[Histonet] re:Mouse FFPE IHC double stain caspase-3 and Ki67
(Melanie Smith)
Hobbs, Carl
carl.hobbs <@t> kcl.ac.uk
Wed Oct 8 21:51:29 CDT 2014
Firstly, take a piece of tissue that clearly shows Ki67/CC3, such as small intestine.
Do your std fix and processing.
Also.....take another piece, fix and decalcify identically to your kneejoints.
Then process the same way.
Optimise both Abs independently, on the NON-decalcified gut sections.
Once optimised, repeat on the decalcified gut.
If the latter works.....then so should double-labelling then...your kneejoints!
Does this make sense?
Best regards
carl
Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL
020 7848 6813
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