:20 +0000
From: Steven Weston <Steven.Weston@utas.edu.au>
Subject: [Histonet] CD4 1F6 clone
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Message-ID: <D029F19F.1D09E%Steven.Weston@utas.edu.au>
Content-Type: text/plain; CHARSET=US-ASCII

I also tried it without H2O2 with no result either.
Anyone else had these problems with this clone ???
Interestingly in the past I used the duo pack of CD4/8 that had the 1F6 for cd4 and the 4B11 clone of cd8 and they both worked.
May have to try the 4b12 clone for cd4.
regards
Steve Weston

Message: 10
Date: Sat, 30 Aug 2014 15:44:09 +0530
From: Neeraj Singh <neerajbioxford@gmail.com<mailto:neerajbioxford@gmail.com>>
Subject: Re: [Histonet] Re cd4 1F6 clone
To: Steven Weston <Steven.Weston@utas.edu.au<mailto:Steven.Weston@utas.edu.au>>
Cc: "histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>"
<histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>>
Message-ID: <1663FC3E-E152-411F-B553-83D3D426ACEE@gmail.com<mailto:1663FC3E-E152-411F-B553-83D3D426ACEE@gmail.com>>
Content-Type: text/plain; charset=us-ascii

Try with no H2O2 at all for this clone. it will hill you.

Thanks

Neeraj


On Aug 30, 2014, at 1:40 PM, Steven Weston <Steven.Weston@utas.edu.au<mailto:Steven.Weston@utas.edu.au>> wrote:

Hi Histonetters
Has anyone else had difficulty getting  this clone to work? cd4 1F6 from leica microsystems (formerly novocastra).
I've tried just about every trick in my book and still no staining.
Particularly i made sure i did the peroxidase block prior to HIER (i even tried using it after the primary) and have tried low, high and middle pH buffers with antibody concentrations from 1/10-1/40 for 60 and 90 mins and use envision plus detection with DAB plus. I've had two batches of this over the last ten years and neither has worked.
Anyone else with the same experience ?
regards
steve weston
lab manager
Breathe-Well CRE
UTAS-SOM

Steve Weston
Lab Manager
Breathe-Well Centre of Research Excellence for Chronic Respiratory Disease.
UTAS-SOM
0408990859
62264871




------------------------------

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End of Histonet Digest, Vol 130, Issue 1
****************************************
From BDeBrosse-Serra <@t> isisph.com  Tue Sep  2 09:50:46 2014
From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra)
Date: Tue Sep  2 09:51:00 2014
Subject: [Histonet] Sirius Red (Normal Light) Yellow Colour Degradation
In-Reply-To: <232D1176-4C09-4962-9733-51AD562D732E@mail.mcgill.ca>
References: <232D1176-4C09-4962-9733-51AD562D732E@mail.mcgill.ca>
Message-ID: <CB69CD3FBEB5524CA851930C971288FD329C373C@Exch10MB02.isis.local>

We always make the solution up fresh. 

Bea

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andr? Charbonneau
Sent: Friday, August 29, 2014 4:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sirius Red (Normal Light) Yellow Colour Degradation

Hello to all, 

This is a quick comment I would like to mention to people completing the Sirius Red staining. If you don't seem to have the desired yellow cytoplasm ( I have seen in axolotl red blood cells) then the issue might be because you Sirius Red liquid is not longer effective. I know this might seem trivial but I was reading some previous posts that mention that the solution is good for up to 12 years. Personally, I have stained several hundred slides with the same solution of ~ 250ml of Sirius Red and gradually, the yellow found in the muscle and in the cytoplasm in normal light started becoming less vibrant. The solution can probably be kept for 12 years but it has limited amounts of usage. Once I re-made my solution, the yellow cytoplasm and muscles were vibrantly yellow again. This is different from other troubleshooting comments that were posted earlier which said to keep the slides in a shorter amount of time in the alcohols during the dehydration. Simply changing the Sirius Red would be the way to go. Both old Sirius Red and new were compared at the same time using same alcohols and acetic acids, thus removing the probability that other factors affected the colouring. 

Sirius Red 0.1% in Sat Sol. of Picric Acid: 
Direct Red 80 0.1g
Sat Sol. Picric Acid 100ml


Andr? Charbonneau
M.Sc. Candidate
Faculty of Dentistry 
McGill University




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From Vickroy.Jim <@t> mhsil.com  Tue Sep  2 09:51:26 2014
From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim)
Date: Tue Sep  2 09:51:31 2014
Subject: [Histonet] Frozen sections for Immunoflouresence
Message-ID: <BB0B9F1A8373F14FA2974E8CB24BF9CFC353B7C7@mmc-mail.ad.mhsil.com>

Lately we are experiencing an issue where the tissue sections are coming off of the slide during the staining process.   We have always used positive charged slides and have not experienced this problem on a routine case.   We do not fix the slides but allow them to air dry for Immunology.  Some have suggested a quick fixation in acetone or 95% ETOH.   This doesn't seem to correct the problem and we don't find the IF stains work as well.   We have tried a different batch of slides and are looking into a different kind of coating for the slides.   Our regular frozen sections stay on the slides.  We are using OCT.   The only other thing I have noticed is that maybe it's my imagination but the OCT in the block surrounding the kidney biopsy seems a little softer than normal.   I did try a different mounting medium and maybe the softness was my imagination.   Has anyone else experienced this problem and if so how did you correct it?   The lab is air conditioned so I don't think the humidity is the main factor.  The Immunology staff changed all of their reagents so we have reduced that variable hopefully.   We are using Leica positive charged slides.  (I have found that the Apex control box slides from Leica are a little better than the regular Apex slides.   Any ideas?

Jim

________________________________
This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited.
From jfinley <@t> wakehealth.edu  Tue Sep  2 10:12:38 2014
From: jfinley <@t> wakehealth.edu (Joseph Finley)
Date: Tue Sep  2 10:12:49 2014
Subject: [Histonet] CD34 NHP Tissue
Message-ID: <08E3F6B222E95145BB62A542C7F1015C1681EE0A@EXCHDB2.medctr.ad.wfubmc.edu>

Does anyone know of a good CD34 antibody for FFPE IHC that works on non-human primate tissue - specifically Macaca fascicularis?

Joseph Wayne Finley II
Laboratory Technician III, HTL (ASCP)cm

Department of Comparative Medicine
Medical Center Blvd \ Winston-Salem, NC  27157-1040
(336) 716-1536
jfinley@wakehealth.edu<mailto:jfinley@wakehealth.edu>


From algranth <@t> email.arizona.edu  Tue Sep  2 10:25:27 2014
From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth))
Date: Tue Sep  2 10:25:32 2014
Subject: [Histonet] Sirius Red (Normal Light) Yellow Colour Degradation
In-Reply-To: <CB69CD3FBEB5524CA851930C971288FD329C373C@Exch10MB02.isis.local>
References: <232D1176-4C09-4962-9733-51AD562D732E@mail.mcgill.ca>
	<CB69CD3FBEB5524CA851930C971288FD329C373C@Exch10MB02.isis.local>
Message-ID: <F88AA0D8-882F-411A-90C3-710E411BB2E3@email.arizona.edu>

I've been doing this stain for years and the protocol that I use came from John Kiernan (Histonet Archives). If you use Sirius Red F3B (CI 35782) the stain will be good for up to 3 years. Don't use a dye that does not have this CI #.
I have used the stain over and over and it always produces excellent results. I stain for one hour in the PSR, wash in two quick changes of Acidified Water. I usually do not do the hematoxylin step because the researchers requesting the stain prefer it that way. The tissue I stain most often with PSR is carotid and/or aorta and heart but I have done a beautiful stain on the back of the eye including optic nerve. With polarized light it is a thing of beauty to observe.


Andi G

Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth@email.arizona.edu<mailto:algranth@email.arizona.edu>
Tel: 520.626.4415     Fax: 520.626.2097





From bburnett <@t> CapeCodHealth.org  Tue Sep  2 10:29:49 2014
From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy)
Date: Tue Sep  2 10:29:54 2014
Subject: [Histonet] RE: Frozen sections for Immunoflouresence
In-Reply-To: <BB0B9F1A8373F14FA2974E8CB24BF9CFC353B7C7@mmc-mail.ad.mhsil.com>
References: <BB0B9F1A8373F14FA2974E8CB24BF9CFC353B7C7@mmc-mail.ad.mhsil.com>
Message-ID: <C7CEB8BE7DF69446817C237018344D0004C125@FHEXMB1.cchdomain1.capecodhealth.org>

Are you using pre-cut control slides?

________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Vickroy, Jim [Vickroy.Jim@mhsil.com]
Sent: Tuesday, September 02, 2014 10:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen sections for Immunoflouresence

Lately we are experiencing an issue where the tissue sections are coming off of the slide during the staining process.   We have always used positive charged slides and have not experienced this problem on a routine case.   We do not fix the slides but allow them to air dry for Immunology.  Some have suggested a quick fixation in acetone or 95% ETOH.   This doesn't seem to correct the problem and we don't find the IF stains work as well.   We have tried a different batch of slides and are looking into a different kind of coating for the slides.   Our regular frozen sections stay on the slides.  We are using OCT.   The only other thing I have noticed is that maybe it's my imagination but the OCT in the block surrounding the kidney biopsy seems a little softer than normal.   I did try a different mounting medium and maybe the softness was my imagination.   Has anyone else experienced this problem and if so how did you correct it?   The lab is air conditioned so I don't think the humidity is the main factor.  The Immunology staff changed all of their reagents so we have reduced that variable hopefully.   We are using Leica positive charged slides.  (I have found that the Apex control box slides from Leica are a little better than the regular Apex slides.   Any ideas?

Jim

________________________________
This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited.
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From jkrupp <@t> deltacollege.edu  Tue Sep  2 11:00:00 2014
From: jkrupp <@t> deltacollege.edu (Jon Krupp)
Date: Tue Sep  2 11:00:13 2014
Subject: [Histonet] Non-cert. histo classes
Message-ID: <1BA185E7-E5D4-4B0D-8CCC-978212072356@deltacollege.edu>

Greetings

I am a regular reader of this list, but from a peripheral setting.

I work in an electron microscopy certificate program. Our students are prepared to do either or both certificates in EM, biological and materials.

During their biological training, students are instructed in thin sectioning, specimen prep., etc. I would like to get ideas about how valuable adding more light microscopy specimen prep, staining, and sectioning might be for these students. Would it be good for them to have these skills and knowledge but not a histotech cert.?

I thought about doing a full cert. program, but am daunted by the requirements to set one up and I don't want to compete with other better established programs near by.

Bottom line is I would like to know if teaching basic histo tech skills, w/o certification, is a viable path. Could students leverage these skills into jobs at non-health care type facilities? Could having these skill help them complete a certificated program if they wanted to take that direction later? 

Your input will be valuable to both me and my students.

Thanks

Jon

Jonathan Krupp
Applied Science, Business & Technology
San Joaquin Delta College
5151 Pacific Ave.
Stockton, CA  95207
209-954-5284
jkrupp@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College









From flnails <@t> texaschildrens.org  Tue Sep  2 12:00:21 2014
From: flnails <@t> texaschildrens.org (Nails, Felton)
Date: Tue Sep  2 12:00:29 2014
Subject: [Histonet] Non-cert. histo classes
In-Reply-To: <1BA185E7-E5D4-4B0D-8CCC-978212072356@deltacollege.edu>
References: <1BA185E7-E5D4-4B0D-8CCC-978212072356@deltacollege.edu>
Message-ID: <327E034F1892504289B7A17EC71DF9F308DEFA2E@TCFMSG03.ad.texaschildrenshospital.org>

My opinion is that it is a good idea because many hospitals are shutting down their EM department because of a lack of skilled workers.
So the more skills they have the better for them.
Let me know if any of your students want to move to Houston Texas.

Felton Nails
Texas Children's Hospital 
832-824-1248

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Krupp
Sent: Tuesday, September 02, 2014 11:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Non-cert. histo classes

Greetings

I am a regular reader of this list, but from a peripheral setting.

I work in an electron microscopy certificate program. Our students are prepared to do either or both certificates in EM, biological and materials.

During their biological training, students are instructed in thin sectioning, specimen prep., etc. I would like to get ideas about how valuable adding more light microscopy specimen prep, staining, and sectioning might be for these students. Would it be good for them to have these skills and knowledge but not a histotech cert.?

I thought about doing a full cert. program, but am daunted by the requirements to set one up and I don't want to compete with other better established programs near by.

Bottom line is I would like to know if teaching basic histo tech skills, w/o certification, is a viable path. Could students leverage these skills into jobs at non-health care type facilities? Could having these skill help them complete a certificated program if they wanted to take that direction later? 

Your input will be valuable to both me and my students.

Thanks

Jon

Jonathan Krupp
Applied Science, Business & Technology
San Joaquin Delta College
5151 Pacific Ave.
Stockton, CA  95207
209-954-5284
jkrupp@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College









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From Timothy.Morken <@t> ucsfmedctr.org  Tue Sep  2 12:35:20 2014
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy)
Date: Tue Sep  2 12:36:36 2014
Subject: [Histonet] Non-cert. histo classes
In-Reply-To: <1BA185E7-E5D4-4B0D-8CCC-978212072356@deltacollege.edu>
References: <1BA185E7-E5D4-4B0D-8CCC-978212072356@deltacollege.edu>
Message-ID: <761E2B5697F795489C8710BCC72141FF36796206@ex07.net.ucsf.edu>

Jon,

It is not a bad idea. Ideally a person going into the field would have a good formal education in the field. However, 99.9% of the people working in the field did not go through a formal program, but learned on the job. Therefore, a person who was exposed to ANY formal education in paraffin histotechnology (processing, cutting, staining, special stains) would be well ahead- OJT is highly variable in quality as you might guess. A lot of what is learned in biological EM is transferable to paraffin - fixation, processing, even sectioning principles are the same. The difference is really in medium and staining chemicals, and of course, the microscope used. 

There are obviously a lot more jobs in histology than in EM. Biotech does not necessarily require certification and it is not needed as a regulatory requirement of their work (and a combined EM/histotech is more valuable; throw in some DNA/RNA work (ISH, FISH, PCR and you have a supertech!). Hospitals and other medical labs usually do not require certification (like or not!) for entry level (or even higher levels in many cases) but if they want you to have it they will usually have a time period that they require you to get it - "certification eligible" or "certification within one year" or something like that. Many of our histotechs came from the UCSF research labs where they learned a bit of paraffin sectioning and then applied in our lab. All have done well and all have gone on to get certified.

Acquiring certification requires working one year under a board certified pathologist, and taking a test. It takes some study, but that is the route most people take.

The most important part is that certification now requires an AA degree at the minimum with certain levels of biology and chemistry courses. Those at Delta would meet that standard pretty easily if they are in the EM program anyway. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Krupp
Sent: Tuesday, September 02, 2014 9:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Non-cert. histo classes

Greetings

I am a regular reader of this list, but from a peripheral setting.

I work in an electron microscopy certificate program. Our students are prepared to do either or both certificates in EM, biological and materials.

During their biological training, students are instructed in thin sectioning, specimen prep., etc. I would like to get ideas about how valuable adding more light microscopy specimen prep, staining, and sectioning might be for these students. Would it be good for them to have these skills and knowledge but not a histotech cert.?

I thought about doing a full cert. program, but am daunted by the requirements to set one up and I don't want to compete with other better established programs near by.

Bottom line is I would like to know if teaching basic histo tech skills, w/o certification, is a viable path. Could students leverage these skills into jobs at non-health care type facilities? Could having these skill help them complete a certificated program if they wanted to take that direction later? 

Your input will be valuable to both me and my students.

Thanks

Jon

Jonathan Krupp
Applied Science, Business & Technology
San Joaquin Delta College
5151 Pacific Ave.
Stockton, CA  95207
209-954-5284
jkrupp@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College









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From mucram11 <@t> comcast.net  Tue Sep  2 13:08:54 2014
From: mucram11 <@t> comcast.net (Pam Marcum)
Date: Tue Sep  2 13:09:11 2014
Subject: [Histonet] Non-cert. histo classes
In-Reply-To: <761E2B5697F795489C8710BCC72141FF36796206@ex07.net.ucsf.edu>
References: <1BA185E7-E5D4-4B0D-8CCC-978212072356@deltacollege.edu>
	<761E2B5697F795489C8710BCC72141FF36796206@ex07.net.ucsf.edu>
Message-ID: <178336186.4130836.1409681334364.JavaMail.root@comcast.net>

I agree with Tim the more you know the better off you are and if you have some skills in Histology and can do EM that is a plus.? Any form of molecular is a plus now and many companies do not even require certification in any field as long as you have the science and background to do the work.? It is a rare thing to find someone who can do it all in the microscopy field and I would think a good set of skills to be able to present.? 
? 
EM appears to be in the process of becoming more useful again.? When I learned EM years ago it was in a lab with some help from an EM person in another department and on my own.? Thank goodness I was too young and dumb to know how daunting it could have been to go that route.? Unfortuantely I have not used those skills in many years.? 
? 
Pam Marcum 
UAMS 

----- Original Message -----

From: "Timothy Morken" <Timothy.Morken@ucsfmedctr.org> 
To: "Jon Krupp" <jkrupp@deltacollege.edu>, "Histonet" <histonet@lists.utsouthwestern.edu> 
Sent: Tuesday, September 2, 2014 12:35:20 PM 
Subject: RE: [Histonet] Non-cert. histo classes 

Jon, 

It is not a bad idea. Ideally a person going into the field would have a good formal education in the field. However, 99.9% of the people working in the field did not go through a formal program, but learned on the job. Therefore, a person who was exposed to ANY formal education in paraffin histotechnology (processing, cutting, staining, special stains) would be well ahead- OJT is highly variable in quality as you might guess. A lot of what is learned in biological EM is transferable to paraffin - fixation, processing, even sectioning principles are the same. The difference is really in medium and staining chemicals, and of course, the microscope used. 

There are obviously a lot more jobs in histology than in EM. Biotech does not necessarily require certification and it is not needed as a regulatory requirement of their work (and a combined EM/histotech is more valuable; throw in some DNA/RNA work (ISH, FISH, PCR and you have a supertech!). Hospitals and other medical labs usually do not require certification (like or not!) for entry level (or even higher levels in many cases) but if they want you to have it they will usually have a time period that they require you to get it - "certification eligible" or "certification within one year" or something like that. Many of our histotechs came from the UCSF research labs where they learned a bit of paraffin sectioning and then applied in our lab. All have done well and all have gone on to get certified. 

Acquiring certification requires working one year under a board certified pathologist, and taking a test. It takes some study, but that is the route most people take. 

The most important part is that certification now requires an AA degree at the minimum with certain levels of biology and chemistry courses. Those at Delta would meet that standard pretty easily if they are in the EM program anyway. 

Tim Morken 
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies 
UC San Francisco Medical Center 
San Francisco, CA 


-----Original Message----- 
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Krupp 
Sent: Tuesday, September 02, 2014 9:00 AM 
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Non-cert. histo classes 

Greetings 

I am a regular reader of this list, but from a peripheral setting. 

I work in an electron microscopy certificate program. Our students are prepared to do either or both certificates in EM, biological and materials. 

During their biological training, students are instructed in thin sectioning, specimen prep., etc. I would like to get ideas about how valuable adding more light microscopy specimen prep, staining, and sectioning might be for these students. Would it be good for them to have these skills and knowledge but not a histotech cert.? 

I thought about doing a full cert. program, but am daunted by the requirements to set one up and I don't want to compete with other better established programs near by. 

Bottom line is I would like to know if teaching basic histo tech skills, w/o certification, is a viable path. Could students leverage these skills into jobs at non-health care type facilities? Could having these skill help them complete a certificated program if they wanted to take that direction later? 

Your input will be valuable to both me and my students. 

Thanks 

Jon 

Jonathan Krupp 
Applied Science, Business & Technology 
San Joaquin Delta College 
5151 Pacific Ave. 
Stockton, CA ?95207 
209-954-5284 
jkrupp@deltacollege.edu 

Find us on Facebook @ 
Electron Microscopy at SJ Delta College 









_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

From JMacDonald <@t> mtsac.edu  Tue Sep  2 13:14:12 2014
From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald)
Date: Tue Sep  2 13:14:20 2014
Subject: [Histonet] Non-cert. histo classes
In-Reply-To: <761E2B5697F795489C8710BCC72141FF36796206@ex07.net.ucsf.edu>
References: <1BA185E7-E5D4-4B0D-8CCC-978212072356@deltacollege.edu>
	<761E2B5697F795489C8710BCC72141FF36796206@ex07.net.ucsf.edu>
Message-ID: <OF70F667A4.CF106DCA-ON88257D47.00638B79-88257D47.00640278@mtsac.edu>

I agree with Tim.  Any additional skills in histology will make them more 
marketable.  EM and paraffin microtomy are very similar in principle.

As of yet an AA/A is not required to take the HT (ASCP) certification 
exam.  Route 1 states "graduation from a NAACLS accredited program" and 
there are still some NAACLS accredited programs that offer a certificate 
and do not require an AA or AS.  The NSH appealed to NAACLS to change one 
of the Standards to require all NAACLS HT programs to either award an 
AA/AS degree or require students to have one before earning the 
certificate.  The changes to the standard went out for public comment at 
the beginning of the summer.  The NAACLS BOD meeting is September 18-19. 
The Board will vote then to either recommend or not recommend the changes 
to the standard.  If it is approved by the BOD there will be a transition 
period to allow those programs that do not offer or require an AA/AS to 
meet the standard.This does not affect those that are already certified, 
nor does it change the ASCP requirements.

Jennifer



From:   "Morken, Timothy" <Timothy.Morken@ucsfmedctr.org>
To:     "'Jon Krupp'" <jkrupp@deltacollege.edu>, 
"histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>
Date:   09/02/2014 10:38 AM
Subject:        RE: [Histonet] Non-cert. histo classes
Sent by:        histonet-bounces@lists.utsouthwestern.edu



Jon,

It is not a bad idea. Ideally a person going into the field would have a 
good formal education in the field. However, 99.9% of the people working 
in the field did not go through a formal program, but learned on the job. 
Therefore, a person who was exposed to ANY formal education in paraffin 
histotechnology (processing, cutting, staining, special stains) would be 
well ahead- OJT is highly variable in quality as you might guess. A lot of 
what is learned in biological EM is transferable to paraffin - fixation, 
processing, even sectioning principles are the same. The difference is 
really in medium and staining chemicals, and of course, the microscope 
used. 

There are obviously a lot more jobs in histology than in EM. Biotech does 
not necessarily require certification and it is not needed as a regulatory 
requirement of their work (and a combined EM/histotech is more valuable; 
throw in some DNA/RNA work (ISH, FISH, PCR and you have a supertech!). 
Hospitals and other medical labs usually do not require certification 
(like or not!) for entry level (or even higher levels in many cases) but 
if they want you to have it they will usually have a time period that they 
require you to get it - "certification eligible" or "certification within 
one year" or something like that. Many of our histotechs came from the 
UCSF research labs where they learned a bit of paraffin sectioning and 
then applied in our lab. All have done well and all have gone on to get 
certified.

Acquiring certification requires working one year under a board certified 
pathologist, and taking a test. It takes some study, but that is the route 
most people take.

The most important part is that certification now requires an AA degree at 
the minimum with certain levels of biology and chemistry courses. Those at 
Delta would meet that standard pretty easily if they are in the EM program 
anyway. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special 
Studies
UC San Francisco Medical Center
San Francisco, CA


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [
mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Krupp
Sent: Tuesday, September 02, 2014 9:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Non-cert. histo classes

Greetings

I am a regular reader of this list, but from a peripheral setting.

I work in an electron microscopy certificate program. Our students are 
prepared to do either or both certificates in EM, biological and 
materials.

During their biological training, students are instructed in thin 
sectioning, specimen prep., etc. I would like to get ideas about how 
valuable adding more light microscopy specimen prep, staining, and 
sectioning might be for these students. Would it be good for them to have 
these skills and knowledge but not a histotech cert.?

I thought about doing a full cert. program, but am daunted by the 
requirements to set one up and I don't want to compete with other better 
established programs near by.

Bottom line is I would like to know if teaching basic histo tech skills, 
w/o certification, is a viable path. Could students leverage these skills 
into jobs at non-health care type facilities? Could having these skill 
help them complete a certificated program if they wanted to take that 
direction later? 

Your input will be valuable to both me and my students.

Thanks

Jon

Jonathan Krupp
Applied Science, Business & Technology
San Joaquin Delta College
5151 Pacific Ave.
Stockton, CA  95207
209-954-5284
jkrupp@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College









_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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From Vickroy.Jim <@t> mhsil.com  Tue Sep  2 13:31:14 2014
From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim)
Date: Tue Sep  2 13:31:20 2014
Subject: [Histonet] Adhesive coated slides verses positive charged slides
Message-ID: <BB0B9F1A8373F14FA2974E8CB24BF9CFC353B820@mmc-mail.ad.mhsil.com>

Does anyone have any experience on which slides work better for frozen sections and immunofluorescence stains......adhesive coated or positive charged?

Does anyone else find the selection of a proper slide confusing or is it just my age?   I understand the basis behind hydrophobic, hydrophilic, adhesive coated, and positive charged however we have thought our Leica Apex slides were positive charged all along.

Thanks

Jim

________________________________
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From JFerrer <@t> lifecell.com  Tue Sep  2 13:34:08 2014
From: JFerrer <@t> lifecell.com (Ferrer, Joselito)
Date: Tue Sep  2 13:34:18 2014
Subject: [Histonet] Take me out of the mailing list
Message-ID: <45k8rd4r3q959g8jjs1s9pnu.1409682847939@email.android.com>

Please take me out of the mail list.

Thank you


Sent from my Verizon Wireless 4G LTE smartphone
From Lacie.Algeo <@t> providence.org  Tue Sep  2 16:38:18 2014
From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A)
Date: Tue Sep  2 16:38:35 2014
Subject: [Histonet] RE: IHC Validation
Message-ID: <24C4B3C167E5694887AB594C7602CE3A0D0D62@WN35104.or.providence.org>

I have used the CAP published 'Quality Management in Anatomic Pathology' and it has been very helpful with this:

"For a well-characterized antibody  with a limited spectrum of antigenic targets, like chromogranin, or prostate specific antigen, the validation can be limited.  A panel of 10 positive and 10 negative neoplasms would be sufficient in this setting.  For an antibody that is not well characterized and/or has a wide range of reported activity, a more extensive validation is necessary.  The number of tissues tested should in this circumstance be large enough to determine whether the staining profile matches that previously described. "

We use cases known to be positive or negative for a given antibody or we request tumor types that are known to be positive or negative.  We do 10 of each.  If an antibody has a wide range of positivities (i.e. % positive like c-Myc or FOXP1), we try to have a range of different positivities for review.

Hope this helps,
Lacie

Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
lacie.algeo@providence.org<mailto:lacie.algeo@providence.org>


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From tony.henwood <@t> health.nsw.gov.au  Tue Sep  2 19:34:40 2014
From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN))
Date: Tue Sep  2 19:34:58 2014
Subject: [Histonet] RE: Frozen sections for Immunoflouresence
In-Reply-To: <BB0B9F1A8373F14FA2974E8CB24BF9CFC353B7C7@mmc-mail.ad.mhsil.com>
References: <BB0B9F1A8373F14FA2974E8CB24BF9CFC353B7C7@mmc-mail.ad.mhsil.com>
Message-ID: <6D6BD1DE8A5571489398B392A38A7157E01013CD@xmdb04.nch.kids>

Hi Jim,

You mention that the OCT seems a little softer.
Could it be that the sections are thicker than you usually produce. Check the Frozen section H&E to check this.
If sections are thicker they will tend to detach from the slides.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Wednesday, 3 September 2014 12:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Frozen sections for Immunoflouresence

Lately we are experiencing an issue where the tissue sections are coming off of the slide during the staining process.   We have always used positive charged slides and have not experienced this problem on a routine case.   We do not fix the slides but allow them to air dry for Immunology.  Some have suggested a quick fixation in acetone or 95% ETOH.   This doesn't seem to correct the problem and we don't find the IF stains work as well.   We have tried a different batch of slides and are looking into a different kind of coating for the slides.   Our regular frozen sections stay on the slides.  We are using OCT.   The only other thing I have noticed is that maybe it's my imagination but the OCT in the block surrounding the kidney biopsy seems a little softer than normal.   I did try a different mounting medium and maybe the softness was my imagination.   Has anyone else experienced this problem and if so how did you correct it?   The lab is air conditioned so I don't think the humidity is the main factor.  The Immunology staff changed all of their reagents so we have reduced that variable hopefully.   We are using Leica positive charged slides.  (I have found that the Apex control box slides from Leica are a little better than the regular Apex slides.   Any ideas?

Jim

________________________________
This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited.
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From paw555 <@t> yahoo.com  Wed Sep  3 09:13:25 2014
From: paw555 <@t> yahoo.com (pam plumlee)
Date: Wed Sep  3 09:13:30 2014
Subject: [Histonet] HT Job Opportunity in San Diego
Message-ID: <1409753605.7766.YahooMailBasic@web140104.mail.bf1.yahoo.com>

Full time temp to hire position in the heart of Biotech Beach.  Growing Cancer Diagnostic Lab seeks a experienced H.T. with excellent cutting skills. Great working enviroment with the opportunity to learn valuable skills.  Contact:  Pam Plumlee H.T., Lead at Biotheranostics
pam.plumlee@biotheranostics.com

From amber.mckenzie <@t> gastrodocs.net  Wed Sep  3 09:26:52 2014
From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie)
Date: Wed Sep  3 09:27:01 2014
Subject: [Histonet] QIHC test
In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A0D0D62@WN35104.or.providence.org>
References: <24C4B3C167E5694887AB594C7602CE3A0D0D62@WN35104.or.providence.org>
Message-ID: <5A33C952BB67F4468AF1F36D739212BC011252B3A8@JERRY.Gia.com>

I ordered the MSH online study guide for the QIHC, but it was only questions...no answer key in the back.  What books am I supposed to get the answers from?  Any other study suggestions? Thanks!

From Lyndsey.Buechel <@t> covance.com  Wed Sep  3 09:49:27 2014
From: Lyndsey.Buechel <@t> covance.com (Buechel, Lyndsey)
Date: Wed Sep  3 09:51:20 2014
Subject: [Histonet] Histology Professionals Needed
Message-ID: <F624D839BEA2654B94161AFF87E724DA2B108F1C@DFWPW0EXCH010.ent.covance.com>

Covance, a global contract research organization is looking for a number of histology professionals to join our team in Madison, WI. Relocation assistance is available. We offer competitive wages and excellent benefits, including 21 days of paid time off.

Supervisor (47944) - at least 3 years of lab experience. Prior supervisor experience preferred but not required.
Research Assistant (47382) - at least 3 years of histology and/or necropsy experience
Sr. Study Technician (47287) - at least 2 years of histology and/or necropsy experience.

Please visit www.covancecareers.com<http://www.covancecareers.com> to apply to the corresponding 5 digit number or reach out to me directly with any questions.

Lyndsey Buechel
Recruiter
Recruitment and Talent Advisors
Covance Inc.
Minneapolis, MN
Tel:  +1 218 297 4125
E-mail:  lyndsey.buechel@covance.com<mailto:lyndsey.buechel@covance.com>
Apply online at: www.covancecareers.com<http://www.covancecareers.com/>
________________________________
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If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you.
From blayjorge <@t> gmail.com  Wed Sep  3 11:01:55 2014
From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay)
Date: Wed Sep  3 11:02:09 2014
Subject: [Histonet] "Standard" for UV irradiation of safety glasses?
Message-ID: <CAGBdDuDpJ5fZoNQwFPUeohAF_kRA0Wzt-pYVL3ZRJ6sxnzKepQ@mail.gmail.com>

Hello Histonetters:

Could someone tell me offline (blayjorge@gmail.com) what is the "standard"
(or general practice) for UV irradiation of safety glasses?

When I was heavily involved in molecular work, our lab used to leave PCR
tools (last person turned UV on, next person - sometimes overnight).
However, those were PCR tools, these are safety glasses used in
microbiological content.

Guidance welcomed (blayjorge@gmail.com).

Gratefully,

Jorge

Jorge A. Santiago-Blay, PhD
blaypublishers.com
http://blayjorge.wordpress.com/
http://paleobiology.si.edu/staff/individuals/santiagoblay.html
From amylee779 <@t> yahoo.com  Wed Sep  3 12:35:48 2014
From: amylee779 <@t> yahoo.com (Amy Lee)
Date: Wed Sep  3 12:39:39 2014
Subject: [Histonet] GPT2/ALT2 antibody
Message-ID: <1409765748.58761.YahooMailNeo@web126003.mail.ne1.yahoo.com>

Hello histonet,
 
Has anyone done GPT2/ALT2 IHC? Could you recommend an antibody works well on paraffin rat section?
I searched around but don't know which one is good. 
 
Thank you,
 
Amy
From mbplab <@t> yahoo.com  Wed Sep  3 15:17:01 2014
From: mbplab <@t> yahoo.com (Mary Benoit)
Date: Wed Sep  3 15:26:52 2014
Subject: [Histonet] high complexity testing
Message-ID: <1409775421.79091.YahooMailNeo@web161605.mail.bf1.yahoo.com>

What is the general opinion of the level of complexity for the Mohs tech assisting in a procedure.  This includes the embedding, cutting and staining of the samples only.  NOt the inking and marking of margins.  Thank you
Mary F Benoit, MT(ASCP)
The Pathology Laboratory
Lake Charles, LA 
From joelleweaver <@t> hotmail.com  Wed Sep  3 15:33:47 2014
From: joelleweaver <@t> hotmail.com (Joelle Weaver)
Date: Wed Sep  3 15:33:52 2014
Subject: [Histonet] QIHC test
In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011252B3A8@JERRY.Gia.com>
References: <24C4B3C167E5694887AB594C7602CE3A0D0D62@WN35104.or.providence.org>,
	<5A33C952BB67F4468AF1F36D739212BC011252B3A8@JERRY.Gia.com>
Message-ID: <SNT149-W858FD8914734D01AAD8474D8C40@phx.gbl>

Dako education guides, available from their website. Not "answer books", but good for reviewing theory. If the MSH one is like their other study guides, the learning activity is to look up and fill in the answers from other resources materials.


Joelle Weaver MAOM, HTL (ASCP) QIHC

        
  

 
> From: amber.mckenzie@gastrodocs.net
> To: histonet@lists.utsouthwestern.edu
> Date: Wed, 3 Sep 2014 14:26:52 +0000
> Subject: [Histonet] QIHC test
> 
> I ordered the MSH online study guide for the QIHC, but it was only questions...no answer key in the back.  What books am I supposed to get the answers from?  Any other study suggestions? Thanks!
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From raj <@t> bluemarble.net  Wed Sep  3 21:01:51 2014
From: raj <@t> bluemarble.net (Rebecca a. Johnson)
Date: Wed Sep  3 21:02:04 2014
Subject: [Histonet] New email address
Message-ID: <6AB01C68F86D42C5AAE7D31FE30878F8@BeckyPC>

Please change my email to histotech1948@gmail.com.


Thank You 
Rebecca A. Johnson
From brendal.finlay <@t> medicalcenterclinic.com  Thu Sep  4 05:23:14 2014
From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay)
Date: Thu Sep  4 05:23:55 2014
Subject: [Histonet] QIHC test
References: <24C4B3C167E5694887AB594C7602CE3A0D0D62@WN35104.or.providence.org>,
	<5A33C952BB67F4468AF1F36D739212BC011252B3A8@JERRY.Gia.com>
Message-ID: <8e9423a2376d72f92b39e5a40f3e5de5@medicalcenterclinic.com>

The one I received last year has essential and recommended reading lists on page 8.? I am still studying for the qualification.? I've been using the two guides from Dako, Carson's Histotechnology A Self-Instructional Text, and the elusive Elias' Immunohistopathology A Practical Approach to Diagnosis.? There are some Journal of Histotechnology articles I'd like to get my hands on, but have not been able to yet.? I'm also curious about the other books.


Brendal C. Finlay, HT (ASCP)
?
-----Original Message----- 
From: "Joelle Weaver" <joelleweaver@hotmail.com> 
To: "Amber McKenzie" <amber.mckenzie@gastrodocs.net>, histonet@lists.utsouthwestern.edu 
Date: 09/03/2014 15:33 
Subject: RE: [Histonet] QIHC test 

Dako education guides, available from their website. Not "answer books", but good for reviewing theory. If the MSH one is like their other study guides, the learning activity is to look up and fill in the answers from other resources materials.


Joelle Weaver MAOM, HTL (ASCP) QIHC

? ? ? ?
?


> From: amber.mckenzie@gastrodocs.net
> To: histonet@lists.utsouthwestern.edu
> Date: Wed, 3 Sep 2014 14:26:52 +0000
> Subject: [Histonet] QIHC test
> 
> I ordered the MSH online study guide for the QIHC, but it was only questions...no answer key in the back. ?What books am I supposed to get the answers from? ?Any other study suggestions? Thanks!
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
?????????? ????? ? ?????????? ?_______________________________________________
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From Bonnie.Whitaker <@t> osumc.edu  Thu Sep  4 06:22:05 2014
From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie)
Date: Thu Sep  4 06:25:51 2014
Subject: [Histonet] QIHC test
In-Reply-To: <8e9423a2376d72f92b39e5a40f3e5de5@medicalcenterclinic.com>
References: <24C4B3C167E5694887AB594C7602CE3A0D0D62@WN35104.or.providence.org>,
	<5A33C952BB67F4468AF1F36D739212BC011252B3A8@JERRY.Gia.com>,
	<8e9423a2376d72f92b39e5a40f3e5de5@medicalcenterclinic.com>
Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670E01CED0CD@EXMBOX-VP05.OSUMC.EDU>

The IHC Resource Committee hopes to soon have some IHC materials available via the NSH website.  I'll email the group as soon as I learn that it is active.  You will have to be an NSH member in order to have access to the links.

Thanks,
Bonnie Whitaker
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brendal Finlay [brendal.finlay@medicalcenterclinic.com]
Sent: Thursday, September 04, 2014 6:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re[2]: [Histonet] QIHC test

The one I received last year has essential and recommended reading lists on page 8.  I am still studying for the qualification.  I've been using the two guides from Dako, Carson's Histotechnology A Self-Instructional Text, and the elusive Elias' Immunohistopathology A Practical Approach to Diagnosis.  There are some Journal of Histotechnology articles I'd like to get my hands on, but have not been able to yet.  I'm also curious about the other books.


Brendal C. Finlay, HT (ASCP)

-----Original Message-----
From: "Joelle Weaver" <joelleweaver@hotmail.com>
To: "Amber McKenzie" <amber.mckenzie@gastrodocs.net>, histonet@lists.utsouthwestern.edu
Date: 09/03/2014 15:33
Subject: RE: [Histonet] QIHC test

Dako education guides, available from their website. Not "answer books", but good for reviewing theory. If the MSH one is like their other study guides, the learning activity is to look up and fill in the answers from other resources materials.


Joelle Weaver MAOM, HTL (ASCP) QIHC





> From: amber.mckenzie@gastrodocs.net
> To: histonet@lists.utsouthwestern.edu
> Date: Wed, 3 Sep 2014 14:26:52 +0000
> Subject: [Histonet] QIHC test
>
> I ordered the MSH online study guide for the QIHC, but it was only questions...no answer key in the back.  What books am I supposed to get the answers from?  Any other study suggestions? Thanks!
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=ux7ohqYFcw1oDo0gOpSLlw%3D%3D%0A&r=WjFHCxww2Yu7mXS0AG%2B%2FG6AKSvvnC5BJWJTOtfrZAx8%3D%0A&m=jbCeVcITp0Y2nEVwIovDtsu%2F73hEFPGHkHk2UbkjkQk%3D%0A&s=7eb46b17bdcf773df6998a7a0bad7c35decaafd60599fdec4877b0334f467b1b
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From amber.mckenzie <@t> gastrodocs.net  Thu Sep  4 08:23:50 2014
From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie)
Date: Thu Sep  4 08:23:55 2014
Subject: [Histonet] No food or drink in the lab
In-Reply-To: <178336186.4130836.1409681334364.JavaMail.root@comcast.net>
References: <1BA185E7-E5D4-4B0D-8CCC-978212072356@deltacollege.edu>
	<761E2B5697F795489C8710BCC72141FF36796206@ex07.net.ucsf.edu>
	<178336186.4130836.1409681334364.JavaMail.root@comcast.net>
Message-ID: <5A33C952BB67F4468AF1F36D739212BC011252B988@JERRY.Gia.com>

Does anyone know of a specific site and quote that states "No food or drink in the lab" and what the consequences would be?  That covers drinks/snacks in the cabinets above their workstations too, right? I have a sign up on the door entering the lab that states no food or drink, but the staff continue to keep stuff around their desk, so I need something to back me up from CLIA, OSHA, CAP, etc...

Thanks!


From mpence <@t> grhs.net  Thu Sep  4 08:36:17 2014
From: mpence <@t> grhs.net (Mike Pence)
Date: Thu Sep  4 08:36:37 2014
Subject: [Histonet] RE: No food or drink in the lab
In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011252B988@JERRY.Gia.com>
References: <1BA185E7-E5D4-4B0D-8CCC-978212072356@deltacollege.edu>
	<761E2B5697F795489C8710BCC72141FF36796206@ex07.net.ucsf.edu>
	<178336186.4130836.1409681334364.JavaMail.root@comcast.net>
	<5A33C952BB67F4468AF1F36D739212BC011252B988@JERRY.Gia.com>
Message-ID: <D6DF96C438FB8D42B04B2345A9452CF501633B3DCF@MAIL-MB02.grhs.net>

For one discipline. It is a OSHA, Joint Commission and CAP standards. I do know it is a phase II for CAP. It is also a hospital policy at my place. 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Thursday, September 04, 2014 8:26 AM
Cc: Histonet
Subject: [Histonet] No food or drink in the lab

Does anyone know of a specific site and quote that states "No food or drink in the lab" and what the consequences would be?  That covers drinks/snacks in the cabinets above their workstations too, right? I have a sign up on the door entering the lab that states no food or drink, but the staff continue to keep stuff around their desk, so I need something to back me up from CLIA, OSHA, CAP, etc...

Thanks!


From Terra.Wineman <@t> novusint.com  Thu Sep  4 08:38:50 2014
From: Terra.Wineman <@t> novusint.com (Wineman, Terra)
Date: Thu Sep  4 08:38:56 2014
Subject: [Histonet] RE: No food or drink in the lab
In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011252B988@JERRY.Gia.com>
References: <1BA185E7-E5D4-4B0D-8CCC-978212072356@deltacollege.edu>
	<761E2B5697F795489C8710BCC72141FF36796206@ex07.net.ucsf.edu>
	<178336186.4130836.1409681334364.JavaMail.root@comcast.net>
	<5A33C952BB67F4468AF1F36D739212BC011252B988@JERRY.Gia.com>
Message-ID: <1EB8F245A303564EADF12AC7022FA74DCB33BA9B@novus-ex01>

Pretty sure that is an OSHA regulation.

Terra Wineman, HTL (ASCP)CM
Research Biologist, Nutritional Physiology
636-926-7476 phone
terra.wineman@novusint.com


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Thursday, September 04, 2014 8:24 AM
Cc: Histonet
Subject: [Histonet] No food or drink in the lab

Does anyone know of a specific site and quote that states "No food or drink in the lab" and what the consequences would be?  That covers drinks/snacks in the cabinets above their workstations too, right? I have a sign up on the door entering the lab that states no food or drink, but the staff continue to keep stuff around their desk, so I need something to back me up from CLIA, OSHA, CAP, etc...

Thanks!


From PAMarcum <@t> uams.edu  Thu Sep  4 08:52:54 2014
From: PAMarcum <@t> uams.edu (Marcum, Pamela A)
Date: Thu Sep  4 08:53:01 2014
Subject: [Histonet] RE: No food or drink in the lab
In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011252B988@JERRY.Gia.com>
References: <1BA185E7-E5D4-4B0D-8CCC-978212072356@deltacollege.edu>
	<761E2B5697F795489C8710BCC72141FF36796206@ex07.net.ucsf.edu>
	<178336186.4130836.1409681334364.JavaMail.root@comcast.net>
	<5A33C952BB67F4468AF1F36D739212BC011252B988@JERRY.Gia.com>
Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3201252666F8@Mail2Node2.ad.uams.edu>

I have not worked in or visited as technical representative since the middle 70' that allowed in any food or drink in the lab anywhere.  If you have food or snacks they go in your locker and out of the lab or don't bring them in.  

In most labs this an automatic write up and can lead to progressive disciplinary action.  Your safety officer or department head should be all over this and if you don't have one check CLIA, CAP and any other letter organization to find it.  This is a health and safety issue that is taken seriously everywhere.  

If I find someone with food or drink in this lab they are disciplined before one of our safety officers from OHS finds out.  The fact that it is hidden or in cabinet does not cut it - No food or drink in the lab period. 

Pam Marcum
UAMS

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Thursday, September 04, 2014 8:24 AM
Cc: Histonet
Subject: [Histonet] No food or drink in the lab

Does anyone know of a specific site and quote that states "No food or drink in the lab" and what the consequences would be?  That covers drinks/snacks in the cabinets above their workstations too, right? I have a sign up on the door entering the lab that states no food or drink, but the staff continue to keep stuff around their desk, so I need something to back me up from CLIA, OSHA, CAP, etc...

Thanks!



