[Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

Arun Jyothi S.P arunjyothisp <@t> gmail.com
Wed Nov 26 00:28:59 CST 2014

Dear all
We find the problem
The retort was over heating and thats y the condensation also very high
When the condensation chamber is full its pumping back the reagent in the
condensation chamber to the retort and mixing it with paraffin
Thanks for all the support
On 26 Nov 2014 02:39, "Tony Henwood (SCHN)" <tony.henwood <@t> health.nsw.gov.au>

> Also check the last alcohol and xylene.
> Has someone accidentally replaced these solution with formalin by mistake?
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Laboratory Manager & Senior Scientist, the Children’s Hospital at Westmead
> Adjunct Fellow, School of Medicine, University of Western Sydney
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
> Sent: Wednesday, 26 November 2014 5:17 AM
> To: Arun Jyothi S.P; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Formalin smell in the last paraffin station in vip
> 6 tissue tek
> Not sure if it's the same scenario, but this happened to us once about a
> dozen years ago on an older version of a VIP.  Luckily, I haven't seen this
> again!
> One of our lab assistants loaded a basket of formalin-fixed tissues onto a
> processor that hadn't yet been through the clean cycle.  The machine
> wouldn't let him start the process, so he took the basket out and put it
> back into formalin to wait while he ran the clean cycle.  For that brief
> moment that the basket was in the retort, a bunch of residual formalin
> drained off the tissues and basket and mixed in with the residual molten
> paraffin that was left in the bottom of the retort.  Anyway, after he ran
> the clean cycle he started the run as usual.  8 hours later, our blocks
> smelled like formalin when we cut them, and they were all "mushy" in
> texture.
> So Sakura told us that whenever you run the clean cycle, the VIP (again,
> it was a really old model and I'm not sure if the technology is still the
> same) will attempt to draw back into the last station that was used, any
> residual reagent that was left in the retort before proceeding to the next
> reagent.  Since the last reagent had been paraffin, anything in the retort
> (including the newly mixed in formalin) went back into the last paraffin
> chamber on the VIP.  As such, the next basket of tissues were infiltrated
> with formalin-infused paraffin in the very last processing step.   If I
> recall, we had to reprocess almost all of those blocks.
> Hope this helps.
> Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of
> Pathology and Laboratory Medicine Children's Hospital Los Angeles
> bcooper <@t> chla.usc.edu
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P
> Sent: Monday, November 24, 2014 10:53 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Formalin smell in the last paraffin station in vip 6
> tissue tek
> Dear all
> After processing in vip 6 the last paraffin has a strong odour of formalin
> Have anybody experienced the same
> Any ideas why it is happening.
> Arun
> Kuwait
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