[Histonet] Free Webinar: Avoid Errors in Quantification

[Jerry Sedgewick]

For those of you who quantify optical densities of DAB, IP, etc.; and for
those who measure for areas, counts, etc (morphometry); please read below
regarding a free webinar.  Note that the webinar will present a new product
and software solution.  I encourage you to listen, even if you are not in a
position to purchase this product/software solution, because you will learn
about linearity, the importance of linear tones when performing optical
density measurements, and the possibility that your camera system is not
producing linear images and is, therefore, providing an image that contains
erroneous data.
Morphometry and Density/Intensity Readings: Avoid Errors in Quantification
------------------------------
 Tuesday, October 21, 2014 1:00 PM - 1:30 PM EDT Webinar Registration:
https://www2.gotomeeting.com/register/945357514

Introduction by Michael Linden, MD, PhD
(Assistant Professor, Laboratory Medicine and Pathology, University of
Minnesota, Minneapolis, MN)

Presenter: Jerry Sedgewick
(Imaging and Analysis, LLC; author, consultant and recognized authority in
post-processing, analysis and quantification of scientific images)


If you have done quantitation, you know that inconsistent images require
subjective means for setting thresholds when segmenting objects for
measurement. You need an objective method to set thresholds for measurement
of area, length, count, etc., and be able to apply that method across
multiple images.

When quantifying densities and intensities, it is imperative that images
contain equal increments of grey or color values across the dynamic range
(image linearity). Can you trust your images? A recent survey found that
approximately 50% of color camera systems offer software settings that can
result in non-linear color images. If you’ve been quantifying immunostained
cells or cellular structures (i.e. DAB, BCIP/NBT, X-Gal, etc.), you may
have presented false data.

This webinar explores quantitative methods that employ a single threshold
for any number of color brightfield images. You will learn how to create
consistent images, without subjective adjustments, for subsequent
quantization. You will learn how to confirm your imaging system delivers
scientific images that are linear. You will also learn how to correct
images taken over a time course while keeping tones and colors linear.


-- 
Jerry (Gerald) Sedgewick
quickphotoshop.com
http://www.imagingandanalysis.com
Author: "Scientific Imaging with Photoshop: Methods, Measurement and Output"

Automated Image Measurement services and systems; On-Site and on-line
training for image acquisition/processing/quantitation in science,
microscopy and medicine.