----------------------------------------------------------------------
Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
From oskaki <@t> morpheus.wustl.edu  Thu Sep  4 08:54:57 2014
From: oskaki <@t> morpheus.wustl.edu (Osborne, Dale)
Date: Thu Sep  4 08:55:02 2014
Subject: [Histonet] RE: No food or drink in the lab
In-Reply-To: <1EB8F245A303564EADF12AC7022FA74DCB33BA9B@novus-ex01>
References: <1BA185E7-E5D4-4B0D-8CCC-978212072356@deltacollege.edu>
	<761E2B5697F795489C8710BCC72141FF36796206@ex07.net.ucsf.edu>
	<178336186.4130836.1409681334364.JavaMail.root@comcast.net>
	<5A33C952BB67F4468AF1F36D739212BC011252B988@JERRY.Gia.com>,
	<1EB8F245A303564EADF12AC7022FA74DCB33BA9B@novus-ex01>
Message-ID: <FC0D08A1CDE12F4B972D1F33E2184B983998A1C9@CITEMB1A.cits.wustl.edu>

Hello,
   I've attached the policy that Washington University has regarding food and drink in the lab.  If found upon lab inspection, the university and/or department may be fined.

Dale Osborne
Washington University School of Medicine
Anesthesiology Dept.
St. Louis, MO
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Wineman, Terra [Terra.Wineman@novusint.com]
Sent: Thursday, September 04, 2014 8:38 AM
To: Amber McKenzie
Cc: Histonet
Subject: [Histonet] RE: No food or drink in the lab

Pretty sure that is an OSHA regulation.

Terra Wineman, HTL (ASCP)CM
Research Biologist, Nutritional Physiology
636-926-7476 phone
terra.wineman@novusint.com


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Thursday, September 04, 2014 8:24 AM
Cc: Histonet
Subject: [Histonet] No food or drink in the lab

Does anyone know of a specific site and quote that states "No food or drink in the lab" and what the consequences would be?  That covers drinks/snacks in the cabinets above their workstations too, right? I have a sign up on the door entering the lab that states no food or drink, but the staff continue to keep stuff around their desk, so I need something to back me up from CLIA, OSHA, CAP, etc...

Thanks!



________________________________
The materials in this message are private and may contain Protected Healthcare Information or other information of a sensitive nature. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail.
From j.rowaihi <@t> alborglaboratories.com  Thu Sep  4 08:59:24 2014
From: j.rowaihi <@t> alborglaboratories.com (Jamal)
Date: Thu Sep  4 08:56:34 2014
Subject: [Histonet] No food or drink in the lab
In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011252B988@JERRY.Gia.com>
References: <1BA185E7-E5D4-4B0D-8CCC-978212072356@deltacollege.edu>	<761E2B5697F795489C8710BCC72141FF36796206@ex07.net.ucsf.edu>	<178336186.4130836.1409681334364.JavaMail.root@comcast.net>
	<5A33C952BB67F4468AF1F36D739212BC011252B988@JERRY.Gia.com>
Message-ID: <042001cfc848$707c0450$51740cf0$@rowaihi@alborglaboratories.com>

In our laboratory this matter are controlled by the safety department which give safety orientation for every new staff which include instruction about handling the food and drinks in the working areas and the disciplinary action which will be done in case of occurs by staff, and the staff will sign acknowledge on that orientation.


Best Regards,


Jamal M. Al Rowaihi	Anatomic Pathology Supervisor       | Al Borg Medical Laboratories |  Mobile +966 503629832| j.rowaihi@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA    | Phone: +966 12 670 0099	      | Fax: +966 12 676 4984     | www.alborglaboratories.com


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Thursday, September 04, 2014 4:24 PM
Cc: Histonet
Subject: [Histonet] No food or drink in the lab

Does anyone know of a specific site and quote that states "No food or drink in the lab" and what the consequences would be?  That covers drinks/snacks in the cabinets above their workstations too, right? I have a sign up on the door entering the lab that states no food or drink, but the staff continue to keep stuff around their desk, so I need something to back me up from CLIA, OSHA, CAP, etc...

Thanks!




From ahorvath <@t> cogipath.com  Thu Sep  4 09:02:43 2014
From: ahorvath <@t> cogipath.com (Andrew Horvath)
Date: Thu Sep  4 09:02:14 2014
Subject: [Histonet] No food or drink in the lab
In-Reply-To: <042001cfc848$707c0450$51740cf0$@rowaihi@alborglaboratories.com>
References: <1BA185E7-E5D4-4B0D-8CCC-978212072356@deltacollege.edu>	<761E2B5697F795489C8710BCC72141FF36796206@ex07.net.ucsf.edu>	<178336186.4130836.1409681334364.JavaMail.root@comcast.net>	<5A33C952BB67F4468AF1F36D739212BC011252B988@JERRY.Gia.com>
	<042001cfc848$707c0450$51740cf0$@rowaihi@alborglaboratories.com>
Message-ID: <003501cfc848$e5896120$b09c2360$@cogipath.com>

Here is the CAP checklist item, checklist date 4.21.14



GEN.74400	Eating/Mouth Pipetting	Phase II
 	There is a written policy that prohibits smoking, eating, drinking, application of cosmetics and lip balm, manipulation of contact lenses, and mouth pipetting in all technical work areas.
 


These were listed on the prior checklist from 9.25.12

REFERENCES
1) 	NCCLS. Clinical Laboratory Safety; Approved Guideline?Second Edition. NCCLS document GP17-A2 (ISBN 1-56238-530-5). NCCLS, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087-1898 USA, 2004 
2) 	Occupational Safety and Health Administration. Toxic and hazardous substances. Bloodborne pathogens. Washington, DC: US Government Printing Office, 1999(Jul 1): [29CFR1910.1030] 





-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jamal
Sent: Thursday, September 04, 2014 7:59 AM
To: 'Amber McKenzie'
Cc: 'Histonet'
Subject: RE: [Histonet] No food or drink in the lab

In our laboratory this matter are controlled by the safety department which give safety orientation for every new staff which include instruction about handling the food and drinks in the working areas and the disciplinary action which will be done in case of occurs by staff, and the staff will sign acknowledge on that orientation.


Best Regards,


Jamal M. Al Rowaihi	Anatomic Pathology Supervisor       | Al Borg Medical Laboratories |  Mobile +966 503629832| j.rowaihi@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA    | Phone: +966 12 670 0099	      | Fax: +966 12 676 4984     | www.alborglaboratories.com


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Thursday, September 04, 2014 4:24 PM
Cc: Histonet
Subject: [Histonet] No food or drink in the lab

Does anyone know of a specific site and quote that states "No food or drink in the lab" and what the consequences would be?  That covers drinks/snacks in the cabinets above their workstations too, right? I have a sign up on the door entering the lab that states no food or drink, but the staff continue to keep stuff around their desk, so I need something to back me up from CLIA, OSHA, CAP, etc...

Thanks!




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From Toni.Rathborne <@t> rwjuh.edu  Thu Sep  4 09:03:23 2014
From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni)
Date: Thu Sep  4 09:04:15 2014
Subject: [Histonet] RE: No food or drink in the lab
In-Reply-To: <FC0D08A1CDE12F4B972D1F33E2184B983998A1C9@CITEMB1A.cits.wustl.edu>
References: <1BA185E7-E5D4-4B0D-8CCC-978212072356@deltacollege.edu>
	<761E2B5697F795489C8710BCC72141FF36796206@ex07.net.ucsf.edu>
	<178336186.4130836.1409681334364.JavaMail.root@comcast.net>
	<5A33C952BB67F4468AF1F36D739212BC011252B988@JERRY.Gia.com>,
	<1EB8F245A303564EADF12AC7022FA74DCB33BA9B@novus-ex01>
	<FC0D08A1CDE12F4B972D1F33E2184B983998A1C9@CITEMB1A.cits.wustl.edu>
Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24EC6FC0@vap1014.win.rwjuh.edu>

What is staff doing at their desks? Are the desks located in a work area where specimens or chemicals are? If not, and the desk area can be  clearly separated from the work area, then maybe  you could have a sign posted in each area. "No food or drink permitted in this area" or "Food and drink permitted in this area".  This way it clearly states where the food will be allowed. If their desks are in an area where specimens are handled, then disciplinary action should follow.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Osborne, Dale
Sent: Thursday, September 04, 2014 9:55 AM
To: Wineman, Terra; Amber McKenzie
Cc: Histonet
Subject: [Histonet] RE: No food or drink in the lab

Hello,
   I've attached the policy that Washington University has regarding food and drink in the lab.  If found upon lab inspection, the university and/or department may be fined.

Dale Osborne
Washington University School of Medicine Anesthesiology Dept.
St. Louis, MO
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Wineman, Terra [Terra.Wineman@novusint.com]
Sent: Thursday, September 04, 2014 8:38 AM
To: Amber McKenzie
Cc: Histonet
Subject: [Histonet] RE: No food or drink in the lab

Pretty sure that is an OSHA regulation.

Terra Wineman, HTL (ASCP)CM
Research Biologist, Nutritional Physiology
636-926-7476 phone
terra.wineman@novusint.com


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Thursday, September 04, 2014 8:24 AM
Cc: Histonet
Subject: [Histonet] No food or drink in the lab

Does anyone know of a specific site and quote that states "No food or drink in the lab" and what the consequences would be?  That covers drinks/snacks in the cabinets above their workstations too, right? I have a sign up on the door entering the lab that states no food or drink, but the staff continue to keep stuff around their desk, so I need something to back me up from CLIA, OSHA, CAP, etc...

Thanks!



________________________________
The materials in this message are private and may contain Protected Healthcare Information or other information of a sensitive nature. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail.


From areichard <@t> lmhealth.org  Thu Sep  4 09:33:28 2014
From: areichard <@t> lmhealth.org (Amanda Reichard)
Date: Thu Sep  4 09:33:43 2014
Subject: [Histonet] Radioactive specimens policy
Message-ID: <E9A90E28259D2F4E84308C5E8EA8F7B401A342688819@lmhs-exchange>

Good morning everyone!

Would anyone be willing to share their policy/procedure for radioactive specimen acceptance, transport, storage, and disposal?
We are currently revising our policy and would like to see what precautions, if any, other institutions establish in the laboratory.

Thanks in advance!

Amanda Reichard, HTL (ASCP)cm
Histotechnologist
Laboratory
Licking Memorial Health Systems
1320 W. Main St.
Newark, OH 43055
(740) 348-4157

________________________________
This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.

From PAMarcum <@t> uams.edu  Thu Sep  4 10:52:19 2014
From: PAMarcum <@t> uams.edu (Marcum, Pamela A)
Date: Thu Sep  4 10:52:31 2014
Subject: [Histonet] Job Opportunity HT or HTL at UAMS in Little Rock AR
Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32012526677D@Mail2Node2.ad.uams.edu>

We have a job opening for an ASCP registered HT or HTL in our active and modern Histology Department.  We are connected with one of the largest Multiple Myeloma Departments with numerous bone marrows per day as well as biopsies and routine surgicals.  We do routine staining and have automated our special stains with a few exceptions.  The person applying should be motivated and be able to see and pick up on whatever we need done during the day.  The shift will be 6AM to 2:30PM daily with one weekend every four to five weeks on call for Saturday and RUSH case (these are rare).  We are still growing and looking forward to a move soon and expansion. (IHC is done in another area.)

UAMS offers some great benefits and opportunities to go back to school with very generous discounts.  Arkansas is great place for outdoor activities and beautiful surroundings.   If you are interested please contact me by sending a resume and/or phone number so I can reach you to discuss your experience or you can ask me questions about the position.

Please recruiters: we are not able to use you for placements so do not answer this.  We cannot by state regulations use you for this position or in most areas of the hospital.


Best Regards,

Pamela A Marcum
Supervisor AP Hisotlogy
University of Arkansas for Medical Sciences
4301 W Markham Street
Little Rock AR 72205

----------------------------------------------------------------------
Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
From rsrichmond <@t> gmail.com  Thu Sep  4 12:16:28 2014
From: rsrichmond <@t> gmail.com (Bob Richmond)
Date: Thu Sep  4 12:16:31 2014
Subject: [Histonet] Re: Radioactive specimens policy
Message-ID: <CAOKsRH5PL1_9kT9jKp9QF2jz+=a0+5QLJZAHKmN+RpGJqp_gnQ@mail.gmail.com>

Amanda Reichard, HTL (ASCP)cm, at Licking Memorial Health Systems in
Newark, Ohio asks:

>>Would anyone be willing to share their policy/procedure for radioactive
specimen acceptance, transport, storage, and disposal? - We are currently
revising our policy and would like to see what precautions, if any, other
institutions establish in the laboratory.<<

I've never seen a written policy - these questions are customarily swept
under the rug - but I've seen references though I have no very current ones.

By far the most common specimens are breast masses and sentinel lymph nodes
with technetium 99m, which has a half-life of only 6 hours. These specimens
don't require any special handling beyond Universal Precautions.

I haven't been able to get a lot of information about the radioactive
"seeds" used to treat prostate cancer, and occasionally received in TURP
specimens. The isotopes used have half-lives of around 70 days, so they
would be regarded as being potentially hazardous for around two years (ten
half-lives). It usually takes a phone call to find out how long ago the
"seeds" were put in.

Bob Richmond
Samurai Pathologist
Maryville TN
From jerrysedgewick <@t> gmail.com  Thu Sep  4 12:52:30 2014
From: jerrysedgewick <@t> gmail.com (jerry sedgewick)
Date: Thu Sep  4 12:52:46 2014
Subject: [Histonet] Color in Sections with Adipose Tissue?
Message-ID: <5408A6DE.3090308@gmail.com>

I've observed images on a computer screen with a person next to me 
insisting that the adipose tissue in H&E stained tissue has a color 
versus areas with no tissue (open areas).  Am I missing something, or do 
others see either a slight discoloration in adipose tissue, or a 
slightly different color, versus areas without tissue?  I suspect it 
depends on section thickness, but given a 5 - 8 micron section it seems 
that whatever tissue might have been present would be scraped away with 
the blade.

Jerry Sedgewick
From jerrysedgewick <@t> gmail.com  Thu Sep  4 13:59:06 2014
From: jerrysedgewick <@t> gmail.com (jerry sedgewick)
Date: Thu Sep  4 13:59:17 2014
Subject: [Histonet] Color in Sections with Adipose Tissue?
In-Reply-To: <1878897195.918519.1409853757450.JavaMail.root@comcast.net>
References: <5408A6DE.3090308@gmail.com>
	<1878897195.918519.1409853757450.JavaMail.root@comcast.net>
Message-ID: <5408B67A.4000402@gmail.com>

Oh yeah, didn't think about frozen vs paraffin section since I'm an 
imaging guy.  If it were frozen, would some remnant of the adipose 
tissue remain after sectioning? Thanks!

On 9/4/2014 1:02 PM, jsjurczak@comcast.net wrote:
>
> Are you talking frozens here?
>
>
>
> ------------------------------------------------------------------------
> *From: *"jerry sedgewick" <jerrysedgewick@gmail.com>
> *To: *"Histonet@lists.utsouthwestern.edu" 
> <histonet@lists.utsouthwestern.edu>
> *Sent: *Thursday, September 4, 2014 12:52:30 PM
> *Subject: *[Histonet] Color in Sections with Adipose Tissue?
>
> I've observed images on a computer screen with a person next to me
> insisting that the adipose tissue in H&E stained tissue has a color
> versus areas with no tissue (open areas).  Am I missing something, or do
> others see either a slight discoloration in adipose tissue, or a
> slightly different color, versus areas without tissue?  I suspect it
> depends on section thickness, but given a 5 - 8 micron section it seems
> that whatever tissue might have been present would be scraped away with
> the blade.
>
> Jerry Sedgewick
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

From jerrysedgewick <@t> gmail.com  Thu Sep  4 14:20:26 2014
From: jerrysedgewick <@t> gmail.com (jerry sedgewick)
Date: Thu Sep  4 14:20:36 2014
Subject: [Histonet] Color in Sections with Adipose Tissue?
In-Reply-To: <CAGW4+=ojY2mKSKOc7g=7=MjJyARMsvVHe7Lqsjrfwejki-hXCg@mail.gmail.com>
References: <5408A6DE.3090308@gmail.com>
	<CAGW4+=ojY2mKSKOc7g=7=MjJyARMsvVHe7Lqsjrfwejki-hXCg@mail.gmail.com>
Message-ID: <5408BB7A.9020803@gmail.com>

Thanks, Colleen...Got it!


On 9/4/2014 1:55 PM, Colleen Forster wrote:
> When I cut adipose tissues Jerry, that have been fixed and processed 
> you will get a slide that appears clear or like tissue is missing in 
> between the vessels. If you look under the scope you will see fine 
> cell membranes that outline the fat cell. The fat dissolves during 
> processing leaving only the membrane and vasculature. If they want to 
> see the actual fat it has to be done by frozen section.
>
> Does this make sense?
>
> C
>
>
> On Thu, Sep 4, 2014 at 12:52 PM, jerry sedgewick 
> <jerrysedgewick@gmail.com <mailto:jerrysedgewick@gmail.com>> wrote:
>
>     I've observed images on a computer screen with a person next to me
>     insisting that the adipose tissue in H&E stained tissue has a
>     color versus areas with no tissue (open areas).  Am I missing
>     something, or do others see either a slight discoloration in
>     adipose tissue, or a slightly different color, versus areas
>     without tissue?  I suspect it depends on section thickness, but
>     given a 5 - 8 micron section it seems that whatever tissue might
>     have been present would be scraped away with the blade.
>
>     Jerry Sedgewick
>     _______________________________________________
>     Histonet mailing list
>     Histonet@lists.utsouthwestern.edu
>     <mailto:Histonet@lists.utsouthwestern.edu>
>     http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>

From amylee779 <@t> yahoo.com  Thu Sep  4 16:25:55 2014
From: amylee779 <@t> yahoo.com (Amy Lee)
Date: Thu Sep  4 16:25:59 2014
Subject: [Histonet] GPT2/ALT2 antibody
Message-ID: <1409865955.20031.YahooMailNeo@web126005.mail.ne1.yahoo.com>

Hello histonet,
It seems my last sent out email got lost. So I have to send it again. Could anyone recommend an GPT2/ALT2 antibody works on rat paraffin section please? I did some search but don't know which one is good.
 
Thanks,
 
Amy Lee
From joelleweaver <@t> hotmail.com  Fri Sep  5 07:16:21 2014
From: joelleweaver <@t> hotmail.com (Joelle Weaver)
Date: Fri Sep  5 07:16:26 2014
Subject: [Histonet] Re: Radioactive specimens policy
In-Reply-To: <CAOKsRH5PL1_9kT9jKp9QF2jz+=a0+5QLJZAHKmN+RpGJqp_gnQ@mail.gmail.com>
References: <CAOKsRH5PL1_9kT9jKp9QF2jz+=a0+5QLJZAHKmN+RpGJqp_gnQ@mail.gmail.com>
Message-ID: <SNT149-W246B8B8DD5A43A04B6BF41D8C20@phx.gbl>

Had CAP inspection yesterday, while this was not specifically raised as an issue, my pathologist advised me to address in policy even though it is not terribly applicable in this lab situation. I was able to include with the exclusion list, specifically addressing the seeds and breast masses, sentinel lymph nodes, and this works with this being a reference facility that has no attached surgical facilities and so already has limits on the specimen types accepted for testing. This most likely would not suffice for a hospital situation. So short answer, I put a "policy statement together" within another policy, but a free standing policy might be needed depending on how much you see/handle these types of specimens.  Hope this helps.


Joelle Weaver MAOM, HTL (ASCP) QIHC

        
  

 
> Date: Thu, 4 Sep 2014 13:16:28 -0400
> From: rsrichmond@gmail.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Re: Radioactive specimens policy
> 
> Amanda Reichard, HTL (ASCP)cm, at Licking Memorial Health Systems in
> Newark, Ohio asks:
> 
> >>Would anyone be willing to share their policy/procedure for radioactive
> specimen acceptance, transport, storage, and disposal? - We are currently
> revising our policy and would like to see what precautions, if any, other
> institutions establish in the laboratory.<<
> 
> I've never seen a written policy - these questions are customarily swept
> under the rug - but I've seen references though I have no very current ones.
> 
> By far the most common specimens are breast masses and sentinel lymph nodes
> with technetium 99m, which has a half-life of only 6 hours. These specimens
> don't require any special handling beyond Universal Precautions.
> 
> I haven't been able to get a lot of information about the radioactive
> "seeds" used to treat prostate cancer, and occasionally received in TURP
> specimens. The isotopes used have half-lives of around 70 days, so they
> would be regarded as being potentially hazardous for around two years (ten
> half-lives). It usually takes a phone call to find out how long ago the
> "seeds" were put in.
> 
> Bob Richmond
> Samurai Pathologist
> Maryville TN
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From cjbulmer <@t> sbcglobal.net  Fri Sep  5 09:54:28 2014
From: cjbulmer <@t> sbcglobal.net (Cindy Bulmer)
Date: Fri Sep  5 09:54:32 2014
Subject: [Histonet] HercepTest Kit K5207
Message-ID: <1409928868.22393.YahooMailNeo@web180905.mail.ne1.yahoo.com>

 Hello Histoland,

Dako is experiencing a shortage of the FDA HercepTest kits, K5207, hopefully they will get more in the end of Sept.
I am in panic mode, as I only have enough stock to stain patients through next week!
Does anyone have enough stock to loan me a kit????  Our company will pay for shipping and certainly replace the kit
the moment Dako receives theirs.

Thank you,
Cindy

Cynthia Bulmer HT(ASCP)QIHC 
IHC Supervisor, CTPL 
Waco, TX
From plucas <@t> biopath.org  Fri Sep  5 12:12:46 2014
From: plucas <@t> biopath.org (Paula Lucas)
Date: Fri Sep  5 12:06:08 2014
Subject: [Histonet] 
	Histotech Job Opportunity/Fountain Valley, Orange County, CA
Message-ID: <41B1ADD066AA4D4480B6A060A21A2585@biopath.local>

Please no hiring staff services calling.

 

We are in need of a part-time histotechnician who can work on Tuesdays and
Saturdays, and then also to fill in during the week when needed.

 

Hours start at 5 am. We work Tuesdays through Saturdays.

 

Only experienced histotechs apply, which means previous experience in a lab
work environment (not as an intern), and being able to cut (microtomy), and
embed all types of tissues.  

 

We are a busy lab who supports hospitals, surgery centers and physician
practice offices

Please send resume to:

Paula Lucas

714-755-2984.

Thank you

 

 

From craigak12 <@t> gmail.com  Fri Sep  5 13:11:11 2014
From: craigak12 <@t> gmail.com (Jb)
Date: Fri Sep  5 13:11:19 2014
Subject: [Histonet] Tissue Processor Validation:
Message-ID: <BFC828D7-A0EC-418B-AF13-7F65D06DE227@gmail.com>

Does anyone have a tissue processor validation form that they are willing to share?

Thank you. 

Sent from my iPhone
From tbraud <@t> holyredeemer.com  Fri Sep  5 13:24:19 2014
From: tbraud <@t> holyredeemer.com (Terri  Braud)
Date: Fri Sep  5 13:24:24 2014
Subject: [Histonet] RE: Radioactive Policies
In-Reply-To: <20140905160816.B436D1E80A1@trendmess-svr.holyredeemer.local>
References: <20140905160816.B436D1E80A1@trendmess-svr.holyredeemer.local>
Message-ID: <BFBF7C6B9627B947B74CBEBD45CE368B142BF0EC@hrex-svr.holyredeemer.local>

Hi -  I Think I can help.  We have a policy and I've provided it here.
Also, we have a separate policy in our autopsy manual concerning the
handling of radioactive cadavers, which is rather extensive.  We also
participate in an annual Radiation Accident Drill which is county wide
and covers emergency response teams, hospital emergency, laboratory, and
morgue services.  Its quite a big deal to witness and lots of fun to
participate in.
Here is our tissue procedure.  Please let me know if you need a copy of
the other.

PURPOSE: To provide guidelines for the handling of sentinel lymph nodes
and other tissue with possible radioactivity.

PRINCIPLE:
The technique of sentinel lymph node biopsy (SLN) attempts to detect
regional node metastases by identification of the "sentinel" lymph
node(s) using a combination of radiolabeled colloid (99m Technetium
labeled sulfur or Human Serum Albumin) and isosulfuran blue dye.  The
premise is that lymph node metastases will initially travel by lymphatic
drainage to the "sentinel" node.  Other tissues that might contain
radioactive substances are identified and handled according to the
Radiation Safety Officer's recommendations.

PROCEDURE:

1.	During the validation phase of the SLN biopsy technique, the
sentinel lymph node(s) and the completion lymph node dissection will be
submitted to Pathology in separately labeled containers.  For mapping
with a radiolabeled colloid, an injected dose of technetium (99mTc) in
the range of 0.1 to 1.0 mCi (3.7 to 37 MBq) is approximately 4% of that
administered for a conventional bone scan. No isolation, precautions, or
special radiation monitoring are required. 

2.	Specimens should be submitted in 10% neutral buffered formalin
unless an intraoperative consultation is requested.

3.	Gross examination will identify the number and the size of the
sentinel lymph node(s).  Nodes should be sectioned into 2-3 mm slices.
Grossly evident metastatic disease should be noted. 

4.	Following tissue processing, at the pathologist's instruction,
the SLN block will be sectioned at 3 levels at 4 microns.  All levels
will be stained with hematoxylin and eosin.  Immunostaining will also be
performed when appropriate.  Lymph nodes demonstrating metastatic
disease on Frozen Section are routinely cut at one level only.

5.	If the pathologist does not ask for the protocol procedure, then
the lymph node is to be sectioned as regular tissue.

6.	For any other tissue sent to the Histology, suspected or labeled
as containing radioactive substances, the Histology Tech is to
immediately call the Hospital Radiation Safety Officer for radiation
measurement and instructions for the handling of the tissue. 

7.	The tech will immediately notify the pathologist on call that
tissue with suspect radiation has been received and the Radiation Safety
Officer has been called 

8.	The Radiation Safety Officer's handling instructions will
supersede any further processing of the tissue. Documentation of the
Radiation Safety Officer's tissue handling recommendations will be
included on the requisition.


Reference: JCO October 20, 2005 vol. 23 no. 30 7703-7720


Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874


-----Original Message-----

Today's Topics:
   1. Re: Radioactive specimens policy (Bob Richmond)
   6. RE: Re: Radioactive specimens policy (Joelle Weaver)
   

---------------------------------------------------------------------------------



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From j.rowaihi <@t> alborglaboratories.com  Sat Sep  6 04:34:10 2014
From: j.rowaihi <@t> alborglaboratories.com (Jamal)
Date: Sat Sep  6 04:31:25 2014
Subject: [Histonet] Tissue Processor Validation:
In-Reply-To: <BFC828D7-A0EC-418B-AF13-7F65D06DE227@gmail.com>
References: <BFC828D7-A0EC-418B-AF13-7F65D06DE227@gmail.com>
Message-ID: <002801cfc9b5$b813c280$283b4780$@rowaihi@alborglaboratories.com>

Hi Jb
The attached is my validation form you can refer to it.


Best Regards,


Jamal M. Al Rowaihi	Anatomic Pathology Supervisor       | Al Borg
Medical Laboratories |? Mobile +966 503629832|
j.rowaihi@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA    |
Phone: +966 12 670 0099	      | Fax: +966 12 676 4984     |
www.alborglaboratories.com


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Friday, September 05, 2014 9:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Validation:

Does anyone have a tissue processor validation form that they are willing to
share?

Thank you. 

Sent from my iPhone
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From udsd007 <@t> gmail.com  Sat Sep  6 10:04:45 2014
From: udsd007 <@t> gmail.com (Mike Andrews)
Date: Sat Sep  6 10:04:52 2014
Subject: [Histonet] Tissue Processor Validation:
In-Reply-To: <540ad49e.21f1b60a.2980.6d05SMTPIN_ADDED_BROKEN@mx.google.com>
References: <BFC828D7-A0EC-418B-AF13-7F65D06DE227@gmail.com>
	<540ad49e.21f1b60a.2980.6d05SMTPIN_ADDED_BROKEN@mx.google.com>
Message-ID: <356EC6D1-7CA6-408F-9F35-5E1BD6A0C62A@gmail.com>

It appears that the list software strips attachments. You may want to put it on a website and post the URL.

Mike Andrews, W5EGO
WWME Oklahoma area executive team

> On Sep 6, 2014, at 4:34 AM, "Jamal" <j.rowaihi@alborglaboratories.com> wrote:
> 
> Hi Jb
> The attached is my validation form you can refer to it.
> 
> 
> Best Regards,
> 
> 
> Jamal M. Al Rowaihi    Anatomic Pathology Supervisor       | Al Borg
> Medical Laboratories |  Mobile +966 503629832|
> j.rowaihi@alborglaboratories.com 
> Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA    |
> Phone: +966 12 670 0099          | Fax: +966 12 676 4984     |
> www.alborglaboratories.com
> 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb
> Sent: Friday, September 05, 2014 9:11 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Tissue Processor Validation:
> 
> Does anyone have a tissue processor validation form that they are willing to
> share?
> 
> Thank you. 
> 
> Sent from my iPhone
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From hans <@t> histologistics.com  Sat Sep  6 16:43:01 2014
From: hans <@t> histologistics.com (Hans B Snyder)
Date: Sat Sep  6 16:43:08 2014
Subject: [Histonet] Neutrophil staining
Message-ID: <CAAYBjct_WYuDuhi1DY9LtPp7+1uLnQ1_1SfbpK8bdB-3kZRN=g@mail.gmail.com>

Hello All,

I am looking to  stain neutrophils using special stains and antibodies
in mice tissue.  I wanted your opinion about the best methods to do
so.  I am thinking of using the may Grunwald giemsa and T-blue for
specials and myeloperoxidase and Ly6G for the antibodies.

What are your favorite special stains and antibodies to stain for neutrophils?

Best regards

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
hans@histologistics.com

From liz <@t> premierlab.com  Sat Sep  6 20:04:59 2014
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Sat Sep  6 20:08:17 2014
Subject: [Histonet] Neutrophil staining
In-Reply-To: <CAAYBjct_WYuDuhi1DY9LtPp7+1uLnQ1_1SfbpK8bdB-3kZRN=g@mail.gmail.com>
References: <CAAYBjct_WYuDuhi1DY9LtPp7+1uLnQ1_1SfbpK8bdB-3kZRN=g@mail.gmail.com>
Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E021DE@SBS2K8.premierlab.local>


Serotec has a rat anti-mouse neutrophil marker that works in FFPE tissues

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
liz@premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder [hans@histologistics.com]
Sent: Saturday, September 06, 2014 3:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Neutrophil staining

Hello All,

I am looking to  stain neutrophils using special stains and antibodies
in mice tissue.  I wanted your opinion about the best methods to do
so.  I am thinking of using the may Grunwald giemsa and T-blue for
specials and myeloperoxidase and Ly6G for the antibodies.

What are your favorite special stains and antibodies to stain for neutrophils?

Best regards

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
hans@histologistics.com

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From kaarrington <@t> anthc.org  Sun Sep  7 18:49:40 2014
From: kaarrington <@t> anthc.org (Arrington, Karla A)
Date: Sun Sep  7 18:49:46 2014
Subject: [Histonet] IHC Validation
Message-ID: <F362122314DD5C41BD407A39C4FCF8757644EAC1@AKHV-EX2K12MB3.alaska.ihs.gov>


To All:

I am going to be setting an IHC lab. I would like any suggestions as far as a form for me to use for validating each antibody. I would
like to use a sample form if possible if anyone would like to share.

Thanks!

Karla Arrington, HT(ASCP), HIT(AHIMA)
Lead Histology Technician
kaarrington@anthc.org

From rsrichmond <@t> gmail.com  Sun Sep  7 20:04:06 2014
From: rsrichmond <@t> gmail.com (Bob Richmond)
Date: Sun Sep  7 20:04:13 2014
Subject: [Histonet] Re: Neutrophil staining
Message-ID: <CAOKsRH7cjQpNTo1bmuTcOsXf4qzCWrMZVHv=BPGgk-B+j5bEOA@mail.gmail.com>

Hans B Snyder at Histologistics in Worcester, Massachusetts asks:

>>I am looking to stain neutrophils using special stains and antibodies in
mice tissue. I wanted your opinion about the best methods to do so. I am
thinking of using the May-Gr?newald Giemsa and T-blue for specials and
myeloperoxidase and Ly6G for the antibodies.<<

I haven't done it and don't know if it works on mice, but histochemical
staining for chloroacetate esterase is extremely simple to do, and supposed
to be quite specific.

Bob Richmond
Samurai Pathologist
Maryville, Tennessee
From pruegghm <@t> hotmail.com  Sun Sep  7 20:16:33 2014
From: pruegghm <@t> hotmail.com (Patsy Ruegg)
Date: Sun Sep  7 20:16:37 2014
Subject: [Histonet] Re: Neutrophil staining
In-Reply-To: <CAOKsRH7cjQpNTo1bmuTcOsXf4qzCWrMZVHv=BPGgk-B+j5bEOA@mail.gmail.com>
References: <CAOKsRH7cjQpNTo1bmuTcOsXf4qzCWrMZVHv=BPGgk-B+j5bEOA@mail.gmail.com>
Message-ID: <BAY180-W881B154F38D38FA124DED2D8C10@phx.gbl>

used to cae back in the day and it picked up granules perfectly and was very easy to do

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm@hotmail.com
pruegg@ihctech.net


> Date: Sun, 7 Sep 2014 21:04:06 -0400
> From: rsrichmond@gmail.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Re: Neutrophil staining
> 
> Hans B Snyder at Histologistics in Worcester, Massachusetts asks:
> 
> >>I am looking to stain neutrophils using special stains and antibodies in
> mice tissue. I wanted your opinion about the best methods to do so. I am
> thinking of using the May-Gr?newald Giemsa and T-blue for specials and
> myeloperoxidase and Ly6G for the antibodies.<<
> 
> I haven't done it and don't know if it works on mice, but histochemical
> staining for chloroacetate esterase is extremely simple to do, and supposed
> to be quite specific.
> 
> Bob Richmond
> Samurai Pathologist
> Maryville, Tennessee
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From brett_connolly <@t> merck.com  Mon Sep  8 08:29:35 2014
From: brett_connolly <@t> merck.com (Connolly, Brett M)
Date: Mon Sep  8 08:29:42 2014
Subject: [Histonet] Neutrophil staining
In-Reply-To: <CAAYBjct_WYuDuhi1DY9LtPp7+1uLnQ1_1SfbpK8bdB-3kZRN=g@mail.gmail.com>
References: <CAAYBjct_WYuDuhi1DY9LtPp7+1uLnQ1_1SfbpK8bdB-3kZRN=g@mail.gmail.com>
Message-ID: <C01C35B84DCDCE49BC60867E87F1C8FEA5A17125AA@USCTMXP51015.merck.com>

Hello Hans,

We had very good staining using Ly-6B.2 from Serotec (MCA771)  on C57B6 mice, but needed to switch to Ly-6G for CH3 mice.  The MCA771 datasheet tells you which strains react with that antibody.

Brett

Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly@merck.com
T- 215-652-2501
F- 215-993-6803



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder
Sent: Saturday, September 06, 2014 5:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Neutrophil staining

Hello All,

I am looking to  stain neutrophils using special stains and antibodies
in mice tissue.  I wanted your opinion about the best methods to do
so.  I am thinking of using the may Grunwald giemsa and T-blue for
specials and myeloperoxidase and Ly6G for the antibodies.

What are your favorite special stains and antibodies to stain for neutrophils?

Best regards

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
hans@histologistics.com

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
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New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
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From gu.lang <@t> gmx.at  Mon Sep  8 08:45:23 2014
From: gu.lang <@t> gmx.at (Gudrun Lang)
Date: Mon Sep  8 08:45:36 2014
Subject: AW: [Histonet] Neutrophil staining
In-Reply-To: <C01C35B84DCDCE49BC60867E87F1C8FEA5A17125AA@USCTMXP51015.merck.com>
References: <CAAYBjct_WYuDuhi1DY9LtPp7+1uLnQ1_1SfbpK8bdB-3kZRN=g@mail.gmail.com>
	<C01C35B84DCDCE49BC60867E87F1C8FEA5A17125AA@USCTMXP51015.merck.com>
Message-ID: <000f01cfcb6b$242a12b0$6c7e3810$@gmx.at>

What about PAS staining? Granulozyte granula are PAS positiv.
Gudrun

-----Urspr?ngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Connolly, Brett M
Gesendet: Montag, 08. September 2014 15:30
An: Hans B Snyder; histonet@lists.utsouthwestern.edu
Betreff: RE: [Histonet] Neutrophil staining

Hello Hans,

We had very good staining using Ly-6B.2 from Serotec (MCA771)  on C57B6 mice, but needed to switch to Ly-6G for CH3 mice.  The MCA771 datasheet tells you which strains react with that antibody.

Brett

Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly@merck.com
T- 215-652-2501
F- 215-993-6803



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder
Sent: Saturday, September 06, 2014 5:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Neutrophil staining

Hello All,

I am looking to  stain neutrophils using special stains and antibodies in mice tissue.  I wanted your opinion about the best methods to do so.  I am thinking of using the may Grunwald giemsa and T-blue for specials and myeloperoxidase and Ly6G for the antibodies.

What are your favorite special stains and antibodies to stain for neutrophils?

Best regards

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
hans@histologistics.com

_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.


From stacey_omalley <@t> merck.com  Mon Sep  8 09:20:06 2014
From: stacey_omalley <@t> merck.com (O'Malley, Stacey S.)
Date: Mon Sep  8 09:20:12 2014
Subject: [Histonet] Recommendations for ergonomic cryostat
Message-ID: <8FB2B777A3A80141A40347E5C8F508A3C7646F42A3@USCTMXP51009.merck.com>

I am looking for any recommendations for a more ergonomic cryostat.  I am currently using a Leica CM3050 and CM3050S.  I have been doing a lot of sections lately and looking for better ergonomic options.

If you have any suggestions on improving my current setup, I welcome your thoughts.   I have tried to adjust the height of my chair, but that leads to more leaning over the instrument.

Any suggestions are appreciated.


Stacey O'Malley

Merck & Co. Inc.
Dept. of Imaging
Mail stop - WP44K
office - WP44H-133
(215) 652-6360



Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
From cmiller <@t> physlab.com  Mon Sep  8 10:31:59 2014
From: cmiller <@t> physlab.com (Cheri Miller)
Date: Mon Sep  8 10:32:20 2014
Subject: [Histonet] FW: Thermo-scientific vs Ventana Benchmark XT
In-Reply-To: <E3C81A010935EA41B379AC765103F3BF3E80B1F7C5@olsrv12>
References: <E3C81A010935EA41B379AC765103F3BF3E80B1F7C5@olsrv12>
Message-ID: <E3C81A010935EA41B379AC765103F3BF3E80B1F7C8@olsrv12>


Cheryl A. Miller HT ASCP cm
Histology Supervisor
Hygiene Officer
Physicians Laboratory, P.C.
4840 F St.
Omaha , NE. 68117
402 731 4145 ext. 532
Cell 402 493 0403
Fax 402 731 8653
________________________________
From: Cheri Miller
Sent: Monday, September 08, 2014 10:17 AM
To: histonet-bounces@lists.utsouthwestern.edu
Subject: Thermo-scientific vs Ventana Benchmark XT

Hello , I now have a Ventana Benchmark XT and our company's money people are pushing for us to replace it with the Thermo Auto-stainer 360  to save costs.  I only have the product information promotion folders to go from. Am I to understand that this is an auto-stainer similar to a H&E automated staining? It claims fast turn around and significant cost reduction but it  has many hands on steps in the process as opposed to a "closed system" like Ventana.  Help! I need to communicate the pros and cons of making a change like this I would appreciate any information and opinions you may have.
Thanks, Cheri

Cheryl A. Miller HT ASCP cm
Histology Supervisor
Hygiene Officer
Physicians Laboratory, P.C.
4840 F St.
Omaha , NE. 68117
402 731 4145 ext. 532
Cell 402 493 0403
Fax 402 731 8653

________________________________
PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system.
From cmiller <@t> physlab.com  Mon Sep  8 10:32:13 2014
From: cmiller <@t> physlab.com (Cheri Miller)
Date: Mon Sep  8 10:32:38 2014
Subject: [Histonet] FW: Me again
In-Reply-To: <E3C81A010935EA41B379AC765103F3BF3E80B1F7C6@olsrv12>
References: <E3C81A010935EA41B379AC765103F3BF3E80B1F7C6@olsrv12>
Message-ID: <E3C81A010935EA41B379AC765103F3BF3E80B1F7C9@olsrv12>


________________________________
From: Cheri Miller
Sent: Monday, September 08, 2014 10:31 AM
To: histonet-bounces@lists.utsouthwestern.edu
Subject: Me again

What are peoples thoughts on the Thermo Scientific Excelsior AS processor?

Cheryl A. Miller HT ASCP cm
Histology Supervisor
Hygiene Officer
Physicians Laboratory, P.C.
4840 F St.
Omaha , NE. 68117
402 731 4145 ext. 532
Cell 402 493 0403
Fax 402 731 8653

________________________________
PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system.
From PAMarcum <@t> uams.edu  Mon Sep  8 11:00:06 2014
From: PAMarcum <@t> uams.edu (Marcum, Pamela A)
Date: Mon Sep  8 11:00:12 2014
Subject: [Histonet] RE: Me again
In-Reply-To: <E3C81A010935EA41B379AC765103F3BF3E80B1F7C9@olsrv12>
References: <E3C81A010935EA41B379AC765103F3BF3E80B1F7C6@olsrv12>
	<E3C81A010935EA41B379AC765103F3BF3E80B1F7C9@olsrv12>
Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA320125266C35@Mail2Node2.ad.uams.edu>

We have 4 Excelsiors although the earlier non-touch screen version.  We love them and have had very few problems with them, generally just getting routine maintenance and taking good care for our maintenance is enough.  The reagent exchange is great with no problem there either.  We have multiple programs on each unit ranging from two hour biopsy runs to overnight and gout.  The agitation allowed us to slightly shorten several programs. 

Everyone loves the reagent changing as we are not pulling 12 bottles of solution out to dump at minimum, with our tissue load once a week on four units.   We set the formalin and clean cycles to the number we want to run and it tells us when that number is up and to change them.  The alcohol range is easy to read and know about when to change as is the xylene.  We reduced reagent costs by over 60 to 70% as we added more Excelsiors.  Now I just need to recycle and find a way to reduce cost for the H&E stainers we have at the moment.

Pam Marcum
UAMS

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Monday, September 08, 2014 10:32 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: Me again


________________________________
From: Cheri Miller
Sent: Monday, September 08, 2014 10:31 AM
To: histonet-bounces@lists.utsouthwestern.edu
Subject: Me again

What are peoples thoughts on the Thermo Scientific Excelsior AS processor?

Cheryl A. Miller HT ASCP cm
Histology Supervisor
Hygiene Officer
Physicians Laboratory, P.C.
4840 F St.
Omaha , NE. 68117
402 731 4145 ext. 532
Cell 402 493 0403
Fax 402 731 8653

________________________________
PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

----------------------------------------------------------------------
Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.

From foreightl <@t> gmail.com  Mon Sep  8 11:13:50 2014
From: foreightl <@t> gmail.com (Patrick Laurie)
Date: Mon Sep  8 11:14:00 2014
Subject: [Histonet] FW: Thermo-scientific vs Ventana Benchmark XT
In-Reply-To: <E3C81A010935EA41B379AC765103F3BF3E80B1F7C8@olsrv12>
References: <E3C81A010935EA41B379AC765103F3BF3E80B1F7C5@olsrv12>
	<E3C81A010935EA41B379AC765103F3BF3E80B1F7C8@olsrv12>
Message-ID: <CAKEyg-3yFnBTzh2enDph8BenfEA9tPQTj2pDFGnKwa92m6SxKQ@mail.gmail.com>

Hi Cheri,
The Themo instrument is indeed more work. There is an offline retrieval
system. If the Techs are not trained how to properly do the offline
retrieval, you may have slide failures.  Similar to most stainers, if you
have everything setup well, and good procedures, you can decrease the hands
on time and errors.  This are the same system that Dako uses. The openness
can be a double edged sword though.  You can have lower cost and better
quality, but you have to have someone who is able to work on setting up the
protocols.  If you feel that your lab can do it, you may save quite a bit
of money.

Patrick Laurie(HT)ASCP QIHC

Histology Manager

Celligent Diagnostics, LLC

101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

Work: 704-970-3300  Cell: 704-266-0869

On Mon, Sep 8, 2014 at 11:31 AM, Cheri Miller <cmiller@physlab.com> wrote:

>
> Cheryl A. Miller HT ASCP cm
> Histology Supervisor
> Hygiene Officer
> Physicians Laboratory, P.C.
> 4840 F St.
> Omaha , NE. 68117
> 402 731 4145 ext. 532
> Cell 402 493 0403
> Fax 402 731 8653
> ________________________________
> From: Cheri Miller
> Sent: Monday, September 08, 2014 10:17 AM
> To: histonet-bounces@lists.utsouthwestern.edu
> Subject: Thermo-scientific vs Ventana Benchmark XT
>
> Hello , I now have a Ventana Benchmark XT and our company's money people
> are pushing for us to replace it with the Thermo Auto-stainer 360  to save
> costs.  I only have the product information promotion folders to go from.
> Am I to understand that this is an auto-stainer similar to a H&E automated
> staining? It claims fast turn around and significant cost reduction but it
> has many hands on steps in the process as opposed to a "closed system" like
> Ventana.  Help! I need to communicate the pros and cons of making a change
> like this I would appreciate any information and opinions you may have.
> Thanks, Cheri
>
> Cheryl A. Miller HT ASCP cm
> Histology Supervisor
> Hygiene Officer
> Physicians Laboratory, P.C.
> 4840 F St.
> Omaha , NE. 68117
> 402 731 4145 ext. 532
> Cell 402 493 0403
> Fax 402 731 8653
>
> ________________________________
> PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If
> you are not the addressee intended / indicated or agent responsible for
> delivering it to the addressee, you are hereby notified that you are in
> possession of confidential and privileged information. Any dissemination,
> distribution, or copying of this e-mail is strictly prohibited. If you have
> received this message in error, please notify the sender immediately and
> delete this email from your system.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From JWatson <@t> gnf.org  Mon Sep  8 13:04:13 2014
From: JWatson <@t> gnf.org (James Watson)
Date: Mon Sep  8 13:04:21 2014
Subject: [Histonet] Neutrophil staining
In-Reply-To: <CAAYBjct_WYuDuhi1DY9LtPp7+1uLnQ1_1SfbpK8bdB-3kZRN=g@mail.gmail.com>
References: <CAAYBjct_WYuDuhi1DY9LtPp7+1uLnQ1_1SfbpK8bdB-3kZRN=g@mail.gmail.com>
Message-ID: <A032B25305EC6949AC9AFF23C9620616C9F45914@EX5.lj.gnf.org>



We have been using this Rat antibody in mouse tissue with both fluorescence and Chromogen detection,  The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C.


