[Histonet] RE: Squamous cells staining on H&E and IHC

[Michael Ann Jones]

We had this issue - particularly for our Keratin stain (as it highlights
the squamous cells) We had much positive staining debris scattered on our
slides.
We cleaned our water baths diligently, made sure our techs were skimming
between slides, and air current and stainers can bring debris as well. We
kind of narrowed it down to one tech maybe? She got frustrated and doesn¹t
cut keratin controls anymore.
Sadly, I¹m not sure how/why this problem subsided. Following this
discussion closely.

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones <@t> metropath.com




On 10/29/14, 4:44 AM, "Pathology-Histology Sr. Supervisor"
<histo <@t> skm.org.pk> wrote:

>When the sections are cut and taken on the slide from water bath, slide
>must only holds on the frosted end of the slides don't touch other part
>of slide.
>Regards
>Muhammad Tahseen
>MLT (JIMTEF) Japan
>Histology Supervisor
>SKMCH&RC
>Lahore
>Pakistan
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber
>McKenzie
>Sent: Wednesday, October 29, 2014 12:55 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Squamous cells staining on H&E and IHC
>
>
>Does anyone else have problems with what looks like squamous cells
>staining on your H&E's and IHC's?  I'm trying to figure out how to
>eliminate that problem in our lab...wear gloves while cutting?  Change
>out water bath several times during shifts? Any suggestions?  Thanks!
>
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