[Histonet] RE: Squamous cells staining on H&E and IHC

[Pathology-Histology Sr. Supervisor]

When the sections are cut and taken on the slide from water bath, slide must only holds on the frosted end of the slides don't touch other part of slide.
Regards
Muhammad Tahseen
MLT (JIMTEF) Japan
Histology Supervisor
SKMCH&RC
Lahore
Pakistan

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Wednesday, October 29, 2014 12:55 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Squamous cells staining on H&E and IHC


Does anyone else have problems with what looks like squamous cells staining on your H&E's and IHC's?  I'm trying to figure out how to eliminate that problem in our lab...wear gloves while cutting?  Change out water bath several times during shifts? Any suggestions?  Thanks!

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