Neutrophil

Abcam

ab2557

http://www.abcam.com/neutrophil-antibody-nimp-r14-ab2557.html










James Watson HT  ASCP

GNF  Genomics Institute of the Novartis Research Foundation

Scientific Technical Leader II, Histology

Tel    858-332-4647

Fax   858-812-1915

jwatson@gnf.org





-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder
Sent: Saturday, September 06, 2014 2:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Neutrophil staining



Hello All,



I am looking to  stain neutrophils using special stains and antibodies in mice tissue.  I wanted your opinion about the best methods to do so.  I am thinking of using the may Grunwald giemsa and T-blue for specials and myeloperoxidase and Ly6G for the antibodies.



What are your favorite special stains and antibodies to stain for neutrophils?



Best regards



Hans B Snyder

Histologistics

60 Prescott Street

Worcester, MA 01605

508-308-7800

hans@histologistics.com<mailto:hans@histologistics.com>



_______________________________________________

Histonet mailing list

Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu>

http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From cebass <@t> buffalo.edu  Mon Sep  8 15:18:49 2014
From: cebass <@t> buffalo.edu (Bass, Caroline)
Date: Mon Sep  8 15:18:54 2014
Subject: [Histonet] subbing slides for floating brain sections
Message-ID: <0C6B3FBE-9531-4EEA-A92F-C7AE390E9A91@buffalo.edu>

Hi Everyone, 

I typically used charged slides to mount my 50 um floating brain sections. However, these seem pricey and I remember subbing slides in the past. I most do DAB staining, native fluorescence and occasionally immunofluorescence. Can someone tell me a good and easy protocol for cleaning and subbing slides? 

I currently have relatively old gelatin (7+ years?), Type B 225 bloom. Is this sufficient? A lot of protocols call for type A.

Thanks!

Caroline Bass
University at Buffalo
From anna.c.hughes <@t> gsk.com  Mon Sep  8 16:44:11 2014
From: anna.c.hughes <@t> gsk.com (anna.c.hughes)
Date: Mon Sep  8 16:44:17 2014
Subject: [Histonet] Re: Mouse on Mouse Staining
In-Reply-To: <C54F513DA7DA7547B37103A4B74BDAE903CAB9F6@CARRERA.ln.burnham.org>
References: <C54F513DA7DA7547B37103A4B74BDAE903CAB9F6@CARRERA.ln.burnham.org>
Message-ID: <a24c229e-ecbf-47da-9cbf-d3b55d42d043@googlegroups.com>

Hi John! 
 
 GeneTex makes a Rabbit Poly against mouse for HDAC1 : 
http://www.genetex.com/HDAC1-antibody-GTX100513.html   and LSBio makes a 
Rabbit Poly against Nirtrotyrosine : 
http://www.lsbio.com/antibodies/anti-nitrotyrosine-antibody-biotin-ihc-wb-western-elisa-ls-c299307/309222   
I always look for alternative hosts for antibodies rather than do something 
like mouse on mouse first.  I have also had very good luck with with LSBio 
and GeneTex antibodies.  Good Luck!
 
Anna Hughes

*Anna C. Hughes, BBA, BS, HTL (ASCP), QIHC*

Senior Scientist,  Morphologic Pathology 

GlaxoSmithKline

610-239-5771

anna.c.hughes@gsk.com

On Thursday, July 24, 2014 9:11:29 AM UTC-4, John wrote:

>  Good Morning,
>
>  
>
> I have avoided for the last few years doing IHC with mouse on mouse 
> antibodies, but have been requested to do so at this time. I have two 
> antibodies, HDAC1 and Nitrotyrosine from Santa Cruz that I am working up, 
> however the staining pattern in my tissue does not seem to be revealing the 
> accurate staining pattern. I was hoping someone in the research community 
> has worked with these antibodies, even if from other vendors and would be 
> willing to discuss further with me. Thanks!!! 
>
>  
>
> Kind Regards!
>
>  
>
> John J Shelley
>
> Research Specialist, Histology Core Facility
>
> *Sanford-Burnham Medical Research Institute at Lake Nona*
>
>  
>  
From mike <@t> dbiosys.com  Mon Sep  8 17:56:55 2014
From: mike <@t> dbiosys.com (Michael Thompson)
Date: Mon Sep  8 17:57:34 2014
Subject: [Histonet] Re: Mouse on Mouse Staining
In-Reply-To: <a24c229e-ecbf-47da-9cbf-d3b55d42d043@googlegroups.com>
References: <C54F513DA7DA7547B37103A4B74BDAE903CAB9F6@CARRERA.ln.burnham.org>,
	<a24c229e-ecbf-47da-9cbf-d3b55d42d043@googlegroups.com>
Message-ID: <8s9tdclhe29b8nsxvx2qe3q1.1410217032141@email.android.com>

Diagnostic Biosystems has MOM kit.

Look at website below

Email me for a sample



Michael Thompson
Dir of Sales
Diagnostic BioSystems
4128601288
www.dbiosys.com<http://www.dbiosys.com>
"IHC Made Affordable"


Sent from my Verizon Wireless 4G LTE DROID


"anna.c.hughes" <anna.c.hughes@gsk.com> wrote:

Hi John!

 GeneTex makes a Rabbit Poly against mouse for HDAC1 :
http://www.genetex.com/HDAC1-antibody-GTX100513.html   and LSBio makes a
Rabbit Poly against Nirtrotyrosine :
http://www.lsbio.com/antibodies/anti-nitrotyrosine-antibody-biotin-ihc-wb-western-elisa-ls-c299307/309222
I always look for alternative hosts for antibodies rather than do something
like mouse on mouse first.  I have also had very good luck with with LSBio
and GeneTex antibodies.  Good Luck!

Anna Hughes

*Anna C. Hughes, BBA, BS, HTL (ASCP), QIHC*

Senior Scientist,  Morphologic Pathology

GlaxoSmithKline

610-239-5771

anna.c.hughes@gsk.com

On Thursday, July 24, 2014 9:11:29 AM UTC-4, John wrote:

>  Good Morning,
>
>
>
> I have avoided for the last few years doing IHC with mouse on mouse
> antibodies, but have been requested to do so at this time. I have two
> antibodies, HDAC1 and Nitrotyrosine from Santa Cruz that I am working up,
> however the staining pattern in my tissue does not seem to be revealing the
> accurate staining pattern. I was hoping someone in the research community
> has worked with these antibodies, even if from other vendors and would be
> willing to discuss further with me. Thanks!!!
>
>
>
> Kind Regards!
>
>
>
> John J Shelley
>
> Research Specialist, Histology Core Facility
>
> *Sanford-Burnham Medical Research Institute at Lake Nona*
>
>
>
From mike <@t> dbiosys.com  Mon Sep  8 18:08:20 2014
From: mike <@t> dbiosys.com (Michael Thompson)
Date: Mon Sep  8 18:09:11 2014
Subject: [Histonet] Re: Mouse on Mouse Staining
In-Reply-To: <8s9tdclhe29b8nsxvx2qe3q1.1410217032141@email.android.com>
References: <C54F513DA7DA7547B37103A4B74BDAE903CAB9F6@CARRERA.ln.burnham.org>, 
	<a24c229e-ecbf-47da-9cbf-d3b55d42d043@googlegroups.com>,
	<8s9tdclhe29b8nsxvx2qe3q1.1410217032141@email.android.com>
Message-ID: <old4gar4lvjjcvh2up585ek5.1410217705680@email.android.com>

And Rabbit Monos! Website below



Michael Thompson
Dir of Sales
Diagnostic BioSystems
4128601288
www.dbiosys.com<http://www.dbiosys.com>
"IHC Made Affordable"


Sent from my Verizon Wireless 4G LTE DROID


Michael Thompson <mike@dbiosys.com> wrote:

Diagnostic Biosystems has MOM kit.

Look at website below

Email me for a sample



Michael Thompson
Dir of Sales
Diagnostic BioSystems
4128601288
www.dbiosys.com<http://www.dbiosys.com>
"IHC Made Affordable"


Sent from my Verizon Wireless 4G LTE DROID


"anna.c.hughes" <anna.c.hughes@gsk.com> wrote:

Hi John!

 GeneTex makes a Rabbit Poly against mouse for HDAC1 :
http://www.genetex.com/HDAC1-antibody-GTX100513.html   and LSBio makes a
Rabbit Poly against Nirtrotyrosine :
http://www.lsbio.com/antibodies/anti-nitrotyrosine-antibody-biotin-ihc-wb-western-elisa-ls-c299307/309222
I always look for alternative hosts for antibodies rather than do something
like mouse on mouse first.  I have also had very good luck with with LSBio
and GeneTex antibodies.  Good Luck!

Anna Hughes

*Anna C. Hughes, BBA, BS, HTL (ASCP), QIHC*

Senior Scientist,  Morphologic Pathology

GlaxoSmithKline

610-239-5771

anna.c.hughes@gsk.com

On Thursday, July 24, 2014 9:11:29 AM UTC-4, John wrote:

>  Good Morning,
>
>
>
> I have avoided for the last few years doing IHC with mouse on mouse
> antibodies, but have been requested to do so at this time. I have two
> antibodies, HDAC1 and Nitrotyrosine from Santa Cruz that I am working up,
> however the staining pattern in my tissue does not seem to be revealing the
> accurate staining pattern. I was hoping someone in the research community
> has worked with these antibodies, even if from other vendors and would be
> willing to discuss further with me. Thanks!!!
>
>
>
> Kind Regards!
>
>
>
> John J Shelley
>
> Research Specialist, Histology Core Facility
>
> *Sanford-Burnham Medical Research Institute at Lake Nona*
>
>
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From amber.mckenzie <@t> gastrodocs.net  Tue Sep  9 08:33:50 2014
From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie)
Date: Tue Sep  9 08:33:54 2014
Subject: [Histonet] Competency form
In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A0CEAB9@WN35104.or.providence.org>
References: <24C4B3C167E5694887AB594C7602CE3A0CEAB9@WN35104.or.providence.org>
Message-ID: <5A33C952BB67F4468AF1F36D739212BC011252D0EE@JERRY.Gia.com>


I have a new piece of equipment being installed in my lab, does anyone have a generic competency form you'd like to share?  I need something for our staff to sign saying they were trained on it.  Thanks!

From regina.vontell <@t> kcl.ac.uk  Tue Sep  9 09:48:53 2014
From: regina.vontell <@t> kcl.ac.uk (Vontell, Regina)
Date: Tue Sep  9 09:49:03 2014
Subject: [Histonet] RNA scopes
Message-ID: <e8c525c4d4514098a7d790feabf749f3@DB3PR03MB218.eurprd03.prod.outlook.com>

Hi Histoneters,
Does anyone use a RNA scope to quantify insitu hybridization results.
Thanks
Regina
From pruegghm <@t> hotmail.com  Tue Sep  9 16:31:24 2014
From: pruegghm <@t> hotmail.com (Patsy Ruegg)
Date: Tue Sep  9 16:31:30 2014
Subject: [Histonet] HS Class Project
Message-ID: <BAY180-W53D3A753527C571672916AD8CE0@phx.gbl>

Colleagues,

Can
 anyone help me with this request below, I am trying to do a simple IHC 
project in the local HS Biotech class.  I have the reagents donated but I
 need tissue.

I
 am helping Overland HS do some histology in their Biotech class we want
 to do a simple IHC project this year, is there any chance you could get
 us 12 slides prepared from normal pancreas and 6 from abnormal/diabetic
 pancreas, if you have blocks I could make slides and get them back to 
you?

Best regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm@hotmail.com
pruegg@ihctech.net
 		 	   		  
From pruegghm <@t> hotmail.com  Tue Sep  9 17:01:44 2014
From: pruegghm <@t> hotmail.com (Patsy Ruegg)
Date: Tue Sep  9 17:01:49 2014
Subject: [Histonet] HS Class Project
In-Reply-To: <BAY180-W53D3A753527C571672916AD8CE0@phx.gbl>
References: <BAY180-W53D3A753527C571672916AD8CE0@phx.gbl>
Message-ID: <BAY180-W223FE3753B20259F1D04BAD8CE0@phx.gbl>

I forgot to mention these can be human tissues if they are already processed.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm@hotmail.com
pruegg@ihctech.net


> From: pruegghm@hotmail.com
> To: histonet@lists.utsouthwestern.edu
> Date: Tue, 9 Sep 2014 15:31:24 -0600
> Subject: [Histonet] HS Class Project
> 
> Colleagues,
> 
> Can
>  anyone help me with this request below, I am trying to do a simple IHC 
> project in the local HS Biotech class.  I have the reagents donated but I
>  need tissue.
> 
> I
>  am helping Overland HS do some histology in their Biotech class we want
>  to do a simple IHC project this year, is there any chance you could get
>  us 12 slides prepared from normal pancreas and 6 from abnormal/diabetic
>  pancreas, if you have blocks I could make slides and get them back to 
> you?
> 
> Best regards,
> Patsy
> 
> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg IHC Consulting
> 40864 E Arkansas Ave
> Bennett, CO 80102
> H 303-644-4538
> C 720-281-5406
> pruegghm@hotmail.com
> pruegg@ihctech.net
>  		 	   		  _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From kim.tournear <@t> yahoo.com  Tue Sep  9 20:52:07 2014
From: kim.tournear <@t> yahoo.com (Kim Tournear)
Date: Tue Sep  9 20:52:21 2014
Subject: [Histonet] Question?
Message-ID: <6CE7039F-EE90-462B-AB7B-782C9134DD4C@yahoo.com>

All my histonet emails are coming thru my spam folder. Anyone else having this problem? And how can I fix it?
Thanks in advance,

Sent from the iPad of Kim Tournear
From abijag76 <@t> rediffmail.com  Wed Sep 10 09:35:56 2014
From: abijag76 <@t> rediffmail.com (abijag )
Date: Wed Sep 10 09:36:06 2014
Subject: [Histonet] LAMP-2 and adipophilin IHC on mouse(formalin fixed
	paraffin sections)
Message-ID: <20140910143556.18337.qmail@f4mail-235-247.rediffmail.com>

Great folks in histonet,

Can any of you have done or having experience in IHC detection of LAMP-2 and Adipophilin on formalin fixed paraffin sections of mouse. I am working on differentiating phospholipidosis and neutral lipidosis in mouse tissues. please share your experience/protocol.



Thanks very much



Abi Jagannath

Madras veterinary college

India
From tmcampbell <@t> fmh.org  Wed Sep 10 10:52:45 2014
From: tmcampbell <@t> fmh.org (Campbell, Tasha M.)
Date: Wed Sep 10 10:53:44 2014
Subject: [Histonet] Shurwave
Message-ID: <3566D9E34287BE4B95372179009446A021B0E63A@EXCHANGE.fmhnt.fmh.org>

Does anyone out there have a TBS shurwave microwave processor????  My
temperature probe broke and I always keep an extra for when this
happens.  I have replaced them before.  But this time I have replaced it
with a new one and the temperature is still reading over 131C.  Does
anyone know what the problem could be?  It seems to be connected just
fine.  Like I said, I have done it before with no problems.

 

Thank you!!

 

 

 

 

Tasha Campbell, B.S.,HTL(ASCP)

Frederick Gastroenterology Associates

310 W. 9th St.

Frederick, MD 21701

301-695-6800 ext. 144 (w)

304-685-9307 (c)

 

From Lesley.Bechtold <@t> jax.org  Wed Sep 10 11:49:14 2014
From: Lesley.Bechtold <@t> jax.org (Lesley Bechtold)
Date: Wed Sep 10 11:49:21 2014
Subject: [Histonet] Open Position for Histology Services Manager
Message-ID: <08997064E2075247A4DD5A035DB51CDF65006F9E@jaxbhexms02.jax.org>

The Jackson Laboratory (JAX) is an independent nonprofit genetics research institute located in Bar Harbor, Maine.  We are looking for a skilled and motivated individual to fill our Histology Services Manager position.


The Histology Services Manager is responsible for day-to-day oversight of the Histology Service. This includes management of facility, staff and providing training and guidance as needed.  This position requires prioritizing, managing and contributing to the completion of the overall workload in the department.  Additionally the manager is responsible for staff career development and developing and managing the operational budget.


Required Skills:

  *   Excellent verbal and written communication skills, specifically in the development of protocols and interpretation of data
  *   Current computer skills including use of spreadsheet, word processing, email, internet tools in addition to the use of databases and information management systems to record, monitor and analyze workflow
  *   Ability to function in a team environment and work with frequent changes in schedules and priorities

Required Experience:


  *   Bachelor's degree with HT (ASCP) or HTL (ASCP) certification required and eight to ten (8-10) years of histology experience as a certified Histotechnologist
  *   Experience with histological techniques using animal models
  *   Certification in immunohistochemistry (QIHC) is required although equivalent experience (2 or more years) in immunohistochemical techniques may be considered
  *   Three to five (3-5) years of management experience with management experience in a pharmaceutical or research setting desirable



Please submit resume and cover letter to requisition #4554 on www.jax.org/careers.



Lesley S. Bechtold
Senior Manager, Histopathology Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322 (phone)
207-288-6325 (fax)


The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible.
From ttroyer <@t> petersonlab.com  Wed Sep 10 12:22:55 2014
From: ttroyer <@t> petersonlab.com (Travis Troyer)
Date: Wed Sep 10 12:23:01 2014
Subject: [Histonet] (no subject)
Message-ID: <000501cfcd1b$dc5ac120$95104360$@petersonlab.com>

I was wondering what facility labs are sending nerve and muscle biopsies to.


 

Thanks,

Travis Troyer

Peterson Laboratory Services

Manhattan, KS

From wdesalvo.cac <@t> outlook.com  Wed Sep 10 12:31:22 2014
From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO)
Date: Wed Sep 10 12:31:29 2014
Subject: [Histonet] (no subject)
In-Reply-To: <000501cfcd1b$dc5ac120$95104360$@petersonlab.com>
References: <000501cfcd1b$dc5ac120$95104360$@petersonlab.com>
Message-ID: <COL129-W85A32917B974523004F5AC82CF0@phx.gbl>

University of Michigan MLabs

William DeSalvo, BS HTL(ASCP)
 
> From: ttroyer@petersonlab.com
> To: histonet@lists.utsouthwestern.edu
> Date: Wed, 10 Sep 2014 12:22:55 -0500
> Subject: [Histonet] (no subject)
> 
> I was wondering what facility labs are sending nerve and muscle biopsies to.
> 
> 
>  
> 
> Thanks,
> 
> Travis Troyer
> 
> Peterson Laboratory Services
> 
> Manhattan, KS
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From kwalker <@t> valleyhealthlink.com  Wed Sep 10 12:40:56 2014
From: kwalker <@t> valleyhealthlink.com (Walker, Keith)
Date: Wed Sep 10 12:41:01 2014
Subject: [Histonet] Job opening 
Message-ID: <537B732B16C3E142B845DF757628EB1879356ADF@ExchMail4.ds.valleyhealthlink.com>

Job opening for a HTL or HT in Winchester Virginia. In the heart of the beautiful Shenandoah Valley. The position is for a full time Histotechnologist or Histotech with certification. Experience with routine histology and IHC a plus.  Hours are from 08:30 to 5:00 pm Monday through Friday with rotating Saturdays. Great pay and benefits. Send resume to kwalker@valleyhealthlink.com.
From bottlediver <@t> gmail.com  Wed Sep 10 13:12:54 2014
From: bottlediver <@t> gmail.com (Karen)
Date: Wed Sep 10 13:13:03 2014
Subject: [Histonet] (no subject)
In-Reply-To: <COL129-W85A32917B974523004F5AC82CF0@phx.gbl>
References: <000501cfcd1b$dc5ac120$95104360$@petersonlab.com>
	<COL129-W85A32917B974523004F5AC82CF0@phx.gbl>
Message-ID: <960D77DA-0A07-47A5-A764-5D5F0BCEA835@gmail.com>

You could send them to Beaumont Health System. 

Sent from my iPhone

> On Sep 10, 2014, at 1:31 PM, WILLIAM DESALVO <wdesalvo.cac@outlook.com> wrote:
> 
> University of Michigan MLabs
> 
> William DeSalvo, BS HTL(ASCP)
> 
>> From: ttroyer@petersonlab.com
>> To: histonet@lists.utsouthwestern.edu
>> Date: Wed, 10 Sep 2014 12:22:55 -0500
>> Subject: [Histonet] (no subject)
>> 
>> I was wondering what facility labs are sending nerve and muscle biopsies to.
>> 
>> 
>> 
>> 
>> Thanks,
>> 
>> Travis Troyer
>> 
>> Peterson Laboratory Services
>> 
>> Manhattan, KS
>> 
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>                         _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From wtaylor8660 <@t> gmail.com  Thu Sep 11 05:26:10 2014
From: wtaylor8660 <@t> gmail.com (T Williams)
Date: Thu Sep 11 05:26:22 2014
Subject: [Histonet] Re: msg 5 shurwave processor
Message-ID: <CANmnUpZ7SjBxU55FQoCcz+vqYLdW_VqQnLRtBQx+=k4yfhQM8g@mail.gmail.com>

I'll look into it.  In the meantime,  have you set up a service call?

T. Williams,  HTL ( ASCP )
On Sep 10, 2014 1:00 PM, <histonet-request@lists.utsouthwestern.edu> wrote:

> Send Histonet mailing list submissions to
>         histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
>         http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>         histonet-request@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
>         histonet-owner@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>    1. HS Class Project (Patsy Ruegg)
>    2. RE: HS Class Project (Patsy Ruegg)
>    3. Question? (Kim Tournear)
>    4. LAMP-2 and adipophilin IHC on mouse(formalin fixed        paraffin
>       sections) (abijag )
>    5. Shurwave (Campbell, Tasha M.)
>    6. Open Position for Histology Services Manager (Lesley Bechtold)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 9 Sep 2014 15:31:24 -0600
> From: Patsy Ruegg <pruegghm@hotmail.com>
> Subject: [Histonet] HS Class Project
> To: "Histonet@Lists. Edu" <histonet@lists.utsouthwestern.edu>
> Message-ID: <BAY180-W53D3A753527C571672916AD8CE0@phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Colleagues,
>
> Can
>  anyone help me with this request below, I am trying to do a simple IHC
> project in the local HS Biotech class.  I have the reagents donated but I
>  need tissue.
>
> I
>  am helping Overland HS do some histology in their Biotech class we want
>  to do a simple IHC project this year, is there any chance you could get
>  us 12 slides prepared from normal pancreas and 6 from abnormal/diabetic
>  pancreas, if you have blocks I could make slides and get them back to
> you?
>
> Best regards,
> Patsy
>
> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg IHC Consulting
> 40864 E Arkansas Ave
> Bennett, CO 80102
> H 303-644-4538
> C 720-281-5406
> pruegghm@hotmail.com
> pruegg@ihctech.net
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 9 Sep 2014 16:01:44 -0600
> From: Patsy Ruegg <pruegghm@hotmail.com>
> Subject: RE: [Histonet] HS Class Project
> To: "Histonet@Lists. Edu" <histonet@lists.utsouthwestern.edu>
> Message-ID: <BAY180-W223FE3753B20259F1D04BAD8CE0@phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I forgot to mention these can be human tissues if they are already
> processed.
>
> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg IHC Consulting
> 40864 E Arkansas Ave
> Bennett, CO 80102
> H 303-644-4538
> C 720-281-5406
> pruegghm@hotmail.com
> pruegg@ihctech.net
>
>
> > From: pruegghm@hotmail.com
> > To: histonet@lists.utsouthwestern.edu
> > Date: Tue, 9 Sep 2014 15:31:24 -0600
> > Subject: [Histonet] HS Class Project
> >
> > Colleagues,
> >
> > Can
> >  anyone help me with this request below, I am trying to do a simple IHC
> > project in the local HS Biotech class.  I have the reagents donated but I
> >  need tissue.
> >
> > I
> >  am helping Overland HS do some histology in their Biotech class we want
> >  to do a simple IHC project this year, is there any chance you could get
> >  us 12 slides prepared from normal pancreas and 6 from abnormal/diabetic
> >  pancreas, if you have blocks I could make slides and get them back to
> > you?
> >
> > Best regards,
> > Patsy
> >
> > Patsy Ruegg, HT(ASCP)QIHC
> > Ruegg IHC Consulting
> > 40864 E Arkansas Ave
> > Bennett, CO 80102
> > H 303-644-4538
> > C 720-281-5406
> > pruegghm@hotmail.com
> > pruegg@ihctech.net
> >
>  _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 9 Sep 2014 18:52:07 -0700
> From: Kim Tournear <kim.tournear@yahoo.com>
> Subject: [Histonet] Question?
> To: histonet <histonet@lists.utsouthwestern.edu>
> Message-ID: <6CE7039F-EE90-462B-AB7B-782C9134DD4C@yahoo.com>
> Content-Type: text/plain;       charset=us-ascii
>
> All my histonet emails are coming thru my spam folder. Anyone else having
> this problem? And how can I fix it?
> Thanks in advance,
>
> Sent from the iPad of Kim Tournear
>
>
> ------------------------------
>
> Message: 4
> Date: 10 Sep 2014 14:35:56 -0000
> From: "abijag " <abijag76@rediffmail.com>
> Subject: [Histonet] LAMP-2 and adipophilin IHC on mouse(formalin fixed
>         paraffin sections)
> To: <histonet@lists.utsouthwestern.edu>
> Message-ID: <20140910143556.18337.qmail@f4mail-235-247.rediffmail.com>
> Content-Type: text/plain; charset="UTF-8"
>
> Great folks in histonet,
>
> Can any of you have done or having experience in IHC detection of LAMP-2
> and Adipophilin on formalin fixed paraffin sections of mouse. I am working
> on differentiating phospholipidosis and neutral lipidosis in mouse tissues.
> please share your experience/protocol.
>
>
>
> Thanks very much
>
>
>
> Abi Jagannath
>
> Madras veterinary college
>
> India
>
> ------------------------------
>
> Message: 5
> Date: Wed, 10 Sep 2014 11:52:45 -0400
> From: "Campbell, Tasha M." <tmcampbell@fmh.org>
> Subject: [Histonet] Shurwave
> To: <histonet@lists.utsouthwestern.edu>
> Message-ID:
>         <3566D9E34287BE4B95372179009446A021B0E63A@EXCHANGE.fmhnt.fmh.org>
> Content-Type: text/plain;       charset="US-ASCII"
>
> Does anyone out there have a TBS shurwave microwave processor????  My
> temperature probe broke and I always keep an extra for when this
> happens.  I have replaced them before.  But this time I have replaced it
> with a new one and the temperature is still reading over 131C.  Does
> anyone know what the problem could be?  It seems to be connected just
> fine.  Like I said, I have done it before with no problems.
>
>
>
> Thank you!!
>
>
>
>
>
>
>
>
>
> Tasha Campbell, B.S.,HTL(ASCP)
>
> Frederick Gastroenterology Associates
>
> 310 W. 9th St.
>
> Frederick, MD 21701
>
> 301-695-6800 ext. 144 (w)
>
> 304-685-9307 (c)
>
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Wed, 10 Sep 2014 16:49:14 +0000
> From: Lesley Bechtold <Lesley.Bechtold@jax.org>
> Subject: [Histonet] Open Position for Histology Services Manager
> To: "histonet@lists.utsouthwestern.edu"
>         <histonet@lists.utsouthwestern.edu>
> Message-ID:
>         <08997064E2075247A4DD5A035DB51CDF65006F9E@jaxbhexms02.jax.org>
> Content-Type: text/plain; charset="us-ascii"
>
> The Jackson Laboratory (JAX) is an independent nonprofit genetics research
> institute located in Bar Harbor, Maine.  We are looking for a skilled and
> motivated individual to fill our Histology Services Manager position.
>
>
> The Histology Services Manager is responsible for day-to-day oversight of
> the Histology Service. This includes management of facility, staff and
> providing training and guidance as needed.  This position requires
> prioritizing, managing and contributing to the completion of the overall
> workload in the department.  Additionally the manager is responsible for
> staff career development and developing and managing the operational budget.
>
>
> Required Skills:
>
>   *   Excellent verbal and written communication skills, specifically in
> the development of protocols and interpretation of data
>   *   Current computer skills including use of spreadsheet, word
> processing, email, internet tools in addition to the use of databases and
> information management systems to record, monitor and analyze workflow
>   *   Ability to function in a team environment and work with frequent
> changes in schedules and priorities
>
> Required Experience:
>
>
>   *   Bachelor's degree with HT (ASCP) or HTL (ASCP) certification
> required and eight to ten (8-10) years of histology experience as a
> certified Histotechnologist
>   *   Experience with histological techniques using animal models
>   *   Certification in immunohistochemistry (QIHC) is required although
> equivalent experience (2 or more years) in immunohistochemical techniques
> may be considered
>   *   Three to five (3-5) years of management experience with management
> experience in a pharmaceutical or research setting desirable
>
>
>
> Please submit resume and cover letter to requisition #4554 on
> www.jax.org/careers.
>
>
>
> Lesley S. Bechtold
> Senior Manager, Histopathology Sciences
> The Jackson Laboratory
> 600 Main St.
> Bar Harbor, ME 04609
> 207-288-6322 (phone)
> 207-288-6325 (fax)
>
>
> The information in this email, including attachments, may be confidential
> and is intended solely for the addressee(s). If you believe you received
> this email by mistake, please notify the sender by return email as soon as
> possible.
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 130, Issue 10
> *****************************************
>
From ibernard <@t> uab.edu  Thu Sep 11 07:17:23 2014
From: ibernard <@t> uab.edu (Ian R Bernard)
Date: Thu Sep 11 07:17:34 2014
Subject: [Histonet] Paraffin for Infiltration and Embedding & Validation
Message-ID: <D4F4C602B10B9F45B4E9271AF6380E16182C24B6@UABEXMB1.ad.uab.edu>

Does anyone use the same paraffin for both tissue processing  or infiltration and embedding?  We do with quality results; but the company is no longer making that brand. Our brand before was a Fisher brand Paraplast Tissue Embedding Medium that worked well for both infiltration and embedding.  Would like other suggestions.

Per research, the other two alternative proposed and similar brands to what we have are:  distributed by VWR and Fisher- a Leica brand. Any thoughts about them?  There is a difference in price but quality is first then price.

If we find a brand that is made by the same manufacturer as well as chemical composition and temp range, we feel a validation is not necessary.  However, if not, we will do a validation.

Thus, any suggestions on a method or template form concerning validation of a new paraffin for tissue processing and embedding.

The more people respond the more references I have to make an informed decision as to choice and eventually quality.


V/r
Ian R. Bernard, MSHA, HT (ASCP) HTL (Pend-2014)
USAF, MSgt (Retired)
Air Force Red Cross Volunteer Histologist (since 1 June)
Anatomic Patology Technical Supervisor (effective 22 Sep)
Anatomic Pathology, 10th Medical Group
210-687-7540 Cell
ian.bernard@comcast.net<mailto:ian.bernard@comcast.net>


From PAMarcum <@t> uams.edu  Thu Sep 11 08:22:25 2014
From: PAMarcum <@t> uams.edu (Marcum, Pamela A)
Date: Thu Sep 11 08:22:33 2014
Subject: [Histonet] HT Job Opening at UAMS IN Little Rock AR
Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA320125269160@Mail2Node2.ad.uams.edu>

Good Morning,

We have a job opening at the University of Arkansas for Medical Sciences for a registered HT OR HTL.  We have great benefits, retirement and holidays for our employees and a relaxed, busy Histology Laboratory.  We have an active service with routine histology, special stains on the Dako Artisan and limited hand staining. This is an early morning shift.

Little Rock is a beautiful small city with lots outdoor attractions.  It is a beautiful area to live in and has a great low cost of living overall.  For more information you can e-mail me and send a resume.



Best Regards,

Pamela A Marcum
Supervisor AP Hisotlogy
University of Arkansas for Medical Sciences
4301 W Markham Street
Little Rock AR 72205

----------------------------------------------------------------------
Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
From mbireir <@t> yahoo.com  Thu Sep 11 11:15:21 2014
From: mbireir <@t> yahoo.com (Manahil)
Date: Thu Sep 11 11:15:26 2014
Subject: [Histonet] Eosin shades
Message-ID: <FC5AF6CA-19E4-4B18-857F-115FEDD304CC@yahoo.com>


Hi every one
Any one know how to get 3 shades of eosin stain. I am using alcoholic eosin.
Thanks
Manahil Elbireir
HTL/ ASCP


From Kristopher.Kalleberg <@t> unilever.com  Thu Sep 11 11:32:52 2014
From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher)
Date: Thu Sep 11 11:34:49 2014
Subject: [Histonet] papillary dermis
Message-ID: <B83D8FC7C4408B4E9AA95B5E3AB1ECC907CD2D28@CGTSEMB20006.S2.MS.UNILEVER.com>

Hello All,

Does anyone know of a technique, special stain, biomarker, etc.  that will somehow demonstrate the difference between papillary dermis and reticular dermis in skin.  Estimating where the reticular dermis ends and the reticular dermis ends is very subjective.  Any help would be greatly appreciated.  Thanks in advance.

Kris
From Timothy.Morken <@t> ucsfmedctr.org  Thu Sep 11 11:50:32 2014
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy)
Date: Thu Sep 11 11:51:05 2014
Subject: [Histonet] RE: papillary dermis
In-Reply-To: <B83D8FC7C4408B4E9AA95B5E3AB1ECC907CD2D28@CGTSEMB20006.S2.MS.UNILEVER.com>
References: <B83D8FC7C4408B4E9AA95B5E3AB1ECC907CD2D28@CGTSEMB20006.S2.MS.UNILEVER.com>
Message-ID: <761E2B5697F795489C8710BCC72141FF367979A3@ex07.net.ucsf.edu>

Kris, reticular dermis has elastic fibers, papillary dermis does not. So an elastic stain will show fibers in the reticular  area only. 

You can use IHC for collagen types as well. For instance, start with:

http://www.derm101.com/inflammatory/embryologic-histologic-and-anatomic-aspects/collagen/


If you are looking for a bright-line demarcation I think you will be disappointed. There is a gradation between the two so about the best you can do is say the change-over is in a narrow band of the dermis.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher
Sent: Thursday, September 11, 2014 9:33 AM
To: histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu
Subject: [Histonet] papillary dermis

Hello All,

Does anyone know of a technique, special stain, biomarker, etc.  that will somehow demonstrate the difference between papillary dermis and reticular dermis in skin.  Estimating where the reticular dermis ends and the reticular dermis ends is very subjective.  Any help would be greatly appreciated.  Thanks in advance.

Kris
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From chesarato <@t> hotmail.com  Thu Sep 11 12:32:15 2014
From: chesarato <@t> hotmail.com (Cesar Francisco Romero)
Date: Thu Sep 11 12:32:22 2014
Subject: [Histonet] RE: Eosin Shades
Message-ID: <SNT148-W24EA72D3D05BCDC5A4530ABBCC0@phx.gbl>

 
   

I think that the nearest to what you wish is Eosin
- Phloxine Stain.

Here is the web page where you can find the formula.

http://protocolsonline.com/histology/dyes-and-stains/haematoxylin-eosin-he-staining/

 

I use it with very good results.

       

 		 	   		  
From chesarato <@t> hotmail.com  Thu Sep 11 12:36:16 2014
From: chesarato <@t> hotmail.com (Cesar Francisco Romero)
Date: Thu Sep 11 12:36:31 2014
Subject: [Histonet] RE: Papillary Vs Reticular Dermis
Message-ID: <SNT148-W85871C88659381871D2AC7BBCC0@phx.gbl>

 

A good trichrome stain can help very much.

Here is a link to a picture.

http://download.videohelp.com/vitualis/med/his_pic_integument2.htm

 		 	   		  
From llewllew <@t> shaw.ca  Thu Sep 11 12:50:27 2014
From: llewllew <@t> shaw.ca (Bryan Llewellyn)
Date: Thu Sep 11 12:50:38 2014
Subject: [Histonet] RE: Eosin Shades
In-Reply-To: <SNT148-W24EA72D3D05BCDC5A4530ABBCC0@phx.gbl>
References: <SNT148-W24EA72D3D05BCDC5A4530ABBCC0@phx.gbl>
Message-ID: <5411E0E3.6050808@shaw.ca>

Read this page about eosin counterstaining on StainsFile.

Bryan Llewellyn



Cesar Francisco Romero wrote:
>
>
>
> I think that the nearest to what you wish is Eosin
> - Phloxine Stain.
>
> Here is the web page where you can find the formula.
>
> http://protocolsonline.com/histology/dyes-and-stains/haematoxylin-eosin-he-staining/
>
>
>
> I use it with very good results.
>
>
>
>   		 	   		  _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

From tbraud <@t> holyredeemer.com  Thu Sep 11 13:42:57 2014
From: tbraud <@t> holyredeemer.com (Terri  Braud)
Date: Thu Sep 11 13:43:02 2014
Subject: [Histonet] RE: Three shades of Eosin
In-Reply-To: <20140911160832.A0CA31E80F5@trendmess-svr.holyredeemer.local>
References: <20140911160832.A0CA31E80F5@trendmess-svr.holyredeemer.local>
Message-ID: <BFBF7C6B9627B947B74CBEBD45CE368B143B20AD@hrex-svr.holyredeemer.local>

Eosin is differentiated by water content immediately following the Eosin
stain.  This can be accomplished by a water rinse, or rinse in a diluted
alcohol.  I prefer using 70% Alcohol to 95% Alcohol, then 3 washes of
Absolute, all timed to give the desired differentiation for the 3
desired shades.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

Sent: Thursday, September 11, 2014 12:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 130, Issue 11

   8. Eosin shades (Manahil)
Date: Thu, 11 Sep 2014 20:15:21 +0400
From: Manahil <mbireir@yahoo.com>
Subject: [Histonet] Eosin shades
Hi every one
Any one know how to get 3 shades of eosin stain. I am using alcoholic
eosin.
Thanks
Manahil Elbireir
HTL/ ASCP
---------------------------------------------------------------------------------



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it was sent. It may contain information that is privileged and/or confidential,
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federal and state law. If you received this communication in error, please do not
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Please notify the sender of any error by E-Mail.

Thank you for your cooperation.


From mjones <@t> metropath.com  Thu Sep 11 14:00:26 2014
From: mjones <@t> metropath.com (Michael Ann Jones)
Date: Thu Sep 11 14:00:38 2014
Subject: [Histonet] RE: Three shades of Eosin
Message-ID: <D0374CEC.33E2%mjones@metropath.com>

Terri - agreed. We do the same.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones@metropath.com




On 9/11/14, 12:42 PM, "Terri  Braud" <tbraud@holyredeemer.com> wrote:

>Eosin is differentiated by water content immediately following the Eosin
>stain.  This can be accomplished by a water rinse, or rinse in a diluted
>alcohol.  I prefer using 70% Alcohol to 95% Alcohol, then 3 washes of
>Absolute, all timed to give the desired differentiation for the 3
>desired shades.
>
>Terri L. Braud, HT(ASCP)
>Anatomic Pathology Supervisor
>Holy Redeemer Hospital Laboratory
>1648 Huntingdon Pike
>Meadowbrook, PA 19046
>Ph: 215-938-3676
>Fax: 215-938-3874
>
>Sent: Thursday, September 11, 2014 12:09 PM
>To: histonet@lists.utsouthwestern.edu
>Subject: Histonet Digest, Vol 130, Issue 11
>
>   8. Eosin shades (Manahil)
>Date: Thu, 11 Sep 2014 20:15:21 +0400
>From: Manahil <mbireir@yahoo.com>
>Subject: [Histonet] Eosin shades
>Hi every one
>Any one know how to get 3 shades of eosin stain. I am using alcoholic
>eosin.
>Thanks
>Manahil Elbireir
>HTL/ ASCP
>--------------------------------------------------------------------------
>-------
>
>
>
>CONFIDENTIALITY NOTICE:
>
>This E-Mail is intended only for the use of the individual or entity to
>which
>it was sent. It may contain information that is privileged and/or
>confidential,
>and the use or disclosure of such information may also be restricted
>under applicable
>federal and state law. If you received this communication in error,
>please do not
>distribute any part of it or retain any copies, and delete the original
>E-Mail.
>Please notify the sender of any error by E-Mail.
>
>Thank you for your cooperation.
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From llewllew <@t> shaw.ca  Thu Sep 11 14:34:13 2014
From: llewllew <@t> shaw.ca (Bryan Llewellyn)
Date: Thu Sep 11 14:34:29 2014
Subject: [Histonet] RE: Eosin Shades
In-Reply-To: <5411E0E3.6050808@shaw.ca>
References: <SNT148-W24EA72D3D05BCDC5A4530ABBCC0@phx.gbl>
	<5411E0E3.6050808@shaw.ca>
Message-ID: <5411F935.2000501@shaw.ca>

Silly me: http://stainsfile.info/StainsFile/stain/hematoxylin/h-and-e-eo.htm

Bryan


Bryan Llewellyn wrote:
> Read this page about eosin counterstaining on StainsFile.
>
> Bryan Llewellyn
>
>
>
> Cesar Francisco Romero wrote:
>>
>>
>>
>> I think that the nearest to what you wish is Eosin
>> - Phloxine Stain.
>>
>> Here is the web page where you can find the formula.
>>
>> http://protocolsonline.com/histology/dyes-and-stains/haematoxylin-eosin-he-staining/
>>
>>
>>
>>
>> I use it with very good results.
>>
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

From wsimons <@t> athensgastro.com  Thu Sep 11 14:36:26 2014
From: wsimons <@t> athensgastro.com (wsimons@athensgastro.com)
Date: Thu Sep 11 14:36:31 2014
Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gUkU6IFRocmVlIHNoYWRlcyBvZiBFb3Npbg==?=
In-Reply-To: <D0374CEC.33E2%mjones@metropath.com>
References: <D0374CEC.33E2%mjones@metropath.com>
Message-ID: <20140911193626.22615.qmail@server276.com>

Ok, I can't help myself....but all this talk is reminding me of " 50 shades of grey"....
(can't put this on Histonet)

I'll take my "HistoPorn" any day of the week over 50 shades!  
All the pretty pictures under the scope. That is my passion~
I get my "3 shades of eosin" with Richard Allan eosin Y.

Wanda K Simons, HT(ASCP)



>  -------Original Message-------
>  From: Michael Ann Jones <mjones@metropath.com>
>  To: Terri Braud <tbraud@holyredeemer.com>, histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu>
>  Subject: Re: [Histonet] RE: Three shades of Eosin
>  Sent: Sep 11 '14 15:03
>  
>  Terri - agreed. We do the same.
>  Michael Ann Jones, HT (ASCP)
>  Histology Manager
>  Metropath
>  7444 W. Alaska Dr. #250
>  Lakewood, CO 80226
>  303.634.2511
>  Mjones@metropath.com
>  
>  
>  
>  
>  On 9/11/14, 12:42 PM, "Terri??Braud" <tbraud@holyredeemer.com> wrote:
>  
>  >Eosin is differentiated by water content immediately following the Eosin
>  >stain.??This can be accomplished by a water rinse, or rinse in a diluted
>  >alcohol.??I prefer using 70% Alcohol to 95% Alcohol, then 3 washes of
>  >Absolute, all timed to give the desired differentiation for the 3
>  >desired shades.
>  >
>  >Terri L. Braud, HT(ASCP)
>  >Anatomic Pathology Supervisor
>  >Holy Redeemer Hospital Laboratory
>  >1648 Huntingdon Pike
>  >Meadowbrook, PA 19046
>  >Ph: 215-938-3676
>  >Fax: 215-938-3874
>  >
>  >Sent: Thursday, September 11, 2014 12:09 PM
>  >To: histonet@lists.utsouthwestern.edu
>  >Subject: Histonet Digest, Vol 130, Issue 11
>  >
>  >?? 8. Eosin shades (Manahil)
>  >Date: Thu, 11 Sep 2014 20:15:21 +0400
>  >From: Manahil <mbireir@yahoo.com>
>  >Subject: [Histonet] Eosin shades
>  >Hi every one
>  >Any one know how to get 3 shades of eosin stain. I am using alcoholic
>  >eosin.
>  >Thanks
>  >Manahil Elbireir
>  >HTL/ ASCP
>  >--------------------------------------------------------------------------
>  >-------
>  >
>  >
>  >
>  >CONFIDENTIALITY NOTICE:
>  >
>  >This E-Mail is intended only for the use of the individual or entity to
>  >which
>  >it was sent. It may contain information that is privileged and/or
>  >confidential,
>  >and the use or disclosure of such information may also be restricted
>  >under applicable
>  >federal and state law. If you received this communication in error,
>  >please do not
>  >distribute any part of it or retain any copies, and delete the original
>  >E-Mail.
>  >Please notify the sender of any error by E-Mail.
>  >
>  >Thank you for your cooperation.
>  >
>  >
>  >_______________________________________________
>  >Histonet mailing list
>  >Histonet@lists.utsouthwestern.edu
>  >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>  
>  
>  _______________________________________________
>  Histonet mailing list
>  Histonet@lists.utsouthwestern.edu
>  http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>  

From Lacie.Algeo <@t> providence.org  Thu Sep 11 17:23:59 2014
From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A)
Date: Thu Sep 11 17:24:16 2014
Subject: [Histonet] MOHS
Message-ID: <24C4B3C167E5694887AB594C7602CE3A0D52DC@WN35104.or.providence.org>

We are looking at bringing MOHS in-house for a new physician.  We already do a great deal of frozen sections, so we are set up for cryotomy and staining, however, I am in need of a good procedure for embedding, cryotomy etc.
Thank you,
Lacie

Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
lacie.algeo@providence.org<mailto:lacie.algeo@providence.org>


This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law.  If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message.  If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message.


