[Histonet] RE: Histonet Digest, Vol 131, Issue 22 ] Reticle Stain

Mon Oct 20 12:28:31 CDT 2014

I agree with Rebecca. We had this problems constantly at the previous hospital I worked at and were in contact with Ventana frequently regarding this issue. It is my understanding that this hospital is still having problems and that a Ventana rep is in there frequently to perform decons. It's all about the decons! Very frustrating! Where I am now, we hand stain our retics, no problems!! Good luck!

Tanya G. Abbott
Histology/Cytology
St. Joseph Medical Center
610-378-2635

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Subject: Histonet Digest, Vol 131, Issue 22

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Today's Topics:

   1. Staining bacteria in cryo + paraffin (Mikael Niku)
   2. Dallas job openings (Michelle Lamphere)
   3. Protargol S (Freida Carson)
   4. Re: Protargol S (Tina Van Meter)
   5. RE: Histonet Digest, Vol 131, Issue 20 (Riesen, Rebecca)
   6. Mount-Quick for tissue transfer? (Eva Permaul)
   7. Re: Protargol S (Rene J Buesa)

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Message: 1
Date: Mon, 20 Oct 2014 14:00:11 +0300
From: Mikael Niku <mikael.niku <@t> helsinki.fi>
Subject: [Histonet] Staining bacteria in cryo + paraffin
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <5444EB3B.5050909 <@t> helsinki.fi>
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Hello!

What is your preferred method to show ANY bacteria (mostly intestinal
flora) on cryo AND paraffin sections? Two different stains OK.

With best regards,
Mikael

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Message: 2
Date: Mon, 20 Oct 2014 13:08:35 +0000
From: Michelle Lamphere <MICHELLE.LAMPHERE <@t> childrens.com>
Subject: [Histonet] Dallas job openings
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
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We have full and part time openings at Children's Health in Dallas, TX.  Positions require qualification for High Complexity Testing.  Please contact me directly to inquire.

Michelle Lamphere
Senior Tech, Histology
Children's Health
214-456-2318
michelle.lamphere <@t> childrens.com

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Message: 3
Date: Mon, 20 Oct 2014 08:43:00 -0500
From: Freida Carson <freidac <@t> sbcglobal.net>
Subject: [Histonet] Protargol S
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
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Does anyone have a source for good protargol for Bodian stains? A friend in Sweden is asking.

Sent from my iPhone

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Message: 4
Date: Mon, 20 Oct 2014 10:02:45 -0400
From: Tina Van Meter <tina.vanmeter <@t> gmail.com>
Subject: Re: [Histonet] Protargol S
To: Freida Carson <freidac <@t> sbcglobal.net>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
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???Hi Freida,
American MasterTech has a Bodian Stain Kit #KC245Z,  $48.00

Kind regards,
Tina

Tina Van Meter, HT (ASCP)
Histology Core Manager
Scripps Research Institute
Jupiter, FL

On Mon, Oct 20, 2014 at 9:43 AM, Freida Carson <freidac <@t> sbcglobal.net>
wrote:

> Does anyone have a source for good protargol for Bodian stains? A 
> friend in Sweden is asking.
>
> Sent from my iPhone
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Message: 5
Date: Mon, 20 Oct 2014 14:57:36 +0000
From: "Riesen, Rebecca" <Rebecca.Riesen <@t> hma.com>
Subject: [Histonet] RE: Histonet Digest, Vol 131, Issue 20
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
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We had this issue and found that there was contamination in the lines and buffer of the Benchmark.  After working with Ventana it was noticed that "big" labs that went through their kits quickly were not experiencing the problem, but us "little-guys" had an issue after a certain period or time (which was determined by how quickly each lab would exhaust a kit)  We ended up with a Ventana tech having to come do extra decontaminations on our instrument almost every other week followed by preparing new buffer solution.  It only seemed to happen on the Silver Stains.  I believe Ventana is working on a solution to the problem, but I'm unsure of where they are in the process. Sorry, I don't have a fix, other than extra decontaminations.  

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Message: 14
Date: Fri, 17 Oct 2014 01:54:57 -0700
From: Bharti Parihar <bhartologist <@t> gmail.com>
Subject: [Histonet] Reticle Stain
To: histonet <@t> lists.utsouthwestern.edu
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Anyone know how to troubleshoot this Retic issue? The stain result is not the usual continuous silver stain reticulin fibers. Instead the fibers are more like dashed lines and the fibers are not visible throughout the entire tissue, especially edges.  Ventana benchmark machines being used with their reagents.  Thought maybe it was a reagent lot issue so tried different lot as well as different machine and getting similar results.  Could it be the liver control we're using? Haven't had any issues with our tissue used as controls in the past for this stain. Any help on this will be appreciated!
Thanks!

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Message: 6
Date: Mon, 20 Oct 2014 11:13:40 -0400
From: Eva Permaul <eca9 <@t> georgetown.edu>
Subject: [Histonet] Mount-Quick for tissue transfer?
To: histonet <histonet <@t> lists.utsouthwestern.edu>
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Good morning Histonetters,
I was wondering if anyone has used Mount-Quick to transfer tissue from uncharged slides to charged slides. We had a submitter give us slides for staining on uncharged slides and the tissue comes off the slide during antigen retrieval. I remember reading about this product but have never used it. Is it hard to do? Are the slides staining well afterwards?
Thank you,
Eva
Georgetown University

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Message: 7
Date: Mon, 20 Oct 2014 15:29:08 +0000 (UTC)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Protargol S
To: Freida Carson <freidac <@t> sbcglobal.net>, 	Histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<1649348788.286357.1413818948621.JavaMail.yahoo <@t> jws100187.mail.ne1.yahoo.com>

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Protargol, as you well know, oxidizes very easily after you open the container, so I??prepared my own protargol every time I needed it and the results were always consistent and of great quality.Under separate cover I am forwarding you??an article??I wrote on the JoH on the subject.Ren?? J.?? 

     On Monday, October 20, 2014 9:43 AM, Freida Carson <freidac <@t> sbcglobal.net> wrote:

 Does anyone have a source for good protargol for Bodian stains? A friend in Sweden is asking.

Sent from my iPhone
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End of Histonet Digest, Vol 131, Issue 22
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