[Histonet] RE: lab math: ihc dilution

[Elizabeth Chlipala]

Sheryl

I will add another bit of information.  That final antibody protein concentration is very low.  Most of our antibodies (around 60 to 70%) will be in the range of 1 to 10 ug/ml.  We do have some antibodies that can have some very low protein concentration though such as neurofilament.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
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liz <@t> premierlab.com
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl
Sent: Friday, November 21, 2014 12:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] lab math: ihc dilution

To all you math whiz out there, please help with this math dilution.

If my Ab conc is 1.56 mg/mL and they want to optimize to 0.8 ug/mL, what dilution should I use?


Thanks,

Sheryl 




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