________________________________

This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message.
From chesarato <@t> hotmail.com  Fri Sep 12 01:17:54 2014
From: chesarato <@t> hotmail.com (Cesar Francisco Romero)
Date: Fri Sep 12 01:18:06 2014
Subject: [Histonet] Re: Papillary Vs Reticular Dermis.
Message-ID: <SNT148-W36C520132A0B9F00E2CDB1BBCD0@phx.gbl>

 

Another Hallmark is the Superficial Vascular Plexus of
the Skin. 

You can do Muscle Actin ( HHF ? 35 ) or CD 34 to
remark the vessels. 

Link to a Picture 

http://biologiedelapeau.fr/spip.php?page=forum&id_article=21&lang=en 

This Superficial Vascular Plexus allows separating
Invasive Melanoma Clark?s Level III from Level IV.

 		 	   		  
From GKeyser <@t> uwhealth.org  Fri Sep 12 08:28:53 2014
From: GKeyser <@t> uwhealth.org (Keyser Gerald  T)
Date: Fri Sep 12 08:28:59 2014
Subject: [Histonet] RE: MOHS
In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A0D52DC@WN35104.or.providence.org>
References: <24C4B3C167E5694887AB594C7602CE3A0D52DC@WN35104.or.providence.org>
Message-ID: <5226C88C65EBFF4BAD552D68DC6E8FFE03803C@UWHC-MBX12.uwhis.hosp.wisc.edu>

Embedding:

I use a large, solid aluminum block that I keep cool in my cryostat. It's approx. 9 in (length) x 4 in (width) x 3 in (depth). I just stick the epidermal edge to the block and flatten it out so all margins are in contact with the freezing surface. Drop some freezing medium on it and attach it to a specimen holder. It's good to go. 

A large, thick, removable metal block as a freezing surface should be standard equipment for all cryostats. 

Sectioning: 

Having a liquid nitrogen gun to selectively freeze fatty constituents in the specimen is a good idea. 

After I collect the specimens on a slide I check it with a microscope with the light source turned on very low. That allows the tech to see if the epidermis is completely represented in the specimen. Other techs I work with do it a bit differently. But, the results I get from a quick examination under a microscope is excellent.  

 Gerald Keyser

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A
Sent: Thursday, September 11, 2014 5:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] MOHS

We are looking at bringing MOHS in-house for a new physician.  We already do a great deal of frozen sections, so we are set up for cryotomy and staining, however, I am in need of a good procedure for embedding, cryotomy etc.
Thank you,
Lacie

Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
lacie.algeo@providence.org<mailto:lacie.algeo@providence.org>


This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law.  If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message.  If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message.


________________________________

This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From veronique.barres <@t> gmail.com  Fri Sep 12 09:32:41 2014
From: veronique.barres <@t> gmail.com (=?UTF-8?B?VsOpcm9uaXF1ZSBCYXJyw6hz?=)
Date: Fri Sep 12 09:33:25 2014
Subject: [Histonet] Blades for cutting resin on a microtome
Message-ID: <CABEzr_H9An1sd_+MijBtLHZF3Auo=_Otq2UsJR2rb17b5M8H5w@mail.gmail.com>

Happy Friday Histonetters!

I am working on a histology platform in a research center and someone came
to me last week and asked to cut blocs of resin (JB-4 resin) on the
microtome. I never cut anything else than paraffin, so I was wondering if
some of you had advices for me?

They never did it neither and took their protocol in a paper where it was
said that we should use disposable glass knife instead of standard metal
blades. Are any of you ever used those knife? Where do you buy them?
We have an old Leica RM2125.

Thanks for your advices!

V?ronique
From ratliffjack <@t> hotmail.com  Fri Sep 12 09:46:36 2014
From: ratliffjack <@t> hotmail.com (Jack Ratliff)
Date: Fri Sep 12 09:46:43 2014
Subject: [Histonet] Blades for cutting resin on a microtome
In-Reply-To: <CABEzr_H9An1sd_+MijBtLHZF3Auo=_Otq2UsJR2rb17b5M8H5w@mail.gmail.com>
References: <CABEzr_H9An1sd_+MijBtLHZF3Auo=_Otq2UsJR2rb17b5M8H5w@mail.gmail.com>
Message-ID: <BLU437-SMTP99B1043F18252479534816AECD0@phx.gbl>

Veronique,

May I ask what type of specimen is embedded into the JB-4 resin? Nevertheless, you should be able to cut these blocks using a tungsten-carbide knife. While there are a few vendors out there that sell these knives, in my laboratory I personally use knives re-sharpened by Delaware Diamond Knives (DDK). Please feel free to message me privately if you need further assistance as I have been working with resin embedded specimens for over 17 years.

I will also encourage you to reach out to Sarah Mack as she is the new Hard Tissue Committee Chairperson for the National Society for Histotechnology. You can find her contact information and additional information about the committee by visiting www.nsh.org!

Best Regards,

Jack Ratliff




> On Sep 12, 2014, at 9:32 AM, V?ronique Barr?s <veronique.barres@gmail.com> wrote:
> 
> Happy Friday Histonetters!
> 
> I am working on a histology platform in a research center and someone came
> to me last week and asked to cut blocs of resin (JB-4 resin) on the
> microtome. I never cut anything else than paraffin, so I was wondering if
> some of you had advices for me?
> 
> They never did it neither and took their protocol in a paper where it was
> said that we should use disposable glass knife instead of standard metal
> blades. Are any of you ever used those knife? Where do you buy them?
> We have an old Leica RM2125.
> 
> Thanks for your advices!
> 
> V?ronique
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

From GKeyser <@t> uwhealth.org  Fri Sep 12 09:46:57 2014
From: GKeyser <@t> uwhealth.org (Keyser Gerald  T)
Date: Fri Sep 12 09:47:03 2014
Subject: [Histonet] Blades for cutting resin on a microtome
In-Reply-To: <CABEzr_H9An1sd_+MijBtLHZF3Auo=_Otq2UsJR2rb17b5M8H5w@mail.gmail.com>
References: <CABEzr_H9An1sd_+MijBtLHZF3Auo=_Otq2UsJR2rb17b5M8H5w@mail.gmail.com>
Message-ID: <5226C88C65EBFF4BAD552D68DC6E8FFE038058@UWHC-MBX12.uwhis.hosp.wisc.edu>

I've only cut resin with a glass or diamond knife in an ultramicrotome. If you are attempting to do it in a regular microtome, you would need a special blade holder. I don't know if any microtome manufactures make glass knife holders. 

You make the glass blades yourself using special glass. Here is a link to the glass strips: http://www.sigmaaldrich.com/catalog/product/sigma/g2528?lang=en&region=US

Here is a cheap jig and diamond glass cutters it make the knifes:
https://www.emsdiasum.com/microscopy/products/preparation/glassknife.aspx

I've never made glass knives by hand using a hand held diamond cutter and jigs. I imagine that it would take practice.  

I've only used a specialized maker:
https://www.emsdiasum.com/microscopy/products/histology/tissue_stainer.aspx

You paint a bit of nail polish underneath the glass edge and put a bit of distilled water on the edge. You then section the block floating the sections on the water. Use an eyelash manipulator to pick up the 5um thick sections and place on a bubble of water on the slide. Evaporate the water droplet on the slide. If you've done it right, the sections won't look like origami. If it does, then practice until it doesn't. 

Gerry 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of V?ronique Barr?s
Sent: Friday, September 12, 2014 9:33 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Blades for cutting resin on a microtome

Happy Friday Histonetters!

I am working on a histology platform in a research center and someone came to me last week and asked to cut blocs of resin (JB-4 resin) on the microtome. I never cut anything else than paraffin, so I was wondering if some of you had advices for me?

They never did it neither and took their protocol in a paper where it was said that we should use disposable glass knife instead of standard metal blades. Are any of you ever used those knife? Where do you buy them?
We have an old Leica RM2125.

Thanks for your advices!

V?ronique
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From abright <@t> brightinstruments.com  Fri Sep 12 11:32:47 2014
From: abright <@t> brightinstruments.com (Alan Bright)
Date: Fri Sep 12 11:33:04 2014
Subject: [Histonet] Blades for cutting resin on a microtome
In-Reply-To: <5226C88C65EBFF4BAD552D68DC6E8FFE038058@UWHC-MBX12.uwhis.hosp.wisc.edu>
References: <CABEzr_H9An1sd_+MijBtLHZF3Auo=_Otq2UsJR2rb17b5M8H5w@mail.gmail.com>
	<5226C88C65EBFF4BAD552D68DC6E8FFE038058@UWHC-MBX12.uwhis.hosp.wisc.edu>
Message-ID: <3535AD93-5EB3-42CF-ACD8-8EA4234A5BA0@brightinstruments.com>

Yes brightinstruments, com make glass knife holder and tungsten carbide tipped knives for microtomes,
KR,,,,,Alan Bright

Sent from my iPhone

> On 12 Sep 2014, at 15:49, "Keyser Gerald  T" <GKeyser@uwhealth.org> wrote:
> 
> I've only cut resin with a glass or diamond knife in an ultramicrotome. If you are attempting to do it in a regular microtome, you would need a special blade holder. I don't know if any microtome manufactures make glass knife holders. 
> 
> You make the glass blades yourself using special glass. Here is a link to the glass strips: http://www.sigmaaldrich.com/catalog/product/sigma/g2528?lang=en&region=US
> 
> Here is a cheap jig and diamond glass cutters it make the knifes:
> https://www.emsdiasum.com/microscopy/products/preparation/glassknife.aspx
> 
> I've never made glass knives by hand using a hand held diamond cutter and jigs. I imagine that it would take practice.  
> 
> I've only used a specialized maker:
> https://www.emsdiasum.com/microscopy/products/histology/tissue_stainer.aspx
> 
> You paint a bit of nail polish underneath the glass edge and put a bit of distilled water on the edge. You then section the block floating the sections on the water. Use an eyelash manipulator to pick up the 5um thick sections and place on a bubble of water on the slide. Evaporate the water droplet on the slide. If you've done it right, the sections won't look like origami. If it does, then practice until it doesn't. 
> 
> Gerry 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of V?ronique Barr?s
> Sent: Friday, September 12, 2014 9:33 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Blades for cutting resin on a microtome
> 
> Happy Friday Histonetters!
> 
> I am working on a histology platform in a research center and someone came to me last week and asked to cut blocs of resin (JB-4 resin) on the microtome. I never cut anything else than paraffin, so I was wondering if some of you had advices for me?
> 
> They never did it neither and took their protocol in a paper where it was said that we should use disposable glass knife instead of standard metal blades. Are any of you ever used those knife? Where do you buy them?
> We have an old Leica RM2125.
> 
> Thanks for your advices!
> 
> V?ronique
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> -- 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From bburnett <@t> CapeCodHealth.org  Fri Sep 12 12:24:56 2014
From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy)
Date: Fri Sep 12 12:25:09 2014
Subject: [Histonet] Re: Papillary Vs Reticular Dermis.
In-Reply-To: <SNT148-W36C520132A0B9F00E2CDB1BBCD0@phx.gbl>
References: <SNT148-W36C520132A0B9F00E2CDB1BBCD0@phx.gbl>
Message-ID: <C7CEB8BE7DF69446817C237018344D0004C5DB@FHEXMB1.cchdomain1.capecodhealth.org>

You can use Smooth Muscle Actin, Calponin, Myosin and CD34 to stain the vessels.

________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cesar Francisco Romero [chesarato@hotmail.com]
Sent: Friday, September 12, 2014 2:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Papillary Vs Reticular Dermis.

Another Hallmark is the Superficial Vascular Plexus of
the Skin.

You can do Muscle Actin ( HHF ? 35 ) or CD 34 to
remark the vessels.

Link to a Picture

http://biologiedelapeau.fr/spip.php?page=forum&id_article=21&lang=en

This Superficial Vascular Plexus allows separating
Invasive Melanoma Clark?s Level III from Level IV.

                                          _______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error  contact the Help Desk for Cape Cod Healthcare.

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From cls71877 <@t> gmail.com  Fri Sep 12 12:37:16 2014
From: cls71877 <@t> gmail.com (Cristi Stephenson)
Date: Fri Sep 12 12:37:22 2014
Subject: [Histonet] Tape coverslipper
Message-ID: <CAMjZ=fadb1FkDbei5pxVjhwk-f556+mqGzHDUaR=mutmNrBjTg@mail.gmail.com>

Happy Friday Histopeeps!

I am looking for opinions on tape coverslippers.  I currently have a Leica
CV5030 and while it is only 5 years young, it can be a bit petulant.  I
would like to find something refurbished, but haven't had any luck.  We are
a low volume lab averaging 100 slides/day.  Any input or leads would be
greatly appreciated!

Thanks and have a great weekend!
Cristi
From blayjorge <@t> gmail.com  Fri Sep 12 12:51:41 2014
From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay)
Date: Fri Sep 12 12:51:51 2014
Subject: [Histonet] nigrosin and eosin negative staining
Message-ID: <CAGBdDuCLZPKs5Px_2n_fqYgLjZ8=FQ56_4fHEnEtUqie_=Gnyg@mail.gmail.com>

Dear Histonet Listers:

Other than nigrosin stain or eosin stain, do you know what other stains are
good for negative staining of bacteria. While I am aware of the principles
of negative staining, I would like to know what have been used successfully
for this simple technique.

If you know of other specific stains, could you email me directly (
blayjorge@gmail.com)? Thank you.

Sincerely,

Jorge

Jorge A. Santiago-Blay, PhD
blaypublishers.com
http://blayjorge.wordpress.com/
http://paleobiology.si.edu/staff/individuals/santiagoblay.html

P.S. Apologies if you have received this email more than once.
From Lynn.Burton <@t> Illinois.gov  Fri Sep 12 14:27:04 2014
From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn)
Date: Fri Sep 12 14:27:15 2014
Subject: [Histonet] Tape coverslipper
In-Reply-To: <CAMjZ=fadb1FkDbei5pxVjhwk-f556+mqGzHDUaR=mutmNrBjTg@mail.gmail.com>
References: <CAMjZ=fadb1FkDbei5pxVjhwk-f556+mqGzHDUaR=mutmNrBjTg@mail.gmail.com>
Message-ID: <ED8BB12FF8B98347BE7A3BAE9087B546565E3414@il084exmbx43.illinois.gov>

We have had a Sakura tape coverslipper for 17 years with hardly any problems. The slide storage quality is excellent.


Lynn M Burton
Histology
Animal Disease Lab
Galesburg, Il
309-344-2451



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristi Stephenson
Sent: Friday, September 12, 2014 12:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tape coverslipper

Happy Friday Histopeeps!

I am looking for opinions on tape coverslippers.  I currently have a Leica
CV5030 and while it is only 5 years young, it can be a bit petulant.  I would like to find something refurbished, but haven't had any luck.  We are a low volume lab averaging 100 slides/day.  Any input or leads would be greatly appreciated!

Thanks and have a great weekend!
Cristi
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From raestask <@t> grics.net  Fri Sep 12 15:33:17 2014
From: raestask <@t> grics.net (Rae Staskiewicz)
Date: Fri Sep 12 15:33:30 2014
Subject: [Histonet] C-Myc
Message-ID: <003e01cfcec8$c95d26e0$5c1774a0$@grics.net>

Would anyone have a protocol for C-Myc using the Ventana Benchmark Ultra or
XT? Thanks in advance for any information you can provide.

 

Rae Ann Staskiewicz

UnityPoint Health Methodist

Peoria, IL

309-672-5994

From LSebree <@t> uwhealth.org  Fri Sep 12 15:44:32 2014
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Fri Sep 12 15:44:38 2014
Subject: [Histonet] C-Myc
In-Reply-To: <003e01cfcec8$c95d26e0$5c1774a0$@grics.net>
References: <003e01cfcec8$c95d26e0$5c1774a0$@grics.net>
Message-ID: <77DD817201982748BC67D7960F2F76AF0DB6DB@UWHC-MBX12.uwhis.hosp.wisc.edu>

We do. I'll email Tuesday when I'm back at work.

Linda A. Sebree
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Rae Staskiewicz [raestask@grics.net]
Sent: Friday, September 12, 2014 3:33 PM
To: Histonet
Subject: [Histonet] C-Myc

Would anyone have a protocol for C-Myc using the Ventana Benchmark Ultra or
XT? Thanks in advance for any information you can provide.



Rae Ann Staskiewicz

UnityPoint Health Methodist

Peoria, IL

309-672-5994

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From GoetzF <@t> si.edu  Fri Sep 12 16:06:44 2014
From: GoetzF <@t> si.edu (Goetz, Freya E.)
Date: Fri Sep 12 16:06:48 2014
Subject: [Histonet] Tissue embedding station
Message-ID: <8D7028B1-1458-47AB-8918-B11AC4A20E7A@si.edu>

Hello All,

We have a Leica histoembedder that is on the fritz. It works well and is quite convenient as long as it decides to turn on. Leica is insanely expensive for any service call and, depending on the price of the embedder, it could be cheaper to buy a new one.

I am writing to find out if anyone has opinions on tissue embedding equipment. Our lab is small and we don't have more than one person working at a time but hopefully in the future we will have more users. Money is definitely a constraint but we don't want to throw away money at a machine that won't last a long time.

We are looking into modular options and going very simple (e.g. Paraffin dispenser and metal plate that we would keep in the freezer). The latter would be very cheap but I'm not sure if it would be ideal. Our current machine has the problem that if the cooling plate breaks down, the whole machine won't work and like I said, Leica has ridiculous service prices.

I can see that the great price differences in embedding stations depend on the number of bells and whistles but I am not convinced that they are worth the additional price. Does anyone have a brand they like or dislike? Does anyone have an opinion on getting a modular embedder where the cooling plate is separate from the heating module but they fit together instead of a one-piece embedder? Is it worth it to get an embedder that is $13K over a $4k-$8K machine? I am also wondering if anyone has a lab without an embedding machine and if so, what equipment you have.

I like EMS as a company and they have two embedders but I have no idea about the quality of their equipment. I have only ordered reagents and consumables from EMS. So far they have never disappointed me. Ted Pella also has a reasonably priced modular embedding station that looks alright.

Any cents from the wise and experienced are greatly appreciated.

Thank you in advance!

Freya



Museum Technician
Department of Invertebrate Zoology
Smithsonian National Museum of Natural History
Washington, DC



From andre.charbonneau2 <@t> mail.mcgill.ca  Fri Sep 12 16:11:25 2014
From: andre.charbonneau2 <@t> mail.mcgill.ca (=?iso-8859-1?Q?Andr=E9_Charbonneau?=)
Date: Fri Sep 12 16:11:32 2014
Subject: [Histonet] Metamorphosed Axolotl Heads
Message-ID: <EF5799FA-FDDA-44AD-92CD-1F5280B25D01@mail.mcgill.ca>

Does anybody have any paraffin embedded Metamophosed Axolotl Heads?

Andr? Charbonneau
M.Sc. Candidate
Faculty of Dentistry 
McGill University




From pruegghm <@t> hotmail.com  Fri Sep 12 17:23:01 2014
From: pruegghm <@t> hotmail.com (Patsy Ruegg)
Date: Fri Sep 12 17:23:07 2014
Subject: [Histonet] Blades for cutting resin on a microtome
In-Reply-To: <3535AD93-5EB3-42CF-ACD8-8EA4234A5BA0@brightinstruments.com>
References: <CABEzr_H9An1sd_+MijBtLHZF3Auo=_Otq2UsJR2rb17b5M8H5w@mail.gmail.com>,
	<5226C88C65EBFF4BAD552D68DC6E8FFE038058@UWHC-MBX12.uwhis.hosp.wisc.edu>,
	<3535AD93-5EB3-42CF-ACD8-8EA4234A5BA0@brightinstruments.com>
Message-ID: <BAY180-W3804702EE13A0FEA108496D8CD0@phx.gbl>

I used to have a triangle glass knife holder insert for my Leica microtome or I would use the tungsten carbide knives.  It depends on what you are cutting.  if it is calcified bone the glass knives scratch too much and they are only 1/2 inch wide so you have to cut smaller soft tissues with them.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm@hotmail.com
pruegg@ihctech.net


From: abright@brightinstruments.com
Date: Fri, 12 Sep 2014 17:32:47 +0100
To: GKeyser@uwhealth.org
Subject: Re: [Histonet] Blades for cutting resin on a microtome
CC: histonet@lists.utsouthwestern.edu; veronique.barres@gmail.com; export@brightinstruments.com

Yes brightinstruments, com make glass knife holder and tungsten carbide tipped knives for microtomes,
KR,,,,,Alan Bright
 
Sent from my iPhone
 
> On 12 Sep 2014, at 15:49, "Keyser Gerald  T" <GKeyser@uwhealth.org> wrote:
> 
> I've only cut resin with a glass or diamond knife in an ultramicrotome. If you are attempting to do it in a regular microtome, you would need a special blade holder. I don't know if any microtome manufactures make glass knife holders. 
> 
> You make the glass blades yourself using special glass. Here is a link to the glass strips: http://www.sigmaaldrich.com/catalog/product/sigma/g2528?lang=en&region=US
> 
> Here is a cheap jig and diamond glass cutters it make the knifes:
> https://www.emsdiasum.com/microscopy/products/preparation/glassknife.aspx
> 
> I've never made glass knives by hand using a hand held diamond cutter and jigs. I imagine that it would take practice.  
> 
> I've only used a specialized maker:
> https://www.emsdiasum.com/microscopy/products/histology/tissue_stainer.aspx
> 
> You paint a bit of nail polish underneath the glass edge and put a bit of distilled water on the edge. You then section the block floating the sections on the water. Use an eyelash manipulator to pick up the 5um thick sections and place on a bubble of water on the slide. Evaporate the water droplet on the slide. If you've done it right, the sections won't look like origami. If it does, then practice until it doesn't. 
> 
> Gerry 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of V?ronique Barr?s
> Sent: Friday, September 12, 2014 9:33 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Blades for cutting resin on a microtome
> 
> Happy Friday Histonetters!
> 
> I am working on a histology platform in a research center and someone came to me last week and asked to cut blocs of resin (JB-4 resin) on the microtome. I never cut anything else than paraffin, so I was wondering if some of you had advices for me?
> 
> They never did it neither and took their protocol in a paper where it was said that we should use disposable glass knife instead of standard metal blades. Are any of you ever used those knife? Where do you buy them?
> We have an old Leica RM2125.
> 
> Thanks for your advices!
> 
> V?ronique
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> -- 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 		 	   		  
From christina.kreutzer01 <@t> gmail.com  Mon Sep 15 10:11:30 2014
From: christina.kreutzer01 <@t> gmail.com (Christina Kreutzer)
Date: Mon Sep 15 10:11:33 2014
Subject: [Histonet] mounting media for immunofluorescence
Message-ID: <CANS3E9vOkzX8dtVuL=5KO18sqUrSuz-G92EV-7bHmcb1C0CSHQ@mail.gmail.com>

Hello folks,
I am wondering which mounting medium f?r IF you are using in your daily
routine.
We are using Prolong antifade gold, but it is really really really
expensive and I was wondering if there is a good alternative. I just
ordered a sample of the Dako Fluorescence Mounting Medium so we can try
that , but we are a little bit concerned about how long lasting the
fluorescence signal would be.

Well, what are your experiences so far.

All the best
Christina
From jpiche <@t> wtbyhosp.org  Mon Sep 15 10:16:57 2014
From: jpiche <@t> wtbyhosp.org (Piche, Jessica)
Date: Mon Sep 15 10:17:06 2014
Subject: [Histonet] MERIFLUOR DFA Pneumocystis
Message-ID: <631955447A364B45B9458D2905635110CBBA7AAA@WIN08-MBX-02.wtbyhosp.org>

Good Morning,

I was wondering if anyone uses the Merifluor DFA kit for Pneumocystis and if so where can you buy controls for it? We have had no positive cases to make our own cytospin controls.

Thank you,

Jessica Piche, HT(ASCP)
Waterbury Hospital



CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital
From csegovia <@t> nsalabs.com  Mon Sep 15 10:35:39 2014
From: csegovia <@t> nsalabs.com (Segovia, Claudia)
Date: Mon Sep 15 10:36:19 2014
Subject: [Histonet] mounting media for immunofluorescence
In-Reply-To: <CANS3E9vOkzX8dtVuL=5KO18sqUrSuz-G92EV-7bHmcb1C0CSHQ@mail.gmail.com>
References: <CANS3E9vOkzX8dtVuL=5KO18sqUrSuz-G92EV-7bHmcb1C0CSHQ@mail.gmail.com>
Message-ID: <D679BFF5-04BB-4754-A74B-BB867EC84F03@nsalabs.com>

Hi Christina,

We use DPX. It has worked very well for us. I am not sure about the cost though, but anything bought in kits can get really pricy. DPX comes in 500 ml.

Claudia N. Segovia
Senior Neurohistologist
Antibody Specialist
NeuroScience Associates
10915 Lake Ridge Drive
Knoxville, TN  37934
865-675-2245
csegovia@nsalabs.com

STATEMENT OF CONFIDENTIALITY:
The information contained in this electronic message and any attachments are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not the intended recipient, or the person responsible for delivering email to the intended recipient, be advised you have received this message in error and any use, dissemination, forwarding, printing or copying is strictly prohibited. Please notify NeuroScience Associates immediately at 865-675-2245 or at csegovia@nsalabs.com, and destroy all copies of this message and any attachments. You will be reimbursed for reasonable costs incurred in notifying us.

> On Sep 15, 2014, at 11:21 AM, "Christina Kreutzer" <christina.kreutzer01@gmail.com> wrote:
> 
> Hello folks,
> I am wondering which mounting medium f?r IF you are using in your daily
> routine.
> We are using Prolong antifade gold, but it is really really really
> expensive and I was wondering if there is a good alternative. I just
> ordered a sample of the Dako Fluorescence Mounting Medium so we can try
> that , but we are a little bit concerned about how long lasting the
> fluorescence signal would be.
> 
> Well, what are your experiences so far.
> 
> All the best
> Christina
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From klaus.dern44 <@t> gmail.com  Mon Sep 15 11:31:48 2014
From: klaus.dern44 <@t> gmail.com (Klaus Dern)
Date: Mon Sep 15 11:31:58 2014
Subject: [Histonet] THICK AND THIN SECTIONS ?
Message-ID: <CAJhryitbvd9A4DoPrETRCod9SqcjG+hn0JKWepN1q=tbzAHKNA@mail.gmail.com>

If you are using one of the following Microtomes and the advance mechanism
is worn out.
( too much spindle play )

REICHERT/JUNG 2030
LEICA            RM 2125
LEICA                  2030 Biocut
LEICA/JUNG        2035
LEICA  - CM         1850 Cryostat
SAKURA  SRM      200


You could be faced with purchasing a new Microtome because these Models are
no longer supported by the Manufacturer. ( No parts availability )

Rather than replacing these excellent Instruments, I have a PERMANENT
solution to fix this problem.

For Information please contact:

Klaus Dern
Phone: 706 635-8840
E-Mail:  klaus.dern44@gmail.com
From klaus.dern44 <@t> gmail.com  Mon Sep 15 11:54:00 2014
From: klaus.dern44 <@t> gmail.com (Klaus Dern)
Date: Mon Sep 15 11:54:04 2014
Subject: [Histonet] THICK AND THIN SECTIONS ?
Message-ID: <CAJhryitmzVgSh6MgMPMbNG+e_GCgbNJDc64Kb1mf6L0R9=mVeg@mail.gmail.com>

If you are using one of the following Microtomes and the advance mechanism
is worn out.
( too much spindle play)

REICHERT/JUNG  2030
LEICA           RM  2125
LEICA                  2030 Biocut
LEICA/JUNG        2035
LEICA -CM           1800 Cryostat
SAKURA - SRM     200

You could be faced with purchasing a new Microtome, because these Models
are no longer supported by the Manufacturer. ( No parts availability )

Rather than replacing these excellent Instruments, I have a PERMANENT
solution to fix this problem.

For Information, please contact:

Klaus Dern
Phone: 706 635-8840
E-Mail:  klaus.dern44@gmail.com
From tkngflght <@t> yahoo.com  Mon Sep 15 12:15:19 2014
From: tkngflght <@t> yahoo.com (Cheryl)
Date: Mon Sep 15 12:15:29 2014
Subject: [Histonet] Job opening: grosser 
Message-ID: <1410801319.65472.YahooMailNeo@web161205.mail.bf1.yahoo.com>

Good morning!

Open position-- a grosser for a new derm lab.  It's in the Northeast right on the Atlantic Ocean  Beautiful location, good hours.  You do not have to be a registered histotech: CLIA '88 gross-qualified is required. (Not sure what that is--call me!)

All inquiries welcome.

Other positions available-- this one is the most pressing.

Thank you!


 
Cheryl Kerry, HT(ASCP) 
Full Staff Inc. 
Staffing the AP Lab by helping one GREAT Tech at a time.  
281.852.9457 Office
800.756.3309 Phone & Fax 
admin@fullstaff.org 

Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email.
From oneilb <@t> wvuhealthcare.com  Mon Sep 15 12:17:19 2014
From: oneilb <@t> wvuhealthcare.com (O'neil, Beth)
Date: Mon Sep 15 12:17:30 2014
Subject: [Histonet] RE:  Mounting media for immunofluorescence
Message-ID: <3CEB8EBCF9C7A648B9694B5696462A7140B2AFC2@NT-EX1.wvuhs.com>

We use Vectashield Mounting Medium, catalog# H-1000, Vector Laboratories, 10 ml.  My pathologists absolutely love it.  It really works for retarding photobleaching and really preserves fluorescence.

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
oneilb@wvuhealthcare.com<mailto:oneilb@wvuhealthcare.com>
Histology Supervisor, Technical Specialist
Lab:  304 - 293 - 6014
Office:  304 - 293 - 7629



From JWatson <@t> gnf.org  Mon Sep 15 12:57:24 2014
From: JWatson <@t> gnf.org (James Watson)
Date: Mon Sep 15 12:57:33 2014
Subject: [Histonet] mounting media for immunofluorescence
In-Reply-To: <D679BFF5-04BB-4754-A74B-BB867EC84F03@nsalabs.com>
References: <CANS3E9vOkzX8dtVuL=5KO18sqUrSuz-G92EV-7bHmcb1C0CSHQ@mail.gmail.com>
	<D679BFF5-04BB-4754-A74B-BB867EC84F03@nsalabs.com>
Message-ID: <A032B25305EC6949AC9AFF23C9620616C9F46D73@EX5.lj.gnf.org>

We have used a hardmount Thermo "Immumount"  Cat# 9990402.  It is the only hardmount we tested that gave good focus using the NanoZoomer scanner.  No need to seal with nail polish,  saving a lot of time.  We just re-scanned slides that were stained 6 months ago and still had great fluorescence.  We use life Technologies "Alexa Fluor" secondary antibodies.

James Watson HT? ASCP
GNF? Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel??? 858-332-4647
Fax?? 858-812-1915
jwatson@gnf.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Segovia, Claudia
Sent: Monday, September 15, 2014 8:36 AM
To: Christina Kreutzer
Cc: <histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] mounting media for immunofluorescence

Hi Christina,

We use DPX. It has worked very well for us. I am not sure about the cost though, but anything bought in kits can get really pricy. DPX comes in 500 ml.

Claudia N. Segovia
Senior Neurohistologist
Antibody Specialist
NeuroScience Associates
10915 Lake Ridge Drive
Knoxville, TN  37934
865-675-2245
csegovia@nsalabs.com

STATEMENT OF CONFIDENTIALITY:
The information contained in this electronic message and any attachments are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not the intended recipient, or the person responsible for delivering email to the intended recipient, be advised you have received this message in error and any use, dissemination, forwarding, printing or copying is strictly prohibited. Please notify NeuroScience Associates immediately at 865-675-2245 or at csegovia@nsalabs.com, and destroy all copies of this message and any attachments. You will be reimbursed for reasonable costs incurred in notifying us.

> On Sep 15, 2014, at 11:21 AM, "Christina Kreutzer" <christina.kreutzer01@gmail.com> wrote:
> 
> Hello folks,
> I am wondering which mounting medium f?r IF you are using in your 
> daily routine.
> We are using Prolong antifade gold, but it is really really really 
> expensive and I was wondering if there is a good alternative. I just 
> ordered a sample of the Dako Fluorescence Mounting Medium so we can 
> try that , but we are a little bit concerned about how long lasting 
> the fluorescence signal would be.
> 
> Well, what are your experiences so far.
> 
> All the best
> Christina
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From tony.henwood <@t> health.nsw.gov.au  Mon Sep 15 18:22:45 2014
From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN))
Date: Mon Sep 15 18:23:05 2014
Subject: [Histonet] mounting media for immunofluorescence
In-Reply-To: <CANS3E9vOkzX8dtVuL=5KO18sqUrSuz-G92EV-7bHmcb1C0CSHQ@mail.gmail.com>
References: <CANS3E9vOkzX8dtVuL=5KO18sqUrSuz-G92EV-7bHmcb1C0CSHQ@mail.gmail.com>
Message-ID: <6D6BD1DE8A5571489398B392A38A7157E01162D7@xmdb04.nch.kids>

Hi Christina,

Espada et al (2005) have shown that fluorochromes are very well preserved after dehydration (or simply air drying) followed by permanent mounting in non-aqueous synthetic media, particularly DePeX. They also found that fluorescence had not faded after one year of storage at room temperature.
(Espada, J., Juarranz, ?., Galaz, S., Ca?ete, M., Villanueva, ?., Pacheco, M., & Stockert, J. C. (2005). Non-aqueous permanent mounting for immunofluorescence microscopy. Histochemistry and cell biology, 123(3), 329-334)

This might be worth a try

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children?s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christina Kreutzer
Sent: Tuesday, 16 September 2014 1:12 AM
To: <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] mounting media for immunofluorescence

Hello folks,
I am wondering which mounting medium f?r IF you are using in your daily routine.
We are using Prolong antifade gold, but it is really really really expensive and I was wondering if there is a good alternative. I just ordered a sample of the Dako Fluorescence Mounting Medium so we can try that , but we are a little bit concerned about how long lasting the fluorescence signal would be.

Well, what are your experiences so far.

All the best
Christina
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network.

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From Timothy.Morken <@t> ucsfmedctr.org  Tue Sep 16 14:24:58 2014
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy)
Date: Tue Sep 16 14:25:11 2014
Subject: [Histonet] Control block/slide regulation question
Message-ID: <761E2B5697F795489C8710BCC72141FF3679854C@ex07.net.ucsf.edu>

Histonetters,  question came up about whether there are any regulations concerning where control blocks or slides are made. Specifically, whether a control TMA block must be made in a CLIA certified lab, as opposed to a research core facility.

I don't think there are any such regulations but would like to hear from anyone who has other knowledge. From what I know all that is required is that the TMA block/slides be validated per CLIA regulations within the CLIA -certified lab that is using them.

I've never known a vendor of controls to give any information concerning any type of FDA regulatory designation (IVD) either. So are they regulated at all? (maybe I should not even ask at the risk of giving them some bright ideas!?).

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
USA

415.514-6042  (office)
tim.morken@ucsfmedctr.org<mailto:tim.morken@ucsfmedctr.org>

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.



From amber.mckenzie <@t> gastrodocs.net  Tue Sep 16 14:59:33 2014
From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie)
Date: Tue Sep 16 14:59:37 2014
Subject: [Histonet] Symphony question
In-Reply-To: <77DD817201982748BC67D7960F2F76AF0D6274@UWHC-MBX12.uwhis.hosp.wisc.edu>
References: <6E017940-E2FA-4A97-B423-D8904C32C29B@gmail.com>
	<77DD817201982748BC67D7960F2F76AF0D6274@UWHC-MBX12.uwhis.hosp.wisc.edu>
Message-ID: <5A33C952BB67F4468AF1F36D739212BC011252ED1D@JERRY.Gia.com>

For those of you who have the Symphony, how long do you let your slides dry before filing them?  How do you keep each day separate and how much room/organization do I need?  We just got one and filed our slides after a day and now they are stuck together like a brick! Just trying to figure out the best system on this issue.  Thanks! 

From kgrobert <@t> rci.rutgers.edu  Tue Sep 16 16:04:45 2014
From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts)
Date: Tue Sep 16 16:04:50 2014
Subject: [Histonet] Slide and cassette printers?
Message-ID: <fc88267d9429736ab61eb21bbf01bccf.squirrel@webmail.rci.rutgers.edu>

What are all of you using these days?  What would be good for a small
histo lab that has the potential to grow?  (I am already aware of what
Leica and Thermo have from the archives here, but I am still interested in
your opinions, of course.)  Has anyone tried the printers, labels and
attachment machine from Brady?

Thank you all so much,
Kathleen


Principal Lab Technician
Histopathology Lab
Office of Translational Sciences
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(848) 445-1443
FAX (732) 445-6905

From joelleweaver <@t> hotmail.com  Tue Sep 16 16:18:12 2014
From: joelleweaver <@t> hotmail.com (Joelle Weaver)
Date: Tue Sep 16 16:18:17 2014
Subject: [Histonet] Control block/slide regulation question
In-Reply-To: <761E2B5697F795489C8710BCC72141FF3679854C@ex07.net.ucsf.edu>
References: <761E2B5697F795489C8710BCC72141FF3679854C@ex07.net.ucsf.edu>
Message-ID: <SNT149-W90A95194B1A9972C69036FD8C90@phx.gbl>

No regulations that I can think of that stipulate a manufacturer produced control material must have a certain FDA status for use in a clinical facility, or that the producing facility must be CLIA or GLP. I think that manufacturers want to create an impression of their product by promoting it if they are CLIA licensed or a GLP facility. I know they often use pretty stringent quality control,  with lot tracking and provide a certificate of analysis ( showing that they tested them in house and the results), with some control products. Which does show a good faith effort to establish the reliability of the control. However, since the burden of proving that any test system works in house is on the user ( CLIA & CAP method validation), not the control producer, it seems they might be off the hook for meeting a specific regulation or accreditation requirement, especially with CLIA,  since it is an old law targeting clinical laboratories. It is not that big of a leap though, and I can imagine that someone could argue that a faulty control could cause or contribute to patient risk by leading to erroneous test interpretations ( enter FDA regulations).
 
Manufacturers may not be bound to any specific legal requirements, but it seems to me that it would be bad business if over time,  their controls never worked for any lab!
 
More research oriented people may be able to comment if control material is used for FDA trials or other research, whether there is an actual legal,  regulatory or GLP requirement,  that the source or distributor of controls be CLIA, GLP or otherwise licensed or accredited for this type of work?



Joelle Weaver MAOM, HTL (ASCP) QIHC

        
  

 
> From: Timothy.Morken@ucsfmedctr.org
> To: histonet@lists.utsouthwestern.edu
> Date: Tue, 16 Sep 2014 19:24:58 +0000
> Subject: [Histonet] Control block/slide regulation question
> 
> Histonetters,  question came up about whether there are any regulations concerning where control blocks or slides are made. Specifically, whether a control TMA block must be made in a CLIA certified lab, as opposed to a research core facility.
> 
> I don't think there are any such regulations but would like to hear from anyone who has other knowledge. From what I know all that is required is that the TMA block/slides be validated per CLIA regulations within the CLIA -certified lab that is using them.
> 
> I've never known a vendor of controls to give any information concerning any type of FDA regulatory designation (IVD) either. So are they regulated at all? (maybe I should not even ask at the risk of giving them some bright ideas!?).
> 
> Tim Morken
> Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
> UC San Francisco Medical Center
> USA
> 
> 415.514-6042  (office)
> tim.morken@ucsfmedctr.org<mailto:tim.morken@ucsfmedctr.org>
> 
> CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From suetp918 <@t> comcast.net  Tue Sep 16 18:18:48 2014
From: suetp918 <@t> comcast.net (Sue)
Date: Tue Sep 16 18:19:08 2014
Subject: [Histonet] Symphony question
In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011252ED1D@JERRY.Gia.com>
References: <6E017940-E2FA-4A97-B423-D8904C32C29B@gmail.com>
	<77DD817201982748BC67D7960F2F76AF0D6274@UWHC-MBX12.uwhis.hosp.wisc.edu>
	<5A33C952BB67F4468AF1F36D739212BC011252ED1D@JERRY.Gia.com>
Message-ID: <401453667.3839887.1410909528202.JavaMail.root@comcast.net>

We had the same problems, tried oven did not work, the best plan is holding out for at least 5 days prior to filing. 
This is really not a good solution. 
From craigak12 <@t> gmail.com  Tue Sep 16 22:52:19 2014
From: craigak12 <@t> gmail.com (Jb)
Date: Tue Sep 16 22:52:33 2014
Subject: [Histonet] White Out:
Message-ID: <C26541A5-2C38-4F17-A287-695738DFF4F4@gmail.com>

" <Histonet@lists.utsouthwestern.edu>
Content-Transfer-Encoding: quoted-printable
Mime-Version: 1.0 (1.0)

Does anyone know where it says not to use white out on logs in the laborator=
y?  On logs, etc?

Thank you,

Sent from my iPhone=

From rjr6 <@t> psu.edu  Wed Sep 17 07:20:11 2014
From: rjr6 <@t> psu.edu (Roberta Horner)
Date: Wed Sep 17 07:20:16 2014
Subject: [Histonet] Slide and cassette printers?
In-Reply-To: <fc88267d9429736ab61eb21bbf01bccf.squirrel@webmail.rci.rutgers.edu>
References: <fc88267d9429736ab61eb21bbf01bccf.squirrel@webmail.rci.rutgers.edu>
Message-ID: <AECE28385C07B94C99EB6D40B823FAEA72C8CC96@CAS-EXCH-05.AG.PSU.EDU>

I use the Brady system and I like it a lot.  I work in a small animal diagnostic lab and will have 60-80 blocks on most days.  It saves me time and even better I don't have to read my writing in the morning.  I use it for the cassettes and slides mainly but I label reagents I mix with it to.  I find it very versatile.
Roberta Horner HT/HTL
Animal Diagnostic Lab
Penn State University

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Roberts
Sent: Tuesday, September 16, 2014 5:05 PM
To: histonet
Subject: [Histonet] Slide and cassette printers?

What are all of you using these days?  What would be good for a small histo lab that has the potential to grow?  (I am already aware of what Leica and Thermo have from the archives here, but I am still interested in your opinions, of course.)  Has anyone tried the printers, labels and attachment machine from Brady?

Thank you all so much,
Kathleen


Principal Lab Technician
Histopathology Lab
Office of Translational Sciences
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(848) 445-1443
FAX (732) 445-6905

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From hfedor <@t> jhmi.edu  Wed Sep 17 07:41:09 2014
From: hfedor <@t> jhmi.edu (Helen Fedor)
Date: Wed Sep 17 07:41:15 2014
Subject: [Histonet] Slide and cassette printers?
In-Reply-To: <AECE28385C07B94C99EB6D40B823FAEA72C8CC96@CAS-EXCH-05.AG.PSU.EDU>
References: <fc88267d9429736ab61eb21bbf01bccf.squirrel@webmail.rci.rutgers.edu>
	<AECE28385C07B94C99EB6D40B823FAEA72C8CC96@CAS-EXCH-05.AG.PSU.EDU>
Message-ID: <BCE07FB3A86FD040969A9EC0DD3EDA0D1747B6F2@JHEMTMWEX4.win.ad.jhu.edu>

We are also using the Brady labeler. It is versatile, easy to use, they have great tech support. We are not using the cassette label attaching machine. For the cassette printer we are using the Primera. The Tech support there is also great.


Helen

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Wednesday, September 17, 2014 8:20 AM
To: Kathleen Roberts; histonet
Subject: RE: [Histonet] Slide and cassette printers?

I use the Brady system and I like it a lot.  I work in a small animal diagnostic lab and will have 60-80 blocks on most days.  It saves me time and even better I don't have to read my writing in the morning.  I use it for the cassettes and slides mainly but I label reagents I mix with it to.  I find it very versatile.
Roberta Horner HT/HTL
Animal Diagnostic Lab
Penn State University

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Roberts
Sent: Tuesday, September 16, 2014 5:05 PM
To: histonet
Subject: [Histonet] Slide and cassette printers?

What are all of you using these days?  What would be good for a small histo lab that has the potential to grow?  (I am already aware of what Leica and Thermo have from the archives here, but I am still interested in your opinions, of course.)  Has anyone tried the printers, labels and attachment machine from Brady?

Thank you all so much,
Kathleen


Principal Lab Technician
Histopathology Lab
Office of Translational Sciences
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(848) 445-1443
FAX (732) 445-6905

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From rsrichmond <@t> gmail.com  Wed Sep 17 08:08:32 2014
From: rsrichmond <@t> gmail.com (Bob Richmond)
Date: Wed Sep 17 08:08:38 2014
Subject: [Histonet] Histotechnology certification for cytotechnologists
Message-ID: <CAOKsRH4fXNp+HfG-LSoBVbiQbNaP_V0tpzvG5vUHascceiPB6g@mail.gmail.com>

With declining utilization of gynecologic cytology ("Pap smears")
opportunities for cytotechnologists are decreasing, and some of them are
considering adding histotechnology to their skills and certifications. One
small hospital's pathology service I'm acquainted with is trying to retain
its cytotechnologist by cross-training her as a histotechnologist.

