[Histonet] RE: Histonet Digest, Vol 131, Issue 20

[Riesen, Rebecca]

We had this issue and found that there was contamination in the lines and buffer of the Benchmark.  After working with Ventana it was noticed that "big" labs that went through their kits quickly were not experiencing the problem, but us "little-guys" had an issue after a certain period or time (which was determined by how quickly each lab would exhaust a kit)  We ended up with a Ventana tech having to come do extra decontaminations on our instrument almost every other week followed by preparing new buffer solution.  It only seemed to happen on the Silver Stains.  I believe Ventana is working on a solution to the problem, but I'm unsure of where they are in the process. Sorry, I don't have a fix, other than extra decontaminations.  

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Message: 14
Date: Fri, 17 Oct 2014 01:54:57 -0700
From: Bharti Parihar <bhartologist <@t> gmail.com>
Subject: [Histonet] Reticle Stain
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<CAJxUErjEVP3YXE4oE1pbadMbw-MQ0x1D8xgSY3q_PpN1jFCYgQ <@t> mail.gmail.com>
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Anyone know how to troubleshoot this Retic issue? The stain result is not
the usual continuous silver stain reticulin fibers. Instead the fibers are
more like dashed lines and the fibers are not visible throughout the entire
tissue, especially edges.  Ventana benchmark machines being used with their
reagents.  Thought maybe it was a reagent lot issue so tried different lot
as well as different machine and getting similar results.  Could it be the
liver control we're using? Haven't had any issues with our tissue used as
controls in the past for this stain. Any help on this will be appreciated!
Thanks!



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