[Histonet] alizarin red s solution -query

[raghulj <@t> orchidpharma.com]

Dear all,

We stained decalcified paraffin sections of rodent femur by alizarin red S using the following protocol -

a. deparrafinised in xylene
b. 100%ethaonol->95% ethaonol-->70% ethaonol-->distillled water.
c. dipped in 2%alizarin red S, dissolved in distilled water (pH adjusted with 12% NH4OH to 4.26) for 2-5min
d. acetone (20dips)
e. aceteon: xylene (20dips)
f. three changes of xylene and mounted with dpx

The alizarin solution turned more colloidal during the staining process. However we went ahead staining few de paraffin slides rodent femurs.

Is it in the nature of alizarin solution turning colloidal? The staining of the paraffin sections were satisfactory. Being, the first time  the solution turning colloidal was surprising.

Kindly suggest.

J.Raghul
Senior Research Associate

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