Certification as a cytotechnologist requires all of the course work needed
for histotechnologist certification, so that isn't a problem. And
cytopreparation requires some of the needed skills for histotechnology -
though obviously not the primary skill of cutting paraffin. What are the
requirements for a cytotechnologist to sit the registry examination for a
histotechnologist? Is the National Society for Histotechnology (NSH)
considering addressing this issue?

Bob Richmond
Samurai Pathologist
Maryville TN
From wbenton <@t> cua.md  Wed Sep 17 08:16:47 2014
From: wbenton <@t> cua.md (Walter Benton)
Date: Wed Sep 17 08:19:52 2014
Subject: [Histonet] Histotechnology certification for cytotechnologists
In-Reply-To: <CAOKsRH4fXNp+HfG-LSoBVbiQbNaP_V0tpzvG5vUHascceiPB6g@mail.gmail.com>
References: <CAOKsRH4fXNp+HfG-LSoBVbiQbNaP_V0tpzvG5vUHascceiPB6g@mail.gmail.com>
Message-ID: <0B8979A204680A42B93A52B486088CD93938636C72@CUAEXH1.GCU-MD.local>

Bob,



Here are the requirements straight from the ASCP/BOC

http://ascp.org/Board-of-Certification/GetCertified#tabs-1



For Histotechnician:

Application Fee: $200

To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes:

Route 1: Successful completion of a NAACLS accredited Histotechnician program within the last 5 years prior to the date of application for examination; OR

Route 2: At least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry (must include credit hours in both), or an associate degree from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry(must include credit hours in both), AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years.

*CMS CLIA certificate of registration, compliance, accreditation; AND/OR
CAP, AABB, Joint Commission accreditation; OR
JCI accreditation; OR
Accreditation under ISO 15189.

Laboratory Experience
To fulfill the experience requirement for the Histotechnician examination, you must have experience within the last ten years in ALL of the following areas:

 *   Fixation
 *   Microtomy
 *   Processing
 *   Staining

For Histotechnologist:



Application Fee: $225

To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes:

Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry (must include credit hours in both), AND successful completion of a NAACLS accredited Histotechnician or Histotechnology program within the last 5 years; OR

Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry (must include credit hours in both), AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years.

*CMS CLIA certificate of registration, compliance, accreditation; AND/OR
CAP, AABB, Joint Commission accreditation; OR
JCI accreditation; OR
Accreditation under ISO 15189.

Clinical Laboratory Experience
To fulfill the experience requirement for the Histotechnologist examination, you must have experience within the last ten years in ALL of the following areas:

 *   Fixation
 *   Microtomy
 *   Processing
 *   Staining

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond [rsrichmond@gmail.com]
Sent: Wednesday, September 17, 2014 9:08 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histotechnology certification for cytotechnologists

With declining utilization of gynecologic cytology ("Pap smears")
opportunities for cytotechnologists are decreasing, and some of them are
considering adding histotechnology to their skills and certifications. One
small hospital's pathology service I'm acquainted with is trying to retain
its cytotechnologist by cross-training her as a histotechnologist.

Certification as a cytotechnologist requires all of the course work needed
for histotechnologist certification, so that isn't a problem. And
cytopreparation requires some of the needed skills for histotechnology -
though obviously not the primary skill of cutting paraffin. What are the
requirements for a cytotechnologist to sit the registry examination for a
histotechnologist? Is the National Society for Histotechnology (NSH)
considering addressing this issue?

Bob Richmond
Samurai Pathologist
Maryville TN
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

________________________________
CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy.
From liz <@t> premierlab.com  Wed Sep 17 08:20:51 2014
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Wed Sep 17 08:20:55 2014
Subject: [Histonet] White Out:
In-Reply-To: <C26541A5-2C38-4F17-A287-695738DFF4F4@gmail.com>
References: <C26541A5-2C38-4F17-A287-695738DFF4F4@gmail.com>
Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E021EC@SBS2K8.premierlab.local>

It may not state it directly in either CAP or GLP guidelines but in a GLP compliant lab it is considered an industry standard not to use white out.  As a GLP compliant lab we have an entire SOP devoted to data entry.

If an error is made when data is being recorded or you notice an error at a later date when the form is being reviewed, even if it is on a QC checklist there are specific procedures that are followed.  You can not scribble over or abliterate what was written, you need to line through the error, make the correction and then explain why the form has been corrected, initial and date.  We use a series of codes one of them being RE - recording error.  We have these codes posted all over the lab as a form so individuals will use the codes and record data properly.

I would have to say from experience that the biggest problem we encounter with data entry is scribbling over a recording error because that is a habit we all have and it does take some training to educate individuals in correct data entry.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
liz@premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb [craigak12@gmail.com]
Sent: Tuesday, September 16, 2014 9:52 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] White Out:

" <Histonet@lists.utsouthwestern.edu>
Content-Transfer-Encoding: quoted-printable
Mime-Version: 1.0 (1.0)

Does anyone know where it says not to use white out on logs in the laborator=
y?  On logs, etc?

Thank you,

Sent from my iPhone=

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From liz <@t> premierlab.com  Wed Sep 17 08:21:31 2014
From: liz <@t> premierlab.com (Elizabeth Chlipala)
Date: Wed Sep 17 08:26:13 2014
Subject: [Histonet] Histotechnology certification for cytotechnologists
In-Reply-To: <CAOKsRH4fXNp+HfG-LSoBVbiQbNaP_V0tpzvG5vUHascceiPB6g@mail.gmail.com>
References: <CAOKsRH4fXNp+HfG-LSoBVbiQbNaP_V0tpzvG5vUHascceiPB6g@mail.gmail.com>
Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E021ED@SBS2K8.premierlab.local>

Bob

I believe this is correct, but others out there that may know more please correct me if I am incorrect.

If they have a BS they would fall in that category of eligibiity.  I would think most cytotechs do since they needed this to sit for the cytology exam.  They need to have the proper college courses in science, etc.  The ASCP has this listed on their website then after one year of training and experinence in a histology lab they would be eligible to sit for the registry.

The route in BS or BA with one year on the job training.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
liz@premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond [rsrichmond@gmail.com]
Sent: Wednesday, September 17, 2014 7:08 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histotechnology certification for cytotechnologists

With declining utilization of gynecologic cytology ("Pap smears")
opportunities for cytotechnologists are decreasing, and some of them are
considering adding histotechnology to their skills and certifications. One
small hospital's pathology service I'm acquainted with is trying to retain
its cytotechnologist by cross-training her as a histotechnologist.

Certification as a cytotechnologist requires all of the course work needed
for histotechnologist certification, so that isn't a problem. And
cytopreparation requires some of the needed skills for histotechnology -
though obviously not the primary skill of cutting paraffin. What are the
requirements for a cytotechnologist to sit the registry examination for a
histotechnologist? Is the National Society for Histotechnology (NSH)
considering addressing this issue?

Bob Richmond
Samurai Pathologist
Maryville TN
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From Jenn.Sands <@t> EssentiaHealth.org  Wed Sep 17 08:46:38 2014
From: Jenn.Sands <@t> EssentiaHealth.org (Sands, Jenn)
Date: Wed Sep 17 08:46:49 2014
Subject: [Histonet] Histotechnology certification for cytotechnologists
In-Reply-To: <0B8979A204680A42B93A52B486088CD93938636C72@CUAEXH1.GCU-MD.local>
References: <CAOKsRH4fXNp+HfG-LSoBVbiQbNaP_V0tpzvG5vUHascceiPB6g@mail.gmail.com>
	<0B8979A204680A42B93A52B486088CD93938636C72@CUAEXH1.GCU-MD.local>
Message-ID: <389C7D597C19C94D9E6A51F6CAC225F35282DB5B@skimmer.medcampus.org>

I personally obtained my HTL through this method and it was successful!  I have several interested in completing this same process.

Jenn Sands
Anatomic Pathology Supervisor
Essentia Health
South University Clinic
Department of Laboratory Medicine and Pathology
1702 South University Drive, Fargo, ND 58103
P: 701-364-3245 | F: 701-364-8737 | Jenn.Sands@essentiahealth.org 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton
Sent: Wednesday, September 17, 2014 8:17 AM
To: Bob Richmond; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Histotechnology certification for cytotechnologists

Bob,



Here are the requirements straight from the ASCP/BOC

http://ascp.org/Board-of-Certification/GetCertified#tabs-1



For Histotechnician:

Application Fee: $200

To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes:

Route 1: Successful completion of a NAACLS accredited Histotechnician program within the last 5 years prior to the date of application for examination; OR

Route 2: At least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry (must include credit hours in both), or an associate degree from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry(must include credit hours in both), AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years.

*CMS CLIA certificate of registration, compliance, accreditation; AND/OR CAP, AABB, Joint Commission accreditation; OR JCI accreditation; OR Accreditation under ISO 15189.

Laboratory Experience
To fulfill the experience requirement for the Histotechnician examination, you must have experience within the last ten years in ALL of the following areas:

 *   Fixation
 *   Microtomy
 *   Processing
 *   Staining

For Histotechnologist:



Application Fee: $225

To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes:

Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry (must include credit hours in both), AND successful completion of a NAACLS accredited Histotechnician or Histotechnology program within the last 5 years; OR

Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry (must include credit hours in both), AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years.

*CMS CLIA certificate of registration, compliance, accreditation; AND/OR CAP, AABB, Joint Commission accreditation; OR JCI accreditation; OR Accreditation under ISO 15189.

Clinical Laboratory Experience
To fulfill the experience requirement for the Histotechnologist examination, you must have experience within the last ten years in ALL of the following areas:

 *   Fixation
 *   Microtomy
 *   Processing
 *   Staining

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond [rsrichmond@gmail.com]
Sent: Wednesday, September 17, 2014 9:08 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histotechnology certification for cytotechnologists

With declining utilization of gynecologic cytology ("Pap smears") opportunities for cytotechnologists are decreasing, and some of them are considering adding histotechnology to their skills and certifications. One small hospital's pathology service I'm acquainted with is trying to retain its cytotechnologist by cross-training her as a histotechnologist.

Certification as a cytotechnologist requires all of the course work needed for histotechnologist certification, so that isn't a problem. And cytopreparation requires some of the needed skills for histotechnology - though obviously not the primary skill of cutting paraffin. What are the requirements for a cytotechnologist to sit the registry examination for a histotechnologist? Is the National Society for Histotechnology (NSH) considering addressing this issue?

Bob Richmond
Samurai Pathologist
Maryville TN
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From b427297 <@t> aol.com  Wed Sep 17 10:38:00 2014
From: b427297 <@t> aol.com (William J. O'Connor III)
Date: Wed Sep 17 10:38:32 2014
Subject: [Histonet] Whole rat head histology
In-Reply-To: <389C7D597C19C94D9E6A51F6CAC225F35282DB5B@skimmer.medcampus.org>
References: <CAOKsRH4fXNp+HfG-LSoBVbiQbNaP_V0tpzvG5vUHascceiPB6g@mail.gmail.com>
	<0B8979A204680A42B93A52B486088CD93938636C72@CUAEXH1.GCU-MD.local>
	<389C7D597C19C94D9E6A51F6CAC225F35282DB5B@skimmer.medcampus.org>
Message-ID: <8D1A0A47F163FC8-1464-170E@webmail-vm090.sysops.aol.com>


I have a fun project - processing a whole rat head, skull with brain, for serial sections to see a brain/skull congenital deformity.    Anyone have any experience with this?  I'd love it if you could share your protocol.  Paraffin or plastic?
Jackie O'

From PAMarcum <@t> uams.edu  Wed Sep 17 11:01:36 2014
From: PAMarcum <@t> uams.edu (Marcum, Pamela A)
Date: Wed Sep 17 11:01:56 2014
Subject: [Histonet] Whole rat head histology
In-Reply-To: <8D1A0A47F163FC8-1464-170E@webmail-vm090.sysops.aol.com>
References: <CAOKsRH4fXNp+HfG-LSoBVbiQbNaP_V0tpzvG5vUHascceiPB6g@mail.gmail.com>
	<0B8979A204680A42B93A52B486088CD93938636C72@CUAEXH1.GCU-MD.local>
	<389C7D597C19C94D9E6A51F6CAC225F35282DB5B@skimmer.medcampus.org>
	<8D1A0A47F163FC8-1464-170E@webmail-vm090.sysops.aol.com>
Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32012526AA1F@Mail2Node2.ad.uams.edu>

How old are the rats?  

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of William J. O'Connor III
Sent: Wednesday, September 17, 2014 10:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Whole rat head histology


I have a fun project - processing a whole rat head, skull with brain, for serial sections to see a brain/skull congenital deformity.    Anyone have any experience with this?  I'd love it if you could share your protocol.  Paraffin or plastic?
Jackie O'


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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From Sarah.Dysart <@t> stdavids.com  Wed Sep 17 11:13:14 2014
From: Sarah.Dysart <@t> stdavids.com (Sarah.Dysart@stdavids.com)
Date: Wed Sep 17 11:13:31 2014
Subject: [Histonet] Automated Special Stainer...
Message-ID: <34C22BB94729434598D767D3F4EB95E0E2174B0D69@FWDCWPMSGCMS03.hca.corpad.net>

Opinions on Ventana versus Dako...Go!

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP)
Pathology Supervisor
St. David's North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

From suetp918 <@t> comcast.net  Wed Sep 17 11:30:18 2014
From: suetp918 <@t> comcast.net (Sue)
Date: Wed Sep 17 11:30:38 2014
Subject: [Histonet] Automated Special Stainer...
In-Reply-To: <34C22BB94729434598D767D3F4EB95E0E2174B0D69@FWDCWPMSGCMS03.hca.corpad.net>
References: <34C22BB94729434598D767D3F4EB95E0E2174B0D69@FWDCWPMSGCMS03.hca.corpad.net>
Message-ID: <81116639.3933059.1410971418142.JavaMail.root@comcast.net>

Have Ventana like it but cost is high, ave no experience with new DAKO but old platform was nice 
? 

Sue Paturzo 
From TanyaAbbott <@t> catholichealth.net  Wed Sep 17 11:48:02 2014
From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya)
Date: Wed Sep 17 11:48:08 2014
Subject: [Histonet] Rapid on site evaluation for EBUS
Message-ID: <852F7D2C14FB464D80E182B15DB138AF394D2F01@CHIEX005.CHI.catholichealth.net>

This is possibly more of a Cytology question, but we are a small Path lab that covers it all!! We just did our first EBUS, and are wondering how to code for the Rapid on site evaluations, any suggestions?
Thanks! Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabbott@catholichealth.net

This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.
From Nancy_Schmitt <@t> pa-ucl.com  Wed Sep 17 12:31:12 2014
From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt)
Date: Wed Sep 17 12:31:27 2014
Subject: [Histonet] Slide and cassette printers
Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115909515@PEITHA.wad.pa-ucl.com>

We are using cassette and slide labeling system from General Data - like it a lot!



Nancy Schmitt MLT, HT(ASCP)

United Clinical Laboratories

Dubuque, IA



Message: 3

Date: Tue, 16 Sep 2014 17:04:45 -0400 (EDT)

From: "Kathleen Roberts" <kgrobert@rci.rutgers.edu<mailto:kgrobert@rci.rutgers.edu>>

Subject: [Histonet] Slide and cassette printers?

To: "histonet" <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>>

Message-ID:

      <fc88267d9429736ab61eb21bbf01bccf.squirrel@webmail.rci.rutgers.edu<mailto:fc88267d9429736ab61eb21bbf01bccf.squirrel@webmail.rci.rutgers.edu>>

Content-Type: text/plain;charset=iso-8859-1



What are all of you using these days?  What would be good for a small histo lab that has the potential to grow?  (I am already aware of what Leica and Thermo have from the archives here, but I am still interested in your opinions, of course.)  Has anyone tried the printers, labels and attachment machine from Brady?



Thank you all so much,

Kathleen







NOTICE: This email may contain legally privileged information. The information
is for the use of only the intended recipient(s) even if addressed
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From bcooper <@t> chla.usc.edu  Wed Sep 17 12:32:33 2014
From: bcooper <@t> chla.usc.edu (Cooper, Brian)
Date: Wed Sep 17 12:32:39 2014
Subject: [Histonet] RE: Automated Special Stainer...
In-Reply-To: <34C22BB94729434598D767D3F4EB95E0E2174B0D69@FWDCWPMSGCMS03.hca.corpad.net>
References: <34C22BB94729434598D767D3F4EB95E0E2174B0D69@FWDCWPMSGCMS03.hca.corpad.net>
Message-ID: <C12647AD4408834C8AB48FB0389C63E396BFA495@CHLAEXMBH01.LA.AD.CHLA.ORG>

Dako Artisan all the way!!!!!   I've used both systems (the Artisan in my old institution).  We currently have a few of the new Benchmarks on demo right now.  Thus far, we're not that impressed.  Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan.  Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab).   Oh yeah, one more thing.  The reagent tray capacity on the Artisan is about twice what it is on a Benchmark.  You'll need two Benchmarks to do what one Artisan does, TATs being equal.         

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcooper@chla.usc.edu 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah.Dysart@stdavids.com
Sent: Wednesday, September 17, 2014 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Automated Special Stainer...

Opinions on Ventana versus Dako...Go!

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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or distribution is prohibited. If you are not the intended recipient, please
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From PAMarcum <@t> uams.edu  Wed Sep 17 12:44:35 2014
From: PAMarcum <@t> uams.edu (Marcum, Pamela A)
Date: Wed Sep 17 12:44:42 2014
Subject: [Histonet] RE: Automated Special Stainer...
In-Reply-To: <C12647AD4408834C8AB48FB0389C63E396BFA495@CHLAEXMBH01.LA.AD.CHLA.ORG>
References: <34C22BB94729434598D767D3F4EB95E0E2174B0D69@FWDCWPMSGCMS03.hca.corpad.net>
	<C12647AD4408834C8AB48FB0389C63E396BFA495@CHLAEXMBH01.LA.AD.CHLA.ORG>
Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32012526AAA5@Mail2Node2.ad.uams.edu>

We love our Dako Artisan stainers and would not take the other brand back for any reason.  The techs love the ease of use and the pathologist love the stains.  What more could you ask!!  

Pam Marcum

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Wednesday, September 17, 2014 12:33 PM
To: Sarah.Dysart@stdavids.com; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Automated Special Stainer...

Dako Artisan all the way!!!!!   I've used both systems (the Artisan in my old institution).  We currently have a few of the new Benchmarks on demo right now.  Thus far, we're not that impressed.  Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan.  Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab).   Oh yeah, one more thing.  The reagent tray capacity on the Artisan is about twice what it is on a Benchmark.  You'll need two Benchmarks to do what one Artisan does, TATs being equal.         

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper@chla.usc.edu 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah.Dysart@stdavids.com
Sent: Wednesday, September 17, 2014 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Automated Special Stainer...

Opinions on Ventana versus Dako...Go!

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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From Timothy.Morken <@t> ucsfmedctr.org  Wed Sep 17 12:58:02 2014
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy)
Date: Wed Sep 17 12:58:10 2014
Subject: [Histonet] RE: Automated Special Stainer...
In-Reply-To: <C12647AD4408834C8AB48FB0389C63E396BFA495@CHLAEXMBH01.LA.AD.CHLA.ORG>
References: <34C22BB94729434598D767D3F4EB95E0E2174B0D69@FWDCWPMSGCMS03.hca.corpad.net>
	<C12647AD4408834C8AB48FB0389C63E396BFA495@CHLAEXMBH01.LA.AD.CHLA.ORG>
Message-ID: <761E2B5697F795489C8710BCC72141FF36799252@ex07.net.ucsf.edu>

Brian wrote: "... one more thing.  The reagent tray capacity on the Artisan is about twice what it is on a Benchmark."

Are the TAT's equal? When we loaded up our old artisan with slides (50) and full of reagents it took over 5 hours to finish - way past our deadline. So nobody wanted to use it that way so the techs resisted and wanted to do manual to get the slide out. We ended up putting only a few stains on it.

We  are looking at doing specials the way we do immunos - on demand all day rather than in one batch in the AM. 

It seemed to me that the 20-slide units from Ventana allow small batches and run in parallel or allow staggered use. Buying a 50-slide capacity instrument and then putting only 20 slides at a time on it seems odd somehow.


We looking at each of these as well and not sure yet which would serve us better. We do about 60-80 specials slides per day, and 13 stains give us 90% of our volume. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Wednesday, September 17, 2014 10:33 AM
To: Sarah.Dysart@stdavids.com; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Automated Special Stainer...

Dako Artisan all the way!!!!!   I've used both systems (the Artisan in my old institution).  We currently have a few of the new Benchmarks on demo right now.  Thus far, we're not that impressed.  Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan.  Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab).   Oh yeah, one more thing.  The reagent tray capacity on the Artisan is about twice what it is on a Benchmark.  You'll need two Benchmarks to do what one Artisan does, TATs being equal.         

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper@chla.usc.edu 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah.Dysart@stdavids.com
Sent: Wednesday, September 17, 2014 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Automated Special Stainer...

Opinions on Ventana versus Dako...Go!

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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From Joyce.Weems <@t> emoryhealthcare.org  Wed Sep 17 13:06:09 2014
From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.)
Date: Wed Sep 17 13:06:20 2014
Subject: [Histonet] RE: Automated Special Stainer...
In-Reply-To: <761E2B5697F795489C8710BCC72141FF36799252@ex07.net.ucsf.edu>
References: <34C22BB94729434598D767D3F4EB95E0E2174B0D69@FWDCWPMSGCMS03.hca.corpad.net>
	<C12647AD4408834C8AB48FB0389C63E396BFA495@CHLAEXMBH01.LA.AD.CHLA.ORG>
	<761E2B5697F795489C8710BCC72141FF36799252@ex07.net.ucsf.edu>
Message-ID: <E3A4EBD57A691646BCCED4AA5911A030011C343402@e14mbx12n.Enterprise.emory.net>

I highly recommend the Leica Bond III. Company is excellent to work with and reagent rental a perfect solution to no capital. On line retrieval - 3 trays of 10 slides each that can be started as needed.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s).  It may contain information that is privileged and confidential.  Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, September 17, 2014 1:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Automated Special Stainer...

Brian wrote: "... one more thing.  The reagent tray capacity on the Artisan is about twice what it is on a Benchmark."

Are the TAT's equal? When we loaded up our old artisan with slides (50) and full of reagents it took over 5 hours to finish - way past our deadline. So nobody wanted to use it that way so the techs resisted and wanted to do manual to get the slide out. We ended up putting only a few stains on it.

We  are looking at doing specials the way we do immunos - on demand all day rather than in one batch in the AM.

It seemed to me that the 20-slide units from Ventana allow small batches and run in parallel or allow staggered use. Buying a 50-slide capacity instrument and then putting only 20 slides at a time on it seems odd somehow.


We looking at each of these as well and not sure yet which would serve us better. We do about 60-80 specials slides per day, and 13 stains give us 90% of our volume.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Wednesday, September 17, 2014 10:33 AM
To: Sarah.Dysart@stdavids.com; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Automated Special Stainer...

Dako Artisan all the way!!!!!   I've used both systems (the Artisan in my old institution).  We currently have a few of the new Benchmarks on demo right now.  Thus far, we're not that impressed.  Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan.  Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab).   Oh yeah, one more thing.  The reagent tray capacity on the Artisan is about twice what it is on a Benchmark.  You'll need two Benchmarks to do what one Artisan does, TATs being equal.

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper@chla.usc.edu


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah.Dysart@stdavids.com
Sent: Wednesday, September 17, 2014 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Automated Special Stainer...

Opinions on Ventana versus Dako...Go!

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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From mucram11 <@t> comcast.net  Wed Sep 17 13:08:26 2014
From: mucram11 <@t> comcast.net (Pam Marcum)
Date: Wed Sep 17 13:08:51 2014
Subject: [Histonet] RE: Automated Special Stainer...
In-Reply-To: <761E2B5697F795489C8710BCC72141FF36799252@ex07.net.ucsf.edu>
References: <34C22BB94729434598D767D3F4EB95E0E2174B0D69@FWDCWPMSGCMS03.hca.corpad.net>
	<C12647AD4408834C8AB48FB0389C63E396BFA495@CHLAEXMBH01.LA.AD.CHLA.ORG>
	<761E2B5697F795489C8710BCC72141FF36799252@ex07.net.ucsf.edu>
Message-ID: <683122955.7539983.1410977306143.JavaMail.root@comcast.net>

I understand the problem.? We have two Artisans and rarely have a time issue.? 

----- Original Message -----

From: "Timothy Morken" <Timothy.Morken@ucsfmedctr.org> 
To: "Histonet" <histonet@lists.utsouthwestern.edu> 
Sent: Wednesday, September 17, 2014 12:58:02 PM 
Subject: [Histonet] RE: Automated Special Stainer... 

Brian wrote: "... one more thing. ?The reagent tray capacity on the Artisan is about twice what it is on a Benchmark." 

Are the TAT's equal? When we loaded up our old artisan with slides (50) and full of reagents it took over 5 hours to finish - way past our deadline. So nobody wanted to use it that way so the techs resisted and wanted to do manual to get the slide out. We ended up putting only a few stains on it. 

We ?are looking at doing specials the way we do immunos - on demand all day rather than in one batch in the AM. 

It seemed to me that the 20-slide units from Ventana allow small batches and run in parallel or allow staggered use. Buying a 50-slide capacity instrument and then putting only 20 slides at a time on it seems odd somehow. 


We looking at each of these as well and not sure yet which would serve us better. We do about 60-80 specials slides per day, and 13 stains give us 90% of our volume. 

Tim Morken 
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies 
UC San Francisco Medical Center 
San Francisco, CA 

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. 


-----Original Message----- 
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian 
Sent: Wednesday, September 17, 2014 10:33 AM 
To: Sarah.Dysart@stdavids.com; histonet@lists.utsouthwestern.edu 
Subject: [Histonet] RE: Automated Special Stainer... 

Dako Artisan all the way!!!!! ? I've used both systems (the Artisan in my old institution). ?We currently have a few of the new Benchmarks on demo right now. ?Thus far, we're not that impressed. ?Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan. ?Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab). ? Oh yeah, one more thing. ?The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. ?You'll need two Benchmarks to do what one Artisan does, TATs being equal. ? ? ? ? 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper@chla.usc.edu 


-----Original Message----- 
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah.Dysart@stdavids.com 
Sent: Wednesday, September 17, 2014 9:13 AM 
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Automated Special Stainer... 

Opinions on Ventana versus Dako...Go! 

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 
12221 North Mopac Expressway 
Austin, Texas ?78758 
(512)901-1220 

_______________________________________________ 
Histonet mailing list 
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



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From twebster <@t> CRH.org  Wed Sep 17 14:12:49 2014
From: twebster <@t> CRH.org (Webster, Thomas S.)
Date: Wed Sep 17 14:12:57 2014
Subject: [Histonet] Rapid on site evaluation for EBUS
Message-ID: <7207186ED68FB542803CAF1CE6E82FF80871B00F@exmb1.crh.org>

It depends. Tell more about the procedure. Pathologist present giving adequacy?  Number of passes evaluated? Each pass received separately and evaluated or were some passes grouped together? Did you receive needle aspirations or touch preps or a combination of the two?


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Columbus Regional Hospital
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Columbus, Indiana 47201
From tbraud <@t> holyredeemer.com  Wed Sep 17 14:19:14 2014
From: tbraud <@t> holyredeemer.com (Terri  Braud)
Date: Wed Sep 17 14:19:18 2014
Subject: [Histonet] RE: White Out
In-Reply-To: <20140917161149.D3EA11E8083@trendmess-svr.holyredeemer.local>
References: <20140917161149.D3EA11E8083@trendmess-svr.holyredeemer.local>
Message-ID: <BFBF7C6B9627B947B74CBEBD45CE368B143B2BE8@hrex-svr.holyredeemer.local>

Concerning the use of White Out on anything considered part of the
patient's permanent record.  This actually is LEGISLATION.  It is
specifically mentioned in many state codes, and is part of the federal
laws concerning medicare and medicaid fraud. See reference below.
Medicare program Integrity Manual Chapter 4.
www.cms.gov/Regulations-and-Guidance
Apr 11, 2003 ... 4.8.2 - Production of Medical Records and Documentation
for an ....not limited to: obliterated sections, missing pages, inserted
pages, white out, and...

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676

Today's Topics:
   6. White Out: (Jb)
  11. RE: White Out: (Elizabeth Chlipala)


Message: 6
Date: Tue, 16 Sep 2014 20:52:19 -0700
From: Jb <craigak12@gmail.com>
Subject: [Histonet] White Out:
Does anyone know where it says not to use white out on logs in the
laborator= y?  On logs, etc?

Thank you,
Sent from my iPhone=
------------------------------

Message: 11
Date: Wed, 17 Sep 2014 07:20:51 -0600
From: Elizabeth Chlipala <liz@premierlab.com>
Subject: RE: [Histonet] White Out:
It may not state it directly in either CAP or GLP guidelines but in a
GLP compliant lab it is considered an industry standard not to use white
out.  As a GLP compliant lab we have an entire SOP devoted to data
entry.

---------------------------------------------------------------------------------



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From bcooper <@t> chla.usc.edu  Wed Sep 17 14:36:55 2014
From: bcooper <@t> chla.usc.edu (Cooper, Brian)
Date: Wed Sep 17 14:37:00 2014
Subject: [Histonet] RE: Automated Special Stainer...
In-Reply-To: <761E2B5697F795489C8710BCC72141FF36799252@ex07.net.ucsf.edu>
References: <34C22BB94729434598D767D3F4EB95E0E2174B0D69@FWDCWPMSGCMS03.hca.corpad.net>
	<C12647AD4408834C8AB48FB0389C63E396BFA495@CHLAEXMBH01.LA.AD.CHLA.ORG>
	<761E2B5697F795489C8710BCC72141FF36799252@ex07.net.ucsf.edu>
Message-ID: <C12647AD4408834C8AB48FB0389C63E396BFA7F0@CHLAEXMBH01.LA.AD.CHLA.ORG>

I guess "TAT's being equal" is a relative term, depending upon your workload.  For our purposes, it's not slide capacity that poses a problem--it's reagent capacity.  We've never loaded 50 special stains at any one time--we typically don't even have half that amount in one day!  

So here's a scenario common to the workload at our institution.  For our liver panels, we run Iron, Trichrome, Retic, PAS with and without Digestion.  That pretty much maxes out the reagent capacity on the Benchmark.  Sure, we can load up to 20 of these onto the Benchmark.  But as is so frequently the case, we also have just 1 GMS that also needs to be stained!  This is where the second Benchmark comes into play, or we'll have to stain by hand.   One Artisan can handle this at the same time, and if memory serves (disclosure--it's been about 2 years, and the model I used didn't have online deparaffinization), it didn't add all that much time to the process.  It was certainly faster than waiting for the machine to complete, and start another run.   For our institution, it's either going to be 2 Artisans or 2 Benchmarks.  The Artisans will give us some greater flexibility due to the greater reagent capacity.  

Brian


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, September 17, 2014 10:58 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Automated Special Stainer...

Brian wrote: "... one more thing.  The reagent tray capacity on the Artisan is about twice what it is on a Benchmark."

Are the TAT's equal? When we loaded up our old artisan with slides (50) and full of reagents it took over 5 hours to finish - way past our deadline. So nobody wanted to use it that way so the techs resisted and wanted to do manual to get the slide out. We ended up putting only a few stains on it.

We  are looking at doing specials the way we do immunos - on demand all day rather than in one batch in the AM. 

It seemed to me that the 20-slide units from Ventana allow small batches and run in parallel or allow staggered use. Buying a 50-slide capacity instrument and then putting only 20 slides at a time on it seems odd somehow.


We looking at each of these as well and not sure yet which would serve us better. We do about 60-80 specials slides per day, and 13 stains give us 90% of our volume. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Wednesday, September 17, 2014 10:33 AM
To: Sarah.Dysart@stdavids.com; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Automated Special Stainer...

Dako Artisan all the way!!!!!   I've used both systems (the Artisan in my old institution).  We currently have a few of the new Benchmarks on demo right now.  Thus far, we're not that impressed.  Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan.  Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab).   Oh yeah, one more thing.  The reagent tray capacity on the Artisan is about twice what it is on a Benchmark.  You'll need two Benchmarks to do what one Artisan does, TATs being equal.         

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper@chla.usc.edu 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah.Dysart@stdavids.com
Sent: Wednesday, September 17, 2014 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Automated Special Stainer...

Opinions on Ventana versus Dako...Go!

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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From LSebree <@t> uwhealth.org  Wed Sep 17 14:38:00 2014
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Wed Sep 17 14:38:05 2014
Subject: [Histonet] RE: Automated Special Stainer...
In-Reply-To: <761E2B5697F795489C8710BCC72141FF36799252@ex07.net.ucsf.edu>
References: <34C22BB94729434598D767D3F4EB95E0E2174B0D69@FWDCWPMSGCMS03.hca.corpad.net>
	<C12647AD4408834C8AB48FB0389C63E396BFA495@CHLAEXMBH01.LA.AD.CHLA.ORG>,
	<761E2B5697F795489C8710BCC72141FF36799252@ex07.net.ucsf.edu>
Message-ID: <77DD817201982748BC67D7960F2F76AF0DBD92@UWHC-MBX12.uwhis.hosp.wisc.edu>

We've had VMS instruments from day 1 and currently have 4 Ultras.  The random access capability is awesome and we can even get out EBER ISH slides the same day if put on in the morning.  Our HER2 dual ISH stains run overnight as they are 14 hour protocols.  Reproducibility among the 4 instruments and concurrent/consecutive stains on the same instrument are a given.  Technical advice and service are outstanding.  And, no, I do not have a stake in the company just am really sold by their products.


Linda A. Sebree
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org]
Sent: Wednesday, September 17, 2014 12:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Automated Special Stainer...

Brian wrote: "... one more thing.  The reagent tray capacity on the Artisan is about twice what it is on a Benchmark."

Are the TAT's equal? When we loaded up our old artisan with slides (50) and full of reagents it took over 5 hours to finish - way past our deadline. So nobody wanted to use it that way so the techs resisted and wanted to do manual to get the slide out. We ended up putting only a few stains on it.

We  are looking at doing specials the way we do immunos - on demand all day rather than in one batch in the AM.

It seemed to me that the 20-slide units from Ventana allow small batches and run in parallel or allow staggered use. Buying a 50-slide capacity instrument and then putting only 20 slides at a time on it seems odd somehow.


We looking at each of these as well and not sure yet which would serve us better. We do about 60-80 specials slides per day, and 13 stains give us 90% of our volume.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Wednesday, September 17, 2014 10:33 AM
To: Sarah.Dysart@stdavids.com; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Automated Special Stainer...

Dako Artisan all the way!!!!!   I've used both systems (the Artisan in my old institution).  We currently have a few of the new Benchmarks on demo right now.  Thus far, we're not that impressed.  Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan.  Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab).   Oh yeah, one more thing.  The reagent tray capacity on the Artisan is about twice what it is on a Benchmark.  You'll need two Benchmarks to do what one Artisan does, TATs being equal.

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper@chla.usc.edu


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah.Dysart@stdavids.com
Sent: Wednesday, September 17, 2014 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Automated Special Stainer...

Opinions on Ventana versus Dako...Go!

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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From LSebree <@t> uwhealth.org  Wed Sep 17 14:45:08 2014
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Wed Sep 17 14:45:16 2014
Subject: [Histonet] RE: Automated Special Stainer...
In-Reply-To: <C12647AD4408834C8AB48FB0389C63E396BFA7F0@CHLAEXMBH01.LA.AD.CHLA.ORG>
References: <34C22BB94729434598D767D3F4EB95E0E2174B0D69@FWDCWPMSGCMS03.hca.corpad.net>
	<C12647AD4408834C8AB48FB0389C63E396BFA495@CHLAEXMBH01.LA.AD.CHLA.ORG>
	<761E2B5697F795489C8710BCC72141FF36799252@ex07.net.ucsf.edu>,
	<C12647AD4408834C8AB48FB0389C63E396BFA7F0@CHLAEXMBH01.LA.AD.CHLA.ORG>
Message-ID: <77DD817201982748BC67D7960F2F76AF0DBDDD@UWHC-MBX12.uwhis.hosp.wisc.edu>

Sorry, I thought we were talking IHC/ISH not SS.

Linda A. Sebree
________________________________________
From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cooper, Brian [bcooper@chla.usc.edu]
Sent: Wednesday, September 17, 2014 2:36 PM
To: Morken, Timothy; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Automated Special Stainer...

I guess "TAT's being equal" is a relative term, depending upon your workload.  For our purposes, it's not slide capacity that poses a problem--it's reagent capacity.  We've never loaded 50 special stains at any one time--we typically don't even have half that amount in one day!

So here's a scenario common to the workload at our institution.  For our liver panels, we run Iron, Trichrome, Retic, PAS with and without Digestion.  That pretty much maxes out the reagent capacity on the Benchmark.  Sure, we can load up to 20 of these onto the Benchmark.  But as is so frequently the case, we also have just 1 GMS that also needs to be stained!  This is where the second Benchmark comes into play, or we'll have to stain by hand.   One Artisan can handle this at the same time, and if memory serves (disclosure--it's been about 2 years, and the model I used didn't have online deparaffinization), it didn't add all that much time to the process.  It was certainly faster than waiting for the machine to complete, and start another run.   For our institution, it's either going to be 2 Artisans or 2 Benchmarks.  The Artisans will give us some greater flexibility due to the greater reagent capacity.

Brian


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, September 17, 2014 10:58 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Automated Special Stainer...

Brian wrote: "... one more thing.  The reagent tray capacity on the Artisan is about twice what it is on a Benchmark."

Are the TAT's equal? When we loaded up our old artisan with slides (50) and full of reagents it took over 5 hours to finish - way past our deadline. So nobody wanted to use it that way so the techs resisted and wanted to do manual to get the slide out. We ended up putting only a few stains on it.

We  are looking at doing specials the way we do immunos - on demand all day rather than in one batch in the AM.

It seemed to me that the 20-slide units from Ventana allow small batches and run in parallel or allow staggered use. Buying a 50-slide capacity instrument and then putting only 20 slides at a time on it seems odd somehow.


We looking at each of these as well and not sure yet which would serve us better. We do about 60-80 specials slides per day, and 13 stains give us 90% of our volume.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Wednesday, September 17, 2014 10:33 AM
To: Sarah.Dysart@stdavids.com; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Automated Special Stainer...

Dako Artisan all the way!!!!!   I've used both systems (the Artisan in my old institution).  We currently have a few of the new Benchmarks on demo right now.  Thus far, we're not that impressed.  Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan.  Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab).   Oh yeah, one more thing.  The reagent tray capacity on the Artisan is about twice what it is on a Benchmark.  You'll need two Benchmarks to do what one Artisan does, TATs being equal.

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper@chla.usc.edu


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah.Dysart@stdavids.com
Sent: Wednesday, September 17, 2014 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Automated Special Stainer...

Opinions on Ventana versus Dako...Go!

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From mw <@t> personifysearch.com  Wed Sep 17 15:07:10 2014
From: mw <@t> personifysearch.com (Matt Ward)
Date: Wed Sep 17 15:07:17 2014
Subject: [Histonet] Field Based Histology Opportunity - Northeast
Message-ID: <0a6a01cfd2b2$f7b416a0$e71c43e0$@personifysearch.com>

Good afternoon,


We have had a world leading histology manufacturer open a new Field Based
Histology opportunity in the Northeast. The Field Specialist will cover
upstate NY and New England and the position is open due to promotion. The
ideal candidate will have a strong background in Histology and a 4 year
degree. 


Please e-mail me directly at mw@personifysearch.com
<mailto:mw@personifysearch.com>  to learn more. 

 

Thanks!


Matt 

 

Matt Ward

Program Manager 

Personify

5020 Weston Parkway Suite 315

Cary NC 27513 

(Tel) 919.459.3654

(Tel) 800.875.6188 direct ext 103

(Fax) 919.882.8727

  <http://www.personifysearch.com/> www.personifysearch.com

 

From cjohnson <@t> nmda.nmsu.edu  Wed Sep 17 15:12:41 2014
From: cjohnson <@t> nmda.nmsu.edu (Johnson, Carole)
Date: Wed Sep 17 15:12:46 2014
Subject: [Histonet] rabbit to rabbit IHC
Message-ID: <F00D04066B62F04480A441B7460C17B5C642BDF7@EX-MBX-P1.ACN.ad.nmsu.edu>

Is anyone using the rabbit to rabbit blocking reagent from ScyTek? I am trying to run tularemia antiserum on rabbit tissue. Thanks in advance!

Carole Johnson, HT(ASCP)cm
Histopathology Section Supervisor
New Mexico Department of Agriculture
Veterinary Diagnostic Services




Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act.
From TNMayer <@t> mdanderson.org  Wed Sep 17 15:39:11 2014
From: TNMayer <@t> mdanderson.org (Mayer,Toysha N)
Date: Wed Sep 17 15:39:15 2014
Subject: [Histonet] RE:Automated Special Stainer...
Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881B48BBAE@D1PWPEXMBX05.mdanderson.edu>

Sarah,

The costs of Ventana are high.  If you have a steady workload, and/or  inexperienced techs it is ok.  If the stainer malfunctions, you have no way of knowing where it stopped to continue by hand.  If your reagents are 1 day expired, you cannot change the computer date and continue.  (I know I'm not the only person who has done this).
Dako is easier to use, easier to fool, and gives more consistent results.  The dates can be changed to continue usage, the system allows you to develop your own protocols if you use a different counterstain, and you will always know where the unit stops to manually continue if needed.  

Hope this helps.
Sincerely,

Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
UT M.D. Anderson Cancer Center
713.563-3481


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Message: 16
Date: Wed, 17 Sep 2014 11:13:14 -0500
From: <Sarah.Dysart@stdavids.com>
Subject: [Histonet] Automated Special Stainer...
To: <histonet@lists.utsouthwestern.edu>
Message-ID:
	<34C22BB94729434598D767D3F4EB95E0E2174B0D69@FWDCWPMSGCMS03.hca.corpad.net>
	
Content-Type: text/plain; charset="us-ascii"

Opinions on Ventana versus Dako...Go!

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220



------------------------------

Message: 17
Date: Wed, 17 Sep 2014 16:30:18 +0000 (UTC)
From: Sue <suetp918@comcast.net>
Subject: Re: [Histonet] Automated Special Stainer...
To: Sarah Dysart <Sarah.Dysart@stdavids.com>
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <81116639.3933059.1410971418142.JavaMail.root@comcast.net>
Content-Type: text/plain; charset=utf-8

Have Ventana like it but cost is high, ave no experience with new DAKO but old platform was nice ?? 

Sue Paturzo 

From delsuec <@t> gmail.com  Wed Sep 17 16:47:58 2014
From: delsuec <@t> gmail.com (Deloris Carter)
Date: Wed Sep 17 16:48:04 2014
Subject: [Histonet] From: Deloris Carter
Message-ID: <20140917214759.53D6C359AB13@mail.ewhost.com>

Hi histonet


http://gardaradio.com/stand.php?pass=chnxgegn3135nybqx



delsuec@gmail.com


From kmerriam2003 <@t> yahoo.com  Thu Sep 18 10:47:56 2014
From: kmerriam2003 <@t> yahoo.com (Kim Merriam)
Date: Thu Sep 18 10:50:46 2014
Subject: [Histonet] anti-mouse NK marker
Message-ID: <1411055276.86790.YahooMailBasic@web121904.mail.ne1.yahoo.com>

Hi,

Does anyone have a good anti-mouse NK cell marker for frozen mouse tissues?

Thanks,
Kim



Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA

From TanyaAbbott <@t> catholichealth.net  Thu Sep 18 11:13:10 2014
From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya)
Date: Thu Sep 18 11:19:12 2014
Subject: [Histonet] More info on EBUS
Message-ID: <852F7D2C14FB464D80E182B15DB138AF394D3869@CHIEX005.CHI.catholichealth.net>

Sorry I was a little vague on my quest for answers!! It was our first one,............!
Pathologist was down in the Lab (we are a small hospital) and slides were sent down. He gave a dx/adequacy (none of them were really adequate for a proper dx) for each slide, they sampled 3 levels multiple times, some passes were grouped together. Slides came down about 7 different times for a total of 22. We received slides that some appeared to be "dropped on" and some were smears. And then after the procedure received aspirates in Cytolyte.
It was a very interesting day!
Much thanks!  Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabbott@catholichealth.net




This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.
From twebster <@t> CRH.org  Thu Sep 18 12:33:05 2014
From: twebster <@t> CRH.org (Webster, Thomas S.)
Date: Thu Sep 18 12:33:15 2014
Subject: [Histonet] RE: Histonet Digest, Vol 130, Issue 18
In-Reply-To: <201409181706.s8IH6BFT022731@l31.spamh.com>
References: <201409181706.s8IH6BFT022731@l31.spamh.com>
Message-ID: <7207186ED68FB542803CAF1CE6E82FF80871B164@exmb1.crh.org>

Sounds like it probably was all needle aspirations if they put some passes in cytolyt.  If I am understanding your procedure, it appears there were 7 billable evaluation events. Bill 88172 for the first one and 88177x6 for the others. 

You probably already know to bill 88173 for the final interpretation and 88305 if you made a cell block on the cytolyt specimen. 



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Thursday, September 18, 2014 1:06 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 130, Issue 18

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Today's Topics:

   1. More info on EBUS (Abbott, Tanya)


----------------------------------------------------------------------

Message: 1
Date: Thu, 18 Sep 2014 16:13:10 +0000
From: "Abbott, Tanya" <TanyaAbbott@catholichealth.net>
Subject: [Histonet] More info on EBUS
To: "histonet@lists.utsouthwestern.edu"
	<histonet@lists.utsouthwestern.edu>
Message-ID:
	<852F7D2C14FB464D80E182B15DB138AF394D3869@CHIEX005.CHI.catholichealth.net>
	
Content-Type: text/plain; charset="us-ascii"

Sorry I was a little vague on my quest for answers!! It was our first one,............!
Pathologist was down in the Lab (we are a small hospital) and slides were sent down. He gave a dx/adequacy (none of them were really adequate for a proper dx) for each slide, they sampled 3 levels multiple times, some passes were grouped together. Slides came down about 7 different times for a total of 22. We received slides that some appeared to be "dropped on" and some were smears. And then after the procedure received aspirates in Cytolyte.
It was a very interesting day!
Much thanks!  Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph  610-378-2635 fax 610-898-5871
email: tanyaabbott@catholichealth.net




This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.


