[Histonet] Fast Green

Jay Lundgren jaylundgren <@t> gmail.com
Fri Nov 21 15:15:12 CST 2014

     Try 70% ETOH, leave the slides to soak for a good while.  If that
doesn't work after a couple of hours, try 1% HCl in 70% ETOH.

     Potassium permanganate followed by oxalic acid takes out just about
anything, in my experience, but it's pretty harsh (1% aq potassium
permanganate, 2% aq oxalic acid).  Just like cleaning a stain on a shirt,
you always want to start with the weakest solution first, and work your way
up if necessary.


                                             Jay A. Lundgren, M.S., HTL

On Fri, Nov 21, 2014 at 12:49 PM, Berger, Rebecca <BergerR1 <@t> email.chop.edu>

> Hey there, does anyone have a good method to de-stain Fast green? The
> slides need to be re-stained with H&E.
> Thanks.
> Rebecca Berger, HT(ASCP)CM
> Research Technician
> Division of Orthopedic Surgery
> The Children's Hospital of Philadelphia Research Institute
> 3615 Civic Center Boulevard, ARC904 (Lab)
> Philadelphia, PA 19104
> Phone: 267-425-2076  Email: bergerr1 <@t> email.chop.edu<mailto:
> bergerr1 <@t> email.chop.edu>
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