[Histonet] Double Labeling EM Blocks

[koellingr <@t> comcast.net]

Neelam, 
If you are referring to immunolabelling for transmission electron microscopy, it is relatively simple to apply and this worked wonderfully for projects we did, to use 2 antibodies to two different epitopes but each antibody with a different size gold particle attached.  The gold particles are between a few nanometers up to 30 or 40 nanometers in diameter which are attached to antibodies and so are easy to tell apart from one another. 
  
Ray 
Lake Forest Park, WA 

----- Original Message -----

From: "Neelam Joshi" <Neelam.Joshi <@t> nephropath.com> 
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Thursday, October 23, 2014 5:42:21 AM 
Subject: [Histonet] Double Labeling EM Blocks 

Hello, 

Does anyone has a procedure for double labeling EM Blocks with two identifier? 

Please let me know as soon as possible. 

Thanks you all! 

Neelam Joshi,HT 
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