------------------------------

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 130, Issue 18
*****************************************


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From abtdhu <@t> gmail.com  Thu Sep 18 14:08:44 2014
From: abtdhu <@t> gmail.com (Dorothy Hu)
Date: Thu Sep 18 14:08:48 2014
Subject: [Histonet] Re: Histonet Digest, Vol 130, Issue 16
In-Reply-To: <5419be3b.8180b60a.46c6.fffffddcSMTPIN_ADDED_MISSING@mx.google.com>
References: <5419be3b.8180b60a.46c6.fffffddcSMTPIN_ADDED_MISSING@mx.google.com>
Message-ID: <CAJ2-qz408z=jisT4yEmOJgKEHYwN2kAJoLHP4TQ7iNNE9MTEoA@mail.gmail.com>

Hi Dear Histoneters,
Can you tell me which kind of Brady labeler you are using? I want to get
one. Do you have catalog number? Many thanks.
Dorothy
MGH
Endocrine histocore

>
>
> I use the Brady system and I like it a lot.  I work in a small animal
> diagnostic lab and will have 60-80 blocks on most days.  It saves me time
> and even better I don't have to read my writing in the morning.  I use it
> for the cassettes and slides mainly but I label reagents I mix with it to.
> I find it very versatile.
> Roberta Horner HT/HTL
> Animal Diagnostic Lab
> Penn State University
>
>
> Subject: [Histonet] Slide and cassette printers?
>
> What are all of you using these days?  What would be good for a small
> histo lab that has the potential to grow?  (I am already aware of what
> Leica and Thermo have from the archives here, but I am still interested in
> your opinions, of course.)  Has anyone tried the printers, labels and
> attachment machine from Brady?
>
> Thank you all so much,
> Kathleen
>
>
> Principal Lab Technician
> Histopathology Lab
> Office of Translational Sciences
> Rutgers, the State University of NJ
> 41 B Gordon Road
> Piscataway, NJ 08854
> (848) 445-1443
> FAX (732) 445-6905
>
>
>
From hfedor <@t> jhmi.edu  Thu Sep 18 14:13:44 2014
From: hfedor <@t> jhmi.edu (Helen Fedor)
Date: Thu Sep 18 14:13:49 2014
Subject: [Histonet] Re: Histonet Digest, Vol 130, Issue 16
In-Reply-To: <CAJ2-qz408z=jisT4yEmOJgKEHYwN2kAJoLHP4TQ7iNNE9MTEoA@mail.gmail.com>
References: <5419be3b.8180b60a.46c6.fffffddcSMTPIN_ADDED_MISSING@mx.google.com>
	<CAJ2-qz408z=jisT4yEmOJgKEHYwN2kAJoLHP4TQ7iNNE9MTEoA@mail.gmail.com>
Message-ID: <BCE07FB3A86FD040969A9EC0DD3EDA0D1747C08B@JHEMTMWEX4.win.ad.jhu.edu>

We had the PTL, which is a little more cumbersome. We are now using the BBP33, and are very happy with it.


Helen L. Fedor 

Prostate Tissue Bank, Manager
Oncology Tissue Services, Manager
Johns Hopkins University
600 N. Wolfe St,?| Marburg Room 406
Baltimore, MD?| 21287-7065

410-614-1660 (Marburg)
443-287-7338 (Bond St.)

http://tmalab.jhmi.edu/
http://prostatebiorepository.org/





-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dorothy Hu
Sent: Thursday, September 18, 2014 3:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet Digest, Vol 130, Issue 16

Hi Dear Histoneters,
Can you tell me which kind of Brady labeler you are using? I want to get one. Do you have catalog number? Many thanks.
Dorothy
MGH
Endocrine histocore

>
>
> I use the Brady system and I like it a lot.  I work in a small animal 
> diagnostic lab and will have 60-80 blocks on most days.  It saves me 
> time and even better I don't have to read my writing in the morning.  
> I use it for the cassettes and slides mainly but I label reagents I mix with it to.
> I find it very versatile.
> Roberta Horner HT/HTL
> Animal Diagnostic Lab
> Penn State University
>
>
> Subject: [Histonet] Slide and cassette printers?
>
> What are all of you using these days?  What would be good for a small 
> histo lab that has the potential to grow?  (I am already aware of what 
> Leica and Thermo have from the archives here, but I am still 
> interested in your opinions, of course.)  Has anyone tried the 
> printers, labels and attachment machine from Brady?
>
> Thank you all so much,
> Kathleen
>
>
> Principal Lab Technician
> Histopathology Lab
> Office of Translational Sciences
> Rutgers, the State University of NJ
> 41 B Gordon Road
> Piscataway, NJ 08854
> (848) 445-1443
> FAX (732) 445-6905
>
>
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From raj <@t> bluemarble.net  Thu Sep 18 14:25:53 2014
From: raj <@t> bluemarble.net (Rebecca a. Johnson)
Date: Thu Sep 18 14:26:12 2014
Subject: [Histonet] New email address
Message-ID: <49B129C915734C7FB7D55C4401B531FD@BeckyPC>

            

            Please add my new email address; histotech1948@gmail.com
From HornHV <@t> archildrens.org  Thu Sep 18 14:53:01 2014
From: HornHV <@t> archildrens.org (Horn, Hazel V)
Date: Thu Sep 18 14:53:09 2014
Subject: [Histonet] job opening in little rock, ar
Message-ID: <25A4DE08332B19499904459F00AAACB719E29EFEFC@EVS1.archildrens.org>

Arkansas Children's Hospital, located in Little Rock, AR has a job opening in our histology lab.  We are a small lab serving the children of Arkansas. We perform special stains by hand, Immunos on a Bond and routine histology.  The position is the "late shift" position from 8am until 4:30pm, Monday-Friday.  We rotate call for major holidays.  Arkansas Children's Hospital has competitive salaries and good benefits.
Please apply online.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hornhv@archildrens.org<mailto:hornhv@archildrens.org>
archildrens.org<http://www.archildrens.org/>







******************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************
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prohibited. If you have received this communication in error, please notify 
us immediately by replying to the message and deleting it from your computer.
Thank you.
From dmaney22 <@t> yahoo.com  Fri Sep 19 07:14:45 2014
From: dmaney22 <@t> yahoo.com (Donna Maney)
Date: Fri Sep 19 07:14:50 2014
Subject: [Histonet] Artifact in IHC
Message-ID: <1411128885.65969.YahooMailBasic@web125401.mail.ne1.yahoo.com>

Hello,
I've been doing IHC on free-floating brain sections for about 15 years using the same protocol: primary raised in rabbit, Vector goat anti-rabbit secondary, Vector ABC Elite kit, then DAB (either nickel-enhanced or not). On the most recent run of tyrosine hydroxylase-labeled tissue we got a lot of weird looking artifact. I am going to upload an image showing the artifact at several magnifications. 

- When looking at the slide with the naked eye, the artifact looks like a white crust. Under the scope, it looks black.
- It appears in patches on the tissue, not over the rest of the slide. 
- It appears in nickel-enhanced (purple, shown in photo) AND regular (brown) DAB stained tissue. 
- It appears even if primary and/or secondary antibody are omitted.
- It does not always appear in the same area of the section. Some sections do not have it at all.
- All of the tissue was from the same brain.

Brains were immersion-fixed in 5% acrolein, sunk in 30% sucrose and stored at -80? for about a year. They were then cut at 50?m on a freezing sliding microtome and stored in cryoprotectant at -20 for about a week before the IHC. These storage times have not previously been a problem for this IHC.

The IHC was run using 0.01M PBS, pH 7.4. 

The stained sections were mounted on gelatin-subbed slides and left to dry overnight. Then they were dehydrated in increasing concentrations of ethanol, then xylenes, then coverslipped in DPX.

Here is the image: http://histosearch.com/imageupload/artifact-in-ihc/

Thanks for any ideas about what this could be.
Donna


From KSimeone <@t> leavittmgt.com  Fri Sep 19 12:07:33 2014
From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone)
Date: Fri Sep 19 12:07:38 2014
Subject: [Histonet] FT NIGHT SHIFT Histotech position in DELRAY BEACH, FL
Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C2FDA351D@vm-email.leavittmgt.com>

Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE.



***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!



Email your resume to lengimann@leavittmgt.com<mailto:lengimann@leavittmgt.com> if interested.



*full time position Mon-Fri PM (will discuss hours upon interview/will be AFTER 5p)

*MUST be licensed as a FL histotechnologist or technician

*MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE!

*VERY proficient in embedding and microtomy

*must be self motivated, reliable and a team player

*knowledge in operating Ventana and Leica equipment desired (not necessary)



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

ksimeone@leavittmgt.com<mailto:ksimeone@leavittmgt.com>





The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message.


From christina.kreutzer01 <@t> gmail.com  Sun Sep 21 02:25:16 2014
From: christina.kreutzer01 <@t> gmail.com (Christina Kreutzer)
Date: Sun Sep 21 02:25:52 2014
Subject: [Histonet] Whole rat head histology
In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32012526AA1F@Mail2Node2.ad.uams.edu>
References: <CAOKsRH4fXNp+HfG-LSoBVbiQbNaP_V0tpzvG5vUHascceiPB6g@mail.gmail.com>
	<0B8979A204680A42B93A52B486088CD93938636C72@CUAEXH1.GCU-MD.local>
	<389C7D597C19C94D9E6A51F6CAC225F35282DB5B@skimmer.medcampus.org>
	<8D1A0A47F163FC8-1464-170E@webmail-vm090.sysops.aol.com>
	<41D3A1AF6FEF0643BDC89E0516A6EA32012526AA1F@Mail2Node2.ad.uams.edu>
Message-ID: <CANS3E9tkeBWrJt==Jckdt03a9WTN+JC2cSmeTEbqjq35rAK47w@mail.gmail.com>

I want to do the same, with adult rats. doing paraffin sections seems to be
the best option I guess
Am 17.09.2014 18:02 schrieb "Marcum, Pamela A" <PAMarcum@uams.edu>:
>
> How old are the rats?
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:
histonet-bounces@lists.utsouthwestern.edu] On Behalf Of William J. O'Connor
III
> Sent: Wednesday, September 17, 2014 10:38 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Whole rat head histology
>
>
> I have a fun project - processing a whole rat head, skull with brain, for
serial sections to see a brain/skull congenital deformity.    Anyone have
any experience with this?  I'd love it if you could share your protocol.
Paraffin or plastic?
> Jackie O'
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ----------------------------------------------------------------------
> Confidentiality Notice: This e-mail message, including any attachments,
is for the sole use of the intended recipient(s) and may contain
confidential and privileged information. Any unauthorized review, use,
disclosure or distribution is prohibited. If you are not the intended
recipient, please contact the sender by reply e-mail and destroy all copies
of the original message.
>
> _______________________________________________
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From mitchell <@t> wanpost.com  Mon Sep 22 05:24:39 2014
From: mitchell <@t> wanpost.com (Mitchell Wan)
Date: Mon Sep 22 05:24:53 2014
Subject: [Histonet] Need help with equipment for a New Laboratory
Message-ID: <00c501cfd64f$6aedbf00$40c93d00$@com>

Hi All,

I am looking to purchase equipment to set up a new AP laboratory.

 

Looking at second hand quality Leica :

Tissue Processors

Coverslippers

Stainer - preferably ST4040

 

Any brand:

Embedding Centres

Low profile Manual microtomes

Water Baths

Cold Plates


Anything to do with Histology - I am interested.

 

I can pick up in Asia or organise shipment.

 

Needs to be 220-240V

 

I am looking for members to assist as required.

 

Regards
Mitchell Wan

P.O.Box 2200,
Runcorn,
Brisbane,
QLD 4113.

0418 745 750
mitchell@wanpost.com



 



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From mcauliff <@t> rwjms.rutgers.edu  Mon Sep 22 08:39:52 2014
From: mcauliff <@t> rwjms.rutgers.edu (Geoff)
Date: Mon Sep 22 08:39:54 2014
Subject: [Histonet] Artifact in IHC
In-Reply-To: <1411128885.65969.YahooMailBasic@web125401.mail.ne1.yahoo.com>
References: <1411128885.65969.YahooMailBasic@web125401.mail.ne1.yahoo.com>
Message-ID: <542026A8.2010109@umdnj.edu>

Assuming that the 'crust' is a precipitate, what is it soluble in? 
Water, alcohol, dilute acid or base?
It shows up if you just run the peroxide+DAB rxn?

Geoff

On 9/19/2014 8:14 AM, Donna Maney wrote:
> Hello,
> I've been doing IHC on free-floating brain sections for about 15 years using the same protocol: primary raised in rabbit, Vector goat anti-rabbit secondary, Vector ABC Elite kit, then DAB (either nickel-enhanced or not). On the most recent run of tyrosine hydroxylase-labeled tissue we got a lot of weird looking artifact. I am going to upload an image showing the artifact at several magnifications.
>
> - When looking at the slide with the naked eye, the artifact looks like a white crust. Under the scope, it looks black.
> - It appears in patches on the tissue, not over the rest of the slide.
> - It appears in nickel-enhanced (purple, shown in photo) AND regular (brown) DAB stained tissue.
> - It appears even if primary and/or secondary antibody are omitted.
> - It does not always appear in the same area of the section. Some sections do not have it at all.
> - All of the tissue was from the same brain.
>
> Brains were immersion-fixed in 5% acrolein, sunk in 30% sucrose and stored at -80? for about a year. They were then cut at 50?m on a freezing sliding microtome and stored in cryoprotectant at -20 for about a week before the IHC. These storage times have not previously been a problem for this IHC.
>
> The IHC was run using 0.01M PBS, pH 7.4.
>
> The stained sections were mounted on gelatin-subbed slides and left to dry overnight. Then they were dehydrated in increasing concentrations of ethanol, then xylenes, then coverslipped in DPX.
>
> Here is the image: http://histosearch.com/imageupload/artifact-in-ihc/
>
> Thanks for any ideas about what this could be.
> Donna
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732) 235-4583; fax: -4029
mcauliff@rwjms.rutgers.edu
**********************************************



From kstoll <@t> mcw.edu  Mon Sep 22 11:09:36 2014
From: kstoll <@t> mcw.edu (Stoll, Kathryn)
Date: Mon Sep 22 11:09:40 2014
Subject: [Histonet] Dako CMV
Message-ID: <CC20126F2AD4A142AFF0700D5903DAFE3C81F64F@MCWMB5.mcwcorp.net>

Hi,  I need to work up the Dako CMV for a project.  I have an Autostainer Plus and an Autostainer Link 48.  Would someone be willing to share their procedure with me?
Many Thanks,

Kathryn Stoll
Supervisor Histology
Clinical and Translational Research Core Lab
Medical College of Wisconsin
9200 W. Wisconsin Ave Room 1176
Milwaukee WI 53226
Phone: 414-805-1525
Fax: 414-805-1528

From tejohnson <@t> genoptix.com  Mon Sep 22 12:17:32 2014
From: tejohnson <@t> genoptix.com (Teri Johnson)
Date: Mon Sep 22 12:17:58 2014
Subject: [Histonet] RE: Artifact in IHC
Message-ID: <39b2c52d849d446588309f03536993e7@PHUSCB-SP37MB03.genoptix.org>

Hi Donna,

Interesting conundrum! A few questions:
 - Do you see it in tissues that have not undergone any staining, just floated in buffer and coverslipped?
 - Have you tried wet mounting them after staining and prior to dehydration and clearing to see if the artifact is there?
 - Have you recently changed glove manufacturers, could this be talc or some other powder from gloves?
 - Are you dehydrating out of PBS into an alcohol concentration higher than 70%? You could be getting phosphate precipitation. Try using a water rinse prior to alcohol or make sure your first alcohol concentration is 70% or lower.

Those are the weird things that came to mind. I hope this helps,

Teri Johnson
Manager Clinical Trial Testing
Genoptix, Inc.
Carlsbad, CA 92008



________________________________

CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message.


From cjbulmer <@t> sbcglobal.net  Mon Sep 22 12:28:36 2014
From: cjbulmer <@t> sbcglobal.net (Cindy Bulmer)
Date: Mon Sep 22 12:28:42 2014
Subject: [Histonet] Dako CMV
In-Reply-To: <CC20126F2AD4A142AFF0700D5903DAFE3C81F64F@MCWMB5.mcwcorp.net>
References: <CC20126F2AD4A142AFF0700D5903DAFE3C81F64F@MCWMB5.mcwcorp.net>
Message-ID: <1411406916.22931.YahooMailNeo@web180906.mail.ne1.yahoo.com>

I use the Dako CMV concentrated Ab with a 1:6000 titer using the Mouse LP and AR 6.0
Hope this help, good luck!

Cindy

Cynthia Bulmer HT(ASCP)QIHC 
IHC Supervisor, CTPL 
Waco, TX
 

________________________________
 From: "Stoll, Kathryn" <kstoll@mcw.edu>
To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> 
Sent: Monday, September 22, 2014 11:09 AM
Subject: [Histonet] Dako CMV
  

Hi,  I need to work up the Dako CMV for a project.  I have an Autostainer Plus and an Autostainer Link 48.  Would someone be willing to share their procedure with me?
Many Thanks,

Kathryn Stoll
Supervisor Histology
Clinical and Translational Research Core Lab
Medical College of Wisconsin
9200 W. Wisconsin Ave Room 1176
Milwaukee WI 53226
Phone: 414-805-1525
Fax: 414-805-1528

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From gayle.callis <@t> bresnan.net  Mon Sep 22 13:26:34 2014
From: gayle.callis <@t> bresnan.net (gayle callis)
Date: Mon Sep 22 13:26:57 2014
Subject: [Histonet] Re:  Processing whole rat heads
Message-ID: <001201cfd692$bf362640$3da272c0$@bresnan.net>

You will need to totally fix the heads over a longer period of time.
Perfusion is ideal if you can to that, followed by immersion fixation for a
week.   Otherwise, simply remove the head and immerse into a very large
volume of NBF.    If you only want to see is the skull and brain, you can
disarticulate and remove the lower jaw carefully so as to not damage the
skull.   We removed the skin and ears by stripping the skin from back of
head towards the nose  leaving the flesh around the end of nose(nares)
intact, before immersing in NBF.    The eyes can be left in and are good
landmarks during microscopy.  

 

Immersion fixation should be done for 7 to 10 days, longer being better.  I
suggest changing the NBF after the first or second day, and then again after
several days to replenish the aldehyde component.  You want total fixation
in order to protect the soft tissues and nuclear staining from effects of
acid decalcification.    

 

Decalcification can be done with 15 to 20% formic acid.  If you are not
planning to do any immunohistochemistry or special stains affected by the
acid decalcifying agents, a commercial HCl based decalcification will speed
up decalcification.  Change the decalcifying solution daily, and do
decalcification endpoint testing to prevent over exposure to either formic
and HCl acids i.e. "over decalcification' that damages nuclear staining,
especially H&E.   The main thing is to fix and decalcify the teeth and inner
ear bones completely as these will take longer to decalcify than the thinner
skull bones.    I suggest suspending the heads during fixation and
decalcification so these solutions surround the head completely.  Large
nylon biopsy bags work nicely, can be suspended with strings into solutions.
Although we did not attempt to remove teeth and if you don't need to see
them, this could be done after decalcification  and the water rinse.  Cut
off incisors  with a very sharp cuticle scissors (yes, the ones found in
nail salons and very cheap at Walmart).  Be careful to not disrupt the bone
when doing this.  The molar and other teeth will probably have to remain
since pulling those teeth will disrupt bone/soft tissues.    

 

After decalcification is complete, and if waiting for other heads to finish
decalcifying, rinse the heads in running tap water for 4 hours and them
transfer to either NBF to stop the decalcification.    One can transfer to
70% alcohol which is then the first solvent used in the processing schedule.
Do not go back into NBF after storing in 70% alcohol.        

 

Your processing needs to be extended considerably with the whole rat head.
Automated processing should be done with  vacuum and pressure.  You may end
up having to process for 2 1/2 to 3 hours per station with ambient
temperature (no heat added) and vacuum on, depending on the processor.  I
suggest trial runs with normal rat heads to optimize processing.  This will
require a dedicated schedule in order to have good dehydration, clearing and
paraffin infiltration with a minimum of three paraffin changes, no less.
Harder paraffin is desirable but not necessary i.e. Tissue Prep 2 or an
equivalent,  to support the head during sectioning.   Heat added to
processing only dries out rodent tissues/bones excessively.    We processed
heads in large nylon bags but hold the bags down securely so they don't
float while in the processor chamber.   We used a  curved hemostat to clamp
tops of bags and hold them down inside the metal processor baskets.  There
will be a certain amount of shrinkage of soft brain from bone due to
processing solvents.   

 

We embedded in the largest Peel a Way mold (I have photos of how to do this
if you need them, and will send privately).   This way you avoid those
annoying, unstable,  large mega cassettes the move around in the block
holder, and probably too small for a rat head anyway.   Before embedding,
put labeled cassette backs and largest Peel away molds in embedding center
to be hot during embedding procedure.   Fill hot mold with melted paraffin,
embed head and anchor head on cold plate to prevent floating up/maintain
orientation.   The head orientation is for mid sagittal cuts.  Working
quickly, push the hot, labeled  tissue cassette(with lid removed)  down to
but not quite touching the side of the head.   It takes practice but the hot
cassette can be pushed down evenly.  We use two thumb dressing forceps to do
this, and could level the cassette fairly easily simply by eye.  Just get
down to eye level to see this while the paraffin is hot.   The hot peel away
mold expands outwards without tearing apart when adding the cassette back.
Be gentle!     This embedment results in a block that doesn't extend
excessively from block holder  and avoids unnecessary/unstable mechanical
forces during sectioning.  The regular cassette back (in horizontal
position) fits snugly in a block holder for stable sectioning.  Trim away
excess paraffin from back, sides and ends of cassette to fit in holder
evenly.    If you try to orient the block so it is on vertical axis rather
than horizontal,  your sectioning will be more difficult., fewer sections in
ribbon for flotation.    The top of skull is rounded so that should take
care of trying to embed the skull at an angle, or you can try more of an
angle if it works for you.    The old fashioned way is to lay each ribbon on
a flat black construction paper, cut the section from ribbon, float and pick
up each section so as to NOT lose a serial section.  This way you can have
many ribbons laying out in sequence before floatation.  Tedious but
accurate.    

 

A regular rotary style  paraffin microtome should work without problems.  5
to 7 um should be adequate.   Overly thick sections will curl up, making
them hard to flatten on water bath.  To section, you should mount the block
in holder so the edge of the blade passes through top of the skull first and
those tough, harder teeth last.  Teeth and inner ear bones will be the
hardest thing to section.   I strongly suggest using high profile disposable
blades for stability as these are just as sharp as low profile.   Doing
serial sections will not be a simple task but certainly possible, requires
practice and patience.   Mount sections on plus charge slides, drain water
off well and dry sections FLAT at 37?C to 40?C for several days.  Do NOT dry
in a hot oven.  Bones, teeth and brain should flatten and adhere well
without excessive drying heat.  Make sure the blade is always sharp, changed
frequently, and soaking not excessive.  You may want to soak the block face
with a gauze wet with cold water with icing the wet gauze frequently while
the block mounted remains in the block holder so you can pick up all
sections.  After a each soak, you will have to back up the block a tiny bit
in order to get the next section in the series.    

 

For staining, avoid overly vigorous rinsing as the softer brain can still
dislodge from bone and/or fold over.    

 

Good luck and send photos to histonet when you have results.      

 

Gayle Callis

HTL/HT/MT(ASCP)   

   

From mcauliff <@t> rwjms.rutgers.edu  Mon Sep 22 13:40:53 2014
From: mcauliff <@t> rwjms.rutgers.edu (Geoff)
Date: Mon Sep 22 13:41:00 2014
Subject: [Histonet] RE: Artifact in IHC
In-Reply-To: <39b2c52d849d446588309f03536993e7@PHUSCB-SP37MB03.genoptix.org>
References: <39b2c52d849d446588309f03536993e7@PHUSCB-SP37MB03.genoptix.org>
Message-ID: <54206D35.6030504@umdnj.edu>

Teri makes an excellent point about higher alcohols causing 
precipitation of phosphate salts.

Geoff

On 9/22/2014 1:17 PM, Teri Johnson wrote:
> Hi Donna,
>
> Interesting conundrum! A few questions:
>   - Do you see it in tissues that have not undergone any staining, just floated in buffer and coverslipped?
>   - Have you tried wet mounting them after staining and prior to dehydration and clearing to see if the artifact is there?
>   - Have you recently changed glove manufacturers, could this be talc or some other powder from gloves?
>   - Are you dehydrating out of PBS into an alcohol concentration higher than 70%? You could be getting phosphate precipitation. Try using a water rinse prior to alcohol or make sure your first alcohol concentration is 70% or lower.
>
> Those are the weird things that came to mind. I hope this helps,
>
> Teri Johnson
> Manager Clinical Trial Testing
> Genoptix, Inc.
> Carlsbad, CA 92008
>
>
>
> ________________________________
>
> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732) 235-4583; fax: -4029
mcauliff@rwjms.rutgers.edu
**********************************************



From melissa <@t> alliedsearchpartners.com  Tue Sep 23 09:47:39 2014
From: melissa <@t> alliedsearchpartners.com (Melissa Owens (Phelan))
Date: Tue Sep 23 09:47:54 2014
Subject: [Histonet] Direct Hire/Permanent Histotech Job in Minnesota
Message-ID: <D047004B.5BC50%melissa@alliedsearchpartners.com>

Good Morning,

I have a permanent/direct hire position available for a Histotech in
Rochester, MN area. Please contact me for the details if you are interested.
Thank you!

Melissa Owens (Phelan)
President, Laboratory Staffing
Allied Search Partners
www.linkedin.com/in/melissaphelan/
<http://www.linkedin.com/in/melissaphelan/>
http://www.alliedsearchpartners.com <http://www.alliedsearchpartners.com/>

T: 888.388.7571 ext. 102

F: 888.388.7572






From lpjones <@t> srhs-pa.org  Tue Sep 23 09:49:03 2014
From: lpjones <@t> srhs-pa.org (Jones, Laura)
Date: Tue Sep 23 09:49:07 2014
Subject: [Histonet] JCAHO Competency Requirements
Message-ID: <EA9F46DFE9B0DB4DB40F0CA9842277BB0F9AEA5149@EXCH2007.srhs-pa.org>

Good Morning!  We are switching from CAP to JCAHO for our inspection and accreditation.  I'm reading a lot about "Six Method Competency", but most of the information I have is geared toward blood testing.  If anyone out there can offer any suggestions as to how I should reformat our competency forms to satisfy JCAHO, I would appreciate any help.  Thanks in advance!


Laura Jones B.A., HT, PBT (ASCP) | Lead Tech, Histology | Community Health Systems
740 East State Street | Sharon PA | Phone (724)983-3950 | Fax (724)983-3982
www.sharonregional.com<http://www.sharonregional.com/> | lpjones@srhs-pa.org<mailto:lpjones@srhs-pa.org>


________________________________
Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains.
From gayle.callis <@t> bresnan.net  Tue Sep 23 11:09:11 2014
From: gayle.callis <@t> bresnan.net (gayle callis)
Date: Tue Sep 23 11:09:34 2014
Subject: [Histonet] At long last,
	a Murine CD4 monoclonal antibody that works on FFPE tissues
Message-ID: <003201cfd748$b895b5b0$29c12110$@bresnan.net>

Dear Histonetters, 

 

This antibody is going to be very welcome for those working with NBF fixed
paraffin embedded murine tissue.    It is a rat antiMouse CD4  for FFPE
tissue.   For those interested go to Affymetrix/eBioscience, Rat antiMouse
CD4, clone 4SM95 (L3T4/Ly-4,  Catalog number 14-9766.   It also comes
conjugated to the company's eFluor 570, equivalent to Alexa 555 although one
could do their own conjugation to their favorite Alexa or DyLight dye.   

 

Now all we need is a mouse CD8 that works on FFPE for total happiness since
we often do these two antibodies in tandem as a double IF stain or on
adjacent sections for enzyme IHC.    

 

Take care

 

Gayle M. Callis

HTL/HT/MT(ASCP)  

From Debbie.Lake <@t> mgh.net  Tue Sep 23 12:22:35 2014
From: Debbie.Lake <@t> mgh.net (Lake,Debbie)
Date: Tue Sep 23 12:22:47 2014
Subject: [Histonet] Surgical Pathology reports
Message-ID: <96C654E4B0F52748948EFA2938B5DEE2ED3527B3@mghemail02.mgh.net>

Do pathologists ever call pathology reports to a physician?  If so, what types of diagnoses do they call?  There are no "critical" test results in Pathology that CAP deems necessary to call as in other areas of the laboratory..  How do others handle this?

Debra Lake  MT(ASCP)
Manager Micro, Blood Bank, Pathology
Marion General Hospital
Marion, IN  46952
(765) 660-6521
Fax: (765-651-7330)






If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately.
From Toni.Rathborne <@t> rwjuh.edu  Tue Sep 23 12:34:16 2014
From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni)
Date: Tue Sep 23 12:34:24 2014
Subject: [Histonet] RE: Surgical Pathology reports
In-Reply-To: <96C654E4B0F52748948EFA2938B5DEE2ED3527B3@mghemail02.mgh.net>
References: <96C654E4B0F52748948EFA2938B5DEE2ED3527B3@mghemail02.mgh.net>
Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24ECBD24@vap1014.win.rwjuh.edu>

Yes, more often than you may imagine. Unexpected findings are always called and documented. See ANP12175. Although not required, another reason is the courtesy extended by the pathologist to the physician. This may occur if a preliminary report is to be issued, or the final report not signed out while the pathologist waits for additional tests to be completed. The pathologist may call if he/she knows that the physician is especially interested in getting the results for patient care reasons, and wants to explain why there may be delays to the final report.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie
Sent: Tuesday, September 23, 2014 1:23 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Surgical Pathology reports

Do pathologists ever call pathology reports to a physician?  If so, what types of diagnoses do they call?  There are no "critical" test results in Pathology that CAP deems necessary to call as in other areas of the laboratory..  How do others handle this?


Debra Lake  MT(ASCP)
Manager Micro, Blood Bank, Pathology
Marion General Hospital
Marion, IN  46952
(765) 660-6521
Fax: (765-651-7330)






If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately.
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From joelleweaver <@t> hotmail.com  Tue Sep 23 13:59:59 2014
From: joelleweaver <@t> hotmail.com (Joelle Weaver)
Date: Tue Sep 23 14:00:06 2014
Subject: [Histonet] RE: Surgical Pathology reports
In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24ECBD24@vap1014.win.rwjuh.edu>
References: <96C654E4B0F52748948EFA2938B5DEE2ED3527B3@mghemail02.mgh.net>,
	<59E09A4EFBD3F349BD75FDAE8AFB0F24ECBD24@vap1014.win.rwjuh.edu>
Message-ID: <SNT149-W6423544F5D3C1F3805D7CCD8B00@phx.gbl>

Pretty much " same here". The policy I wrote complies with the ANP checklist for " critical findings", give fairly broad scope the pathologist to determine when this is needed or just good patient care, and provides for documentation when this occurs. 


Joelle Weaver MAOM, HTL (ASCP) QIHC

        
  

 
> From: Toni.Rathborne@rwjuh.edu
> To: Debbie.Lake@mgh.net; histonet@lists.utsouthwestern.edu
> Date: Tue, 23 Sep 2014 17:34:16 +0000
> CC: 
> Subject: [Histonet] RE: Surgical Pathology reports
> 
> Yes, more often than you may imagine. Unexpected findings are always called and documented. See ANP12175. Although not required, another reason is the courtesy extended by the pathologist to the physician. This may occur if a preliminary report is to be issued, or the final report not signed out while the pathologist waits for additional tests to be completed. The pathologist may call if he/she knows that the physician is especially interested in getting the results for patient care reasons, and wants to explain why there may be delays to the final report.
> 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie
> Sent: Tuesday, September 23, 2014 1:23 PM
> To: 'histonet@lists.utsouthwestern.edu'
> Subject: [Histonet] Surgical Pathology reports
> 
> Do pathologists ever call pathology reports to a physician?  If so, what types of diagnoses do they call?  There are no "critical" test results in Pathology that CAP deems necessary to call as in other areas of the laboratory..  How do others handle this?
> 
> 
> Debra Lake  MT(ASCP)
> Manager Micro, Blood Bank, Pathology
> Marion General Hospital
> Marion, IN  46952
> (765) 660-6521
> Fax: (765-651-7330)
> 
> 
> 
> 
> 
> 
> If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately.
> _______________________________________________
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From HornHV <@t> archildrens.org  Tue Sep 23 14:05:13 2014
From: HornHV <@t> archildrens.org (Horn, Hazel V)
Date: Tue Sep 23 14:05:23 2014
Subject: [Histonet] RE: Surgical Pathology reports
In-Reply-To: <96C654E4B0F52748948EFA2938B5DEE2ED3527B3@mghemail02.mgh.net>
References: <96C654E4B0F52748948EFA2938B5DEE2ED3527B3@mghemail02.mgh.net>
Message-ID: <25A4DE08332B19499904459F00AAACB719E29EFF0D@EVS1.archildrens.org>

Debby there is critical test results to call.  Unexpected findings such as malignant when benign were expected for example.   Our pathologists call and it is documented in the pathology report when they called and who they spoke to.   If the clinicians are waiting on a diagnosis and results are delayed for special testing the pathologists will call and tell them what is going on with their case.  There is a CAP standard for this very issue, what you do about unexpected findings.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hornhv@archildrens.org
archildrens.org






-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie
Sent: Tuesday, September 23, 2014 12:23 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Surgical Pathology reports

Do pathologists ever call pathology reports to a physician?  If so, what types of diagnoses do they call?  There are no "critical" test results in Pathology that CAP deems necessary to call as in other areas of the laboratory..  How do others handle this?

Debra Lake  MT(ASCP)
Manager Micro, Blood Bank, Pathology
Marion General Hospital
Marion, IN  46952
(765) 660-6521
Fax: (765-651-7330)






If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately.
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Thank you.

From dmaney22 <@t> yahoo.com  Tue Sep 23 21:19:08 2014
From: dmaney22 <@t> yahoo.com (Donna Maney)
Date: Tue Sep 23 21:19:15 2014
Subject: [Histonet] RE: Artifact in IHC
In-Reply-To: <54206D35.6030504@umdnj.edu>
Message-ID: <1411525148.7104.YahooMailBasic@web125401.mail.ne1.yahoo.com>

Geoff and Terry,
Thanks so much for your input. All of the Histonet posts went into my spam so I just saw these today, but in the meantime by coincidence we ended up following your suggestions. To us, it looked like the tissue was drying out and cracking before being coverslipped. A new technician was being trained, and was perhaps trying too hard to drain all of the xylene off the slide. She noted that the longer she took to coverslip the slide, the worse the artifact was. So we soaked off the coverslips, rehydrated down through 70% and into 50% then water, then dehydrated and coverslipped again. I'd say the artifact is 95% gone. 

So, I don't know if it was just being speedier with the coverslip or the lower concentrations of alcohol and water soak that did the trick. But we will be implementing both now.

Thanks again,
Donna
--------------------------------------------
On Tue, 9/23/14, Geoff <mcauliff@rwjms.rutgers.edu> wrote:

 Subject: Re: [Histonet] RE: Artifact in IHC
 To: histonet@lists.utsouthwestern.edu
 Date: Tuesday, September 23, 2014, 3:40 AM
 
 Teri makes an excellent
 point about higher alcohols causing 
 precipitation of phosphate salts.
 
 Geoff
 
 On 9/22/2014 1:17 PM, Teri Johnson wrote:
 > Hi Donna,
 >
 > Interesting conundrum! A few questions:
 >???- Do you see it in tissues
 that have not undergone any staining, just floated in buffer
 and coverslipped?
 >???- Have
 you tried wet mounting them after staining and prior to
 dehydration and clearing to see if the artifact is there?
 >???- Have you recently changed
 glove manufacturers, could this be talc or some other powder
 from gloves?
 >???- Are you
 dehydrating out of PBS into an alcohol concentration higher
 than 70%? You could be getting phosphate precipitation. Try
 using a water rinse prior to alcohol or make sure your first
 alcohol concentration is 70% or lower.
 >
 > Those are the weird
 things that came to mind. I hope this helps,
 >
 > Teri Johnson
 > Manager Clinical Trial Testing
 > Genoptix, Inc.
 >
 Carlsbad, CA 92008
 >
 >
 >
 >
 ________________________________
 >
 > CONFIDENTIALITY NOTICE: This e-mail
 message, including any attachments, is for the sole use of
 the intended recipient(s) and contains information that is
 confidential and proprietary to Genoptix Medical Laboratory
 or its subsidiaries. Any unauthorized review, use,
 disclosure or distribution is prohibited. If you are not the
 intended recipient, immediately contact the sender by e-mail
 and destroy all copies of the original message.
 >
 >
 >
 _______________________________________________
 > Histonet mailing list
 >
 Histonet@lists.utsouthwestern.edu
 > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 
 -- 
 --
 **********************************************
 Geoff McAuliffe, Ph.D.
 Neuroscience and Cell Biology
 Robert Wood Johnson Medical School
 675 Hoes Lane, Piscataway, NJ 08854
 voice: (732) 235-4583; fax: -4029
 mcauliff@rwjms.rutgers.edu
 **********************************************
 
 
 
 _______________________________________________
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 

From michelecarr10 <@t> gmail.com  Tue Sep 23 23:00:02 2014
From: michelecarr10 <@t> gmail.com (michele carr)
Date: Tue Sep 23 23:00:14 2014
Subject: [Histonet] Mohs
Message-ID: <CABQBf=mdyEjjrizcba-j1M3zO3M2+WnMg+y7Z3mJxf5UwoSx0Q@mail.gmail.com>

Hello I perform mohs cutting a few days a week and I noticed that on fatty
skin tissue I don't get the interior of the skin section. I only seem to
get the epidermis. Is there a trick to getting the fatty tissue too. I
tried bumping up the microns but that only helped a bit.
Thanks for your responses.

Michele Carr
From LSebree <@t> uwhealth.org  Wed Sep 24 07:46:37 2014
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Wed Sep 24 07:46:41 2014
Subject: [Histonet] Light/Heavy chain Myosin to differentiate Type 1 vs Type
 2 muscle fibers
Message-ID: <77DD817201982748BC67D7960F2F76AF0DC7C5@UWHC-MBX12.uwhis.hosp.wisc.edu>

Good morning Histonet,

We are looking for a reference lab to send FFPE slides to for the above antibody(ies).  We sent out for this test several years ago  but of course at that time our records were very rudimentary, i.e. log books,  so difficult to go back and search through.  Hope someone knows something to help us.


Thanks,


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174



From cory.collins <@t> DHAT.com  Wed Sep 24 08:30:22 2014
From: cory.collins <@t> DHAT.com (Cory Collins)
Date: Wed Sep 24 08:30:30 2014
Subject: [Histonet] Histotech Position -DALLAS, TX
Message-ID: <36623916C543B0489F2398E922AAC250744EB8D4@DHMEXCH01.DHAT.com>

We are a large digestive health histology laboratory in Dallas, TX.  We are looking for either an experienced tech or a recent college grad with a science degree to train.  Hours are 7am - 3:30 pm. Competitive benefits and salary. Please email your resume to cory.collins@dhat.com<mailto:cory.collins@dhat.com>.

Thanks,

Cory

Cory Collins, HT (ASCP), QIHC
P: 214-442-5805
F: 214-442-5806





This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the sender and delete this message from your system. Please note that any views or opinions presented in this email are solely those of the author and do not necessarily represent those of the company. Finally, the recipient should check this email and any attachments for the presence of viruses. Digestive Health Management/Digestive Health Associates of Texas accepts no liability for any damage caused by any virus transmitted by this email.

From amurvosh <@t> advancederm.net  Wed Sep 24 08:39:39 2014
From: amurvosh <@t> advancederm.net (Anne Murvosh)
Date: Wed Sep 24 08:39:45 2014
Subject: [Histonet] Mohs
In-Reply-To: <CABQBf=mdyEjjrizcba-j1M3zO3M2+WnMg+y7Z3mJxf5UwoSx0Q@mail.gmail.com>
References: <CABQBf=mdyEjjrizcba-j1M3zO3M2+WnMg+y7Z3mJxf5UwoSx0Q@mail.gmail.com>
Message-ID: <4AD6A4E531E8C943A730559B6B81DF07E8ED07@dc.Advancederm.net>

On fatty tissue, especially cheeks. After we mount on a slide we spray the tissue with liquid nitrogen and let sit to freeze before embedding for a couple of minutes.  Than after embedding spray the chuck again with liquid nitrogen.  On really fatty stuff you may have to freeze before cutting each time. Time consuming yes.  But I find freezing and let sitting I don't fight with it as much when cutting.  Anne

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of michele carr
Sent: Tuesday, September 23, 2014 9:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mohs

Hello I perform mohs cutting a few days a week and I noticed that on fatty skin tissue I don't get the interior of the skin section. I only seem to get the epidermis. Is there a trick to getting the fatty tissue too. I tried bumping up the microns but that only helped a bit.
Thanks for your responses.

Michele Carr
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From relia1 <@t> earthlink.net  Wed Sep 24 09:44:10 2014
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Wed Sep 24 09:44:15 2014
Subject: [Histonet] RELIA Histology Careers Bulletin 9/24/2014 Are you ready
	for Fall?
Message-ID: <004c01cfd806$009d83c0$01d88b40$@earthlink.net>

Hello Histonetters!!
How are you?  Are you enjoying the sights, sounds and flavors of Fall?
I know I am enjoying college football after all I am a southern gal and you
know how we southerners are about our Football Saturdays in the South.  On
the other hand I am still patiently waiting for that "real Fall stuff".  You
know like crisp cool air, beautiful fall colors, crunching leaves and
steaming bowls of soup. Because it is still 90 degrees here in Orlando!!!!
 
 What are you enjoying most of all about this season?
 
I also wanted to tell you about some new job opportunities.  
These are some of my best clients and they are ready to interview and hire
right away.
 
HOT HISTOLOGY OPENINGS:
AP Manager - East of Chicago
Dermpath Histotech - Fayetteville, AR
Histotechnologist - Philadelphia area
Histotechnician - Modesto, CA
Histotechnician - Mountain View, CA
Senior/Lead Histotech - St. Louis
Histotech - Norfolk, VA
Histotech - Williamsburg, VA
 
If you are interested in participating in my referral program in addition to
these histology openings I also need cytotechs in NC and LA.
 
If you or anyone you know might be interested in any of these opportunities
or would like help with a job search in another area of the USA please
contact me.  
I can be reached at relia1@earthlink.net or toll free at 866-607-3542.
Thanks-Pam


Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net 
www.facebook.com <http://www.facebook.com/PamBarkerRELIA> /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 





From LRaff <@t> uropartners.com  Wed Sep 24 10:43:29 2014
From: LRaff <@t> uropartners.com (Lester Raff MD)
Date: Wed Sep 24 10:43:34 2014
Subject: [Histonet] Floor Tiles
Message-ID: <FE0368FE5AA6684DB5990BBEED742C5302F88A8A@UPHQMSX01.uropartners.local>

Our entire lab, including histology, has linoleum flooring. We cover
areas with non-skid mats as necessary. 

 

We will be renegotiating our lease shortly and are considering having
the building management company replace the flooring. Are there are any
recommendations for a superior, non-slip, easy to clean and maintain
flooring for the lab, particularly histology?

 

Thanks,

 

Lester J. Raff, MD MBA

UroPartners

Medical Director Of Laboratory

2225 Enterprise Dr. Suite 2511

Westchester, Il 60154

Tel: 708-486-0076

Fax: 708-492-0203

 

From langleykristi <@t> hotmail.com  Wed Sep 24 10:44:12 2014
From: langleykristi <@t> hotmail.com (Kristi Langley)
Date: Wed Sep 24 10:44:15 2014
Subject: [Histonet] H.Pylori Control/AFP
Message-ID: <BLU175-W407E0838E871676994DBBA5B10@phx.gbl>

Does anyone have any h-pylori control blocks they would like to share? Also looking for AFP...Willing to trade:) We have many other kinds of tissue...Just running short on those.... Thanks

Kristi Langley HT(ASCP)

Converge Dx.

Peabody, Mass.

 
 		 	   		  
From blayjorge <@t> gmail.com  Wed Sep 24 11:06:02 2014
From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay)
Date: Wed Sep 24 11:06:08 2014
Subject: [Histonet] Gram stain not working consistently: how likely is it
 that crystal violet has gone "bad"
Message-ID: <CAGBdDuB68aybsS-3AKDxG0mOYQiNLohDR3_K0zcxHafNxREzow@mail.gmail.com>

Dear Histoneters:

If Gram stains are not always working consistently: how likely is it that
crystal violet has gone "bad". My first suspect is the decoloration step,
then age of the culture, then thickness of the smear.  However, I do not
want to disregard any possibility, such as bad reagents. Please, email any
feedback directly to me, blayjorge@gmail.com.

