[Histonet] fish eggs: processing and fixation

[Thompson, Christy]

Sadly we don't have the capacity to do  frozen sections :(

Christy

-----Original Message-----
From: Diane G. Miller [mailto:dgmiller <@t> coho.net] 
Sent: October 23, 2014 11:56 AM
To: Thompson, Christy
Subject: Re: [Histonet] fish eggs: processing and fixation

Have you tried doing frozen sections instead of processing them in
paraffin. 
We use to do frozen sections on frog eggs that were fixed and it worked
picking them up on charged slides.
Diane

-----Original Message-----
From: Thompson, Christy
Sent: Thursday, October 23, 2014 11:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] fish eggs: processing and fixation

Hi all, I'm currently working on some unfertilized fish eggs. They were
fixed in 10% NBF and processed in a vacuum processor with paraffin. The
eggs are extremely hard to section and most of the yolk goes AWOL on the
way to the water bath.  I did do a search here for some help and a few
things were posted but one post in particular found some answers but
they were not listed in his reply and I think the post was pretty old
(but no date on it).  Anyone out there have any success with sectioning
fish eggs?
Thanks in advance!

Christy Thompson
A/Histology Lab Leader - Aquatic Animal Health Pacific Biological
Station Fisheries and Oceans Canada Nanaimo, BC
250-756-7060
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