[Histonet] Re: squamous cell contamination on slides

[Margaryan, Naira]

Hi Hestonetters,

I need to do IHC of SC4MOL on FFPE sections.
If any of you have been done this staining before, please send me your procedure.

Thanks in advance,
Naira



Ranked nationally in all 10 pediatric specialties by U.S. News & World Report  (LCHOC Ver 1.0)


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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of gayle callis
Sent: Wednesday, October 29, 2014 1:48 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: squamous cell contamination on slides

After following this thread on a topic that has always been a pet peeve and a problem at times, a few suggestions. This is a problem discussed many times on Histonet.



If people are using the water bath as a "finger bowl", they need to learn to not let fingers go "snorkeling" when picking up sections.  This is sloppy, poor technique.  Anyone can learn to never touch the water surface with bare skin. I have seen people immerse their fingers up to the first joint and their stained sections were covered by a snowstorm of squamous cells.



Be aware that squamous cells are going to exfoliate from other than hands, so not touching face and hair is good advice even if one wears gloves.



Hand lotion is helpful except for those slather on lotion or the lotion is particularly heavy duty and then they still touch the water. This can cause a double problem - an oil slick which is a terrible section adhesive along with squamous cells from bare skin.



Hold slide at top or on sides, as mentioned previously.

Wear gloves. Not always popular with a common argument one loses dexterity handling slides.  If gloves do not flop around loosely but fit the hand well, then dexterity is not lost.



Good luck



Gayle Callis

HTL/HT/MT(ASCP)



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We have that problem mostly during the winter months when our hands get dry.
Use hand lotion, that usually helps a bit.



Thanks,



Michele Margiotta-Watz

Histology Supervisor

BMHMC

101 Hospital Rd.

Patchogue, NY 11772

631-654-7192



-----Original Message-----

From:  <http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber McKenzie

Sent: Tuesday, October 28, 2014 3:55 PM

To:  <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> histonet <@t> lists.utsouthwestern.edu

Subject: [Histonet] Squamous cells staining on H&E and IHC





Does anyone else have problems with what looks like squamous cells staining on your H&E's and IHC's?  I'm trying to figure out how to eliminate that problem in our lab...wear gloves while cutting?  Change out water bath several times during shifts? Any suggestions?  Thanks!



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