Cheers and gracias,

Jorge

Jorge A. Santiago-Blay, PhD
blaypublishers.com
http://blayjorge.wordpress.com/
http://paleobiology.si.edu/staff/individuals/santiagoblay.html
From Timothy.Morken <@t> ucsfmedctr.org  Wed Sep 24 11:39:50 2014
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy)
Date: Wed Sep 24 11:40:06 2014
Subject: [Histonet] RE: Floor Tiles
In-Reply-To: <FE0368FE5AA6684DB5990BBEED742C5302F88A8A@UPHQMSX01.uropartners.local>
References: <FE0368FE5AA6684DB5990BBEED742C5302F88A8A@UPHQMSX01.uropartners.local>
Message-ID: <761E2B5697F795489C8710BCC72141FF3679A142@ex07.net.ucsf.edu>

I don't think the ideal exists. Anything that is flat is going to have some slip issues, though the right floor wax can help, and maybe those peel-off sticky mats to catch dirt/paraffin. 

We have a vinyl tile floor that has a grid pattern embossed into it - 1" squares. It is pretty much non-slip, but wax cannot be scraped off easily because of the embossed indentations. We need housekeeping to do some serious machine stripping and rewaxing every two weeks to clean it.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD
Sent: Wednesday, September 24, 2014 8:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Floor Tiles

Our entire lab, including histology, has linoleum flooring. We cover areas with non-skid mats as necessary. 

 

We will be renegotiating our lease shortly and are considering having the building management company replace the flooring. Are there are any recommendations for a superior, non-slip, easy to clean and maintain flooring for the lab, particularly histology?

 

Thanks,

 

Lester J. Raff, MD MBA

UroPartners

Medical Director Of Laboratory

2225 Enterprise Dr. Suite 2511

Westchester, Il 60154

Tel: 708-486-0076

Fax: 708-492-0203

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From HornHV <@t> archildrens.org  Wed Sep 24 11:43:34 2014
From: HornHV <@t> archildrens.org (Horn, Hazel V)
Date: Wed Sep 24 11:43:38 2014
Subject: [Histonet] RE: Floor Tiles
In-Reply-To: <761E2B5697F795489C8710BCC72141FF3679A142@ex07.net.ucsf.edu>
References: <FE0368FE5AA6684DB5990BBEED742C5302F88A8A@UPHQMSX01.uropartners.local>
	<761E2B5697F795489C8710BCC72141FF3679A142@ex07.net.ucsf.edu>
Message-ID: <25A4DE08332B19499904459F00AAACB719E29EFF12@EVS1.archildrens.org>

We have a non wax floor.  It's a flat surface and easy to clean.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hornhv@archildrens.org
archildrens.org






-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, September 24, 2014 11:40 AM
To: 'Lester Raff MD'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Floor Tiles

I don't think the ideal exists. Anything that is flat is going to have some slip issues, though the right floor wax can help, and maybe those peel-off sticky mats to catch dirt/paraffin. 

We have a vinyl tile floor that has a grid pattern embossed into it - 1" squares. It is pretty much non-slip, but wax cannot be scraped off easily because of the embossed indentations. We need housekeeping to do some serious machine stripping and rewaxing every two weeks to clean it.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD
Sent: Wednesday, September 24, 2014 8:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Floor Tiles

Our entire lab, including histology, has linoleum flooring. We cover areas with non-skid mats as necessary. 

 

We will be renegotiating our lease shortly and are considering having the building management company replace the flooring. Are there are any recommendations for a superior, non-slip, easy to clean and maintain flooring for the lab, particularly histology?

 

Thanks,

 

Lester J. Raff, MD MBA

UroPartners

Medical Director Of Laboratory

2225 Enterprise Dr. Suite 2511

Westchester, Il 60154

Tel: 708-486-0076

Fax: 708-492-0203

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

******************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************
The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the 
intended recipient, or an employee or agent responsible for delivering this 
message to the intended recipient, you are hereby notified that any 
dissemination, distribution or copying of this communication is strictly 
prohibited. If you have received this communication in error, please notify 
us immediately by replying to the message and deleting it from your computer.
Thank you.

From suetp918 <@t> comcast.net  Wed Sep 24 11:44:02 2014
From: suetp918 <@t> comcast.net (Sue)
Date: Wed Sep 24 11:44:12 2014
Subject: [Histonet] H.Pylori Control/AFP
In-Reply-To: <BLU175-W407E0838E871676994DBBA5B10@phx.gbl>
References: <BLU175-W407E0838E871676994DBBA5B10@phx.gbl>
Message-ID: <727372632.4802516.1411577042677.JavaMail.root@comcast.net>

HP is difficult, we actually use a patien control from a positive case and cut approx 20 slides from each block.? You can probably purchase them but they are costly. 
? 
Sue Paturzo 
TJUH 
From PAMarcum <@t> uams.edu  Wed Sep 24 11:49:27 2014
From: PAMarcum <@t> uams.edu (Marcum, Pamela A)
Date: Wed Sep 24 11:49:37 2014
Subject: [Histonet] RE: Floor Tiles
In-Reply-To: <25A4DE08332B19499904459F00AAACB719E29EFF12@EVS1.archildrens.org>
References: <FE0368FE5AA6684DB5990BBEED742C5302F88A8A@UPHQMSX01.uropartners.local>
	<761E2B5697F795489C8710BCC72141FF3679A142@ex07.net.ucsf.edu>
	<25A4DE08332B19499904459F00AAACB719E29EFF12@EVS1.archildrens.org>
Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32012526C2DD@Mail2Node2.ad.uams.edu>

We have really old floor tiles and do not allow Housekeeping to wax them with anything that would be slippery.  They use a non-skid wax only if they use anything.  

Pam Marcum

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Wednesday, September 24, 2014 11:44 AM
To: 'Morken, Timothy'; 'Lester Raff MD'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Floor Tiles

We have a non wax floor.  It's a flat surface and easy to clean.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hornhv@archildrens.org
archildrens.org






-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, September 24, 2014 11:40 AM
To: 'Lester Raff MD'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Floor Tiles

I don't think the ideal exists. Anything that is flat is going to have some slip issues, though the right floor wax can help, and maybe those peel-off sticky mats to catch dirt/paraffin. 

We have a vinyl tile floor that has a grid pattern embossed into it - 1" squares. It is pretty much non-slip, but wax cannot be scraped off easily because of the embossed indentations. We need housekeeping to do some serious machine stripping and rewaxing every two weeks to clean it.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD
Sent: Wednesday, September 24, 2014 8:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Floor Tiles

Our entire lab, including histology, has linoleum flooring. We cover areas with non-skid mats as necessary. 

 

We will be renegotiating our lease shortly and are considering having the building management company replace the flooring. Are there are any recommendations for a superior, non-slip, easy to clean and maintain flooring for the lab, particularly histology?

 

Thanks,

 

Lester J. Raff, MD MBA

UroPartners

Medical Director Of Laboratory

2225 Enterprise Dr. Suite 2511

Westchester, Il 60154

Tel: 708-486-0076

Fax: 708-492-0203

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

******************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************
The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer.
Thank you.

_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.

From KSimeone <@t> leavittmgt.com  Wed Sep 24 12:20:59 2014
From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone)
Date: Wed Sep 24 12:21:10 2014
Subject: [Histonet] FT NIGHT SHIFT DELRAY BEACH FL 
Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C2FDA365B@vm-email.leavittmgt.com>

Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE.



***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!



Email your resume to lengimann@leavittmgt.com<mailto:lengimann@leavittmgt.com> if interested.



*full time position Mon-Fri PM (will discuss hours upon interview/will be AFTER 5p)

*MUST be licensed as a FL histotechnologist or technician

*MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE!

*VERY proficient in embedding and microtomy

*must be self motivated, reliable and a team player

*knowledge in operating Ventana and Leica equipment desired (not necessary)



Kari M Simeone

561.819.6517 fax

ksimeone@leavittmgt.com<mailto:ksimeone@leavittmgt.com>

www.advancedderm.com<http://www.advancedderm.com/>



The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message.


From tbraud <@t> holyredeemer.com  Wed Sep 24 12:25:09 2014
From: tbraud <@t> holyredeemer.com (Terri  Braud)
Date: Wed Sep 24 12:25:16 2014
Subject: [Histonet] RE: Histonet Digest, Vol 130, Issue 24
In-Reply-To: <20140924160906.ED2D11E838E@trendmess-svr.holyredeemer.local>
References: <20140924160906.ED2D11E838E@trendmess-svr.holyredeemer.local>
Message-ID: <BFBF7C6B9627B947B74CBEBD45CE368B144957C9@hrex-svr.holyredeemer.local>

1.  Our AP critical report list includes: frozen section diagnosis,
gross intra-operative consult, intra-operative cytology for immediate
assessment, cytology FNA immediate evaluation, new malignancies, major
amended reports, inadequate specimens or empty containers, unusual or
unexpected findings (these are also defined), clinical history and
procedure do not match.
Our pathologists always make note of date/time/who they talked to/topic
Documented within the body of the report.
Sincerely, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874
Today's Topics:

   1. Surgical Pathology reports (Lake,Debbie)
Message: 1
Date: Tue, 23 Sep 2014 17:22:35 +0000
From: "Lake,Debbie" <Debbie.Lake@mgh.net>
Subject: [Histonet] Surgical Pathology reports


Do pathologists ever call pathology reports to a physician?  If so, what
types of diagnoses do they call?  There are no "critical" test results
in Pathology that CAP deems necessary to call as in other areas of the
laboratory..  How do others handle this?

Debra Lake  MT(ASCP)
Manager Micro, Blood Bank, Pathology
Marion General Hospital
Marion, IN  46952
(765) 660-6521
Fax: (765-651-7330)

*****************
---------------------------------------------------------------------------------



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From kaarrington <@t> anthc.org  Wed Sep 24 12:43:45 2014
From: kaarrington <@t> anthc.org (Arrington, Karla A)
Date: Wed Sep 24 12:43:50 2014
Subject: [Histonet] FDA Disclaimer
Message-ID: <F362122314DD5C41BD407A39C4FCF8757B9BA275@AKHV-EX2K12MB3.alaska.ihs.gov>

To All:

We are starting up IHC at the lab and I have a question about disclaimer.

If we are using all FDA approved antibodies, do we need the FDA Disclaimer on our pathology reports?
And if so, what should it say?

Karla Arrington =)

Karla Arrington, HT(ASCP), HIT(AHIMA)
Lead Histology Technician
ANMC Pathology
907-729-1810
kaarrington@anthc.org

From Timothy.Morken <@t> ucsfmedctr.org  Wed Sep 24 13:24:11 2014
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy)
Date: Wed Sep 24 13:24:27 2014
Subject: [Histonet] RE: FDA Disclaimer
In-Reply-To: <F362122314DD5C41BD407A39C4FCF8757B9BA275@AKHV-EX2K12MB3.alaska.ihs.gov>
References: <F362122314DD5C41BD407A39C4FCF8757B9BA275@AKHV-EX2K12MB3.alaska.ihs.gov>
Message-ID: <761E2B5697F795489C8710BCC72141FF3679A1D0@ex07.net.ucsf.edu>

Karla, the thing is, they may be called "IVD" under FDA nomenclature,  but they are not all FDA "approved." Class I exempt antibodies  (the vast majority) don't need FDA "approval" because they are ancillary tests used in conjunction with other tests to arrive at a Dx.  Only stand-alone tests like ER, Pr, Her2 and EGFR are in Class II and require FDA approval.

An FDA certified company can list any antibody (except the stand alones) as IVD Class I simply by submitting a list to FDA. The company must have FDA approved quality controls in place and follow a slew of regulations to do so, but it is simply a paperwork exercise after that. 

So, the disclaimer you mention says that the antibody in question is not FDA approved, but is not required to be FDA approved. The CAP and ASCP suggested this disclaimer so that customers would be made to understand that although the tests are not under FDA approval it does not mean they are not valid tests. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arrington, Karla A
Sent: Wednesday, September 24, 2014 10:44 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] FDA Disclaimer

To All:

We are starting up IHC at the lab and I have a question about disclaimer.

If we are using all FDA approved antibodies, do we need the FDA Disclaimer on our pathology reports?
And if so, what should it say?

Karla Arrington =)

Karla Arrington, HT(ASCP), HIT(AHIMA)
Lead Histology Technician
ANMC Pathology
907-729-1810
kaarrington@anthc.org

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From joelleweaver <@t> hotmail.com  Wed Sep 24 14:14:13 2014
From: joelleweaver <@t> hotmail.com (Joelle Weaver)
Date: Wed Sep 24 14:14:19 2014
Subject: [Histonet] RE: FDA Disclaimer
In-Reply-To: <761E2B5697F795489C8710BCC72141FF3679A1D0@ex07.net.ucsf.edu>
References: <F362122314DD5C41BD407A39C4FCF8757B9BA275@AKHV-EX2K12MB3.alaska.ihs.gov>,
	<761E2B5697F795489C8710BCC72141FF3679A1D0@ex07.net.ucsf.edu>
Message-ID: <SNT149-W19F0A5AE760C232E01561BD8B10@phx.gbl>

Tim has aleady supplied alot of information here.  When I needed the disclaimer, I used the language and wording  directly from the ANP checklist placed in quotations within the reports when I put together the basic templates in LIS. Depending on your system, you might be able to link it to the test mneumonic or other means to drop it in automatically when this test is ordered or reported in the final pathology report. 
       


Joelle Weaver MAOM, HTL (ASCP) QIHC

        
  

 
> From: Timothy.Morken@ucsfmedctr.org
> To: kaarrington@anthc.org; histonet@lists.utsouthwestern.edu
> Date: Wed, 24 Sep 2014 18:24:11 +0000
> CC: 
> Subject: [Histonet] RE: FDA Disclaimer
> 
> Karla, the thing is, they may be called "IVD" under FDA nomenclature,  but they are not all FDA "approved." Class I exempt antibodies  (the vast majority) don't need FDA "approval" because they are ancillary tests used in conjunction with other tests to arrive at a Dx.  Only stand-alone tests like ER, Pr, Her2 and EGFR are in Class II and require FDA approval.
> 
> An FDA certified company can list any antibody (except the stand alones) as IVD Class I simply by submitting a list to FDA. The company must have FDA approved quality controls in place and follow a slew of regulations to do so, but it is simply a paperwork exercise after that. 
> 
> So, the disclaimer you mention says that the antibody in question is not FDA approved, but is not required to be FDA approved. The CAP and ASCP suggested this disclaimer so that customers would be made to understand that although the tests are not under FDA approval it does not mean they are not valid tests. 
> 
> Tim Morken
> Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
> UC San Francisco Medical Center
> San Francisco, CA
> 
> CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.
> 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arrington, Karla A
> Sent: Wednesday, September 24, 2014 10:44 AM
> To: 'histonet@lists.utsouthwestern.edu'
> Subject: [Histonet] FDA Disclaimer
> 
> To All:
> 
> We are starting up IHC at the lab and I have a question about disclaimer.
> 
> If we are using all FDA approved antibodies, do we need the FDA Disclaimer on our pathology reports?
> And if so, what should it say?
> 
> Karla Arrington =)
> 
> Karla Arrington, HT(ASCP), HIT(AHIMA)
> Lead Histology Technician
> ANMC Pathology
> 907-729-1810
> kaarrington@anthc.org
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From Robert.Fauck <@t> ccdhb.org.nz  Wed Sep 24 15:28:28 2014
From: Robert.Fauck <@t> ccdhb.org.nz (Robert Fauck [CCDHB])
Date: Wed Sep 24 15:28:46 2014
Subject: [Histonet] Unsubscribe me please
Message-ID: <A4BC28704CC61446B957A5B4BDDF24361529C4@wn0nteml13.AD.CCDHB.HEALTH.NZ>

Hi
 
Could you please unsubscribe me from your Histo-List.
 
Thanks
 
Robert Fauck


This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board.

http://www.ccdhb.org.nz

(1C_S1)
 
From Joyce.Weems <@t> emoryhealthcare.org  Wed Sep 24 15:34:29 2014
From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.)
Date: Wed Sep 24 15:34:39 2014
Subject: [Histonet] RE: Unsubscribe me please
In-Reply-To: <A4BC28704CC61446B957A5B4BDDF24361529C4@wn0nteml13.AD.CCDHB.HEALTH.NZ>
References: <A4BC28704CC61446B957A5B4BDDF24361529C4@wn0nteml13.AD.CCDHB.HEALTH.NZ>
Message-ID: <E3A4EBD57A691646BCCED4AA5911A030011C34D798@e14mbx12n.Enterprise.emory.net>

Hi Robert,

You must do that at http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Thanks and best wishes!


Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s).  It may contain information that is privileged and confidential.  Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Fauck [CCDHB]
Sent: Wednesday, September 24, 2014 4:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Unsubscribe me please

Hi

Could you please unsubscribe me from your Histo-List.

Thanks

Robert Fauck


This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board.

http://www.ccdhb.org.nz

(1C_S1)

_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

________________________________

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the intended recipient(s) and may contain confidential and privileged
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From LSebree <@t> uwhealth.org  Wed Sep 24 15:45:48 2014
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Wed Sep 24 15:45:53 2014
Subject: [Histonet] RE: FDA Disclaimer
In-Reply-To: <761E2B5697F795489C8710BCC72141FF3679A1D0@ex07.net.ucsf.edu>
References: <F362122314DD5C41BD407A39C4FCF8757B9BA275@AKHV-EX2K12MB3.alaska.ihs.gov>
	<761E2B5697F795489C8710BCC72141FF3679A1D0@ex07.net.ucsf.edu>
Message-ID: <77DD817201982748BC67D7960F2F76AF0DC942@UWHC-MBX12.uwhis.hosp.wisc.edu>

Well put Tim.

Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, September 24, 2014 1:24 PM
To: 'Arrington, Karla A'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: FDA Disclaimer

Karla, the thing is, they may be called "IVD" under FDA nomenclature,  but they are not all FDA "approved." Class I exempt antibodies  (the vast majority) don't need FDA "approval" because they are ancillary tests used in conjunction with other tests to arrive at a Dx.  Only stand-alone tests like ER, Pr, Her2 and EGFR are in Class II and require FDA approval.

An FDA certified company can list any antibody (except the stand alones) as IVD Class I simply by submitting a list to FDA. The company must have FDA approved quality controls in place and follow a slew of regulations to do so, but it is simply a paperwork exercise after that. 

So, the disclaimer you mention says that the antibody in question is not FDA approved, but is not required to be FDA approved. The CAP and ASCP suggested this disclaimer so that customers would be made to understand that although the tests are not under FDA approval it does not mean they are not valid tests. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arrington, Karla A
Sent: Wednesday, September 24, 2014 10:44 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] FDA Disclaimer

To All:

We are starting up IHC at the lab and I have a question about disclaimer.

If we are using all FDA approved antibodies, do we need the FDA Disclaimer on our pathology reports?
And if so, what should it say?

Karla Arrington =)

Karla Arrington, HT(ASCP), HIT(AHIMA)
Lead Histology Technician
ANMC Pathology
907-729-1810
kaarrington@anthc.org

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From annigyg <@t> gmail.com  Wed Sep 24 21:57:26 2014
From: annigyg <@t> gmail.com (Anne)
Date: Wed Sep 24 21:57:38 2014
Subject: [Histonet] New Artisan Gram stain kits
Message-ID: <D67F43E1-E724-4B61-922E-31F85AD87599@gmail.com>

Hello there you awesome group of histo gurus...
We have been running the new improved Artisan Gram kits.
They say to use the standard on board staining protocol. This gives us no gram+ orgs. 
We reset the CV to its max. Still no blue. 
We used the same new kit reagents manually with the same instrument timings. Perfect results!!
Tried to decrease the on board decolorisation step. Nothing. 
So for now we do them all manually. 
Hope someone out there has some wise words for me. 
Greetings from the sandpit
Annie (still from, but not IN, Africa)

Sent from my iPhone
From Ronald.Houston <@t> nationwidechildrens.org  Thu Sep 25 08:06:28 2014
From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald)
Date: Thu Sep 25 08:06:37 2014
Subject: [Histonet] Dragon Voice Recognition software
Message-ID: <E5D0CD2352E46545A0C0EBE308CCCE53342384F1@L1PERDWXMB02.childrensroot.net>

Is anyone using this transcribing directly into Sunquest CoPathPlus?

How successful was the transition, what problems did you encounter, and what resistance if any was there from pathologists, PAs and transcriptionists?

Thanks

Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston@nationwidechildrens.org<mailto:ronald.houston@nationwidechildrens.org>
www.NationwideChildrens.org<http://www.nationwidechildrens.org/>

"One person with passion is better than forty people merely interested."
~ E.M. Forster


From judi <@t> medialabinc.net  Thu Sep 25 13:22:35 2014
From: judi <@t> medialabinc.net (Judi Bennett)
Date: Thu Sep 25 13:22:39 2014
Subject: [Histonet] Seeking HT/HTL Program Directors to Write Histology
	Questions
Message-ID: <CAC2QUZ-zt8QupSJRhP4+bDLzVYWQQiPc7PyJZhVm+kjLAzsJPQ@mail.gmail.com>

Actively seeking *HT/HTL Program Directors* to write* histology questions*
for *MediaLab*!  MediaLab is a leading publisher of online continuing
education (CE) courses, competency assessments, and student preparation
tools. Our online products are used at more than 2,000 laboratories and
university CLS/HT/HTL programs worldwide.

This is a great opportunity to be involved in creating questions to help
HT/HTL students prepare for their ASCP Board of Certification exam and *earn
honorariums* for your participation. The certification examination is
challenging and requires thoughtful preparation. These histology questions,
developed by MediaLab, will be featured in our* Histology Exam Simulator*.

Authors can take advantage of MediaLab's online CourseBuilder to *write
questions anytime, anywhere*. CourseBuilder is easy to use, with an
intuitive interface similar to Microsoft Word or PowerPoint. Authors can
quickly create questions and upload relevant images.

To learn more about becoming a MediaLab author and writing histology
questions, please contact Judi Bennett at judi@medialabinc.net  or
call 877-776-8460,
ext. 721.

Thank you,

Judi


Judi Bennett, MT, BSM

Program Director - MediaLab, Inc.
e-mail judi@medialabinc.net
Phone (877) 776-8460 ext. 721

cell phone 404-915-2999
fax (678) 401-0284
From judi <@t> medialabinc.net  Thu Sep 25 13:30:16 2014
From: judi <@t> medialabinc.net (Judi Bennett)
Date: Thu Sep 25 13:30:22 2014
Subject: [Histonet] MediaLab is Looking for Histology Course Authors
Message-ID: <CAC2QUZ_+Ky6FNGx2ptU9Wopjp4NX7=g-J2N+SLNT2ubS325s=A@mail.gmail.com>

Actively seeking authors to write and review online *histology courses* for
*MediaLab*!  MediaLab is a leading publisher of online continuing education
(CE) courses and competency assessments. Our online products are used at
more than 2,000 laboratories and university CLS/HT/HTL programs worldwide.

This is a great opportunity to *gain resume-boosting publishing
experience, **earn honorariums* for your participation, and fill the *need to
provide quality histology CE credits*.

Authors can take advantage of MediaLab's online CourseBuilder to *write
courses anytime, anywhere*. CourseBuilder is easy to use, with an intuitive
interface similar to Microsoft Word or PowerPoint. Authors can quickly
create content pages, practice questions, and exam questions, and upload
relevant images.

Courses developed by MediaLab are *featured on our websites MediaLabInc.net
and LabCE.com*. Questions from these courses also become part of the
LabCE.com Quiz Game, with over 1,000 daily players. Authors and reviewers
may also*contribute to other online programs that we develop on behalf of
major laboratory partners*.

To learn more about becoming a MediaLab author for histology courses, visit
our online information page atwww.medialbinc.net/authors.aspx .  Please
contact Judi Bennett at judi@medialabinc.net  or call 877-776-8460, ext. 721
.
Thank you,
Judi


Judi Bennett, MT, BSM

Program Director - MediaLab, Inc.
e-mail judi@medialabinc.net
Phone (877) 776-8460 ext. 721

cell phone 404-915-2999
fax (678) 401-0284
From akelley <@t> path.wustl.edu  Thu Sep 25 13:40:56 2014
From: akelley <@t> path.wustl.edu (Kelley, Amanda)
Date: Thu Sep 25 13:41:04 2014
Subject: [Histonet] MediaLab is Looking for Histology Course Authors
In-Reply-To: <CAC2QUZ_+Ky6FNGx2ptU9Wopjp4NX7=g-J2N+SLNT2ubS325s=A@mail.gmail.com>
References: <CAC2QUZ_+Ky6FNGx2ptU9Wopjp4NX7=g-J2N+SLNT2ubS325s=A@mail.gmail.com>
Message-ID: <F053AFDDBC94524998697F264BEC3EB31974F70F@pathmbx02.wusm-path.wustl.edu>

Judi,
        CLS programs (ASCLS) does NOT offer HT or HTL certification Through their accreditation agency. They don't recognize Histotech's as technicians in California. ASCLS is the creator and sponsor of PACE credits. Is this accrediting agency finally recognizing HT and HTL's as laboratory professionals?  Usually it is only Medical technologists or CLS. Will these PACE credits be acceptable to the only accrediting agency for Histotech's which at this time is ASCP?

Amanda

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judi Bennett
Sent: Thursday, September 25, 2014 1:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] MediaLab is Looking for Histology Course Authors

Actively seeking authors to write and review online *histology courses* for *MediaLab*!  MediaLab is a leading publisher of online continuing education
(CE) courses and competency assessments. Our online products are used at more than 2,000 laboratories and university CLS/HT/HTL programs worldwide.

This is a great opportunity to *gain resume-boosting publishing experience, **earn honorariums* for your participation, and fill the *need to provide quality histology CE credits*.

Authors can take advantage of MediaLab's online CourseBuilder to *write courses anytime, anywhere*. CourseBuilder is easy to use, with an intuitive interface similar to Microsoft Word or PowerPoint. Authors can quickly create content pages, practice questions, and exam questions, and upload relevant images.

Courses developed by MediaLab are *featured on our websites MediaLabInc.net and LabCE.com*. Questions from these courses also become part of the LabCE.com Quiz Game, with over 1,000 daily players. Authors and reviewers may also*contribute to other online programs that we develop on behalf of major laboratory partners*.

To learn more about becoming a MediaLab author for histology courses, visit our online information page atwww.medialbinc.net/authors.aspx .  Please contact Judi Bennett at judi@medialabinc.net  or call 877-776-8460, ext. 721 .
Thank you,
Judi


Judi Bennett, MT, BSM

Program Director - MediaLab, Inc.
e-mail judi@medialabinc.net
Phone (877) 776-8460 ext. 721

cell phone 404-915-2999
fax (678) 401-0284
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
From akelley <@t> path.wustl.edu  Thu Sep 25 14:09:10 2014
From: akelley <@t> path.wustl.edu (Kelley, Amanda)
Date: Thu Sep 25 14:09:19 2014
Subject: [Histonet] MediaLab is Looking for Histology Course Authors
In-Reply-To: <CAC2QUZ9AGm=rg+gtEbN3dr6h8e945-Q2DsB3aVMd7+hTR4X34g@mail.gmail.com>
References: <CAC2QUZ_+Ky6FNGx2ptU9Wopjp4NX7=g-J2N+SLNT2ubS325s=A@mail.gmail.com>
	<F053AFDDBC94524998697F264BEC3EB31974F70F@pathmbx02.wusm-path.wustl.edu>
	<CAC2QUZ9AGm=rg+gtEbN3dr6h8e945-Q2DsB3aVMd7+hTR4X34g@mail.gmail.com>
Message-ID: <F053AFDDBC94524998697F264BEC3EB31974F878@pathmbx02.wusm-path.wustl.edu>

Judi
Do you think it is fair to NSH, and ASCP (which recognize histotech's as professionals) to offer PACE credits to the very technicians that ASCLS refuses to acknowledge as professionals.  ASCLS has a bill in the California legislature which will designate Histotechnicians and Histotechnologists as non -licensed technicians.  So they will fall in a category which does not recognize them as professionals.  Why should Histotechnicians support a group who does not represent them?  Change the CEU's to NSH accredited or ASCP accredited, they recognize us. Do not use ASCLS, to certify this program.  They do NOT represent histotech's.
Amanda

From: Judi Bennett [mailto:judi@medialabinc.net]
Sent: Thursday, September 25, 2014 1:56 PM
To: Kelley, Amanda
Subject: Re: [Histonet] MediaLab is Looking for Histology Course Authors

Hi Amanda,

I'm sorry if I didn't make the wording clear enough. Our online products are available in CLS and HT and HTL programs. I did not mean to insinuate that CLS programs offered HT or HTL certifications. You're right that ASCLS is the provider of PACE credits. I'm also aware that ASCP is the only accrediting agency for histology. ASCP absolutely does accept PACE credits.

I hope that answers your questions - Judi

On Thu, Sep 25, 2014 at 2:40 PM, Kelley, Amanda <akelley@path.wustl.edu<mailto:akelley@path.wustl.edu>> wrote:
Judi,
        CLS programs (ASCLS) does NOT offer HT or HTL certification Through their accreditation agency. They don't recognize Histotech's as technicians in California. ASCLS is the creator and sponsor of PACE credits. Is this accrediting agency finally recognizing HT and HTL's as laboratory professionals?  Usually it is only Medical technologists or CLS. Will these PACE credits be acceptable to the only accrediting agency for Histotech's which at this time is ASCP?

Amanda

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu<mailto:histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu<mailto:histonet-bounces@lists.utsouthwestern.edu>] On Behalf Of Judi Bennett
Sent: Thursday, September 25, 2014 1:30 PM
To: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>
Subject: [Histonet] MediaLab is Looking for Histology Course Authors

Actively seeking authors to write and review online *histology courses* for *MediaLab*!  MediaLab is a leading publisher of online continuing education
(CE) courses and competency assessments. Our online products are used at more than 2,000 laboratories and university CLS/HT/HTL programs worldwide.

This is a great opportunity to *gain resume-boosting publishing experience, **earn honorariums* for your participation, and fill the *need to provide quality histology CE credits*.

Authors can take advantage of MediaLab's online CourseBuilder to *write courses anytime, anywhere*. CourseBuilder is easy to use, with an intuitive interface similar to Microsoft Word or PowerPoint. Authors can quickly create content pages, practice questions, and exam questions, and upload relevant images.

Courses developed by MediaLab are *featured on our websites MediaLabInc.net and LabCE.com*. Questions from these courses also become part of the LabCE.com Quiz Game, with over 1,000 daily players. Authors and reviewers may also*contribute to other online programs that we develop on behalf of major laboratory partners*.

To learn more about becoming a MediaLab author for histology courses, visit our online information page atwww.medialbinc.net/authors.aspx<http://atwww.medialbinc.net/authors.aspx> .  Please contact Judi Bennett at judi@medialabinc.net<mailto:judi@medialabinc.net>  or call 877-776-8460, ext. 721<tel:877-776-8460%2C%20ext.%20721> .
Thank you,
Judi


Judi Bennett, MT, BSM

Program Director - MediaLab, Inc.
e-mail judi@medialabinc.net<mailto:judi@medialabinc.net>
Phone (877) 776-8460 ext. 721<tel:%28877%29%20776-8460%20ext.%20721>

cell phone 404-915-2999<tel:404-915-2999>
fax (678) 401-0284<tel:%28678%29%20401-0284>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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--

Thanks,

Judi



Judi Bennett, MT, BSM

Program Director - MediaLab, Inc.
e-mail judi@medialabinc.net<mailto:judi@medialabinc.net>
Phone (877) 776-8460 ext. 721

cell phone 404-915-2999
fax (678) 401-0284

From joelleweaver <@t> hotmail.com  Thu Sep 25 14:33:09 2014
From: joelleweaver <@t> hotmail.com (Joelle Weaver)
Date: Thu Sep 25 14:33:20 2014
Subject: [Histonet] MediaLab is Looking for Histology Course Authors
In-Reply-To: <CAC2QUZ_+Ky6FNGx2ptU9Wopjp4NX7=g-J2N+SLNT2ubS325s=A@mail.gmail.com>
References: <CAC2QUZ_+Ky6FNGx2ptU9Wopjp4NX7=g-J2N+SLNT2ubS325s=A@mail.gmail.com>
Message-ID: <SNT149-W724F3A2B3498DC5381D20CD8BE0@phx.gbl>

I wanted to say that when I have worked with Medilab on histology related courses in the past that it has been a great experience for me. I highly recommend it as a great learning opportunity for anyone who is interested in working with education, development and training in their practice area.  I just wanted to give some first hand feedback, and encourage anyone who may have the interest or inclination to consider becoming an author or reviewer.  
 
 
Joelle Weaver MAOM, HTL (ASCP) QIHC

        
  

 
> Date: Thu, 25 Sep 2014 14:30:16 -0400
> From: judi@medialabinc.net
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] MediaLab is Looking for Histology Course Authors
> 
> Actively seeking authors to write and review online *histology courses* for
> *MediaLab*!  MediaLab is a leading publisher of online continuing education
> (CE) courses and competency assessments. Our online products are used at
> more than 2,000 laboratories and university CLS/HT/HTL programs worldwide.
> 
> This is a great opportunity to *gain resume-boosting publishing
> experience, **earn honorariums* for your participation, and fill the *need to
> provide quality histology CE credits*.
> 
> Authors can take advantage of MediaLab's online CourseBuilder to *write
> courses anytime, anywhere*. CourseBuilder is easy to use, with an intuitive
> interface similar to Microsoft Word or PowerPoint. Authors can quickly
> create content pages, practice questions, and exam questions, and upload
> relevant images.
> 
> Courses developed by MediaLab are *featured on our websites MediaLabInc.net
> and LabCE.com*. Questions from these courses also become part of the
> LabCE.com Quiz Game, with over 1,000 daily players. Authors and reviewers
> may also*contribute to other online programs that we develop on behalf of
> major laboratory partners*.
> 
> To learn more about becoming a MediaLab author for histology courses, visit
> our online information page atwww.medialbinc.net/authors.aspx .  Please
> contact Judi Bennett at judi@medialabinc.net  or call 877-776-8460, ext. 721
> .
> Thank you,
> Judi
> 
> 
> Judi Bennett, MT, BSM
> 
> Program Director - MediaLab, Inc.
> e-mail judi@medialabinc.net
> Phone (877) 776-8460 ext. 721
> 
> cell phone 404-915-2999
> fax (678) 401-0284
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From marjoh3 <@t> telus.net  Thu Sep 25 16:09:52 2014
From: marjoh3 <@t> telus.net (Marilyn Johnson)
Date: Thu Sep 25 16:09:50 2014
Subject: [Histonet] Manual For Reichert Jung Autocut 1150
Message-ID: <AC945B90569E4C27AA35235C26F33158@OwnerPC>


Hi Histonetters,

I am looking for a manual for a Reichert Jung Autocut microtome 1150 with a stereo zoom optic system.

If anyone can provide me with this manual or a source of obtaining one would be greatly appreciated. 

Thank you in advance.

Marilyn Johnson

Apex Histology Labs.
From akelley <@t> path.wustl.edu  Thu Sep 25 17:25:00 2014
From: akelley <@t> path.wustl.edu (Kelley, Amanda)
Date: Thu Sep 25 17:25:12 2014
Subject: [Histonet] MHT November  and CellMarque Lunch N Learn
Message-ID: <F053AFDDBC94524998697F264BEC3EB31974FD24@pathmbx02.wusm-path.wustl.edu>

The Missouri Society for Histotechnology (MHT) is having a Fall Lunch n Learn sponsored by CellMarque.

CELL MARQUE AND MHT
***Present a Fall Lunch n' Learn***
Basic IHC and Troubleshooting
Workshop designed to improve basic IHC knowledge and uncover what goes on "behind the scenes" inside your IHC
automated platform. It will touch on different types of detection, explaining the advantages and disadvantages of each
along with how to determine the best one for your assay. Common causes of suboptimal staining and troubleshooting
will be discussed. The session will open up to participants' troubleshooting questions for group discussion. (3 CEU)

Cancer and Antibody Challenge
Get a glimpse at how IHC comes into play for a patient. This workshop will be a fun, interactive look at how IHC are
used by pathologists. Discussion will be geared for an audience with a basic to intermediate level of IHC understanding.
Types of cancers and statistics will be discussed along with frequently used panels and their interpretation. We will touch
briefly on some of the more advanced algorithms as well. We will present real life scenarios accompanied with various
clues and staining results, and allow the audience to use their newly acquired knowledge of panel usage to decide what
markers might be helpful. At the conclusion of this talk, the audience will have seen for themselves how important IHC
can be, and also how panels can be a great tool to assist pathologists with finding an accurate diagnosis. (3 CEU)

Presenter: Matt Pardilla, Technical Consultant, Cell Marque Corporation
When: Thursday, November 6, 2014
Where: St. Luke's Hospital
232 South Woods Mill Road
Chesterfield, MO 63017
Institute Conference Room 1-3
Time: 8:30 am - 4:00 pm (Lunch will be provided)
Cost: Free for MHT Members, $25.00 for Non MHT Members (Join the MHT for $15.00 for free Lunch n' Learn CEU's,
reduced symposium fees and great networking). For information call Sharon Walsh at 314-941-2301 or
president.mht@missourihisto.org. Information available on Face book and www.missourihisto.org





The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
From Lyndsey.Buechel <@t> covance.com  Fri Sep 26 10:05:33 2014
From: Lyndsey.Buechel <@t> covance.com (Buechel, Lyndsey)
Date: Fri Sep 26 10:05:48 2014
Subject: [Histonet] Supervisors Needed
Message-ID: <F624D839BEA2654B94161AFF87E724DA2B11A6B5@DFWPW0EXCH010.ent.covance.com>

Hi Histonetters. Wanted to share that Covance is hiring Supervisors to oversee a group of histology and necropsy technicians. We have open positions both in Madison, WI and Greenfield, IN.

You can view the job descriptions and apply here.

For the Madison location: http://jobsearch.covance.com/jobs/1030949-Lab-Supervisor-Madison-WI.aspx
For the Greenfield location: http://jobsearch.covance.com/jobs/1007602-Histology-Supervisor.aspx

Feel free to contact me directly if you have any questions.

Lyndsey Buechel
Recruiter
Recruitment and Talent Advisors
Covance Inc.
Minneapolis, MN
Tel:  +1 218 297 4125
E-mail:  lyndsey.buechel@covance.com<mailto:lyndsey.buechel@covance.com>
Apply online at: www.covancecareers.com<http://www.covancecareers.com/>
________________________________
Confidentiality Notice: In accordance with Covance's Data Classification Policy, this email, including attachment(s), is classified as Confidential or Highly Confidential. This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or dissemination of the content of this e-mail is strictly prohibited.

If you have received this e-mail transmission in error or this email is not intended for you, please delete or destroy all copies of this message in your possession and inform the sender. Thank you.
From jaylundgren <@t> gmail.com  Fri Sep 26 10:07:53 2014
From: jaylundgren <@t> gmail.com (Jay Lundgren)
Date: Fri Sep 26 10:08:03 2014
Subject: [Histonet] Dragon Voice Recognition software
In-Reply-To: <E5D0CD2352E46545A0C0EBE308CCCE53342384F1@L1PERDWXMB02.childrensroot.net>
References: <E5D0CD2352E46545A0C0EBE308CCCE53342384F1@L1PERDWXMB02.childrensroot.net>
Message-ID: <CANCZNuYwDgBpSWgrDXPfOH2fzULrEj1okiJ0-LjbTNXbmkN=kQ@mail.gmail.com>

I'd imagine it would really upset the transcriptionists, because you
wouldn't need them anymore?

On Thu, Sep 25, 2014 at 8:06 AM, Houston, Ronald <
Ronald.Houston@nationwidechildrens.org> wrote:

> Is anyone using this transcribing directly into Sunquest CoPathPlus?
>
> How successful was the transition, what problems did you encounter, and
> what resistance if any was there from pathologists, PAs and
> transcriptionists?
>
> Thanks
>
> Ronnie Houston, MS HT(ASCP)QIHC FIBMS
> Anatomic Pathology Manager
> ChildLab, a Division of Nationwide Children's Hospital
> www.childlab.com
>
> 700 Children's Drive
> Columbus, OH 43205
> (P) 614-722-5450
> (F) 614-722-2899
> ronald.houston@nationwidechildrens.org<mailto:
> ronald.houston@nationwidechildrens.org>
> www.NationwideChildrens.org<http://www.nationwidechildrens.org/>
>
> "One person with passion is better than forty people merely interested."
> ~ E.M. Forster
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From Timothy.Morken <@t> ucsfmedctr.org  Fri Sep 26 11:08:14 2014
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy)
Date: Fri Sep 26 11:08:24 2014
Subject: [Histonet] TGIF
Message-ID: <761E2B5697F795489C8710BCC72141FF3679A6A9@ex07.net.ucsf.edu>

Some fun for FRIDAY!

http://histosearch.com/imageupload/what-my-friends-think-i-do-histotech/



Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.morken@ucsfmedctr.org<mailto:tim.morken@ucsfmedctr.org>

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.



From Ronald.Houston <@t> nationwidechildrens.org  Fri Sep 26 11:33:02 2014
From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald)
Date: Fri Sep 26 11:33:14 2014
Subject: [Histonet] Dragon Voice Recognition software
In-Reply-To: <CANCZNuYwDgBpSWgrDXPfOH2fzULrEj1okiJ0-LjbTNXbmkN=kQ@mail.gmail.com>
References: <E5D0CD2352E46545A0C0EBE308CCCE53342384F1@L1PERDWXMB02.childrensroot.net>
	<CANCZNuYwDgBpSWgrDXPfOH2fzULrEj1okiJ0-LjbTNXbmkN=kQ@mail.gmail.com>
Message-ID: <E5D0CD2352E46545A0C0EBE308CCCE533423993F@L1PERDWXMB02.childrensroot.net>

we've got plenty of other tasks for them to do! Plus we are an academic center so there are grants to write, articles, abstratcs and presentations to prepare, send-outs and consults to handle?.the list goes on and on

From: Jay Lundgren [mailto:jaylundgren@gmail.com]
Sent: Friday, September 26, 2014 11:08 AM
To: Houston, Ronald
Cc: Histonet
Subject: Re: [Histonet] Dragon Voice Recognition software

I'd imagine it would really upset the transcriptionists, because you wouldn't need them anymore?

On Thu, Sep 25, 2014 at 8:06 AM, Houston, Ronald <Ronald.Houston@nationwidechildrens.org<mailto:Ronald.Houston@nationwidechildrens.org>> wrote:
Is anyone using this transcribing directly into Sunquest CoPathPlus?

How successful was the transition, what problems did you encounter, and what resistance if any was there from pathologists, PAs and transcriptionists?

Thanks

Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com<http://www.childlab.com>

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450<tel:614-722-5450>
(F) 614-722-2899<tel:614-722-2899>
ronald.houston@nationwidechildrens.org<mailto:ronald.houston@nationwidechildrens.org><mailto:ronald.houston@nationwidechildrens.org<mailto:ronald.houston@nationwidechildrens.org>>
www.NationwideChildrens.org<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.NationwideChildrens.org&d=AAMFaQ&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=aQFcUd_DjQa8A0Yvg9APpI-A9G-O0NgHeHwbMB-7Vm8&s=riZM3u90f-Tnv1Vewg5ssn0Fjq9CWTvhFSJUk0fD32Q&e=><http://www.nationwidechildrens.org/>

"One person with passion is better than forty people merely interested."
~ E.M. Forster


_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet<https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AAMFaQ&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=aQFcUd_DjQa8A0Yvg9APpI-A9G-O0NgHeHwbMB-7Vm8&s=z5QLFututFQACPR5IlCB1zRk1ael9lB7jOAoAr5BtyE&e=>

From Ronald.Houston <@t> nationwidechildrens.org  Fri Sep 26 11:40:59 2014
From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald)
Date: Fri Sep 26 11:41:08 2014
Subject: [Histonet] IF on paraffin
Message-ID: <E5D0CD2352E46545A0C0EBE308CCCE5334239987@L1PERDWXMB02.childrensroot.net>

Anyone doing this? Needs some pointers in balancing quenching autofluorescence and suppression of fluorescent signal

Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston@nationwidechildrens.org<mailto:ronald.houston@nationwidechildrens.org>
www.NationwideChildrens.org<http://www.nationwidechildrens.org/>

"One person with passion is better than forty people merely interested."
~ E.M. Forster

From sameena.k.masood <@t> Vanderbilt.Edu  Fri Sep 26 11:52:23 2014
From: sameena.k.masood <@t> Vanderbilt.Edu (Masood, S. Kathryn)
Date: Fri Sep 26 11:53:07 2014
Subject: [Histonet] can you unsubscribe me please?
Message-ID: <AED6644A7A94A74D964DADB50A378CDE484EB86F@ITS-HCWNEM101.ds.vanderbilt.edu>



Kathryn

S. Kathryn Masood
Vanderbilt University School of Nursing
405 Godchaux Hall
461 21st Avenue South
Nashville, TN  37240
Office #:  615-936-1719
Email:  sameena.k.masood@vanderbilt.edu<mailto:sameena.k.masood@vanderbilt.edu>

From Joyce.Weems <@t> emoryhealthcare.org  Fri Sep 26 11:56:36 2014
From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.)
Date: Fri Sep 26 11:56:48 2014
Subject: [Histonet] RE: can you unsubscribe me please?
In-Reply-To: <AED6644A7A94A74D964DADB50A378CDE484EB86F@ITS-HCWNEM101.ds.vanderbilt.edu>
References: <AED6644A7A94A74D964DADB50A378CDE484EB86F@ITS-HCWNEM101.ds.vanderbilt.edu>
Message-ID: <E3A4EBD57A691646BCCED4AA5911A030011C3513F6@e14mbx12n.Enterprise.emory.net>

Hi Kathryn,

You will need to do this at http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Best wishes!

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s).  It may contain information that is privileged and confidential.  Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Masood, S. Kathryn
Sent: Friday, September 26, 2014 12:52 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] can you unsubscribe me please?



