[Histonet] re:Mouse FFPE IHC double stain caspase-3 and Ki67 (Melanie Smith)

[Hobbs, Carl]

Firstly, take a piece of tissue that clearly shows Ki67/CC3, such as small intestine.
Do your std fix and processing.
Also.....take another piece,  fix and decalcify identically to your kneejoints.
Then process  the same way.
Optimise  both Abs independently, on the NON-decalcified gut sections.
Once optimised​, repeat on the decalcified gut.
If the latter works.....then so should double-labelling then...your kneejoints!

Does this make sense?

Best regards

carl



  
 
Carl Hobbs FIBMS 
Histology and Imaging Manager 
Wolfson CARD 
Guys Campus, London Bridge  
Kings College London 
London 
SE1 1UL 
  
020 7848 6813