Kathryn

S. Kathryn Masood
Vanderbilt University School of Nursing
405 Godchaux Hall
461 21st Avenue South
Nashville, TN  37240
Office #:  615-936-1719
Email:  sameena.k.masood@vanderbilt.edu<mailto:sameena.k.masood@vanderbilt.edu>

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

________________________________

This e-mail message (including any attachments) is for the sole use of
the intended recipient(s) and may contain confidential and privileged
information. If the reader of this message is not the intended
recipient, you are hereby notified that any dissemination, distribution
or copying of this message (including any attachments) is strictly
prohibited.

If you have received this message in error, please contact
the sender by reply e-mail message and destroy all copies of the
original message (including attachments).

From lins0701 <@t> gmail.com  Fri Sep 26 12:06:15 2014
From: lins0701 <@t> gmail.com (Sophia Lin)
Date: Fri Sep 26 12:06:23 2014
Subject: [Histonet] Histotech Wanted
Message-ID: <CAJGPzdTpbchO3T2ghDu+fCQPR2Ji6zG8251LzURnf+gsxRjxGw@mail.gmail.com>

We are a small GI laboratory looking for a part time histotechnician.
Certification suggested, but not required. Looking for dedicated and
committed person who is a team player and can work well with others.

Please submit applications to lins0701@gmail.com

Thanks!
From JWatson <@t> gnf.org  Fri Sep 26 12:08:43 2014
From: JWatson <@t> gnf.org (James Watson)
Date: Fri Sep 26 12:08:48 2014
Subject: [Histonet] RE: IF on paraffin
In-Reply-To: <E5D0CD2352E46545A0C0EBE308CCCE5334239987@L1PERDWXMB02.childrensroot.net>
References: <E5D0CD2352E46545A0C0EBE308CCCE5334239987@L1PERDWXMB02.childrensroot.net>
Message-ID: <A032B25305EC6949AC9AFF23C9620616CA260412@EX5.lj.gnf.org>

Ronald,

We stain IF on paraffin all the time, typically about 100-200 slides per week .  We quench all of our fluorescence work with CuSO4.  Here is our CuSo4 quenching technique.  Since quenching depends on what is causing the autofluorescence we have other protocols using 2.0mM Glycine, Sodium Borohydride, and Sudan Black.  We use them rarely.

10 mM Copper Sulfate
	i)	This treatment is primarily for inhibition of autofluorescence in Red Blood cells, but helps decrease autofluorescence in 		connective tissue.
	ii)	10 mM Copper Sulfate
		(1)	Cupric Sulfate...............1.25 gm.
		(2)	50 mM Ammonium acetate (pH5)........500.0 ml
		(3)	Adjust pH to 5.0 with 1.0 M NaOH
	iii)	50 mM Ammonium acetate (pH5)
		(1)	Ammonium acetate.............1.93 gm.
		(2)	Distilled water................500.0 ml
		(3)	Adjust pH to 5.0 with 1.0 M HCl
	iv)	Treatment Procedure
		(1)	Rinse in PBS 2 times for 10 minutes each.
		(2)	Rinse in distilled water 5 minutes.
		(3)	Place slides in 10mM copper sulfate for 8 minutes.
		(4)	Return slides to distilled water and check for autofluorescence with microscope.
		(5)	If needed return slides to 10mM copper sulfate for a couple of more minutes and check again.
		(6)	Rinse slides for 5 minutes in distilled water.
		(7)	Rinse in PBS 2 times for 10 minutes each.
		(8)	Coverslip slides with appropriate mounting media.

James Watson HT? ASCP
GNF? Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel??? 858-332-4647
Fax?? 858-812-1915
jwatson@gnf.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald
Sent: Friday, September 26, 2014 9:41 AM
To: Histonet
Subject: [Histonet] IF on paraffin

Anyone doing this? Needs some pointers in balancing quenching autofluorescence and suppression of fluorescent signal

Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston@nationwidechildrens.org<mailto:ronald.houston@nationwidechildrens.org>
www.NationwideChildrens.org<http://www.nationwidechildrens.org/>

"One person with passion is better than forty people merely interested."
~ E.M. Forster

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From rooster3801 <@t> yahoo.com  Fri Sep 26 12:13:04 2014
From: rooster3801 <@t> yahoo.com (Rooster)
Date: Fri Sep 26 12:13:13 2014
Subject: [Histonet] MediaLab is Looking for Histology Course Authors
In-Reply-To: <SNT149-W724F3A2B3498DC5381D20CD8BE0@phx.gbl>
References: <CAC2QUZ_+Ky6FNGx2ptU9Wopjp4NX7=g-J2N+SLNT2ubS325s=A@mail.gmail.com>
	<SNT149-W724F3A2B3498DC5381D20CD8BE0@phx.gbl>
Message-ID: <D722567D-8979-466C-8D01-903537448776@yahoo.com>

I would be very interested in working in any teaching capacity for histology. Please contact me further and tell me how I can get involved. 

Thanx, 

Christopher M. Copeland HT(ASCP)




> On Sep 25, 2014, at 3:33 PM, Joelle Weaver <joelleweaver@hotmail.com> wrote:
> 
> I wanted to say that when I have worked with Medilab on histology related courses in the past that it has been a great experience for me. I highly recommend it as a great learning opportunity for anyone who is interested in working with education, development and training in their practice area.  I just wanted to give some first hand feedback, and encourage anyone who may have the interest or inclination to consider becoming an author or reviewer.  
> 
> 
> Joelle Weaver MAOM, HTL (ASCP) QIHC
> 
> 
> 
> 
> 
>> Date: Thu, 25 Sep 2014 14:30:16 -0400
>> From: judi@medialabinc.net
>> To: histonet@lists.utsouthwestern.edu
>> Subject: [Histonet] MediaLab is Looking for Histology Course Authors
>> 
>> Actively seeking authors to write and review online *histology courses* for
>> *MediaLab*!  MediaLab is a leading publisher of online continuing education
>> (CE) courses and competency assessments. Our online products are used at
>> more than 2,000 laboratories and university CLS/HT/HTL programs worldwide.
>> 
>> This is a great opportunity to *gain resume-boosting publishing
>> experience, **earn honorariums* for your participation, and fill the *need to
>> provide quality histology CE credits*.
>> 
>> Authors can take advantage of MediaLab's online CourseBuilder to *write
>> courses anytime, anywhere*. CourseBuilder is easy to use, with an intuitive
>> interface similar to Microsoft Word or PowerPoint. Authors can quickly
>> create content pages, practice questions, and exam questions, and upload
>> relevant images.
>> 
>> Courses developed by MediaLab are *featured on our websites MediaLabInc.net
>> and LabCE.com*. Questions from these courses also become part of the
>> LabCE.com Quiz Game, with over 1,000 daily players. Authors and reviewers
>> may also*contribute to other online programs that we develop on behalf of
>> major laboratory partners*.
>> 
>> To learn more about becoming a MediaLab author for histology courses, visit
>> our online information page atwww.medialbinc.net/authors.aspx .  Please
>> contact Judi Bennett at judi@medialabinc.net  or call 877-776-8460, ext. 721
>> .
>> Thank you,
>> Judi
>> 
>> 
>> Judi Bennett, MT, BSM
>> 
>> Program Director - MediaLab, Inc.
>> e-mail judi@medialabinc.net
>> Phone (877) 776-8460 ext. 721
>> 
>> cell phone 404-915-2999
>> fax (678) 401-0284
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>                         _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From lins0701 <@t> gmail.com  Fri Sep 26 12:24:28 2014
From: lins0701 <@t> gmail.com (Sophia Lin)
Date: Fri Sep 26 12:24:34 2014
Subject: [Histonet] (no subject)
Message-ID: <CAJGPzdRf8BJ3zO26OdpEaE3eS5fCZL0kO3BdXT1XDWo4jEXiUA@mail.gmail.com>

We are a small GI laboratory in Murrieta, CA looking for a part time
histotechnician. Certification suggested, but not required. Looking for
dedicated and committed person who is a team player and can work well with
others.

Please submit applications to lins0701@gmail.com

Thanks!
From skiousis <@t> med.wayne.edu  Fri Sep 26 12:33:41 2014
From: skiousis <@t> med.wayne.edu (Kiousis, Sam)
Date: Fri Sep 26 12:33:49 2014
Subject: [Histonet] double-staining / multi-color / co-localization IHC
Message-ID: <18E6F3EDA42AB04280E9C218D20832361C755754@MED-CORE07B.med.wayne.edu>

Does anyone have any experience with double-staining / multi-color / co-localization immunohistochemistry?  Any protocols or kits you have used in the past would be most helpful.  I'm attempting to use 2 antibodies, CD68 and HER2, that have worked well in the past individually and combine them together on PFA-fixed, OCT frozen blocks of human brain tumor specimens.

Thanks in advance,

Sam Kiousis
Neuro-Oncology
Karmanos Cancer Institute
skiousis@med.wayne.edu<mailto:skiousis@med.wayne.edu>


________________________________

This document may include proprietary and confidential information. This document may not be reproduced, copied, distributed, published, modified or furnished to third parties, without prior written consent. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. If you are not the intended recipient you are notified that disclosing, copying, distributing or taking any action in reliance on the contents of this information is strictly prohibited.
From mike <@t> dbiosys.com  Fri Sep 26 12:38:40 2014
From: mike <@t> dbiosys.com (Michael Thompson)
Date: Fri Sep 26 12:41:55 2014
Subject: [Histonet] double-staining / multi-color / co-localization IHC
In-Reply-To: <18E6F3EDA42AB04280E9C218D20832361C755754@MED-CORE07B.med.wayne.edu>
References: <18E6F3EDA42AB04280E9C218D20832361C755754@MED-CORE07B.med.wayne.edu>
Message-ID: <p314y0myyfchm3hxhiw5mw9h.1411753159977@email.android.com>

Please see our website.  We have 6 different colors and all kits needed.  I sm happy to help.



Michael Thompson
Dir of Sales
Diagnostic BioSystems
4128601288
www.dbiosys.com<http://www.dbiosys.com>
"IHC Made Affordable"


Sent from my Verizon Wireless 4G LTE DROID


"Kiousis, Sam" <skiousis@med.wayne.edu> wrote:

Does anyone have any experience with double-staining / multi-color / co-localization immunohistochemistry?  Any protocols or kits you have used in the past would be most helpful.  I'm attempting to use 2 antibodies, CD68 and HER2, that have worked well in the past individually and combine them together on PFA-fixed, OCT frozen blocks of human brain tumor specimens.

Thanks in advance,

Sam Kiousis
Neuro-Oncology
Karmanos Cancer Institute
skiousis@med.wayne.edu<mailto:skiousis@med.wayne.edu>


________________________________

This document may include proprietary and confidential information. This document may not be reproduced, copied, distributed, published, modified or furnished to third parties, without prior written consent. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. If you are not the intended recipient you are notified that disclosing, copying, distributing or taking any action in reliance on the contents of this information is strictly prohibited.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From plucas <@t> biopath.org  Fri Sep 26 15:00:41 2014
From: plucas <@t> biopath.org (Paula Lucas)
Date: Fri Sep 26 14:54:10 2014
Subject: [Histonet] (no subject)
Message-ID: <0AE457C6EFEE49E8A4B2F158DC44AE97@biopath.local>

Hello,

We are doing a study on molar roots for a company in Orange County,
California. The study is working with tissues embedded in paraffin blocks
and they would like a study using resin embedded histology.  We do not have
the capability to do this study, so if your company is able to help them,
and would like to contact them to find out more information, please let me
know and I will connect you to the right person.  

Thank you,

Paula Lucas

Lab Manager

Bio-Path Medical Group

From plucas <@t> biopath.org  Fri Sep 26 15:01:22 2014
From: plucas <@t> biopath.org (Paula Lucas)
Date: Fri Sep 26 14:54:49 2014
Subject: [Histonet] Study using Resin Embedding 
Message-ID: <CB2CAC9B5A9F429C94166602A8A42CF0@biopath.local>

Hello,

We are doing a study on molar roots for a company in Orange County,
California. The study is working with tissues embedded in paraffin blocks
and they would like a study using resin embedded histology.  We do not have
the capability to do this study, so if your company is able to help them,
and would like to contact them to find out more information, please let me
know and I will connect you to the right person.  

Thank you,

Paula Lucas

Lab Manager

Bio-Path Medical Group

From joelleweaver <@t> hotmail.com  Fri Sep 26 15:24:35 2014
From: joelleweaver <@t> hotmail.com (Joelle Weaver)
Date: Fri Sep 26 15:24:39 2014
Subject: [Histonet] TGIF
In-Reply-To: <761E2B5697F795489C8710BCC72141FF3679A6A9@ex07.net.ucsf.edu>
References: <761E2B5697F795489C8710BCC72141FF3679A6A9@ex07.net.ucsf.edu>
Message-ID: <SNT149-W40995F6973E98FC289F993D8BF0@phx.gbl>

This is super funny, Tim. My personal favorite is on occasion HR people have mispronounced as offered me a "history" position. Not sure what that would me I would do? 


Joelle Weaver MAOM, HTL (ASCP) QIHC

        
  

 
> From: Timothy.Morken@ucsfmedctr.org
> To: histonet@lists.utsouthwestern.edu
> Date: Fri, 26 Sep 2014 16:08:14 +0000
> Subject: [Histonet] TGIF
> 
> Some fun for FRIDAY!
> 
> http://histosearch.com/imageupload/what-my-friends-think-i-do-histotech/
> 
> 
> 
> Tim Morken
> Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
> UC San Francisco Medical Center
> Box 1656
> 505 Parnassus Ave
> San Francisco, CA 94143
> USA
> 
> 415.514-6042  (office)
> tim.morken@ucsfmedctr.org<mailto:tim.morken@ucsfmedctr.org>
> 
> CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From joelleweaver <@t> hotmail.com  Fri Sep 26 15:40:51 2014
From: joelleweaver <@t> hotmail.com (Joelle Weaver)
Date: Fri Sep 26 15:40:58 2014
Subject: [Histonet] TGIF
In-Reply-To: <SNT149-W40995F6973E98FC289F993D8BF0@phx.gbl>
References: <761E2B5697F795489C8710BCC72141FF3679A6A9@ex07.net.ucsf.edu>,
	<SNT149-W40995F6973E98FC289F993D8BF0@phx.gbl>
Message-ID: <SNT149-W2403909DF0758119B00E1AD8BF0@phx.gbl>

Wow, I have been up too long, sorry for the typos...I hope you all can get the point and joke. Happy weekend!


Joelle Weaver MAOM, HTL (ASCP) QIHC

        
  

 
> From: joelleweaver@hotmail.com
> To: timothy.morken@ucsfmedctr.org; histonet@lists.utsouthwestern.edu
> Date: Fri, 26 Sep 2014 20:24:35 +0000
> Subject: RE: [Histonet] TGIF
> CC: 
> 
> This is super funny, Tim. My personal favorite is on occasion HR people have mispronounced as offered me a "history" position. Not sure what that would me I would do? 
> 
> 
> Joelle Weaver MAOM, HTL (ASCP) QIHC
> 
>         
>   
> 
>  
> > From: Timothy.Morken@ucsfmedctr.org
> > To: histonet@lists.utsouthwestern.edu
> > Date: Fri, 26 Sep 2014 16:08:14 +0000
> > Subject: [Histonet] TGIF
> > 
> > Some fun for FRIDAY!
> > 
> > http://histosearch.com/imageupload/what-my-friends-think-i-do-histotech/
> > 
> > 
> > 
> > Tim Morken
> > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
> > UC San Francisco Medical Center
> > Box 1656
> > 505 Parnassus Ave
> > San Francisco, CA 94143
> > USA
> > 
> > 415.514-6042  (office)
> > tim.morken@ucsfmedctr.org<mailto:tim.morken@ucsfmedctr.org>
> > 
> > CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.
> > 
> > 
> > 
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>  		 	   		  _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From joelleweaver <@t> hotmail.com  Fri Sep 26 15:59:03 2014
From: joelleweaver <@t> hotmail.com (Joelle Weaver)
Date: Fri Sep 26 15:59:07 2014
Subject: [Histonet] Dragon Voice Recognition software
In-Reply-To: <CANCZNuYwDgBpSWgrDXPfOH2fzULrEj1okiJ0-LjbTNXbmkN=kQ@mail.gmail.com>
References: <E5D0CD2352E46545A0C0EBE308CCCE53342384F1@L1PERDWXMB02.childrensroot.net>,
	<CANCZNuYwDgBpSWgrDXPfOH2fzULrEj1okiJ0-LjbTNXbmkN=kQ@mail.gmail.com>
Message-ID: <SNT149-W234C74D9EB71833732837D8BF0@phx.gbl>

The work that can be done in histology-pathology is endless! I know that in past positions that sometimes transcriptionists have felt threatened by voice recognition dictation and software when it was suggested. I don't really blame them for these feelings, but it never turned out the way they worried it might. 
 
Machines or computer software never completely replace people, they don't "think", make decisions or have judgment. The people just have to function at a higher level or in an alternate way since the rudimentary processes for the people are reduced .  
 
I just set up Dragon dictation for the microscopic for the pathologists, but it will be in Orchard Pathology, so could not help with the interface to your system Ronnie. But it did not replace the information management function or its oversight, just changed the tasks that needed to be performed somewhat, and increased overall efficiency. 


Joelle Weaver MAOM, HTL (ASCP) QIHC

        
  

 
> Date: Fri, 26 Sep 2014 10:07:53 -0500
> From: jaylundgren@gmail.com
> To: Ronald.Houston@nationwidechildrens.org
> Subject: Re: [Histonet] Dragon Voice Recognition software
> CC: histonet@lists.utsouthwestern.edu
> 
> I'd imagine it would really upset the transcriptionists, because you
> wouldn't need them anymore?
> 
> On Thu, Sep 25, 2014 at 8:06 AM, Houston, Ronald <
> Ronald.Houston@nationwidechildrens.org> wrote:
> 
> > Is anyone using this transcribing directly into Sunquest CoPathPlus?
> >
> > How successful was the transition, what problems did you encounter, and
> > what resistance if any was there from pathologists, PAs and
> > transcriptionists?
> >
> > Thanks
> >
> > Ronnie Houston, MS HT(ASCP)QIHC FIBMS
> > Anatomic Pathology Manager
> > ChildLab, a Division of Nationwide Children's Hospital
> > www.childlab.com
> >
> > 700 Children's Drive
> > Columbus, OH 43205
> > (P) 614-722-5450
> > (F) 614-722-2899
> > ronald.houston@nationwidechildrens.org<mailto:
> > ronald.houston@nationwidechildrens.org>
> > www.NationwideChildrens.org<http://www.nationwidechildrens.org/>
> >
> > "One person with passion is better than forty people merely interested."
> > ~ E.M. Forster
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
From GKeyser <@t> uwhealth.org  Fri Sep 26 16:23:31 2014
From: GKeyser <@t> uwhealth.org (Keyser Gerald  T)
Date: Fri Sep 26 16:23:38 2014
Subject: [Histonet] Dragon Voice Recognition software
In-Reply-To: <SNT149-W234C74D9EB71833732837D8BF0@phx.gbl>
References: <E5D0CD2352E46545A0C0EBE308CCCE53342384F1@L1PERDWXMB02.childrensroot.net>,
	<CANCZNuYwDgBpSWgrDXPfOH2fzULrEj1okiJ0-LjbTNXbmkN=kQ@mail.gmail.com>
	<SNT149-W234C74D9EB71833732837D8BF0@phx.gbl>
Message-ID: <5226C88C65EBFF4BAD552D68DC6E8FFE038409@UWHC-MBX12.uwhis.hosp.wisc.edu>

Voice to text cannot cope with doctors mumbling into recorders. Even the best VRS only hits about 95% of the regular dictionary; which is very good. But, a 5% error rate is wildly unacceptable in a clinical environment.  

Another parallel is trying to apply handwriting recognition software to a physician penmanship. Would totally make the software divide by zero and have a fit. 

gerry

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joelle Weaver
Sent: Friday, September 26, 2014 3:59 PM
To: Jay Lundgren; Houston, Ronald
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Dragon Voice Recognition software

The work that can be done in histology-pathology is endless! I know that in past positions that sometimes transcriptionists have felt threatened by voice recognition dictation and software when it was suggested. I don't really blame them for these feelings, but it never turned out the way they worried it might. 
 
Machines or computer software never completely replace people, they don't "think", make decisions or have judgment. The people just have to function at a higher level or in an alternate way since the rudimentary processes for the people are reduced .  
 
I just set up Dragon dictation for the microscopic for the pathologists, but it will be in Orchard Pathology, so could not help with the interface to your system Ronnie. But it did not replace the information management function or its oversight, just changed the tasks that needed to be performed somewhat, and increased overall efficiency. 


Joelle Weaver MAOM, HTL (ASCP) QIHC

        
  

 
> Date: Fri, 26 Sep 2014 10:07:53 -0500
> From: jaylundgren@gmail.com
> To: Ronald.Houston@nationwidechildrens.org
> Subject: Re: [Histonet] Dragon Voice Recognition software
> CC: histonet@lists.utsouthwestern.edu
> 
> I'd imagine it would really upset the transcriptionists, because you 
> wouldn't need them anymore?
> 
> On Thu, Sep 25, 2014 at 8:06 AM, Houston, Ronald < 
> Ronald.Houston@nationwidechildrens.org> wrote:
> 
> > Is anyone using this transcribing directly into Sunquest CoPathPlus?
> >
> > How successful was the transition, what problems did you encounter, 
> > and what resistance if any was there from pathologists, PAs and 
> > transcriptionists?
> >
> > Thanks
> >
> > Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager 
> > ChildLab, a Division of Nationwide Children's Hospital 
> > www.childlab.com
> >
> > 700 Children's Drive
> > Columbus, OH 43205
> > (P) 614-722-5450
> > (F) 614-722-2899
> > ronald.houston@nationwidechildrens.org<mailto:
> > ronald.houston@nationwidechildrens.org>
> > www.NationwideChildrens.org<http://www.nationwidechildrens.org/>
> >
> > "One person with passion is better than forty people merely interested."
> > ~ E.M. Forster
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  _______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From KSimeone <@t> leavittmgt.com  Mon Sep 29 07:07:32 2014
From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone)
Date: Mon Sep 29 07:07:40 2014
Subject: [Histonet] FT NIGHT POSTION TECHNOLOGIST DELRAY, FL
Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C2FDA376B@vm-email.leavittmgt.com>

Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE.



***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!



Email your resume to lengimann@leavittmgt.com<mailto:lengimann@leavittmgt.com> if interested.



*full time position Mon-Fri PM (will discuss hours upon interview/will be AFTER 5p)

*MUST be licensed as a FL HISTOTECHNOLOGIST

*MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE!

*VERY proficient in embedding and microtomy

*must be self motivated, reliable and a team player

*knowledge in operating Ventana and Leica equipment desired (not necessary)



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

Delray Beach Technical Laboratory

ADCS Clinics, LLC

Advanced Dermatology and Cosmetic Surgery

Ameriderm

561.819.0857 ext 100

561.819.6517 fax

ksimeone@leavittmgt.com<mailto:ksimeone@leavittmgt.com>

www.advancedderm.com<http://www.advancedderm.com/>



The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message.


From mw <@t> personifysearch.com  Mon Sep 29 10:12:53 2014
From: mw <@t> personifysearch.com (Matt Ward)
Date: Mon Sep 29 10:12:59 2014
Subject: [Histonet] Field Based Histology Opportunity 
Message-ID: <070f01cfdbf7$d8031740$880945c0$@personifysearch.com>

Good morning!

 

My team and I specialize in recruiting for  the Histology/AP space.
Recently, we have had a Field Applications/Support opportunity with a
retained client come available in the Northeast market. The position offers
a great package and a terrific career path. 

 

We would like to connect with you to further discuss the details and see if
you are interested/qualified or if you know of a friend or colleague who may
be open to networking. 

 

The company is a world leader in Cancer Diagnostics and part of a $19B
organization!!

Great Opportunity to grow with the company!!

Opportunity to break out of the lab and into industry!!

  

Some of the specifications of this position are as follows:

  

Title: Field Support Specialist - Northeast 

 

Description: Provides training and support to histology product line. 

Product: Histology Products 

Territory: New England/Northeast 

Company Car: Yes

 
**Requires a 4 year degree and valid driver's license**

 

If this sounds of interest to you, please send me your latest resume and
let's catch up.   If you are not looking at this particular time, I would
still like to connect with you. Because of our focus in the diagnostic
market, I believe we will be able to find you a position of interest in the
future. 

 

Please respond to me directly at mw@personifysearch.com
<mailto:mw@personifysearch.com>  

 

I look forward to working with you!

 

Thanks! 

 

Matt Ward

Program Manager 

Personify

5020 Weston Parkway Suite 315

Cary NC 27513 

(Tel) 919.459.3654

(Tel) 800.875.6188 direct ext 103

(Fax) 919.882.8727

  <http://www.personifysearch.com/> www.personifysearch.com

 

From mthomas <@t> littonlab.com  Mon Sep 29 11:22:13 2014
From: mthomas <@t> littonlab.com (Marla Thomas)
Date: Mon Sep 29 11:22:23 2014
Subject: [Histonet] job opening
Message-ID: <fa347d5a87ab43859775a5ed8afd8c07@BLUPR07MB834.namprd07.prod.outlook.com>

We have a job opening for a Full-time HT(ASCP), Mon-Fri, day shift (early hours).  EXPERIENCE A MUST.  No Headhunters.

Please send resumes to: mthomas@littonlab.com

Marla Thomas, HT(ASCP)
Business Manager
Litton Pathology Associates, PC
700 NW Hunter Dr.
Blue Springs, MO 64015
Phone: 816-229-6449 Fax:816-874-4400

CONFIDENTIALITY NOTICE
This message and any included attachments are from Litton Pathology Associates, P.C. and are intended only for the addressee.  The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.  Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.  If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 816-229-6449 and ask for the HIPAA/Compliance Coordinator.

From Timothy.Morken <@t> ucsfmedctr.org  Mon Sep 29 11:24:59 2014
From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy)
Date: Mon Sep 29 11:26:12 2014
Subject: [Histonet] Any labs able to do whole cross section of human larynx
 for research?
Message-ID: <761E2B5697F795489C8710BCC72141FF3679AA15@ex07.net.ucsf.edu>

I have a question from another institution about doing whole mount cross section of a human larynx for a research project. Can anyone handle that?

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.morken@ucsfmedctr.org<mailto:tim.morken@ucsfmedctr.org>

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.



From kaarrington <@t> anthc.org  Mon Sep 29 13:24:45 2014
From: kaarrington <@t> anthc.org (Arrington, Karla A)
Date: Mon Sep 29 13:24:55 2014
Subject: [Histonet] Negative Control for Validation
Message-ID: <F362122314DD5C41BD407A39C4FCF8757B9C1E02@AKHV-EX2K12MB3.alaska.ihs.gov>

We are beginning the process of validating new antibodies using the BenchMark XT but have some questions for validation.

Question:  For a positive reagent control that stains appropriately positive, is a known control block used? If not, what is used?
Question:  For a negative reagent control that stains appropriately negative, what is ran?
Question:  Is a negative tissue slide ran for all of our 10 cases per antibody, or per case that will be used for validation?

Thanks All!
Karla

Karla Arrington, HT(ASCP), HIT(AHIMA)
Lead Histology Technician
ANMC Pathology
907-729-1810
kaarrington@anthc.org

From suetp918 <@t> comcast.net  Mon Sep 29 15:29:59 2014
From: suetp918 <@t> comcast.net (Sue)
Date: Mon Sep 29 15:30:21 2014
Subject: [Histonet] Negative Control for Validation
In-Reply-To: <F362122314DD5C41BD407A39C4FCF8757B9C1E02@AKHV-EX2K12MB3.alaska.ihs.gov>
References: <F362122314DD5C41BD407A39C4FCF8757B9C1E02@AKHV-EX2K12MB3.alaska.ihs.gov>
Message-ID: <247860894.5392641.1412022598998.JavaMail.root@comcast.net>

What we do here is run a mix of expected negative and positive cases this way we are sure we are not getting exogenous staining.? Depending on the antibody we try to run at least a mix of 25 cases.? In some cases the antibody? proves difficult to get a large amount of positive cases and we make do with what we can get.? They are usually the read esoteric antibodies and you never find that many cases. 
? 
STP 
From LSebree <@t> uwhealth.org  Mon Sep 29 16:42:47 2014
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Mon Sep 29 16:42:51 2014
Subject: [Histonet] RE: Negative Control for Validation
In-Reply-To: <F362122314DD5C41BD407A39C4FCF8757B9C1E02@AKHV-EX2K12MB3.alaska.ihs.gov>
References: <F362122314DD5C41BD407A39C4FCF8757B9C1E02@AKHV-EX2K12MB3.alaska.ihs.gov>
Message-ID: <77DD817201982748BC67D7960F2F76AF0DE1C0@UWHC-MBX12.uwhis.hosp.wisc.edu>

Hey Karla,

We use Vimentin as a positive reagent control, our negative control serum for the negative reagent control and for the negative tissue control, the pathologist reviews the antibody stained slide and OK's the internal negative tissue elements as appropriately negative.

Hope that helps,

Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arrington, Karla A
Sent: Monday, September 29, 2014 1:25 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Negative Control for Validation

We are beginning the process of validating new antibodies using the BenchMark XT but have some questions for validation.

Question:  For a positive reagent control that stains appropriately positive, is a known control block used? If not, what is used?
Question:  For a negative reagent control that stains appropriately negative, what is ran?
Question:  Is a negative tissue slide ran for all of our 10 cases per antibody, or per case that will be used for validation?

Thanks All!
Karla

Karla Arrington, HT(ASCP), HIT(AHIMA)
Lead Histology Technician
ANMC Pathology
907-729-1810
kaarrington@anthc.org

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From dmaney22 <@t> yahoo.com  Mon Sep 29 20:51:30 2014
From: dmaney22 <@t> yahoo.com (Donna Maney)
Date: Mon Sep 29 20:51:40 2014
Subject: [Histonet] microtome stage
Message-ID: <1412041890.55883.YahooMailBasic@web125405.mail.ne1.yahoo.com>

I have a Leica SM2000 microtome, circa 2004, that has recently developed an issue with the dry ice tray. Up until this year, we've never had any problems with the specimen adhering to the platform. But suddenly we cannot cut specimens because they easily become dislodged. We use 30% sucrose to stick the specimen to the dry ice tray. When we borrow a dry ice tray from another lab, the problem goes away.

Our dry ice tray is powder coated and we can see the the coating is rubbing away, but it does not seem to be coming off with the specimen. Rotating the tray so that the specimen is attached at a different location on the platform does not help. We have cleaned the dry ice tray with hot soapy water, rinsed with distilled water and then with ethanol. 

Leica told me that the new trays do not have this powder coating, which makes me think they know about a problem, but the person I spoke to on the phone was not aware of one.

I'd be glad to hear from anyone who has experienced the same thing or who has suggestions. A new tray will cost $500-$1000 so I'd like to troubleshoot with this one to the extent that it is appropriate.

Thanks,
Donna

From latecor <@t> montevideo.com.uy  Mon Sep 29 21:46:36 2014
From: latecor <@t> montevideo.com.uy (Carlos Defeo)
Date: Mon Sep 29 21:46:16 2014
Subject: [Histonet] not receiving histonet mails
Message-ID: <201409292346360309.002F2ECB@smtp.montevideo.com.uy>

I am currently not receiving your imputs, so please be kind to inform if all it?s right with 
the listserver and my mail address.
My best wishes,
Carlos.-

From j.rowaihi <@t> alborglaboratories.com  Tue Sep 30 05:15:27 2014
From: j.rowaihi <@t> alborglaboratories.com (Jamal)
Date: Tue Sep 30 05:15:44 2014
Subject: [Histonet] RE: Negative Control for Validation
In-Reply-To: <77DD817201982748BC67D7960F2F76AF0DE1C0@UWHC-MBX12.uwhis.hosp.wisc.edu>
References: <F362122314DD5C41BD407A39C4FCF8757B9C1E02@AKHV-EX2K12MB3.alaska.ihs.gov>
	<77DD817201982748BC67D7960F2F76AF0DE1C0@UWHC-MBX12.uwhis.hosp.wisc.edu>
Message-ID: <000601cfdc97$76de3440$649a9cc0$@rowaihi@alborglaboratories.com>

Hi Linda
can you give us more details about the controls selection and processing
procedures.



Best Regards,


Jamal M. Al Rowaihi	Anatomic Pathology Supervisor       | Al Borg
Medical Laboratories |? Mobile +966 503629832|
j.rowaihi@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA    |
Phone: +966 12 670 0099	      | Fax: +966 12 676 4984     |
www.alborglaboratories.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda
A
Sent: Tuesday, September 30, 2014 12:43 AM
To: 'Arrington, Karla A'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Negative Control for Validation

Hey Karla,

We use Vimentin as a positive reagent control, our negative control serum
for the negative reagent control and for the negative tissue control, the
pathologist reviews the antibody stained slide and OK's the internal
negative tissue elements as appropriately negative.

Hope that helps,

Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arrington,
Karla A
Sent: Monday, September 29, 2014 1:25 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Negative Control for Validation

We are beginning the process of validating new antibodies using the
BenchMark XT but have some questions for validation.

Question:  For a positive reagent control that stains appropriately
positive, is a known control block used? If not, what is used?
Question:  For a negative reagent control that stains appropriately
negative, what is ran?
Question:  Is a negative tissue slide ran for all of our 10 cases per
antibody, or per case that will be used for validation?

Thanks All!
Karla

Karla Arrington, HT(ASCP), HIT(AHIMA)
Lead Histology Technician
ANMC Pathology
907-729-1810
kaarrington@anthc.org

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From LSebree <@t> uwhealth.org  Tue Sep 30 08:09:08 2014
From: LSebree <@t> uwhealth.org (Sebree Linda A)
Date: Tue Sep 30 08:09:13 2014
Subject: [Histonet] RE: Negative Control for Validation
In-Reply-To: <000601cfdc97$76de3440$649a9cc0$@rowaihi@alborglaboratories.com>
References: <F362122314DD5C41BD407A39C4FCF8757B9C1E02@AKHV-EX2K12MB3.alaska.ihs.gov>
	<77DD817201982748BC67D7960F2F76AF0DE1C0@UWHC-MBX12.uwhis.hosp.wisc.edu>
	<000601cfdc97$76de3440$649a9cc0$@rowaihi@alborglaboratories.com>
Message-ID: <77DD817201982748BC67D7960F2F76AF0DE2AA@UWHC-MBX12.uwhis.hosp.wisc.edu>

Jamal,

We use the same patient cases throughout the validation procedure.  So each block gets stained with the new antibody (optimized protocol already signed off by IHC director or designee), vimentin (+ reagent control) and negative control serum (- reagent control).  As stated earlier, the negative tissue control is any non-staining negative tissue elements, expected to be negative, within the block.  

If you're interested I can send you our Validation Protocol Proposal form under separate cover.

Linda

Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 


-----Original Message-----
From: Jamal [mailto:j.rowaihi@alborglaboratories.com] 
Sent: Tuesday, September 30, 2014 5:15 AM
To: Sebree Linda A; 'Arrington, Karla A'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Negative Control for Validation

Hi Linda
can you give us more details about the controls selection and processing procedures.



Best Regards,


Jamal M. Al Rowaihi	Anatomic Pathology Supervisor       | Al Borg
Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA    |
Phone: +966 12 670 0099	      | Fax: +966 12 676 4984     |
www.alborglaboratories.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Tuesday, September 30, 2014 12:43 AM
To: 'Arrington, Karla A'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Negative Control for Validation

Hey Karla,

We use Vimentin as a positive reagent control, our negative control serum for the negative reagent control and for the negative tissue control, the pathologist reviews the antibody stained slide and OK's the internal negative tissue elements as appropriately negative.

Hope that helps,

Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory
600 Highland Ave. 
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arrington, Karla A
Sent: Monday, September 29, 2014 1:25 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Negative Control for Validation

We are beginning the process of validating new antibodies using the BenchMark XT but have some questions for validation.

Question:  For a positive reagent control that stains appropriately positive, is a known control block used? If not, what is used?
Question:  For a negative reagent control that stains appropriately negative, what is ran?
Question:  Is a negative tissue slide ran for all of our 10 cases per antibody, or per case that will be used for validation?

Thanks All!
Karla

Karla Arrington, HT(ASCP), HIT(AHIMA)
Lead Histology Technician
ANMC Pathology
907-729-1810
kaarrington@anthc.org

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From numberfife <@t> gmail.com  Tue Sep 30 08:35:51 2014
From: numberfife <@t> gmail.com (Stephanie Moore)
Date: Tue Sep 30 08:36:05 2014
Subject: [Histonet] From: Stephanie Moore
Message-ID: <nkqu4e4ur57ye4td3uubnznm.14120841511809@email.android.com>


Hi histonet



http://buymobile.kz/tail.php?cool=qzmvknxxxw3181cnp


Stephanie Moore
From relia1 <@t> earthlink.net  Tue Sep 30 09:50:43 2014
From: relia1 <@t> earthlink.net (Pam Barker)
Date: Tue Sep 30 09:50:50 2014
Subject: [Histonet] RELIA Special Histology Job Alert 9-30-2014. First Look
	at Some of the Best Opportunities this Fall!!
Message-ID: <09ca01cfdcbd$e96edc00$bc4c9400$@earthlink.net>

Hi Histonetters!!
How are you doing today? 
Are you enjoying a fun filled Fall?  
For me the Summer just flew by!!  
I have been so busy here at RELIA.   
I have made a lot of histotechs happy with the positions
we landed for them this summer!!
I am sending this special alert out because I am starting the Fall just like
I started the Summer: 
WITH SOME OF THE BEST JOB OPPORTUNITIES OUT THERE!!!

I am especially excited about these openings because they are with several
of my best clients.  Why do I refer to them as best clients . Well for a
variety of reasons:  
Location, 
The rest of the team,
The fantastic manager, 
Excellent benefits, 
Generous relocation 
And most importantly I like a client that only has openings due to growth. 

Here is a list of the positions that I am most excited about:
Seattle, WA - Director of Safety Assessment
Hammond, IN - AP Manager
Hammond, IN - Histotechnician
Austin, TX - Histotechnician
Austin, TX - IHC Specialist
Fayetteville, AR - Dermpath Histotech 
Norfolk, VA - Histotechnologist - 15K Sign On Bonus!
Philadelphia, PA - Histotechnician
Lafayette, LA - Cytotech
Wilmington, NC - Cytotech

If you or anyone you know is interested in more details   I can be reached
toll free at 866-607-3542 or relia1@earthlink.net. 
If you are starting to save up your Christmas money remember I pay a
referral fee to you if I place someone that you refer to me.  

Have a Great Day!!

Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1@earthlink.net 
www.facebook.com <http://www.facebook.com/PamBarkerRELIA> /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 





From Karoleigh.Armstrong <@t> ARMC.net  Tue Sep 30 13:04:51 2014
From: Karoleigh.Armstrong <@t> ARMC.net (Armstrong, Karoleigh T)
Date: Tue Sep 30 13:05:45 2014
Subject: [Histonet] Glacial Acetic Acid subs.
Message-ID: <A896A4059BD1884BA0788CF01B17FA6EE3AA01@TN001WEXMBX015.US.chs.net>

Does any one know of a substitute for Glacial Acetic Acid? We use it to clean the lines on the VIP and in the Eosin. Our Corp. heads want to eliminate Glacial in concentration form from all its hospitails.
Thanks,
Karoleigh Armstrong, HT (ASCP)


--------------------------------------------------------------------------
Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains.
From abright <@t> brightinstruments.com  Tue Sep 30 14:35:46 2014
From: abright <@t> brightinstruments.com (Alan Bright)
Date: Tue Sep 30 14:35:54 2014
Subject: [Histonet] microtome stage
In-Reply-To: <1412041890.55883.YahooMailBasic@web125405.mail.ne1.yahoo.com>
References: <1412041890.55883.YahooMailBasic@web125405.mail.ne1.yahoo.com>
Message-ID: <07B23E14-1D4D-4067-A643-0313D9BB22B0@brightinstruments.com>

Donna, I would take the powder coating off with a paint stripper, try sectioning, if happy have the holder electro plated which will be a conductive coating that will protect the base metal from corrosion. Cost under $20.
BR........ Alan Bright, BIC...Cambs..UK

Sent from my iPhone

> On 30 Sep 2014, at 02:53, Donna Maney <dmaney22@yahoo.com> wrote:
> 
> I have a Leica SM2000 microtome, circa 2004, that has recently developed an issue with the dry ice tray. Up until this year, we've never had any problems with the specimen adhering to the platform. But suddenly we cannot cut specimens because they easily become dislodged. We use 30% sucrose to stick the specimen to the dry ice tray. When we borrow a dry ice tray from another lab, the problem goes away.
> 
> Our dry ice tray is powder coated and we can see the the coating is rubbing away, but it does not seem to be coming off with the specimen. Rotating the tray so that the specimen is attached at a different location on the platform does not help. We have cleaned the dry ice tray with hot soapy water, rinsed with distilled water and then with ethanol. 
> 
> Leica told me that the new trays do not have this powder coating, which makes me think they know about a problem, but the person I spoke to on the phone was not aware of one.
> 
> I'd be glad to hear from anyone who has experienced the same thing or who has suggestions. A new tray will cost $500-$1000 so I'd like to troubleshoot with this one to the extent that it is appropriate.
> 
> Thanks,
> Donna
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> -- 
> 

From JMacDonald <@t> mtsac.edu  Tue Sep 30 15:05:24 2014
From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald)
Date: Tue Sep 30 15:05:33 2014
Subject: [Histonet] Glacial Acetic Acid subs.
In-Reply-To: <A896A4059BD1884BA0788CF01B17FA6EE3AA01@TN001WEXMBX015.US.chs.net>
References: <A896A4059BD1884BA0788CF01B17FA6EE3AA01@TN001WEXMBX015.US.chs.net>
Message-ID: <OFD0E8E099.72D5E517-ON88257D63.006E4FA4-88257D63.006E27D2@mtsac.edu>

vinegar.  you can buy "cleaning" vinegar.  It is acetic acid, but not 
concentrated.



From:   "Armstrong, Karoleigh T" <Karoleigh.Armstrong@ARMC.net>
To:     "histonet@lists.utsouthwestern.edu" 
<histonet@lists.utsouthwestern.edu>
Date:   09/30/2014 11:09 AM
Subject:        [Histonet] Glacial Acetic Acid subs.
Sent by:        histonet-bounces@lists.utsouthwestern.edu



Does any one know of a substitute for Glacial Acetic Acid? We use it to 
clean the lines on the VIP and in the Eosin. Our Corp. heads want to 
eliminate Glacial in concentration form from all its hospitails.
Thanks,
Karoleigh Armstrong, HT (ASCP)


--------------------------------------------------------------------------

Disclaimer: This electronic message may contain information that is 
Proprietary, Confidential, or legally privileged or protected. It is 
intended only for the use of the individual(s) and entity named in the 
message. If you are not an intended recipient of this message, please 
notify the sender immediately and delete the material from your computer. 
Do not deliver, distribute or copy this message and do not disclose its 
contents or take any action in reliance on the information it contains.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Mari.Yang <@t> Hoag.org  Tue Sep 30 15:43:15 2014
From: Mari.Yang <@t> Hoag.org (Yang, Mari)
Date: Tue Sep 30 15:43:24 2014
Subject: [Histonet] Pathologist Assistant in Newport Beach, CA
Message-ID: <02A9B6BBE70204458C12BF79FEEF018C0F90F69E@HHEXMB03.hoag.org>

Dear Histonet,

 

I am pleased to announce a Pathologist Assistant position for Hoag
Hospital, located in Newport Beach, CA is now available. All candidates
PA(ASCP) certified or eligible are encouraged to apply. For further
information, please visit http://www.hoag.org/Pages/Hoag-Careers.aspx
and search pathologist assistant.



Regards,

Mari

 

Mari Yang, MHA, CTCM(ASCP)HTLCM

Manager | Anatomic Pathology & Cytology

Hoag Memorial Presbyterian Hospital

One Hoag Drive, PO Box 6100, Newport Beach, CA 92658

949.764.5631 | Mari.Yang@hoag.org

 

P Save a tree, please don't print this e-mail unless you really need to.

 

Confidentiality Note: The preceding e-mail message (including any
attachments) contains information that may be confidential, protected by
applicable legal privileges, or constitute non-public information. It is
intended to be conveyed only to the designated recipient(s). If you are
not an intended recipient of this message, please notify the sender by
replying to this message and then delete it from your system. Use,
dissemination, distribution or reproduction of this message by
unintended recipients is not authorized and may be unlawful. 

 


Please note that the information contained in this message and any files
transmitted with it are privileged and confidential and are
protected from disclosure under the law, including the Health Insurance
Portability and Accountability Act (HIPAA). If the reader of this
message is not the intended recipient, or an employee or agent
responsible for delivering this message to the intended recipient, you
are hereby notified that any dissemination, distribution or copying of
this communication is strictly prohibited and may subject you to
criminal or civil penalties. If you have received this communication in
error, please notify the sender by replying to the message and delete
the material from any computer. 

Thank you, 

Hoag Memorial Hospital Presbyterian and its Affiliates

From drmoses111 <@t> comcast.net  Tue Sep 30 19:40:17 2014
From: drmoses111 <@t> comcast.net (drmoses111@comcast.net)
Date: Tue Sep 30 19:40:46 2014
Subject: [Histonet] myogenin control tissue
In-Reply-To: <1763476501.38021318.1412122940104.JavaMail.root@comcast.net>
Message-ID: <1635676488.38031735.1412124017619.JavaMail.root@comcast.net>

Does anyone have a control block for myogenin? My only block of rhabdomyosarcoma is nearly exhausted. Please contact me at drmoses111@comcast.net,, 
Thank you, 
Renee Boston-Moses HT,QIHC 
Virtua Health System 
Histology Department 
100 Bowman Dr. 
Voorhees, NJ 08043